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1. 发明授权

EP2256188B1 METHOD FOR INCREASING ENZYMATIC REACTIVITY 有权
标题翻译：法提高酶促反应性
公开(公告)号：EP2256188B1
公开(公告)日：2014-06-18
申请号：EP09714443.0
申请日：2009-02-27
申请人： Riken , Dnaform
发明人： KOJIMA, Miki , CARNINCI, Piero , HAYASHIZAKI, Yoshihide , HARBERS, Matthias
IPC分类号： C12N9/00 , C12N15/09 , C12Q1/68
CPC分类号： C12N9/96 , C12Q1/6834 , C12Q1/686 , C12Q1/6869 , C12Q2527/127 , C12Q2527/125 , C12Q2521/543

2. 发明授权

EP1712618B1 METHOD OF AMPLIFYING NUCLEIC ACID AND METHOD OF DETECTING MUTATED NUCLEIC ACID USING THE SAME 有权转让
标题翻译：核酸的方法扩增和检测方法的突变核酸使用相同的
公开(公告)号：EP1712618B1
公开(公告)日：2014-06-04
申请号：EP04807703.6
申请日：2004-12-24
申请人： RIKEN , Kabushiki Kaisha Dnaform
发明人： MITANI, Yasumasa, c/o Wakunaga Pharm. Co., Ltd. , OKA, Takanori, c/o Wakunaga Pharm. Co., Ltd , HAYASHIZAKI, Yoshihide , HAYASHI, Toshizo, c/o K.K. Dnaform
IPC分类号： C12N15/00 , C12Q1/68 , G01N33/53 , G01N33/566
CPC分类号： C12Q1/6853 , C12Q1/6816 , C12Q1/6827 , C12Q1/6858 , C12Q2537/163 , C12Q2531/113 , C12Q2521/514 , C12Q2525/301 , C12Q2525/161 , C12Q2537/125 , C12Q2522/101

3. 发明公开

EP1661988A1 KIT FOR NUCLEIC ACID DETECTION 审中-公开
标题翻译：核酸检测试剂盒
公开(公告)号：EP1661988A1
公开(公告)日：2006-05-31
申请号：EP04748062.9
申请日：2004-07-29
申请人： RIKEN , Kabushiki Kaisha Dnaform , WAKUNAGA PHARMACEUTICAL CO., LTD.
发明人： MITANI, Yasumasa , OKA, Takanori , HAYASHIZAKI, Yoshihide , HAYASHI, Toshizo
IPC分类号： C12N15/00 , C12M1/00 , C12Q1/68 , G01N33/50
CPC分类号： B01L3/502 , B01L3/5082 , B01L2200/0621 , B01L2300/0672 , B01L2300/0832 , B01L2300/087 , B01L2400/0677 , B01L2400/0683
摘要： The present invention provides a reactor for carrying out in a single container the steps of the extraction of nucleic acids and the amplification of a nucleic acid, the procedures of the steps being usually different to each other. The reactor according to the present invention is a reactor for detecting a target nucleic acid from a sample, comprising at least a first compartment which contains an extraction reagent composition for extracting nucleic acids from said sample, a second compartment which contains an amplification reagent composition for amplifying the target nucleic acid, a separating means for separating the first and second compartments, and an aperture which enables to introduce said sample into only said first compartment. The separating means breaks the separation of the first and second compartments by physical energy supplied from the outside of the reactor, and thereby makes it possible to mix the extraction reagent composition in the first compartment and the amplification reagent composition in the second compartment.
摘要翻译：本发明提供了用于在单一容器中进行核酸提取和核酸扩增的步骤的反应器，这些步骤的步骤通常彼此不同。根据本发明的反应器是用于检测来自样品的靶核酸的反应器，其至少包含第一隔室和第二隔室，第一隔室含有用于从所述样品中提取核酸的提取试剂组合物，第二隔室含有扩增试剂组合物，扩增靶核酸，用于分离第一和第二隔室的分离装置以及能够仅将所述样品引入所述第一隔室的孔。分离装置通过从反应器外部供应的物理能量破坏第一和第二隔室的分离，从而使得可以将第一隔室中的提取试剂组合物和第二隔室中的扩增试剂组合物混合。

4. 发明公开

EP1451317A1 METHODS OF STORING AND/OR DELIVERING AN OLIGOMER AND/OR POLYMER APPLIED ON A SUPPORT, AND SUPPORTS THEREOF 审中-公开
标题翻译：于存储和/或喂养AN在载波方法来施加低聚物和/或聚合物与性载体
公开(公告)号：EP1451317A1
公开(公告)日：2004-09-01
申请号：EP02775496.9
申请日：2002-11-05
申请人： Riken , Kabushiki Kaisha Dnaform
发明人： HAYASHIZAKI, Yoshihide
IPC分类号： C12N15/00 , C12Q1/68 , G01N33/53 , C12M1/00
CPC分类号： B01L3/5055 , B01J19/0046 , B01J2219/00373 , B01J2219/00378 , B01J2219/00385 , B01J2219/00387 , B01J2219/00497 , B01J2219/00527 , B01J2219/00542 , B01J2219/00547 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00637 , B01J2219/00641 , B01J2219/00644 , B01J2219/00657 , B01J2219/00659 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01J2219/00731 , B01L9/50 , B01L2300/069 , C12Q1/6806 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , G01N35/00009 , C12Q2565/518
摘要： A method for storing and/or delivering an oligomer and/or polymer applied on at least one support, comprising the steps of:(a) applying at least one oligomer and/or polymer on at least one support; (b) folding or rolling the support; and(c) storing and/or delivering the support of step (b)is provided. Also, a support having at least one oligomer and/or polymer applied thereon, which is in the form of a folded or rolled sheet, a loose-leaf sheet or a card and a water-soluble support having at least one oligomer and/or polymer applied thereon are provided.According to the present invention, molecular substances (oligomers and/or polymers) can be stored and deliveredin a simple system. This system can preserve the substances without contamination and degradation.

5. 发明公开

EP2256188A1 METHOD FOR INCREASING ENZYMATIC REACTIVITY 有权
标题翻译： VERFAHREN ZURERHÖHUNGDER ENZYMATISCHENREAKTIVITÄT
公开(公告)号：EP2256188A1
公开(公告)日：2010-12-01
申请号：EP09714443.0
申请日：2009-02-27
申请人： Riken , Dnaform
发明人： KOJIMA, Miki , CARNINCI, Piero , HAYASHIZAKI, Yoshihide , HARBERS, Matthias
IPC分类号： C12N9/00 , C12N15/09 , C12Q1/68
CPC分类号： C12N9/96 , C12Q1/6834 , C12Q1/686 , C12Q1/6869 , C12Q2527/127 , C12Q2527/125 , C12Q2521/543
摘要： An object of the invention is to provide a method for increasing enzymatic reactivity to a target substance immobilized on a support; and a method for reducing or suppressing an inhibitory effect of a support on enzymatic reaction. The above object is achieved by a method for increasing enzymatic reactivity to a target substance immobilized on a support by allowing at least one substance selected from the group consisting of saccharides, amino acids, polyhydric alcohols and derivatives thereof to exist; and a method for reducing or suppressing an inhibitory effect of a support immobilized with a target substance on enzymatic reactivity to the target substance by allowing at least one substance selected from the group consisting of saccharides, amino acids, polyhydric alcohols and derivatives thereof to exist.
摘要翻译：本发明的目的是提供一种增加对固定在载体上的目标物质的酶反应性的方法; 以及减少或抑制载体对酶促反应的抑制作用的方法。上述目的是通过使至少一种选自由糖，氨基酸，多元醇及其衍生物组成的组中选出的物质存在而提高对固定在载体上的目标物质的酶反应性的方法来实现的。以及通过使至少一种选自由糖，氨基酸，多元醇及其衍生物组成的组的物质存在的方式，减少或抑制固定有靶物质的载体对目标物质的酶反应性的抑制作用。

6. 发明公开

EP1712618A1 METHOD OF AMPLIFYING NUCLEIC ACID AND METHOD OF DETECTING MUTATED NUCLEIC ACID USING THE SAME 有权转让
标题翻译：核酸的方法扩增和检测方法的突变核酸使用相同的
公开(公告)号：EP1712618A1
公开(公告)日：2006-10-18
申请号：EP04807703.6
申请日：2004-12-24
申请人： RIKEN , Kabushiki Kaisha Dnaform
发明人： MITANI, Yasumasa, c/o Wakunaga Pharm. Co., Ltd. , OKA, Takanori, c/o Wakunaga Pharm. Co., Ltd , HAYASHIZAKI, Yoshihide , HAYASHI, Toshizo, c/o K.K. Dnaform
IPC分类号： C12N15/00 , C12Q1/68 , G01N33/53 , G01N33/566
CPC分类号： C12Q1/6853 , C12Q1/6816 , C12Q1/6827 , C12Q1/6858 , C12Q2537/163 , C12Q2531/113 , C12Q2521/514 , C12Q2525/301 , C12Q2525/161 , C12Q2537/125 , C12Q2522/101
摘要： A primer set that allows a target nucleic acid to be amplified specifically and efficiently. The primer set of the present invention includes at least two primers that allow a target nucleic acid sequence to be amplified. A first primer included in the primer set contains, in its 3' end portion, a sequence (Ac') that hybridizes to a sequence (A) located in the 3' end portion of the target nucleic acid sequence. The first primer also contains, on the 5' side of the sequence (Ac'), a sequence (B') that hybridizes to a complementary sequence (Bc) to a sequence (B) that is present on the 5' side with respect to the sequence (A) in the target nucleic acid sequence. A second primer included in the primer set contains, in its 3' end portion, a sequence (Cc') that hybridizes to a sequence (C) located in the 3' end portion of a complementary sequence to the target nucleic acid sequence. The second primer also contains, on the 5' side of the sequence (Cc'), a folded sequence (D-Dc') that contains, on the same strand, two nucleic acid sequences that hybridize to each other.
摘要翻译：一种引物组确实允许靶核酸可以特异且高效地扩增。所述引物组，本发明的包括至少两个引物确实允许目标核酸序列进行扩增。包括在所述引物组的第一引物包含在其3“端部分，一序列（AC”）没有杂交到位于靶核酸序列的3“端部分序列（A）。因此，第一引物含有，对“（，序列（B”）的5序列AC）的侧“做杂交的互补序列（BC）的序列（B）那样存在于5”侧相对于在靶核酸序列的序列（A）。包括在所述引物组的第二引物含有，在其3“端部分，一序列（CC”）没有杂交到位于互补序列与靶核酸序列的3“端部分的序列（C）。因此，第二引物含有，对“（5序列CC）的侧”，折叠序列（D-DC“）确实包含，在相同的海滩，两个核酸序列杂交并海誓山盟。

7. 发明公开

EP2371951A1 NOVEL MutS PROTEIN AND METHOD OF USING SAME TO DETERMINE MUTATIONS 审中-公开
标题翻译：神经损伤突变体蛋白在西班牙语VERWENDUNG ZUR BESTIMMUNG VON MUTATIONEN
公开(公告)号：EP2371951A1
公开(公告)日：2011-10-05
申请号：EP09829167.7
申请日：2009-11-27
申请人： Riken , Kabushiki Kaisha Dnaform
发明人： HAYASHIZAKI, Yoshihide , ITOH, Masayoshi , USUI, Kengo , MOMURA, Kazuhito , KANAMORI, Hajime
IPC分类号： C12N15/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12P21/02 , C12Q1/48 , C12Q1/68
CPC分类号： C07K14/195 , G01N33/5308 , G01N2333/32
摘要： A method for determining the presence or absence of a mutation on the basis of the presence or absence of amplification with high reliability is provided. A target sequence including a target site contained in a sample nucleic acid is amplified using a primer that can hybridize to a region including the target site contained in the sample nucleic acid in the presence of a novel MutS having an amino acid sequence of SEQ ID NO: 2, and then the presence or absence of a mutation at the target site is determined on the basis of the presence or absence of amplification. The novel MutS binds more specifically to a mismatched base pair than to a fully-matched base pair, whereby an extension reaction caused by a mismatch-binding primer is suppressed. Thus, according to the present invention, the presence or absence of a mutation can be determined with high reliability.
摘要翻译：提供了一种基于高可靠性的扩增的存在或不存在来确定突变的存在或不存在的方法。包含样品核酸中包含的靶位点的靶序列使用可以在含有SEQ ID NO的氨基酸序列的新型MutS的存在下与包含在样品核酸中的靶位点的区域杂交的引物进行扩增：2，然后基于扩增的存在或不存在来确定靶位点处的突变的存在或不存在。新的MutS更具体地结合失配的碱基对而不是完全匹配的碱基对，由此抑制由错配结合引物引起的延伸反应。因此，根据本发明，可以高可靠性确定是否存在突变。

8. 发明公开

EP1674569A1 SUPPORT HAVING ENZYME IMMOBILIZED THEREON, PRINT, REAGENT KIT, PROCESS FOR PRODUCING THE SUPPORT, METHOD OF PRESERVING ENZYME AND METHOD OF REVITALIZING ENZYME 审中-公开
标题翻译：与事实固定化酶，压力，试剂盒，用于生产的载体，方法用于保存酶AND METHOD FOR振兴酶的CARRIER
公开(公告)号：EP1674569A1
公开(公告)日：2006-06-28
申请号：EP04788311.1
申请日：2004-09-29
申请人： RIKEN , Kabushiki Kaisha Dnaform
发明人： HAYASHIZAKI, Yoshihide , KAMIYA, Mamoru, c/o Kabushiki Kaisha Dnaform
IPC分类号： C12N15/10 , C12N9/12 , C12N11/02 , C12M1/00 , C12Q1/68
CPC分类号： C12N9/1252 , C12N9/96 , C12N11/12
摘要： The invention enables enzymes to be stored in a simple manner. Disclosed are a support having an enzyme and a protecting agent for the enzyme fixed thereon; a printed material and a reagent kit comprising the support; a method for preparing the support; a method for restoring the enzyme fixed on the support; and a method for storing an enzyme in the form of being fixed on a support as a mixture with a protecting agent for the enzyme.
摘要翻译：本发明使酶将被存储在一个简单的方式。本发明公开了具有上酶和支持固定在其上的酶的保护剂; 印刷材料和试剂盒包含载体; 制备该载体的方法; 用于恢复固定在载体上的酶的方法; 并固定在载体上，与酶的保护剂的混合物中的形式的酶的存储的方法。

9. 发明公开

EP1523554A2 METHOD OF UTILIZING THE 5' END OF TRANSCRIBED NUCLEIC ACID REGIONS FOR CLONING AND ANALYSIS 审中-公开
标题翻译：使用5“转录的方法结束克隆和分析NUCLEIC地区
公开(公告)号：EP1523554A2
公开(公告)日：2005-04-20
申请号：EP03733397.8
申请日：2003-06-12
申请人： Riken , Kabushiki Kaisha Dnaform
发明人： HAYASHIZAKI, Yoshihide , CARNINCI, Piero , HARBERS, Matthias T.,c/o KABUSHIKI KAISHA DNAFORM
IPC分类号： C12N15/00
CPC分类号： C12N15/1093 , C12N15/1096
摘要： A method is disclosed for obtaining the 5'ends of transcribed regions from a plurality of nucleic acid fragments obtained from biological materials or synthetic pools. DNA fragments encoding the 5'ends are enriched for their individual analysis or for the analysis of concatemers thereof. The sequence information derived from 5' ends can be used for characterization and cloning of the transcriptome.

10. 发明公开

EP1430462A1 PRINTED MATERIALS COMPRISING A SUPPORT HAVING AN OLIGOMER AND/OR A POLYMER APPLIED THEREON, A METHOD FOR PREPARING THE SAME AND A METHOD FOR DELIVERING AND/OR STORING THE SAME 审中-公开
标题翻译：与一个事实，应用低聚物和/或聚合物，处理车辆印花物料制作SELBIGEM和方法提供和/或存储SELBIGEM
公开(公告)号：EP1430462A1
公开(公告)日：2004-06-23
申请号：EP02765614.9
申请日：2002-09-24
申请人： Kabushiki Kaisha Dnaform , Riken
发明人： HAYASHIZAKI, Yoshihide
IPC分类号： G09F5/04 , G01N33/53 , C12N15/09 , B42D1/00 , B42D15/00
CPC分类号： B01J19/0046 , B01J2219/00373 , B01J2219/00378 , B01J2219/00387 , B01J2219/00527 , B01J2219/00547 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01J2219/00731 , B42D15/0086 , C07B2200/11 , C07K14/59 , C40B40/00
摘要： A printed material comprising at least one support having at least one oligomer and/or polymer applied thereon is provided. Also, a method for preparing the printed material and a method for delivering and storing at least one oligomer and/or polymer are provided. The printed materials of the present invention are useful in providing scientists with oligomers and/or polymers of interest from the printed materials easily and immediately.

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