专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

EP0444639A2 A gene encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant 失效
标题翻译：编码具有多肽腈水合酶活性的基因，含有该转化体和方法用于制备酰胺的与该转化的基因。
公开(公告)号：EP0444639A2
公开(公告)日：1991-09-04
申请号：EP91102936.1
申请日：1991-02-27
申请人： NITTO CHEMICAL INDUSTRY CO., LTD. , Beppu, Teruhiko , YAMADA, Hideaki
发明人： Beppu, Teruhiko , Yamada, Hideaki , Nagasawa, Toru , Horinouchi, Sueharu , Nishiyama, Makoto
IPC分类号： C12N15/55 , C12N9/80 , C12P13/02
CPC分类号： C12N9/80 , C12P13/02
摘要： The present invention relates to a gene derived from Pseudomonas chlororaphis B23 strain which encodes a polypeptide having nitrile hydratase activity being capable of hydrating nitriles to amides. The invention also relates to a recombinant DNA containing the gene, and a transformant transformed with the recombinant DNA. The present invention further relates to a method of producing nitrile hydratase using the transformant and of amides using nitrile hydratase.
摘要翻译：本发明涉及由假单胞菌来源的基因chlororaphis B23株，其编码的多肽具有腈水合酶活性能够水化腈酰胺。因此，本发明涉及含有该基因的重组DNA，并用该重组DNA转化的转化体。本发明还涉及使用该转化体生产腈水合酶的和使用的腈水合酶的酰胺的方法。

2. 发明授权

EP0444639B1 A gene encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant 失效
标题翻译：编码具有多肽腈水合酶活性的基因，所述含转化子的基因，和用于制备该转化体的酰胺的方法。
公开(公告)号：EP0444639B1
公开(公告)日：1996-01-31
申请号：EP91102936.1
申请日：1991-02-27
申请人： NITTO CHEMICAL INDUSTRY CO., LTD. , Beppu, Teruhiko , YAMADA, Hideaki
发明人： Beppu, Teruhiko , Yamada, Hideaki , Nagasawa, Toru , Horinouchi, Sueharu , Nishiyama, Makoto
IPC分类号： C12N15/55 , C12N9/80 , C12P13/02
CPC分类号： C12N9/80 , C12P13/02

3. 发明公开

EP0444639A3 A gene encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant 失效
标题翻译：编码具有NITRILE HYDRATASE活性的多肽的基因，含有该基因的转化体和使用变体的生产氨基酸的方法
公开(公告)号：EP0444639A3
公开(公告)日：1992-07-08
申请号：EP91102936.1
申请日：1991-02-27
申请人： NITTO CHEMICAL INDUSTRY CO., LTD. , Beppu, Teruhiko , YAMADA, Hideaki
发明人： Beppu, Teruhiko , Yamada, Hideaki , Nagasawa, Toru , Horinouchi, Sueharu , Nishiyama, Makoto
IPC分类号： C12N15/55 , C12N9/80 , C12P13/02
CPC分类号： C12N9/80 , C12P13/02
摘要： The present invention relates to a gene derived from Pseudomonas chlororaphis B23 strain which encodes a polypeptide having nitrile hydratase activity being capable of hydrating nitriles to amides. The invention also relates to a recombinant DNA containing the gene, and a transformant transformed with the recombinant DNA. The present invention further relates to a method of producing nitrile hydratase using the transformant and of amides using nitrile hydratase.

4. 发明公开

EP0502476A3 Hybrid plasmid vectors containing genes encoding nitrile degrading enzymes and methods of producing amides and acids 失效
标题翻译：包含编码NITRILE降解酶的基因的混合载体载体和生产酰胺和酸的方法
公开(公告)号：EP0502476A3
公开(公告)日：1993-04-21
申请号：EP92103610.9
申请日：1992-03-03
申请人： NITTO CHEMICAL INDUSTRY CO., LTD. , Beppu, Teruhiko
发明人： Beppu, Teruhiko , Horinouchi, Sueharu , Nishiyama, Makoto , Yu, Fujio , Hashimoto, Yoshihiro
IPC分类号： C12N15/74 , C12N15/70 , C12N15/55 , C12N1/21
CPC分类号： C12N15/74 , C12N9/78
摘要： The present invention provides a recombinant plasmid comprising combining a hybrid plasmid vector with the isolated DNA sequences of one or more genes encoding nitrile degrading enzymes which are derived from bacteria belonging to the genus Rhodococcus,
said hybrid plasmid vector comprising
an isolated DNA sequence which confers the vector the ability to replicate and amplify in the cells of bacteria belonging to the genus Rhodococcus , and
an isolated DNA sequence which confers the vector the ability to replicate and amplify in the cells of bacteria belonging to Escherichia coli , and
an isolated DNA sequence containing a drug resistance gene.

5. 发明公开

EP0502476A2 Hybrid plasmid vectors containing genes encoding nitrile degrading enzymes and methods of producing amides and acids 失效
标题翻译：含有编码酶的基因Nitrilabbauende杂种质粒载体和使用酰胺和酸的制备方法。
公开(公告)号：EP0502476A2
公开(公告)日：1992-09-09
申请号：EP92103610.9
申请日：1992-03-03
申请人： NITTO CHEMICAL INDUSTRY CO., LTD. , Beppu, Teruhiko
发明人： Beppu, Teruhiko , Horinouchi, Sueharu , Nishiyama, Makoto , Yu, Fujio , Hashimoto, Yoshihiro
IPC分类号： C12N15/74 , C12N15/70 , C12N15/55 , C12N1/21
CPC分类号： C12N15/74 , C12N9/78
摘要： The present invention provides a recombinant plasmid comprising combining a hybrid plasmid vector with the isolated DNA sequences of one or more genes encoding nitrile degrading enzymes which are derived from bacteria belonging to the genus Rhodococcus,
said hybrid plasmid vector comprising
an isolated DNA sequence which confers the vector the ability to replicate and amplify in the cells of bacteria belonging to the genus Rhodococcus , and
an isolated DNA sequence which confers the vector the ability to replicate and amplify in the cells of bacteria belonging to Escherichia coli , and
an isolated DNA sequence containing a drug resistance gene.
摘要翻译：本发明提供了一种重组质粒，其包括混合型质粒载体与来自属于红球菌属的细菌衍生的哪个的编码腈降解酶的一种或多种基因的分离的DNA序列结合，所述包括分离的DNA序列的杂种质粒载体，其赋予所述向量复制和在属于红球菌属细菌的细胞中扩增，并赋予该载体的复制和扩增在属于埃希氏杆菌细菌的细胞的能力的分离的DNA序列，以及含有一个分离的DNA序列的能力药物抗性基因。

6. 发明公开

EP0073029A3 Recombinant plasmid and microorganism containing same 有权
标题翻译：重组质粒和含有其的微生物
公开(公告)号：EP0073029A3
公开(公告)日：1983-06-29
申请号：EP82107601
申请日：1982-08-19
申请人： Beppu, Teruhiko
发明人： Beppu, Teruhiko , Uozumi, Takeshi , Nishimori, Katsuhiko
IPC分类号： C12N15/00 , C12N09/50 , C12N01/20 , C12R01/19
CPC分类号： C12N9/6481 , C12N9/52
摘要： Messenger RNA of prorennin is extracted from the fourth stomach of calf and the double-stranded prorennin cDNA is prepared therefrom. The prorennin cDNA is cloned into a host microorganism, E. coli C600r k m k , to give clones containing the cDNA insert with variable size as confirmed by the base sequence determination in addition to the colony hybridization and hybrid-arrested translation technique. In vitro recombination of two plasmids obtainable from these clones, pTACR102B6 and pTACR103C2, affords plasmid pCR1001 containing the whole coding sequence for prorennin. On addition of the lac operon of E. coli to pCR1001, plasmid pCR2001 is prepared, which plasmid contains the prorennin cDNA sequence and the promoter requisite for the expression of the prorennin gene in a host cell. Transformation of E. coli C600r k m k by inserting pCR2001 produces a new E. coli strain, E. coli CR1 fully designated as E. coli CR1(r k m k thr , leu , thi , pCR2001). This genetically engineered new strain of E. coli, when propagated in aqueous nutrient media, produces prorennin. Thus production by bacteria of prorennin which is a proenzyme and a precursor of the milk coagulating enzyme, calf rennin, has now been accomplished.

7. 发明公开

EP0154351A2 Expression plasmids useful in B. subtilis 有权
标题翻译：在枯草芽孢杆菌verwendbare表达质粒。
公开(公告)号：EP0154351A2
公开(公告)日：1985-09-11
申请号：EP85102659.1
申请日：1985-03-08
申请人： Beppu, Teruhiko
发明人： Beppu, Teruhiko , Uozumi, Takeshi , Tsuchiya, Makoto , Sekine, Susumu
IPC分类号： C12N15/59 , C12N15/62 , C12N15/75
CPC分类号： C12N9/54 , C07K2319/00 , C12N9/6481 , C12N15/75
摘要： A new vector system is described for expression ot prochymosin in B. subtilis. The vector system includes the prochymosin encoding gene and an erythromycin resistant promotor and can be integrated into a B. subtilis host organism as an expression plasmid adapted for transformation.
摘要翻译：描述了用于在枯草芽孢杆菌中表达凝乳酶原的新载体系统。载体系统包括凝乳酶原编码基因和红霉素抗性启动子，并且可以作为适于转化的表达质粒整合到枯草芽孢杆菌宿主生物体中。

8. 发明公开

EP0121775A1 Novel expression plasmids and their use in the method for expressing prochymosin coding gene in E. coli 失效
标题翻译：在大肠杆菌中表达质粒和我的Verwendung im Verfahren zur表达von Prochymosin codierendem Gen。
公开(公告)号：EP0121775A1
公开(公告)日：1984-10-17
申请号：EP84102451.6
申请日：1984-03-07
申请人： Beppu, Teruhiko
发明人： Beppu, Teruhiko , Uozumi, Takeshi , Nishimori, Katsuhiko , Shimizu, Norio , Kawaguchi, Yoshiyuki , Hidaka, Makoto
IPC分类号： C12N15/00 , C12N9/50
CPC分类号： C12N9/6481 , C07K1/1136 , C07K2319/00 , C12N9/6483 , C12N15/71 , C12Y304/23004
摘要： improved methods are provided for replication and expression of prochymosin coding gene in E. coli. Novel expression plasmids having prochymosin coding gene operatively attached to the E. coli trp operon have been developed. These plasmids have been inserted into E. coli host cells by transformation, providing new strains to E. coli with high expression capability. The techniques for preparing such expression plasmids and E. coli strains derived therefrom are described. The modified cells, under appropriate cultivation conditions, produces a substantial amount of prochymosin which on activation (viz., in the form of chymosin) displays milk-clotting activity. The genetically engineered new strains of E. coli have been deposited with the Fermentation Research Institute, lbaragi, Japan under the Budapest Treaty. The methods are thus useful in the massive production of prochymosin by fermentation, and are superior to the known art in the field.
摘要翻译：提供改进的方法用于在大肠杆菌中复制和表达凝乳酶原编码基因。已经开发了具有可操作地连接到大肠杆菌trp操纵子的凝乳酶原编码基因的新型表达质粒。通过转化将这些质粒插入到大肠杆菌宿主细胞中，向具有高表达能力的大肠杆菌提供新的菌株。描述了制备这样的表达质粒和从其衍生的大肠杆菌菌株的技术。在适当的培养条件下，修饰的细胞产生大量的凝乳酶原，其在激活时（即以凝乳酶的形式）显示出乳凝血活性。根据布达佩斯条约，基因工程新菌株E. coil已经保藏在日本伊藤堡发酵研究所。因此，这些方法可用于通过发酵大规模生产凝乳酶原，并且优于本领域已知的技术。

9. 发明公开

EP0073029A2 Recombinant plasmid and microorganism containing same 有权
标题翻译： Rekombinantes Plasmid und dasselbe enthaltender Mikroorganismus。
公开(公告)号：EP0073029A2
公开(公告)日：1983-03-02
申请号：EP82107601.5
申请日：1982-08-19
申请人： Beppu, Teruhiko
发明人： Beppu, Teruhiko , Uozumi, Takeshi , Nishimori, Katsuhiko
IPC分类号： C12N15/00 , C12N9/50 , C12N1/20
CPC分类号： C12N9/6481 , C12N9/52
摘要： Messenger RNA of prorennin is extracted from the fourth stomach of calf and the double-stranded prorennin cDNA is prepared therefrom. The prorennin cDNA is cloned into a host microorganism, E. coli C600r k m k , to give clones containing the cDNA insert with variable size as confirmed by the base sequence determination in addition to the colony hybridization and hybrid-arrested translation technique. In vitro recombination of two plasmids obtainable from these clones, pTACR102B6 and pTACR103C2, affords plasmid pCR1001 containing the whole coding sequence for prorennin. On addition of the lac operon of E. coli to pCR1001, plasmid pCR2001 is prepared, which plasmid contains the prorennin cDNA sequence and the promoter requisite for the expression of the prorennin gene in a host cell. Transformation of E. coli C600r k m k by inserting pCR2001 produces a new E. coli strain, E. coli CR1 fully designated as E. coli CR1(r k m k thr , leu , thi , pCR2001). This genetically engineered new strain of E. coli, when propagated in aqueous nutrient media, produces prorennin. Thus production by bacteria of prorennin which is a proenzyme and a precursor of the milk coagulating enzyme, calf rennin, has now been accomplished.
摘要翻译：从小牛的第四胃提取肾上腺素的信使RNA，并制备双链肾上腺素cDNA。将prorennin cDNA克隆到宿主微生物大肠杆菌C600r @ m @中，以得到含有可变大小的cDNA插入片段的克隆，除了克隆杂交和杂交捕获翻译技术之外，通过碱基序列测定证实。从这些克隆获得的两个质粒pTACR102B6和pTACR103C2的体外重组提供了含有prorennin整个编码序列的质粒pCR1001。在将大肠杆菌的lac操纵子添加到pCR1001中后，制备质粒pCR2001，该质粒含有肾上腺素cDNA序列和在宿主细胞中表达prorennin基因所需的启动子。通过插入pCR2001转化大肠杆菌C600r @ m @产生新的大肠杆菌菌株，大肠杆菌CR1完全指定为大肠杆菌CR1（r @ m @，thr，leu，thi- ，pCR2001）。当在含水营养培养基中繁殖时，这种基因工程的大肠杆菌新菌株产生丙氨宁。因此，现在已经实现了作为乳酸酶和乳凝固酶，牛犊素前体的前列腺素细菌的生产。

10. 发明公开

EP0015552A3 Process for producing sphingomyelinase, sphingomyelinase and the use thereof 有权
标题翻译：生产丝氨酸蛋白酶，丝氨酸激酶及其用途的方法
公开(公告)号：EP0015552A3
公开(公告)日：1980-10-15
申请号：EP80101123
申请日：1980-03-06
申请人： Chiyoda Chemical Engineering & Construction Company Limited , Beppu, Teruhiko
发明人： Beppu, Teruhiko , Ando, Noboru
IPC分类号： C12N09/16 , C12Q01/44
CPC分类号： C12Q1/44 , C12N9/16 , C12R1/38 , G01N2405/08 , Y10S435/874
摘要： A process for producing sphingomyelinase comprising cultivating a sphingomyelinase-producing microorganism, belonging to the genus Pseudomonas, in a culture medium and recovering sphingomyelinase from the culture medium, sphingomyelinase obtainable by such process and the use thereof in the fractionation determination of phospolipids.
摘要翻译：一种生产鞘磷脂酶的方法，包括在培养基中培养属于假单胞菌属的产生鞘磷脂酶的微生物，并从培养基中回收鞘磷脂酶。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式