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    • 17. 发明公开
    • Site-directed pegylation of arginases and the use thereof as anti-cancer and anti-viral agents
    • 精氨酸酶的定点聚乙二醇化及其作为抗癌剂和抗病毒剂的用途
    • EP2411040A1
    • 2012-02-01
    • EP10769236.0
    • 2010-03-26
    • The Hong Kong Polytechnic University
    • LEUNG, Thomas, Yun-ChungLO, Thomas, Wai-Hung
    • A61K38/43A61P35/00A61P31/00
    • C12N9/78A61K38/00A61K47/60C12Y305/03001
    • Mono-pegylated arginase conjugate and method producing thereof. The mono-pegylated arginase is homogeneous in molecular weight and shows therapeutic effect for treating cancers and viral infections. The method of producing such arginase conjugate has a main step of genetically modifying the gene encoding an arginase so that the PEG moiety can attach to the enzyme at a predetermined, specific intended site. This is achieved by removing the PEG attaching amino acid residues at undesirable sites while keeping (or adding, if necessary) the one at the desirable site of the enzyme. Two exemplary mono-pegylated arginase conjugates so produced are human arginase I (HAI) where a polyethylene glycol (PEG) moiety is site-specific covalently bonded to Cys
      45 of the enzyme and
      Bacillus caldovelox arginase (BCA) where a polyethylene glycol (PEG) moiety is site-specific covalently bonded to Cys
      161 of the enzyme.
    • 单聚乙二醇化精氨酸酶缀合物及其生产方法。 单聚乙二醇化精氨酸酶在分子量上是均匀的,并显示出治疗癌症和病毒感染的治疗效果。 生产这种精氨酸酶缀合物的方法具有对编码精氨酸酶的基因进行遗传修饰的主要步骤,使得PEG部分可以在预定的特定预定位点附着于酶。 这是通过除去不希望的位点上的PEG连接氨基酸残基,同时保持(或者如果需要,添加)在期望的酶位点处的氨基酸残基。 如此产生的两种示例性单聚乙二醇化精氨酸酶缀合物是其中聚乙二醇(PEG)部分是位点特异性共价结合至酶和Caldovelox精氨酸酶(BCA)的Cys45的人精氨酸酶I(HAI),其中聚乙二醇(PEG) 部分是位点特异性共价键合到酶的Cys161。
    • 19. 发明公开
    • VERTICAL MICROINJECTION MACHINE
    • VERTIKALMIKROINJEKTIONSMASCHINE
    • EP2261001A1
    • 2010-12-15
    • EP09716266.3
    • 2009-03-03
    • The Hong Kong Polytechnic University
    • YUNG, Kai-leung
    • B29C45/64B29C45/03B29C45/74B29C45/77B29C45/76
    • B29C45/66B29C45/1761B29C45/53B29C45/76B29C2045/0094B29C2945/76006B29C2945/76013B29C2945/762B29C2945/7621B29C2945/76381
    • A vertical microinjection machine comprises a base (1) and a driving unit, a plasticizing unit, an injection unit, a mould closing system which are arranged on the base (1), wherein the mould closing system comprises a lower mould plate (2) which is fixed on the base (1) by a bracket arranged on the base (1), an upper mould plate (3) which is movable upward and downward relative to the lower mould plate (2), and two independent mould closing drive units which are symmetrically arranged on the base. Each mould closing drive unit comprises two thrust rods (5), a transmission part, a screw rod, a servo motor (4), a square "□" shaped support frame and a linear guide. The servo motor (4) drives the screw rod, and then brings the transmission part to move up and down. Driven by the transmission mechanism, the thrust rods (5) open and close the upper and lower clamping plates (3, 2) so as to achieve the opening and closing operation.
    • 一种垂直显微注射机,包括一个设置在基座(1)上的基座(1)和一个驱动单元,一个塑化单元,一个注射单元,一个闭合系统,其中该闭模系统包括下模板(2) 其通过布置在基座(1)上的支架固定在基座(1)上,相对于下模板(2)上下移动的上模板(3)和两个独立的闭模驱动单元 它们对称地布置在基座上。 每个闭模驱动单元包括两个推杆(5),传动部分,螺杆,伺服马达(4),正方形“形支撑框架和直线导轨。 伺服电机(4)驱动螺杆,然后使传动部件上下移动。 由传动机构驱动,推杆(5)打开和关闭上下夹板(3,2),以实现打开和关闭操作。