基本信息:
- 专利标题： MULTIPURPOSE REAGENT SYSTEM FOR RAPID LYSIS OF WHOLE BLOOD SAMPLES
- 专利标题（中）：多用途试剂快速裂解全血标本
- 申请号：EP94909551.7 申请日：1994-02-03
- 公开(公告)号：EP0685994B1 公开(公告)日：2007-03-21
- 发明人： KIM, Young, Ran , KANTOR, Joanna
- 申请人： ABBOTT LABORATORIES
- 申请人地址： CHAD-0377/AP6D-2 One Abbott Park Road Abbott Park, IL 60064-3500 US
- 专利权人： ABBOTT LABORATORIES
- 当前专利权人： ABBOTT LABORATORIES
- 当前专利权人地址： CHAD-0377/AP6D-2 One Abbott Park Road Abbott Park, IL 60064-3500 US
- 代理机构： Modiano, Micaela Nadia
- 优先权： US23042 19930225
- 国际公布： WO1994018828 19940901
- 主分类号： A01N1/02
- IPC分类号： A01N1/02 ; C12Q1/02 ; C12Q1/06 ; G01N1/28 ; G01N15/10 ; G01N21/64 ; G01N33/49 ; G01N33/533 ; G01N33/536 ; G01N33/50 ; G01N33/569 ; G01N1/30
摘要：
A multipurpose reagent system for rapid analysis of a whole blood sample allowing the determination of at least five classes of peripheral white blood cells, nucleated red blood cells, and lymphocyte immunophenotyping on automated hematology instrumentation. The multipurpose reagent system lyses red cells rapidly, while it concurrently fixes white cells and preserves surface antigens on lymphocytes. The multipurpose reagent system comprises from about 3 to 7 grams per liter of a non-quaternary ammonium salt, from about 0.04 to about 0.10 percent by volume of an aliphatic aldehyde with one to four carbons, from about 10 mM to about 20 mM of a non-phosphate buffer which is inert to the aliphatic aldehyde, and a sufficient amount of water to give a pH between 5.5 and 7.5 and an osmolality of between about 160 to about 310 mOsm per liter.