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1. 发明公开

EP1661988A1 KIT FOR NUCLEIC ACID DETECTION 审中-公开
标题翻译：核酸检测试剂盒
公开(公告)号：EP1661988A1
公开(公告)日：2006-05-31
申请号：EP04748062.9
申请日：2004-07-29
申请人： RIKEN , Kabushiki Kaisha Dnaform , WAKUNAGA PHARMACEUTICAL CO., LTD.
发明人： MITANI, Yasumasa , OKA, Takanori , HAYASHIZAKI, Yoshihide , HAYASHI, Toshizo
IPC分类号： C12N15/00 , C12M1/00 , C12Q1/68 , G01N33/50
CPC分类号： B01L3/502 , B01L3/5082 , B01L2200/0621 , B01L2300/0672 , B01L2300/0832 , B01L2300/087 , B01L2400/0677 , B01L2400/0683
摘要： The present invention provides a reactor for carrying out in a single container the steps of the extraction of nucleic acids and the amplification of a nucleic acid, the procedures of the steps being usually different to each other. The reactor according to the present invention is a reactor for detecting a target nucleic acid from a sample, comprising at least a first compartment which contains an extraction reagent composition for extracting nucleic acids from said sample, a second compartment which contains an amplification reagent composition for amplifying the target nucleic acid, a separating means for separating the first and second compartments, and an aperture which enables to introduce said sample into only said first compartment. The separating means breaks the separation of the first and second compartments by physical energy supplied from the outside of the reactor, and thereby makes it possible to mix the extraction reagent composition in the first compartment and the amplification reagent composition in the second compartment.
摘要翻译：本发明提供了用于在单一容器中进行核酸提取和核酸扩增的步骤的反应器，这些步骤的步骤通常彼此不同。根据本发明的反应器是用于检测来自样品的靶核酸的反应器，其至少包含第一隔室和第二隔室，第一隔室含有用于从所述样品中提取核酸的提取试剂组合物，第二隔室含有扩增试剂组合物，扩增靶核酸，用于分离第一和第二隔室的分离装置以及能够仅将所述样品引入所述第一隔室的孔。分离装置通过从反应器外部供应的物理能量破坏第一和第二隔室的分离，从而使得可以将第一隔室中的提取试剂组合物和第二隔室中的扩增试剂组合物混合。

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