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1. 发明公开

EP0884591A1 Immunoassay element 失效
标题翻译：免疫测定的元素
公开(公告)号：EP0884591A1
公开(公告)日：1998-12-16
申请号：EP98110905.1
申请日：1998-06-15
申请人： FUJI PHOTO FILM CO., LTD.
发明人： Sakaino, Yoshiki , Ito, Hitomi , Mori, Toshihiro , Seshimoto, Osamu , Ito, Toshihisa , Amano, Yoshikazu
IPC分类号： G01N33/00 , C12Q1/40 , C12Q1/54 , C12Q1/28
CPC分类号： G01N33/54386 , G01N2333/924 , Y10S435/966 , Y10S435/969 , Y10S435/97 , Y10S436/807 , Y10S436/81
摘要： The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the lebelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.
摘要翻译：用于通过确定由免疫反应引起的酶标记的抗原或抗体的酶活性的变化来定量分析抗原的免疫测定元件。免疫测定元件包括含有在标记酶存在下形成可扩散材料的非扩散性底物的底物层和含有用于将可扩散材料进一步碎裂成较低分子量产物的碎裂酶的试剂层。作为非扩散性底物，可以使用能够仅与前体酶反应并且不能使片段化酶反应的底物。当使用内源活性葡糖苷酶作为标记酶，并且在试剂层中使用断裂酶时，外源活性葡糖苷酶是底物层的非扩散性基质优选为内型选择性反应性底物，其中是指具有对内源性葡糖苷酶特异性的反应性的底物。高灵敏度测定以高精度，高重现性和良好的储存稳定性实现。

2. 发明授权

EP0571939B1 Mittel zur Bestimmung eines Analyts 失效
标题翻译：用于确定分析物
公开(公告)号：EP0571939B1
公开(公告)日：1998-09-09
申请号：EP93108403.2
申请日：1993-05-25
申请人： BOEHRINGER MANNHEIM GMBH
发明人： Pollmann, Klaus, Dr. , Freitag, Helmut, Dr. , Rothe, Anselm, Dr.
IPC分类号： C12Q1/34 , G01N33/58
CPC分类号： C12Q1/34 , G01N33/581 , G01N2333/924 , G01N2400/22 , Y10S435/962 , Y10S435/966 , Y10S435/969 , Y10S435/97

3. 发明授权

EP0440775B1 FACTOR IX CHROMOGENIC ASSAY 有权转让
标题翻译：因子IX显色试验。
公开(公告)号：EP0440775B1
公开(公告)日：1995-08-30
申请号：EP90913146.8
申请日：1990-08-16
申请人： Dade Produktions AG
发明人： HEMKER, Hendrik Coenraad
IPC分类号： C12Q1/56 , C12Q1/37 , G01N33/86
CPC分类号： C12Q1/56 , C12Q2337/12 , G01N2333/96444 , G01N2333/9645 , G01N2333/96452 , Y10S435/966 , Y10S435/975 , Y10S436/808
摘要： A chromogenic assay for determination of blood coagulation Factor IX (christmas factor) utilizes Factor Xa formed by the conversion of Factor X by activated Factor IX to cleave a chromogenic substrate. Conversion of Factor X to Factor Xa is coupled to a Factor XI mediated conversion to Factor IXa in a substantially two step procedure.

4. 发明公开

EP0632133A1 HIGHLY SENSITIVE DETERMINATION OF D-3-HYDROXYBUTYRIC ACID OR ACETOACETIC ACID AND COMPOSITION THEREFOR 有权
标题翻译：在ZUSAMMENSETZUNGDAFÜR上制造BESTIMMUNG VON D-3-HYDROXYBUTIERSÄUREODERACETESSIGSÄURE。
公开(公告)号：EP0632133A1
公开(公告)日：1995-01-04
申请号：EP92900594.0
申请日：1991-12-12
申请人： Asahi Kasei Kogyo Kabushiki Kaisha
发明人： UEDA, Shigeru , MISAKI, Hideo , IKUTA, Shigeru , TAKAHASHI, Mamoru
IPC分类号： C12Q1/32
CPC分类号： C12Q1/32 , Y10S435/966 , Y10S435/973 , Y10T436/104998
摘要： Determination of D-3-hydroxybutyric acid or acetoacetic acid by reacting a specimen solution with a reagent containing (1) D-3-hydroxybutyric acid dehydrogenase, (2) A₁, and (3) B₁ to form the cycle (I) and determining the amount of A₂ formed or the amount of B₁ consumed; and a composition containing the ingredients (1), (2), and (3) for use in the above determination method. The method and the composition make it possible to determine a small amount of a specimen solution conveniently with high sensitivity and are useful in the field of clinical inspection and food testing.
摘要翻译：通过使样品溶液与含有（1）D-3-羟基丁酸脱氢酶，（2）A1和（3）B1的试剂反应形成循环（I）并测定D-3-羟基丁酸形成的A2的量或消耗的B1的量; 和含有上述测定方法中使用的成分（1），（2）和（3）的组合物。该方法和组成使得可以方便地以高灵敏度确定少量的样品溶液，并且可用于临床检查和食品测试领域。

5. 发明公开

EP0526952A2 Soluble interleukin-2 receptor as a disease indicator and a method of assaying the same 失效
标题翻译：可溶性白细胞介素2受体作为指示剂疾病和方法其检测。
公开(公告)号：EP0526952A2
公开(公告)日：1993-02-10
申请号：EP92202916.0
申请日：1986-04-14
申请人： THE UNITED STATES OF AMERICA as represented by the Secretary United States Department of Commerce
发明人： Nelson, David L. , Biddison, William E. , Rubin, Laurence A. , Greene, Warner C. , Leonard, Warren J. , Yauchoan, Robert
IPC分类号： G01N33/68 , C12P21/02 , G01N33/543 , G01N33/566 , G01N33/577
CPC分类号： G01N33/6869 , C07K16/2866 , Y10S435/81 , Y10S435/966 , Y10S435/967 , Y10S435/969 , Y10S435/975 , Y10S436/80 , Y10S436/808 , Y10S436/809 , Y10S436/813 , Y10S530/868
摘要： The present invention discloses a sandwich method and a kit for assaying interleukin-2 receptor (IL-2R) in a sample. The method comprises determining reactivity of said sample with a plurality of ligands, each said ligand having binding affinity for a specific site on the receptor, said site being different for each said ligand and distinct from interleukin-2 binding site on the receptor. The invention also discloses a method of detecting such disturbed or abnormal conditions in humans which release soluble IL-2R in the body fluid.
摘要翻译：本发明盘松动夹心法和用于测定样品中白细胞介素-2受体（IL-2R）的试剂盒。该方法包括将所述样品的确定性采矿反应性配位体的复数，具有在受体上的特定位点的结合亲和性，每个所述配体，所述部位是从对受体白细胞介素-2结合位点对于每个所述配体不同且独特的。因此，本发明盘松动检测的求干扰或异常状况在人类其中在体液释放可溶性IL-2R的方法。

6. 发明授权

EP0143574B1 Assay method and kit for whole blood samples 有权转让
标题翻译：全血样品的测定方法和试剂盒
公开(公告)号：EP0143574B1
公开(公告)日：1989-01-18
申请号：EP84307633.2
申请日：1984-11-01
申请人： SYNTEX (U.S.A.) INC.
发明人： Zuk, Robert F.
IPC分类号： G01N33/543 , G01N33/52
CPC分类号： G01N33/80 , G01N33/54386 , Y10S435/805 , Y10S435/962 , Y10S435/966 , Y10S435/97 , Y10S435/975 , Y10S436/808 , Y10S436/81 , Y10S436/825 , Y10S436/827

7. 发明公开

EP0278116A2 A method for immunoassay and kit therefor 失效
标题翻译： Ein Verfahren zum Immuntest und Reagenzsatzdafür。
公开(公告)号：EP0278116A2
公开(公告)日：1988-08-17
申请号：EP87119257.1
申请日：1987-12-28
申请人： HITACHI, LTD.
发明人： Imai, Kyoko, c/o Makiguchi , Nomura, Yasushi
IPC分类号： G01N33/546 , G01N33/58 , G01N33/532 , G01N33/74
CPC分类号： G01N33/5432 , G01N33/74 , Y10S435/966 , Y10S435/975 , Y10S436/821 , Y10S436/829
摘要： Microcapsule-reagent are prepared by previously reacting at least a part of an antigen or antibody attached to microcapsules encapsulating a labeling substance with an antibody or antigen which is specifically reactive therewith, and then the reagent thus prepared is reacted with a sample containing an antigen or antibody in the presence of a complement, whereby highly sensitive immunoassay of the antigen or antibody in the sample can be realized even when the antigen or antibody attached to the microcapsules has a lowered activity or has only low reactivity with the antibody or antigen in the sample.
摘要翻译：微胶囊试剂通过预先使与包封标记物质的微胶囊连接的抗原或抗体的至少一部分与与其特异性反应的抗体或抗原反应，然后将如此制备的试剂与含有抗原或即使在附着于微胶囊的抗原或抗体具有降低的活性或仅与样品中的抗体或抗原的反应性低的情况下，也可以实现样品中抗原或抗体的高度灵敏的免疫测定。

8. 发明公开

EP0185738A4 IMMUNOLIPOSOME ASSAY - METHODS AND PRODUCTS. 失效
标题翻译：免疫脂质体测试方法和产品。
公开(公告)号：EP0185738A4
公开(公告)日：1988-07-14
申请号：EP85903151
申请日：1985-06-11
申请人： UNIV TENNESSEE RES CORP
发明人： HUANG LEAF , HO RODNEY JIN YONG
IPC分类号： G01N33/543 , G01N33/532 , G01N33/544 , G01N33/58
CPC分类号： G01N33/532 , G01N33/586 , Y10S435/966 , Y10S435/967 , Y10S435/975 , Y10S436/815 , Y10S436/829
摘要： A new membrane lytic immunoassay. In one embodiment of this assay, an antigen is first covalently coupled with lipids and this antigen-lipid complex is mixed with a hexagonal phase forming lipid to form bilayer liposome vesicles additionally containing a self-quenching fluorescent dye. When this antigen-containing liposome is brought into contact with a solid surface coated with antibody molecules, binding occurs between the antigen and the antibody, disrupting the liposome and releasing the dye. To assay a biological fluid for free antigen the fluid is first contacted with the solid surface-antibody complex, to saturate the bound antibody. Binding by the liposomes is thereby inhibited, leading to reduced dye release. Comparing dye release against a standardized curve of dye release versus known antigen concentrations allows for rapid determination of the unknown antigen concentration in the biological fluid. Similarly, antibodies and other entities, e.g., enzymes, drugs, etc., may be determined using slightly modified versions of this assay.

9. 发明公开

EP0245981A2 Signal enhancement in immunoassay by modulation of chemical catalysis 失效
标题翻译： Signalerhöhungin einem Immunoassay durch Modulation der chemischen Katalyse。
公开(公告)号：EP0245981A2
公开(公告)日：1987-11-19
申请号：EP87303661.0
申请日：1987-04-24
申请人： Becton Dickinson and Company
发明人： Perlman, Michael E. , Evans, Susan A.
IPC分类号： G01N33/542
CPC分类号： G01N33/542 , G01N33/581 , Y10S435/81 , Y10S435/966 , Y10S436/808 , Y10S436/821
摘要： A method for immunoassay for a ligand suspected to be present in a fluid includes use of an enzyme, a metal ion catalyst for an indicator reaction and a blocked modulator for the catalyst. Ligand present in the fluid binds to an antiligand. The resulting bound fraction activates the enzyme to unblock the modulator. The free modulator activates or inhibits the catalyst thereby modulating the rates of an indicator reaction between a substrate and a redox reagent. The presence or absence of the ligand in the fluid is indicated by a signal, such as a colour change or a rate of colour change, consequent to the indicator reaction. The invention also includes a kit of materials useful for performing the method of the invention.
摘要翻译：怀疑存在于流体中的配体的免疫测定方法包括使用酶，用于指示剂反应的金属离子催化剂和催化剂的封闭调节剂。存在于流体中的配体与抗配体结合。所得到的结合部分激活酶以阻断调节剂。游离调节剂激活或抑制催化剂，从而调节底物和氧化还原剂之间的指示剂反应速率。流体中配体的存在或不存在由指示剂反应所引起的信号，例如颜色变化或颜色变化率。本发明还包括可用于实施本发明方法的一组材料。

10. 发明公开

EP0243797A2 Method for amplifying enzyme activity, enzyme conjugates suitable therefor and their preparation 无效
标题翻译：一种用于提高酶的活性的方法，化酶共轭体适合于该目的和它们的制备。
公开(公告)号：EP0243797A2
公开(公告)日：1987-11-04
申请号：EP87105569.5
申请日：1987-04-15
申请人： NORTHEASTERN UNIVERSITY
发明人： Giese, Roger W. , Ehrat, Markus , Cecchini, Douglas J.
IPC分类号： C12Q1/00 , C12Q1/68
CPC分类号： C12Q1/68 , C12Q1/00 , Y10S435/962 , Y10S435/966 , C12Q2521/543 , C12Q2565/518
摘要： The invention relates to a method for amplifying enzyme activity, spcecific enzyme conjugates suitable therefor and a method for their preparation.
Enzyme amplification is achieved by covalently bonding enzyme to a support material via a molecular chain which is a substrate for the enzyme, then introducing a small amount of free enzyme to this system, causing release of a large amount of bound enzyme. Alternatively, complementary enzymatically inactive fragments of an active enzyme, which can recombine to form active enzyme, are covalently attached to separate support materials by a molecular chain material which is a substrate for the active enzyme, and these two fragment-support conjugates are connected in series. In a second alternative embodiment, two different active enzymes may be used. The method is suited e.g. for the S-peptide and S-protein fragments of ribonuclease S (RNaseS) which are preferably coupled to Sepharose gels, via a polycytidylic acid substrate leash. Also the released fragments recombine to give RNaseS activity. Thus this system provides more enzymatic activity from the system than is applied. 20 hours to yield activity equivalent to 1.911-g of RNase, by applying it to the S-peptide gel followed by combination of the released S-peptide with excess S-protein. Also, application of 1 ng of RNaseA to a three-stage amplification system in which each stage consists of a pair of S-peptide and S-protein gels produced cumulative amplifications of 4, 9, 52 and 25x after each to the stages, respectively. Only 1 - 2 mg amounts of each gel are used per stage in these experiments, reflecting the significant production of RNaseS activity.
Molecular amplification factors of -2.104 can be achieved.
摘要翻译：本发明涉及的方法用于扩增酶活性，spcecific酶偶联物适合于此的方法和用于它们的制备。酶扩增通过共价到载体材料经由一个分子链中的所有其是酶的底物结合的酶，然后引入游离酶少量该系统，引起大量的结合的酶的释放来实现。可替代地，活性酶，其可重新结合形成活性酶的互补无酶活性的片段共价由分子链材料的所有其对于活性酶的底物附着到单独的载体材料，和论文2片段 - 载体缀合物被连接在系列。在第二替代实施例中，可以使用两种不同的活性酶。该方法是适合E.G. 用于S-肽和核糖核酸酶S-（RNA酶），其优选地耦合到琼脂糖凝胶的S-蛋白质片段，通过胞苷酸基板皮带。因此，释放的片段重组给予核糖核酸酶和活性。因此，该系统提供了从系统比应用更酶活性。 20小时，以得到同等活性1.9亩克RNA酶的，通过将其应用到随后用过量S-蛋白释放的S-肽的组合的S-肽凝胶。所以，分别应用1纳克的RNase A的，其中一对S-肽和S-蛋白凝胶中的每一级besteht产生的4，图9，52和25倍累积扩增每个所述阶段之后，一个三阶段的放大系统，每个凝胶的只有1〜2毫克量以每级中使用在合成实验，反映了显著生产RNA酶和活性。的相似2.10分子扩增因子<4>可以实现的。

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