专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

91. 发明公开

EP2062056A2 DIFFERENTIATION OF FLOW CYTOMETRY PULSES AND APPLICATIONS 有权转让
标题翻译：脉冲的术和应用分化
公开(公告)号：EP2062056A2
公开(公告)日：2009-05-27
申请号：EP07843384.4
申请日：2007-09-27
申请人： Guava Technologies, Inc.
发明人： PITTARO, Richard , GOLDMAN, Bruce , LEFEBVRE, Ray , KING, David, A.
IPC分类号： G01P3/36 , G01F1/36
CPC分类号： G01N15/14 , G01N15/1429 , G01N15/1459 , G01N2015/1006 , G01N2015/1477 , G01N2015/1486
摘要： A method of analyzing pulses from a flow cytometer in which particles in a fluid pass through an excitation volume of an electromagnetic radiation and interact with the electromagnetic radiation to generate signals in the form of pulses includes generating a time-dependent pulse indicative of the characteristics of one or more particles passing through the excitation volume of the electromagnetic radiation, determining a measurement window by selecting a portion of the pulse with a starting point and an ending point above a predetermined value, and calculating a first derivative of the pulse with respect to time over the measurement window.

92. 发明公开

EP2045595A2 Cell analyser and cell analysing method 有权
标题翻译： Zellanalysator和Zellanalyseverfahren
公开(公告)号：EP2045595A2
公开(公告)日：2009-04-08
申请号：EP08017313.1
申请日：2008-10-01
申请人： SYSMEX CORPORATION
发明人： Fukuda, Masakazu , Ishisaka, Masaki
IPC分类号： G01N15/14 , G01N21/64
CPC分类号： G01N15/147 , G01N15/1434 , G01N15/1459 , G01N2015/1477 , G01N2021/6439
摘要： The present invention is to present a cell analyzer capable of measuring cells which are approximately 20 to 100 µm in size with high precision via flow cytometry. The cell analyzer 10 comprises: a flow cell 51 in which a measurement sample including a measurement target cell flows; a light source part 53 for irradiating a light on the measurement sample flowing in the flow cell 51; an optical system 52 for forming a beam spot on the measurement sample flowing in the flow cell 51, the beam spot having a diameter of 3∼8 µm in a flow direction of the measurement sample and a diameter of 300∼600 µm in a direction perpendicular to the flow direction of the measurement sample; and a light receiving part 55, 58, 59 for receiving a light from the measurement sample.
摘要翻译：本发明提供一种能够通过流式细胞术以高精度测量大小为约20至100μm的细胞的细胞分析仪。细胞分析装置10包括：流动池51，其中包括测量对象单元的测量样本流动; 用于对在流动池51中流动的测量样品照射光的光源部53; 用于在流动池51中流动的测量样品上形成束斑的光学系统52，在测量样品的流动方向上具有直径为3¼8μm的束斑和在垂直于流动的方向上的直径为300 /600μm的直径测量样品的方向; 以及用于接收来自测量样品的光的光接收部分55,58,59。

93. 发明公开

EP1815232A1 DEVICE AND METHOD FOR MANAGING DATA FROM A FLOW ANALYZER 审中-公开
标题翻译：系统和方法用于管理数据从STRÖMUNGSANALYSATORS
公开(公告)号：EP1815232A1
公开(公告)日：2007-08-08
申请号：EP05851761.6
申请日：2005-11-16
申请人： Luminex Corporation
发明人： MOORE, Douglas, E.
IPC分类号： G01N15/14
CPC分类号： G01N15/14 , G01N2015/1477
摘要： A system and method for processing sample data from a flow analyser (420) employing hardware, such as a Field Programmable Gate Array (FPGA), to process the sample data in small pipelined steps. The processing includes a circular buffer where the read and write of data is synchronous, preventing buffer overrun or data loss. This pipeline processing approach allows increasing data acquisition channels or additional processing steps without limiting processing speed.

94. 发明公开

EP1785899A3 Methods for identifying discrete populations (e.g. clusters) of data within a flow cytometer multi-dimensional data set 有权
标题翻译：用于识别流式细胞仪多维数据集内的数据的离散群体（例如群集）的方法
公开(公告)号：EP1785899A3
公开(公告)日：2007-05-23
申请号：EP06255789.7
申请日：2006-11-10
申请人： IDEXX Laboratories Inc
发明人：
IPC分类号： G06F19/00
CPC分类号： G01N15/1459 , G01N2015/008 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , G06F19/00 , G06K9/00147 , G06K9/6226 , G06K9/626 , G16H50/20 , G16H50/70
摘要： Systems and methods for identifying populations of events in a multi-dimensional data set, e.g., seven dimensional flow cytometry data of a blood sample. The populations may, for example, be sets or clusters of data representing different white blood cell components in the sample. The methods use a library consisting of one or more one finite mixture models, each model component comprising parameters representing multi-dimensional Gaussian probability density functions, one density for each population of events expected in the data set. The methods further use an expert knowledge set comprising one or more data transformations for operation on the multi-dimensional data set and one or more logical statements. The transformations and logical statements encode a priori expectations as to the relationships between different event populations in the data set. The methods further use program code comprising instructions by which a processing unit such as a computer may operate on the multi-dimensional data, a finite mixture model selected from the library, and the expert knowledge set to thereby identify populations of events in the multi-dimensional data set.
摘要翻译：用于鉴定多维数据集中的事件群体的系统和方法，例如血液样本的七维流式细胞术数据。例如，群体可以是代表样本中不同白血细胞组分的数据集或数据集。这些方法使用由一个或多个有限混合模型组成的库，每个模型组件包括表示多维高斯概率密度函数的参数，对于数据集中预期的每个事件群体，一个密度。该方法进一步使用专家知识集合，其包括用于在多维数据集上操作的一个或多个数据变换以及一个或多个逻辑语句。这些转换和逻辑陈述对数据集中不同事件群体之间的关系进行先验预期编码。所述方法进一步使用程序代码，所述程序代码包括指令，诸如计算机的处理单元可以通过所述指令对多维数据，从所述库中选择的有限混合模型以及所述专家知识集合进行操作，从而识别所述多维数据集中的事件群体，三维数据集。

95. 发明公开

EP1542008A4 METHOD OF CLASSIFYING AND COUNTING LEUCOCYTES 有权
标题翻译：分类程序和白细胞计数
公开(公告)号：EP1542008A4
公开(公告)日：2006-07-12
申请号：EP03733538
申请日：2003-06-23
申请人： SYSMEX CORP
发明人： TSUJI TOMOHIRO , MIZUKAMI TOSHIHIRO , KONISHI AYA , MORI YUSUKE , NAKAZAWA YUKIE
IPC分类号： G01N33/52 , G01N15/00 , G01N15/14 , G01N21/64
CPC分类号： G01N33/52 , G01N2015/1477
摘要： A method of classifying and counting leucocytes with abnormal DNA content which comprises: (1) the step of treating a hematological sample with a hemolytic agent and staining cells in the thus hemolyzed sample with a fluorescent dye capable of showing a difference in fluorescent intensities of at least matured leucocytes, leucocytes with abnormal DNA content and immature leucocytes; (2) the step of introducing the sample containing the stained cells into a flow cytometer and measuring the scattering light and the fluorescence of each cell; (3) the step of separating leucocytes from aggregated platelets and simultaneously passing cells with the use of a difference in scattering light peaks and a difference in scattering light widths ; and (4) classifying the leucocytes separated in the step (3) into mature leucocytes, leucocytes with abnormal DNA content and immature leucocytes with the use of a difference in scattering light intensities and a difference in fluorescent intensities of the cells followed by counting.

96. 发明公开

EP1616286A1 METHOD OF, AND COMPUTER SOFTWARE FOR, CLASSIFICATION OF CELLS INTO SUBPOPULATIONS 审中-公开
标题翻译： VERFAHREN UND COMPUTERSOFTWARE ZUR KLASSIFIKATION VON ZELLEN ZU SUBPOPULATIONEN
公开(公告)号：EP1616286A1
公开(公告)日：2006-01-18
申请号：EP04725411.5
申请日：2004-04-02
申请人： Amersham Biosciences UK Limited
发明人： ARINI, Nicholas, Amersham Biosciences UK Limited , GOODYER, Ian David, Amersham Biosciences UK Ltd. , MURPHY, Samantha, Amersham Biosciences UK Limited , ROQUEMORE, Elizabeth, Amersham Biosciences UK Ltd , ZALTSMAN, Alla, Amersham Biosciences UK Limited , ALEXANDROV, Yuriy, c/o Amersham Biosciences UK Ltd , DAGENAIS, Louis, Amersham Biosciences Niagara Inc , SOLTYS, Bohdan, Amersham Biosciences Niagara Inc. , CYBUCH, Jurek
IPC分类号： G06K9/00 , G01N15/14 , G01N21/76
CPC分类号： G06K9/00127 , G01N15/1475 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2015/1477 , G01N2015/1493
摘要： A method of classifying cells into subpopulations using cell classifying data is described. The method comprises receiving and analyzing image data to identify object areas in the image data to determine, for at least one selected first cell, one or more measurements. A first parameter set is derived from the measurements for the first cell, the first parameter set comprising at least one of said one or more measurements. The first set of cells are classified into subpopulations, and identified to produce first identifying data. Cell classifying data for use in classifying a second set of cells into subpopulations is derived from the first parameter set and the first identifying data. A second set of cells is classified into subpopulations on the basis of one or more measurements taken for cells in the second set of cells, by use of the cell classifying data. The parameter sets of cells may be represented as vectors in an n-dimensional space.
摘要翻译：描述了使用细胞分类数据将细胞分类为亚群体的方法。该方法包括接收和分析图像数据以识别图像数据中的对象区域，以针对至少一个所选择的第一小区确定一个或多个测量。从第一单元的测量中导出第一参数集，第一参数集包括所述一个或多个测量中的至少一个。第一组细胞被分类为亚群，并被鉴定以产生第一识别数据。从第一参数集和第一识别数据导出用于将第二组细胞分类为子群体的细胞分类数据。通过使用细胞分类数据，基于通过第二组细胞中的细胞进行的一个或多个测量将第二组细胞分类为亚群体。单元的参数集可以表示为n维空间中的向量。

97. 发明公开

EP1590652A1 MEHRPARAMETRIGES ZELL-IDENTIFIKATIONS- UND SORTIERVERFAHREN UND ZUGEHÖ RIGE VORRICHTUNG 审中-公开
标题翻译： MEHRPARAMETRIGES小区标识，以及排序方法和设备ZUGEHÖ双管齐下
公开(公告)号：EP1590652A1
公开(公告)日：2005-11-02
申请号：EP04707905.8
申请日：2004-02-04
申请人： Evotec Technologies GmbH
发明人： MÜLLER, Torsten , HUMMEL, Stefan , PFENNIG, Annette , GRADL, Gabriele , BONSEN, Axel , SCHNELLE, Thomas , MEYER, Rüdiger
IPC分类号： G01N15/14
CPC分类号： G01N15/1459 , C12M47/04 , G01N15/1056 , G01N15/12 , G01N15/147 , G01N15/1484 , G01N2015/1006 , G01N2015/1037 , G01N2015/1081 , G01N2015/1477 , G01N2015/149 , G01N2015/1497 , G01N2035/00158
摘要： The invention relates to a method for analyzing biological particles, particularly to be carried out in a cell sorter, comprising the following steps: the particles that are to be analyzed are introduced into a carrier flow; a first analysis of the particles moving along with the carrier flow is performed; at least one particle is selected according to the result of the first analysis; the selected particle is slowed down; a second analysis of the selected particle is performed in the decelerated state thereof. The invention also relates to a corresponding analyzing device.

98. 发明公开

EP1381846A2 VERFAHREN ZUR ANALYSE EINER BIOLOGISCHEN PROBE 有权
标题翻译： VERFAHREN ZUR分析EINER BIOLOGISCHEN探针
公开(公告)号：EP1381846A2
公开(公告)日：2004-01-21
申请号：EP02745294.5
申请日：2002-05-14
申请人： FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DERANGEWANDTEN FORSCHUNG E.V.
发明人： SCHMIDT, Robert , WITTENBERG, Thomas , GERHÄUSER, Heinz , SPINNLER, Klaus
IPC分类号： G01N15/14 , G06F19/00
CPC分类号： G06K9/00127 , G01N15/1468 , G01N15/1475 , G01N2015/1477 , G01N2015/1488 , G01N2015/1497 , G06T7/0012
摘要： The invention relates to a method for analyzing a biological sample (104') to determine a modification of said sample in comparison with a healthy biological sample. Said method involves producing a first image of the biological sample in a first imaging modality (DL) and producing a second image of the biological sample in a second imaging modality (PK). The first image is classified (K1)on the basis of a first predetermined parameter while the second image is classified (K2) on the basis of a second predetermined parameter, wherein the first image is classified independently of the second image. Depending on classification results (E1, E2), it is then determined whether the biological sample (104') shows any modification in comparison with a healthy biological sample.
摘要翻译：本发明涉及用于分析生物样品（104'）以确定与健康生物样品相比所述样品的修饰的方法。所述方法涉及在第一成像模态（DL）中产生生物样品的第一图像并且在第二成像模态（PK）中产生生物样品的第二图像。第一图像基于第一预定参数被分类（K1），而第二图像基于第二预定参数被分类（K2），其中第一图像被独立于第二图像而被分类。根据分类结果（E1，E2），然后确定生物样品（104'）与健康生物样品相比是否显示任何修饰。

99. 发明公开

EP1363126A1 Multidimensional leukocyte differential analysis 有权
标题翻译：多维度分析von Leukozyten
公开(公告)号：EP1363126A1
公开(公告)日：2003-11-19
申请号：EP02380098.0
申请日：2002-05-14
申请人： Universidad De Salamanca
发明人： Orfao de Matos Correia e Valle, Alberto
IPC分类号： G01N33/569 , G01N33/58
CPC分类号： G01N33/56972 , G01N15/1459 , G01N33/582 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331 , Y10T436/13
摘要： This invention relates to an improved procedure for the multiparameter analysis of leukocyte subpopulations from peripheral blood, bone marrow or any body fluid containing blood nucleated cells, including the quantification of each subpopulation in terms of cells per microliter. The method uses a cocktail of at least four monoclonal antibodies labelled each with a different fluorescent tag, the measurement of at least two light scatter and four fluorescence emissions related parameters and at least one population of fluorescent beads to differentiate and enumerate between and among the different leukocyte subpopulations in the peripheral blood, the bone marrow or other body fluid. It includes: the incubation of the sample with a mixture of monoclonal antibodies conjugated to a minimum of four different fluorochromes, the addition of one or more populations of reference beads, the flow cytometry measurement of the fluorescence emissions associated to each cell and bead population and, the mutidimesional analysis of each cell and bead population present in the sample aimed at its identification, enumeration and characterization.
摘要翻译：本发明涉及一种用于多参数分析来自外周血，骨髓或含有血液有核细胞的任何体液的白细胞亚群的改进方法，包括以每微升细胞计数每个亚群。该方法使用至少四种单克隆抗体的混合物，每个单克隆抗体标记各自具有不同的荧光标签，测量至少两个光散射和四个荧光发射相关参数，以及至少一个荧光珠群，以区分和枚举不同的白细胞亚群在外周血，骨髓或其他体液中。其包括：将样品与缀合至少四种不同荧光染料的单克隆抗体的混合物孵育，加入一个或多个参比珠群，与每个细胞和珠粒相关联的荧光发射的流式细胞术测量，以及，样本中存在的每个细胞和珠粒的多时间分析旨在其鉴定，计数和表征。

100. 发明公开

EP1068514A1 DUAL LARGE ANGLE LIGHT SCATTERING DETECTION 审中-公开
标题翻译：光散射在两个大角度证明
公开(公告)号：EP1068514A1
公开(公告)日：2001-01-17
申请号：EP99954860.5
申请日：1999-10-12
申请人： University of Washington
发明人： ALTENDORF, Eric, H.
IPC分类号： G01N21/64
CPC分类号： G01N15/147 , G01N21/21 , G01N2015/008 , G01N2015/1477 , G01N2015/1486 , G01N2015/1497
摘要： An optical analyzer with a configuration particularly suitable for use with planar liquid sample flow cells (1) is provided comprising a polarized light source (10) and at least two large angle scattered light photodetectors (20, 30) positioned respectively at acute, and right or oblique angles to the incident light beams. Differences in intensities of light measured at the two photodetectors (20, 30) are used to quantify components of the sample (1).

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式