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    • 61. 发明公开
    • Methods for predicting pregnancy outcome in a subject by hCG assay
    • Verfahren zur Prognose des Schwangerschaftsausganges bei einer mit einem HCG-Test untersuchten Person
    • EP1939624A1
    • 2008-07-02
    • EP08000563.0
    • 1999-02-03
    • The Trustees of Columbia University in the City of New York
    • O'Connor, John F.Kovalevskaya, Galina I.Birken, Steven
    • G01N33/53
    • G01N33/76C07K14/59C07K16/26G01N33/57434G01N33/57449G01N2333/59Y10S435/971Y10S436/811Y10S436/814Y10S436/818
    • A method for predicting pregnancy outcome in a subject by determing the amount of EPMI-hCG in a sample from the subject using B152 antibody produced by the hybridoma cell line deposited with the American Type Culture Collection under Designation No. HB-12467; and a second, labelled antibody which binds to the complex formed by B152 antibody and EPMI-hCG to determine the amount of EPMI-hCG in the sample; using a third antibody which binds to intact, non-nicked hCG; and a fourth, labelled antibody which binds to intact non-nicked hCG simultaneously with the third antibody to determine the amount of intact hCG in the sample; and comparing the amount of EPMI-hCG in the sample with either the amount of EPMI-hCG determined in a sample from a temporally matched, normal pregnant subject or a non-pregnant subject, wherein an amount of EPMI-hCG in the subject's sample similar to the amount of EPMI-hCG in the sample from the temporally matched, normal pregnant subject or non-pregnant subject respectively indicates a positive or negative outcome of the pregnancy.
    • 一种用于通过使用保藏在名称号为HB-12467的美国典型培养物保藏中心的杂交瘤细胞系产生的B152抗体来测定受试者样品中EPMI-hCG的量来预测受试者的妊娠结局的方法; 和第二个标记的抗体,其结合由B152抗体和EPMI-hCG形成的复合物,以确定样品中EPMI-hCG的量; 使用结合完整的非缺口hCG的第三抗体; 和第四个标记的抗体,其与第三抗体同时与完整的非切口hCG结合以确定样品中完整的hCG的量; 将样品中的EPMI-hCG的量与来自时间匹配的正常怀孕受试者或非怀孕受试者的样品中测定的EPMI-hCG的量进行比较,其中受试者样品中的EPMI-hCG的量相似 来自临时匹配的正常怀孕受试者或非怀孕对象的样品中EPMI-hCG的量分别表示怀孕的阳性或阴性结果。