专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

11. 发明公开

EP2481287A1 Human skin equivalents expressing exogenous polypeptides 有权
标题翻译：外源多肽活性成分
公开(公告)号：EP2481287A1
公开(公告)日：2012-08-01
申请号：EP12162268.2
申请日：2004-07-30
申请人： Stratatech Corporation
发明人： Centanni, John M. , Allen-Hoffmann, Lynn
IPC分类号： A01N63/00 , C12N5/00 , C12N5/07 , C12N5/071 , C12N15/00 , C12N15/63
CPC分类号： A61L27/60 , A61K35/12 , A61K35/36 , A61K38/1709 , A61K38/1825 , A61K2035/122 , A61L27/227 , A61L27/3813 , A61L27/3895 , A61L27/54 , A61L2300/252 , A61L2300/404 , C07H21/04 , C07K14/50 , C12N5/0629 , C12N5/0698 , C12N15/63 , C12N15/79 , C12N2501/117 , C12N2501/998 , C12N2502/094 , C12N2502/1323 , C12N2510/00
摘要： The present invention relates generally to compositions for wound closure. More specifically, the present invention provides human skin equivalents engineered to express exogenous polypeptides (e.g., antimicrobial polypeptides and keratinocyte growth factor 2) and compositions and methods for making human skin equivalents engineered to express exogenous polypeptides. In addition, the present invention provides methods for treatment of wounds with human skin equivalents engineered to express exogenous polypeptides.
摘要翻译：本发明一般涉及用于伤口闭合的组合物。更具体地，本发明提供了工程化以表达外源多肽（例如抗微生物多肽和角质形成细胞生长因子2）的人皮肤当量，以及用于制备人皮肤等同物的工程化以表达外源多肽的组合物和方法。此外，本发明提供了用于表达外源多肽的用于治疗具有人皮肤当量的伤口的伤口的方法。

12. 发明公开

EP2377403A1 A genetically modified dermal micro-organ expressing factor VIII 审中-公开
标题翻译： Genetisch modifizierter dermisches mikroorgan-ausdrückenderFaktor VIII
公开(公告)号：EP2377403A1
公开(公告)日：2011-10-19
申请号：EP11174257.3
申请日：2004-04-29
申请人： Medgenics, Inc.
发明人： Bellomo, Stephen, F. , Lippin, Itzhak , Piva, Guillermo, Alberto , Rosenberg, Lior , Bukhman, Mordechay , Stern, Baruch, S. , Shalhevet, David , Shavitt, Menachem, D. , Pearlman, Andrew, L. , Noam, Shani , Almon, Einat
IPC分类号： A01N63/00 , A01N65/00 , C12N5/00 , C12N5/02 , C12N5/071
CPC分类号： A61B17/3468 , A61B10/0275 , A61B17/32053 , A61B17/322 , A61B2017/0023 , A61B2017/00969 , A61B2017/2808 , A61B2017/320052 , A61B2017/320064 , A61B2017/3225 , A61B2217/005 , A61F2/105 , A61K35/36 , A61K38/1816 , A61K38/193 , A61K38/2066 , A61K38/215 , A61K38/27 , A61K38/28 , A61M5/425 , C12N5/0698 , C12N2502/094 , C12N2502/1323 , C12N2510/00 , Y10T83/929
摘要： The present invention is directed to a genetically modified dermal micro-organ expressing at least one recombinant gene product, wherein said dermal micro-organ consists essentially of a plurality of dermal components and does not include epidermal layers, which maintains the micro-architecture and three dimensional structure of the dermal tissue from which it is derived, having dimensions selected so as to enable passive diffusion of adequate nutrients and gases to cells of said dermal micro-organ and diffusion of cellular waste out of said cells so as to minimize cellular toxicity and concomitant death due to insufficient nutrition and accumulation of waste in said dermal micro organ, wherein at least some of said cells of said genetically modified dermal micro-organ express at least a portion of at least one recombinant gene product, wherein said recombinant gene product is factor VIII.
摘要翻译：本发明涉及表达至少一种重组基因产物的遗传修饰的真皮微器官，其中所述皮肤微器官基本上由多个皮肤成分组成，并且不包括保持微结构和三维结构的表皮层具有选定尺寸的真皮组织的三维结构，使得足够的营养物和气体能够被释放到所述皮肤微器官的细胞中并将细胞废物从所述细胞扩散，以使细胞毒性最小化由于所述真皮微器官中的营养不足和废物积聚引起的伴随死亡，其中所述经遗传修饰的真皮微器官的至少一些所述细胞表达至少一部分至少一种重组基因产物，其中所述重组基因产物是因子VIII。

13. 发明授权

EP1312669B1 CULTURED EPIDERMAL CELL SHEET, LAMINATED CULTURED SKIN SHEET AND PROCESS FOR PRODUCING THE SAME 有权
标题翻译：培养表皮细胞层层叠培养的皮肤层和方法及其
公开(公告)号：EP1312669B1
公开(公告)日：2011-09-14
申请号：EP01945763.9
申请日：2001-07-02
申请人： CellSeed Inc.
发明人： OKANO, Teruo , YAMATO, Masayuki , UTSUMI, Mika , KUSHIDA, Ai , KONNO, Chie , KIKUCHI, Akihiko
IPC分类号： C12N5/07 , C12N5/071
CPC分类号： A61L27/3804 , A61L27/362 , A61L27/60 , C12N5/0698 , C12N2502/094 , C12N2502/1323 , C12N2539/10
摘要： A cultured epidermal cell sheet or a laminated cultured skin sheet is produced by culturing cells on a cell culture support which comprises a base material coated with a temperature-responsive polymer having an upper or lower critical temperature of dissolution in water of from 0 to 80?C, laminating the cultured cell layers if necessary, and then (1) adjusting the temperature of the liquid culture medium to a level higher than the upper critical dissolution temperature or lower than the lower critical dissolution temperature; (2) closely adhering the cultured epidermal cell sheet or the laminated skin sheet to a polymer film; and (3) peeling off as such together with the polymer film. Because of not decomposing E-cadherin or laminin 5 as in the case of treating with dispase and suffering from little structural defects, the cultured epidermal cell sheet and laminated cultured skin sheet thus obtained are strongly expected as being applicable to clinical operations such as skin transplantation.

14. 发明公开

EP2311469A3 Methods and compositions for tissue regeneration 审中-公开
标题翻译：方法和组合物用于组织再生
公开(公告)号：EP2311469A3
公开(公告)日：2011-06-08
申请号：EP10012560.8
申请日：2003-09-05
申请人： DFB Technology Holdings, LLC
发明人： Rolland, Eric , Hunziker, Thomas , Mis, Beatrice , Rinsch, Christopher
IPC分类号： A61K35/00 , A61K48/00 , C07K5/00 , C07K14/00 , C12N15/85 , C12N15/86 , A61K35/12 , A61K35/36 , A61K38/18 , A61K38/20 , A61P17/02
CPC分类号： A61K35/36 , A61K9/0014 , A61K9/10 , A61K9/19 , A61K9/7015 , A61K35/33 , A61K38/18 , A61K38/19 , A61K38/363 , A61K38/4833 , A61K41/0023 , A61K47/10 , A61K48/00 , A61L15/40 , A61L26/0057 , A61L27/3616 , A61L27/3633 , A61L27/3804 , A61L27/3813 , A61L27/3886 , C12N5/0656 , C12N5/0698 , C12N2502/094 , C12N2502/1323 , C12N2510/00 , C12N2510/04 , A61K2300/00
摘要： The present invention provides the use and composition of matter of angiogenic or other growth/cytokine factors expressed by mixtures of allogeneic human cell strains or lines of various types and stages of differentiation. Also provided are unencapsulated preparations (mixed with or applied to extracellular matrix material or synthetic biocompatible substances) for the purpose of temporary application to wounds or defects in the skin or other tissues for the restoration of blood supplying connective tissue to enable organ-specific cells to reestablish organ integrity as well as to inhibit excessive scar formation.

15. 发明公开

EP2302035A2 A method of producing a cultured skin device 有权
标题翻译：一种生产培养皮肤装置的方法
公开(公告)号：EP2302035A2
公开(公告)日：2011-03-30
申请号：EP10012359.5
申请日：2003-03-03
申请人： UNIVERSITY OF CINCINNATI , Shriners Hospitals for Children
发明人： Boyce, Steven T.
IPC分类号： C12N5/071
CPC分类号： A61F2/105 , A61L27/3804 , A61L27/3886 , A61L27/3895 , A61L27/60 , C12N5/0698 , C12N2502/094 , C12N2502/1323
摘要： An in-vitro skin evaluation device comprising
cultured dermal cells directly attached to a biocompatible reticulated acellular matrix;
the matrix prepared from a matrix-forming collagen-containing fluid that is cast, frozen, and dehydrated,
the dermal cells providing a cellular lamination layer with cultured epidermal cells directly inoculated and directly attached thereto which establishes a basement membrane between the epidermal cells and the dermal cells.
摘要翻译：一种体外皮肤评估装置，其包含直接附着于生物相容性网状无细胞基质的培养的真皮细胞; 由含有基质的含胶原液体浇铸，冷冻和脱水制备的基质，真皮细胞提供具有培养的表皮细胞的细胞层叠层，直接接种并直接附着于其上，这在表皮细胞和表皮细胞之间建立了基底膜真皮细胞。

16. 发明公开

EP2225361A1 BIOENGINEERED TISSUE CONSTRUCTS AND METHODS FOR PRODUCTION AND USE 审中-公开
标题翻译：生物技术构建的生产和使用的组织建造和方法
公开(公告)号：EP2225361A1
公开(公告)日：2010-09-08
申请号：EP08854614.8
申请日：2008-11-26
申请人： Organogenesis, Inc.
发明人： WANG, Xianyan , FARIA, Katherine, C.
IPC分类号： C12N5/00 , A61L27/60
CPC分类号： C12N5/0068 , A61L27/3633 , A61L27/3683 , A61L27/3804 , C12N5/0698 , C12N2500/25 , C12N2500/36 , C12N2500/38 , C12N2500/40 , C12N2500/50 , C12N2500/90 , C12N2500/92 , C12N2501/11 , C12N2501/148 , C12N2501/395 , C12N2502/094 , C12N2502/1323 , C12N2533/50 , C12N2533/92
摘要： Bioengineered constructs are formed from cultured cells induced to synthesize and secrete endogenously produced extracellular matrix components without the requirement of exogenous matrix components or network support or scaffold members. The bioengineered constructs of the invention can be treated in various ways such that the cells of the bioengineered constructs can be devitalized and/or removed without compromising the structural integrity of the constructs. Moreover, the bioengineered constructs of the invention can be used in conjunction with biocompatible/bioremodelable solutions that allow for various geometric configurations of the constructs.

17. 发明公开

EP1937326A4 ISOLATION AND CULTIVATION OF STEM/PROGENITOR CELLS FROM THE AMNIOTIC MEMBRANE OF UMBILICAL CORD AND USES OF CELLS DIFFERENTIATED THEREFROM 有权
标题翻译：分离和干/祖细胞的生长关闭羊膜帘线及其用途分化细胞
公开(公告)号：EP1937326A4
公开(公告)日：2010-08-11
申请号：EP06799864
申请日：2006-10-11
申请人： CELLRES CORP PTE LTD
发明人： PHAN TOAN-THANG
IPC分类号： A61L27/60 , A61K9/70 , A61K31/775 , A61P17/02 , C12M3/04 , C12N5/071 , C12N5/073 , C12N5/077
CPC分类号： C12N5/0655 , A61K35/32 , A61L27/18 , A61L27/3633 , A61L27/3804 , A61L27/3839 , A61L27/56 , A61L27/60 , C12N5/0605 , C12N5/0629 , C12N5/0668 , C12N5/0676 , C12N5/0698 , C12N2501/11 , C12N2501/115 , C12N2502/094 , C12N2502/1323 , C12N2506/02 , C12N2506/1369 , C12N2533/54 , C08L69/00
摘要： The present invention relates to a skin equivalent and a method for producing the same, wherein the skin equivalent comprises a scaffold and stem/progenitor cells isolated from the amniotic membrane of umbilical cord. These stem/progenitor cells may be mesenchymal (UCMC) and/or epithelial (UCEC) stem cells, which may then be further differentiated to fibroblast and keratinocytes. Further described is a method for isolating stem/progenitor cells from the amniotic membrane of umbilical cord, wherein the method comprises separating the amniotic membrane from the other components of the umbilical cord in vitro, culturing the amniotic membrane tissue under conditions allowing cell proliferation, and isolating the stem/progenitor cells from the tissue cultures. The invention also refers to therapeutic uses of these skin equivalents. Another aspect of the invention relates to the generation of a mucin-producing cell using stem/progenitor cells obtained from the amniotic membrane of umbilical cord and therapeutic uses of such mucin-producing cells. In yet another aspect, the invention relates to a method for generating an insulin-producing cell using stem/progenitor cells isolated from the amniotic membrane of umbilical cord and therapeutic uses thereof. The invention further refers to a method of treating a bone or cartilage disorder using UCMC. Furthermore, the invention refers to a method of generating a dopamin and tyrosin hydroxylase as well as a HLA-G and hepatocytes using UCMC and/or UCEC. The present invention also refers to a method of inducing proliferation of aged keratinocytes using UCMC.
摘要翻译：本发明涉及一种皮肤等同物和其制造，worin皮肤等同物的方法包括一个支架和茎从脐带的羊膜分离/祖细胞。这些干细胞/祖细胞可以是间充质（UCMC）和/或上皮细胞（UCEC）干细胞，其然后可以进一步分化成纤维细胞和角质形成细胞。进一步描述的是用于从脐带的羊膜分离干/祖细胞，worin的方法的方法包括从所述脐带的其它组分在体外分离羊膜培养允许细胞增殖的条件下羊膜组织，和分离来自组织培养物中的干细胞/祖细胞。因此，本发明涉及本文皮肤等同物的治疗用途。本发明的另一个方面涉及使用从脐带和寻求的粘蛋白生成细胞的治疗用途的羊膜获得的干/祖细胞粘蛋白生成细胞的产生。在又一方面，本发明涉及一种用于使用从脐带和它们的治疗用途的羊膜分离的干细胞/祖细胞的胰岛素产生细胞的生成的方法。本发明还涉及到一种使用一个UCMC骨或软骨障碍的方法。进一步，本发明涉及到产生多巴胺和酪氨酸羟化酶以及一个HLA-G，并使用UCMC和/或肝细胞UCEC的方法。因此，本发明涉及诱导使用UCMC老化的角质形成细胞的增殖的方法。

18. 发明公开

EP1731177A4 BIOLOGICAL TISSUE SHEET, METHOD OF FORMING THE SAME AND TRANSPLANTATION METHOD BY USING THE SHEET 审中-公开
标题翻译：生物组织面积，用于生产和移植的方法使用这个区域
公开(公告)号：EP1731177A4
公开(公告)日：2009-07-22
申请号：EP05719186
申请日：2005-02-16
申请人： ARBLAST CO LTD , HASHIMOTO KOUJI
发明人： HASHIMOTO KOUJI , SHIRAKATA YUUJI , OHASHI YUUICHI , HAMURO JUNJI
IPC分类号： A61L27/00 , A61L27/38 , C12N5/071
CPC分类号： C12N5/0621 , A61L27/3604 , A61L27/3804 , A61L27/3869 , C12N5/0629 , C12N5/0698 , C12N2502/094 , C12N2502/1323 , C12N2533/92
摘要： A biological tissue sheet which is expected as exerting a favorable therapeutic effect and a high safety in transplantation. The biological tissue sheet formed by (a) preparing in vivo-derived cells; (b) sowing the in vivo-derived cells on amniotic membrane; and (c) culturing and proliferating the in vivo-derived cells in the absence of any xenogeneic animal cells. As the cells of a biological origin, for example, cells originating in corneal epithelium, conjunctival epithelium, skin epidermis, hair follicle epithelium, oral mucosa, respiratory tract mucosa, or intestinal tract mucosa.

19. 发明公开

EP2007879A2 CELL CO-CULTURE 审中-公开
标题翻译：细胞共培养
公开(公告)号：EP2007879A2
公开(公告)日：2008-12-31
申请号：EP07753419.6
申请日：2007-03-15
申请人： Intercytex Limited
发明人： QIAO, Jizeng , TEUMER, Jeffrey, Keeler , PHILIPS, Erica, Jean
IPC分类号： C12N5/06
CPC分类号： A61L27/3886 , A61L27/3813 , A61L2430/18 , C12N5/0627 , C12N5/0628 , C12N5/0656 , C12N5/0666 , C12N2501/415 , C12N2502/02 , C12N2502/091 , C12N2502/092 , C12N2502/094 , C12N2502/1323
摘要： This invention relates to methods of producing hair follicles in vitro, compositions for producing hair follicles in vitro, in vitro produced hair follicles, methods of providing an in vitro produced hair shaft at an interfollicular or intrafollicular site, methods of treating hair loss by providing an in vitro produced hair shaft at an interfollicular or intrafollicular site and assays for studying the effect of test agents on hair biology. The invention also provides the similar methods and products which are, or use, immature follicles ('defined herein as proto- hairs').The invention provides a method for in vitro production of a hair follicle or a proto-hair comprising co-culturing dermal papilla cells with keratinocytes, and optionally with melanocytes.

20. 发明公开

EP2007878A2 AUTOMATABLE ARTIFICIAL IMMUNE SYSTEM (AIS) 审中-公开
标题翻译：自动化仪器仪表（AIS）
公开(公告)号：EP2007878A2
公开(公告)日：2008-12-31
申请号：EP07753216.6
申请日：2007-03-15
申请人： VaxDesign Corporation
发明人： WARREN, William, L. , PARKHILL, Robert , NGUYEN, Michael, N. , SANCHEZ-SCHMITZ, Guzman , FAHLENKAMP, Heather , HIGBEE, Russell , DRAKE, Donald, III , KACHURIN, Anatoly, M. , MOE, David
IPC分类号： C12N5/06 , G01N33/50
CPC分类号： C12N5/0697 , B01L3/5027 , C12N2501/21 , C12N2502/094 , C12N2502/11 , C12N2502/1107 , C12N2502/1114 , C12N2502/1121 , C12N2502/1192 , C12N2502/28 , C12N2503/02 , C12N2503/04 , C12N2531/00 , C12N2533/50 , C12N2533/54 , C12N2535/10 , G01N33/5082 , G01N33/5088
摘要： The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. In particular the claimed artificial immune system comprises a vaccination site, comprising a first matrix and a plurality of cells attached to said matrix, wherein a plurality of said vaccination sites are organized in a multi-well format; and a three-dimensional artificial lymphoid tissue, comprising a second matrix and a plurality of lymphocytes and leukocytes, wherein a plurality of said three-dimensional artificial lymphoid tissues are organized in a second multi-well format. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interactions with the immune system.
摘要翻译：本发明涉及构建综合人造免疫系统的方法，其包括适当的体外细胞和组织构建体或其等同物以模拟与哺乳动物中的疫苗相互作用的正常组织。人造免疫系统可用于测试疫苗候选物在体外的功效，因此可用于加速疫苗开发和测试与免疫系统的药物和化学相互作用。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式