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51. 发明公开

EP0902834A1 STABLE AVIDIN COMPOSITION AND METHODS USING SAME 失效
标题翻译：稳定AVIDINZUSAMMENSETZUNG和申请流程。IRER
公开(公告)号：EP0902834A1
公开(公告)日：1999-03-24
申请号：EP96937780.0
申请日：1996-10-28
申请人： BRISTOL-MYERS SQUIBB COMPANY
发明人： BURTON, Steven, J. , PEARSON, James, C. , EDWARDSON, Peter, A., D.
IPC分类号： C12N11 , A61L2 , A61L24 , C07K1 , C07K14 , C07K17 , C12P21
CPC分类号： C07K14/36 , A61L2/0035 , A61L24/106 , C07K1/22 , C07K14/465 , C07K14/75 , C07K17/10 , C12P21/06 , Y10S435/966 , Y10S435/967 , Y10S435/972 , Y10S530/81 , Y10S530/812 , Y10S530/813
摘要： Compositions and methods for avidin immobilized on an inert support material, e.g., agarose, are disclosed. The compositions have high activity levels of avidin and may further include a bulking agent, e.g., maltose, and a protectant to maintain the stability and integrity of the avidin agarose during lyophilization and terminal sterilization processes. These compositions have applicability in any instance wherein avidin, agarose and/or the avidin/biotin technology are useful. In particular, the present compositions are useful in an enzyme capture system to prepare fibrin monomer useful for fibrin sealants.

52. 发明授权

EP0632133B1 HIGHLY SENSITIVE DETERMINATION OF D-3-HYDROXYBUTYRIC ACID OR ACETOACETIC ACID AND COMPOSITION THEREFOR 有权
标题翻译： D-3-HYDROXYBUTIERSÄURE或乙酰及构成THEREFOR高敏感度测定
公开(公告)号：EP0632133B1
公开(公告)日：1998-04-22
申请号：EP92900594.0
申请日：1991-12-12
申请人： Asahi Kasei Kogyo Kabushiki Kaisha
发明人： UEDA, Shigeru , MISAKI, Hideo , IKUTA, Shigeru , TAKAHASHI, Mamoru
IPC分类号： C12Q1/32
CPC分类号： C12Q1/32 , Y10S435/966 , Y10S435/973 , Y10T436/104998

53. 发明公开

EP0645459A1 A method and apparatus for determining the role of cytochrome P450 and related enzymes in the metabolism of drugs and other chemicals 失效
标题翻译：一种用于确定药物和其他化学品的代谢细胞色素P450和相关酶的作用的方法和装置。
公开(公告)号：EP0645459A1
公开(公告)日：1995-03-29
申请号：EP94115144.1
申请日：1994-09-26
申请人： Cook, Dorn C. , Parkinson, Andrew
发明人： Cook, Dorn C. , Parkinson, Andrew
IPC分类号： C12Q1/00 , C12Q1/26 , C12Q1/32
CPC分类号： C12Q1/32 , C12Q1/26 , G01N2333/795 , G01N2333/80 , G01N2333/90245 , G01N2500/00 , Y10S435/966 , Y10S530/846
摘要： A method and apparatus for determining the enzyme or enzymes in the human body which metabolize a particular drug. Microsomes are obtained from each of several donors. The microsome for one donor is reacted with a test drug and the quantity of metabolites produced is determined. The microsome are similarly each reacted with the test drug and the quantity of metabolites produced for each microsome is determined to generate drug metabolism data. The drug metabolism data obtained is compared to reference data which indicates the activity of a select number of major enzymes in each of the donors. The reference data for each enzyme is separately tabulated. The enzyme responsible for the metabolism of the test drug is identified when the metabolism data correlates with the tabulated reference data for that enzyme.
摘要翻译：一种用于确定性采矿的一种或多种酶在人体内代谢哪一种药物的特殊方法和装置。微萨姆从每个若干捐助者获得的。对于一个供体的微粒体进行反应用测试药物和代谢物的产生的量是确定的开采。微粒体被类似地反应各用试验药物和每个微粒产生的代谢物的量是确定的开采，以产生药物代谢数据。所得药物的代谢数据进行比较，这表明一些选定在每个捐助者的主要酶的活性参考数据。对于每种酶的基准数据被单独列出。负责测试药物的代谢酶被识别当代谢数据与该酶的表格基准数据相关。

54. 发明授权

EP0422699B1 Methods for binding a compound on bibulous material 有权转让
标题翻译：一种用于将化合物与吸收性材料的结合过程。
公开(公告)号：EP0422699B1
公开(公告)日：1994-07-13
申请号：EP90124407.9
申请日：1986-02-13
申请人： ABBOTT LABORATORIES
发明人： Calderhead, David , Khanna, Pyare , Ullman, Edwin Fisher , Weng, Litai
IPC分类号： G01N33/546 , G01N33/549 , G01N33/548 , G01N33/558 , G01N33/52 , G01N33/543
CPC分类号： G01N33/54313 , G01N33/538 , G01N33/542 , G01N33/54386 , G01N33/558 , Y10S435/805 , Y10S435/966 , Y10S435/97 , Y10S435/973 , Y10S435/975 , Y10S436/81 , Y10S436/814 , Y10S436/818

55. 发明公开

EP0580070A2 Dry analytical element for acetaminophen assay 失效
标题翻译： Trockenes分析元素Fce死亡Aceminophen-Bestimmung。
公开(公告)号：EP0580070A2
公开(公告)日：1994-01-26
申请号：EP93111289.0
申请日：1993-07-14
申请人： Johnson & Johnson Clinical Diagnostics, Inc.
发明人： Schaeffer, James Robert , Mauck, John Charles, c/o Eastman Kodak Comp. , Winterkorn, Robert Francis , Arter, Thomas Charles
IPC分类号： C12Q1/34 , C12Q1/26
CPC分类号： C12Q1/34 , G01N2333/98 , Y10S435/966 , Y10S435/969 , Y10S435/97 , Y10S436/815
摘要： A spectrophotometric assay for the detection of acetaminophen in aqueous fluids can be carried out with a dry analytical element. The element comprises a support having thereon one or more reagent layers containing a first enzyme, aryl acylamidase, to cleave the amide bond of acetaminophen to produce p-aminophenol; a second enzyme, ascorbic acid oxidase, to oxidize the p-aminophenol so that it couples to a water soluble coupling agent to form a dye that is read at 670nm. The assay is precise, accurate on serum and plasma samples, and relatively free from significant interferences. The element also allows measurement over a broad dynamic range.
摘要翻译：用于检测水性流体中对乙酰氨基酚的分光光度测定法可以用干燥的分析元件进行。元件包括其上具有一个或多个含有第一种酶的试剂层的载体，芳基酰胺酶，以切割对乙酰氨基酚的酰胺键以产生对氨基苯酚; 第二种酶，抗坏血酸氧化酶，以氧化对氨基苯酚，使其与水溶性偶联剂偶联形成在670nm读取的染料。该测定在血清和血浆样品上精确，准确，并且相对没有显着的干扰。该元件还允许在宽动态范围内进行测量。

56. 发明公开

EP0571939A1 Verfahren und Mittel zur Bestimmung eines Analyts 失效
标题翻译： Verfahren und Mittel zur Bestimmung eines分析。
公开(公告)号：EP0571939A1
公开(公告)日：1993-12-01
申请号：EP93108403.2
申请日：1993-05-25
申请人： BOEHRINGER MANNHEIM GMBH
发明人： Pollmann, Klaus, Dr. , Freitag, Helmut, Dr. , Rothe, Anselm, Dr.
IPC分类号： C12Q1/34 , G01N33/58
CPC分类号： C12Q1/34 , G01N33/581 , G01N2333/924 , G01N2400/22 , Y10S435/962 , Y10S435/966 , Y10S435/969 , Y10S435/97
摘要： Gegenstand der Erfindung ist ein neues Verfahren zum Nachweis einer Substanz mit Hydrolaseaktivität in einer Probe, dadurch gekennzeichnet, daß man die Probe mit einem an ein unlösliches Trägermaterial kovalent gebundenen Indikatorenzym, das durch die Hydrolase-Aktivität löslich gemacht werden kann, in Kontakt bringt, man das abgespaltene Indikatorenzym abtrennt und seine enzymatische Aktivität bestimmt und ein Mittel zu seiner Ausführung.
摘要翻译：本发明涉及一种用于检测样品中具有水解酶活性的物质的方法，其特征在于，所述样品与共价键合到不溶性载体材料并可通过水解酶活性变得可溶的指示剂酶接触，所述指示剂已经被切除的酶被分离出并且其酶活性被确定，并且用于进行它的手段。

57. 发明授权

EP0156641B1 Enzymic method of detecting analytes and novel substrates therefor 有权转让
标题翻译：检测分析物和新型底物的酶的方法
公开(公告)号：EP0156641B1
公开(公告)日：1992-06-10
申请号：EP85302045.1
申请日：1985-03-25
申请人： London Biotechnology Limited
发明人： Rabin, Brian Robert , Taylorson, Christopher John , Hollaway, Michael Robert
IPC分类号： G01N33/58 , G01N33/53 , C12Q1/34
CPC分类号： C12Q1/6816 , C07H21/00 , C12Q1/6823 , G01N33/535 , G01N33/542 , G01N33/581 , Y10S435/81 , Y10S435/966 , Y10S435/975

58. 发明公开

EP0451848A2 Immunoassay element and process for immunoassay 失效
标题翻译： Immuntestelement und VerfahrenfürImmuntest。
公开(公告)号：EP0451848A2
公开(公告)日：1991-10-16
申请号：EP91105795.8
申请日：1991-04-11
申请人： FUJI PHOTO FILM CO., LTD. , FUJIREBIO KABUSHIKI KAISHA also trading as FUJIREBIO INC.
发明人： Toshikage, Hiraoka, c/o Fuji Photo Film Co., Ltd. , Tanimoto, Tetsuji, c/o Fujirebio Inc. , Makino, Yoshihiko, c/o Fuji Photo Film Co., Ltd. , Ninomiya, Tadashi, c/o Fujirebio Inc. , Hora, Naofumi, c/o Fuji Photo Film Co., Ltd. , Ashihara, Yoshihiro, c/o Fujirebio Inc. , Sudo, Yukio, c/o Fuji Photo Film Co., Ltd. , Mori, Toshihiro, c/o Fuji Photo Film Co., Ltd.
IPC分类号： G01N33/543
CPC分类号： G01N33/54386 , Y10S435/966 , Y10S435/969 , Y10S435/97 , Y10S436/807 , Y10S436/81
摘要： An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity caused by a reaction between the ligand, a linked product of the ligand and a high molecular weight compound and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the non-difussible material. Also provided is a process for quantitatively analyzing a ligand contained in a sample by the use of the immunoassay element.
摘要翻译：一种免疫测定元件，用于通过测定配体，配体与高分子量化合物的连接产物和酶标记的抗体之间的反应引起的酶活性的变化来定量分析配体。免疫测定元件包括含有在酶存在下形成可扩散材料的非扩散性底物的底物层和含有用于进一步碎裂非可弥散材料的碎裂酶的试剂层。还提供了通过使用免疫测定元件定量分析样品中所含的配体的方法。

59. 发明公开

EP0422699A3 Methods for binding a compound on bibulous material 有权转让
标题翻译：在BIBULOUS材料上混合化合物的方法
公开(公告)号：EP0422699A3
公开(公告)日：1991-05-08
申请号：EP90124407.9
申请日：1986-02-13
申请人： ABBOTT LABORATORIES
发明人： Calderhead, David , Khanna, Pyare , Ullman, Edwin Fisher , Weng, Litai
IPC分类号： G01N33/546 , G01N33/549 , G01N33/548 , G01N33/558 , G01N33/52 , G01N33/543
CPC分类号： G01N33/54313 , G01N33/538 , G01N33/542 , G01N33/54386 , G01N33/558 , Y10S435/805 , Y10S435/966 , Y10S435/97 , Y10S435/973 , Y10S435/975 , Y10S436/81 , Y10S436/814 , Y10S436/818
摘要： A method for non-diffusively binding a member of a specific binding pair ("sbp" member) on bibulous material. The method comprises applying in a pattern on the bibulous material, particles to which the sbp members are non-diffusively bound. The size of the particles are sufficiently small to allow them to infiltrate the pores of the bibulous material. Their size is also sufficiently large to prevent them from diffusing from the pores.

60. 发明公开

EP0243797A3 Method for amplifying enzyme activity, enzyme conjugates suitable therefor and their preparation 无效
标题翻译：放大酶活性的方法，适用于其的酶合成及其制备
公开(公告)号：EP0243797A3
公开(公告)日：1990-09-05
申请号：EP87105569.5
申请日：1987-04-15
申请人： NORTHEASTERN UNIVERSITY
发明人： Giese, Roger W. , Ehrat, Markus , Cecchini, Douglas J.
IPC分类号： C12Q1/00 , C12Q1/68
CPC分类号： C12Q1/68 , C12Q1/00 , Y10S435/962 , Y10S435/966 , C12Q2521/543 , C12Q2565/518
摘要： The invention relates to a method for amplifying enzyme activity, spcecific enzyme conjugates suitable therefor and a method for their preparation. Enzyme amplification is achieved by covalently bonding enzyme to a support material via a molecular chain which is a substrate for the enzyme, then introducing a small amount of free enzyme to this system, causing release of a large amount of bound enzyme. Alternatively, complementary enzymatically inactive fragments of an active enzyme, which can recombine to form active enzyme, are covalently attached to separate support materials by a molecular chain material which is a substrate for the active enzyme, and these two fragment-support conjugates are connected in series. In a second alternative embodiment, two different active enzymes may be used. The method is suited e.g. for the S-peptide and S-protein fragments of ribonuclease S (RNaseS) which are preferably coupled to Sepharose gels, via a polycytidylic acid substrate leash. Also the released fragments recombine to give RNaseS activity. Thus this system provides more enzymatic activity from the system than is applied. 20 hours to yield activity equivalent to 1.911-g of RNase, by applying it to the S-peptide gel followed by combination of the released S-peptide with excess S-protein. Also, application of 1 ng of RNaseA to a three-stage amplification system in which each stage consists of a pair of S-peptide and S-protein gels produced cumulative amplifications of 4, 9, 52 and 25x after each to the stages, respectively. Only 1 - 2 mg amounts of each gel are used per stage in these experiments, reflecting the significant production of RNaseS activity. Molecular amplification factors of -2.104 can be achieved.

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