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1. 发明专利

AU2001249072B2 Lipase-containing composition and methods of use thereof 未知
公开(公告)号：AU2001249072B2
公开(公告)日：2004-09-30
申请号：AU2001249072
申请日：2001-02-23
申请人： LILLY CO ELI
发明人： MARGOLIN ALEX , SHENOY BHAMI
IPC分类号： A61K38/46 , A61K38/47 , A61K38/48 , A61P1/14 , A61P1/18 , C12N9/20 , C12N9/26 , C12N9/50 , C12N9/96 , A61K038/46 , A61K038/47 , A61K038/48 , A61P001/00 , A61P003/00 , C12N009/96

2. 发明申请

US20020155562A1 Glutathionylspermidine synthetase and processes for recovery and use thereof 审中-公开
标题翻译：谷胱甘肽合成酶及其回收利用方法
公开(公告)号：US20020155562A1
公开(公告)日：2002-10-24
申请号：US09954433
申请日：2001-09-17
申请人： Leopold Flohe
发明人： Leopold Flohe , Kerstin Koenig , Ulrich Menge
IPC分类号： C07H021/04 , C12N009/00 , C12N009/96 , C12N001/00 , C12N001/10 , C07K002/00 , C07K004/00 , C07K005/00 , C07K007/00 , C07K014/00 , C07K016/00 , C07K017/00 , A61K038/00 , C07K001/00
CPC分类号： C12N9/93 , A61K38/00 , Y10S435/947
摘要： The present invention describes an enzyme showing glutathionylspermidine synthetase-activity and being distinct from known enzymes with similar activities in several physicochemical parameters, a novel process to isolate said enzyme from Crithidia fasciculata, tools for the production thereof in genetically transformed organisms, and its use as a molecular target for the discovery of trypanocidal drugs.
摘要翻译：本发明描述了一种酶，其显示出谷胱甘肽精氨酸合成酶活性，并且与几种物理化学参数中具有相似活性的已知酶不同，一种将所述酶与Crithidia fasciculata分离的新方法，用于在遗传转化的生物中生产的工具及其用途发现杀真菌药物的分子靶点。

3. 发明申请

US20020058319A1 Stabilisation of enzymes during freezing 有权
标题翻译：冻结过程中酶的稳定
公开(公告)号：US20020058319A1
公开(公告)日：2002-05-16
申请号：US09949757
申请日：2001-09-12
申请人： RANDOX LABORATORIES LTD.
发明人： Stephen Peter Fitzgerald , John Campbell , John Victor Lamont , Marlene King
IPC分类号： C12N009/96 , A61K038/54
CPC分类号： C12N9/0065 , C12N9/0006 , C12N9/96
摘要： A method for stabilizing an enzyme during freezing, wherein the enzyme is in a zwitterionic buffer solution. The zwitterionic buffer is able to maintain the activity of the enzyme during freezing and thawing.
摘要翻译：一种用于在冷冻期间稳定酶的方法，其中所述酶在两性离子缓冲溶液中。两性离子缓冲液能够在冷冻和解冻期间保持酶的活性。

4. 发明申请

US20030092892A1 MULTIVALENT ANTIGEN-BINDING PROTEINS 有权
标题翻译：多功能抗体结合蛋白
公开(公告)号：US20030092892A1
公开(公告)日：2003-05-15
申请号：US09530139
申请日：2000-04-27
发明人： LEON GERARDUS FRENKEN , STEVEN HOWELL , ADRIANUS MARINUS LEDEBOER , COMELIS P VAN DER LOGT
IPC分类号： C12N015/09 , C12N009/96 , C07K016/00 , C12P021/08
CPC分类号： G01N33/531 , A61K38/00 , C07K16/00 , C07K16/1275 , C07K16/26 , C07K16/44 , C07K16/468 , C07K2317/22 , C07K2317/34 , C07K2317/569 , C07K2319/00 , Y10S977/927
摘要： A multivalent antigen binding protein comprises a single polypeptide chain comprising, in series, two or more single domain binding units which are preferably heavy chain variable domains derived from an immunoglobulin naturally devoid of light chains. Methods for their production and uses thereof, in particular for diagnosis, immunoassay and purification methods are disclosed.
摘要翻译：多价抗原结合蛋白包含单个多肽链，其包含两个或更多个单结构域结合单元，其优选是衍生自天然缺乏轻链的免疫球蛋白的重链可变结构域。公开了其生产方法及其用途，特别是用于诊断，免疫测定和纯化方法。

5. 发明申请

US20020039771A1 METHOD FOR PRODUCING COMPLEX MULTIENZYMATICAL, STORAGE RESISTANT REACTION MIXTURES AND USE THEREOF 无效
标题翻译：生产复合多元，耐储存反应混合物的方法及其用途
公开(公告)号：US20020039771A1
公开(公告)日：2002-04-04
申请号：US09230070
申请日：1999-03-30
发明人： LARS-ERIK PETERS , PETER BENDZKO
IPC分类号： C12N009/00 , C12N009/96
CPC分类号： C12N9/96
摘要： The invention describes a method and its use for producing complex multienzymatical, storage resistant reaction mixtures for synthesizing, modifying or analyzing polypeptides and optionally nucleic acids characterized in that native or artificial enzymatical, active protein mixtures with reaction buffers, cofactors and substrates are prepared so that they are ready for use and are storage resistant such that only user-specific key components (e.g. mRNS) are missing to start the desired enzymatical reaction(s). In the method a stabilizer is added to the reaction mixtures in the solution which, on the one hand, increases the reacting capacity of the multienzymatical systems and, on the other hand, protects the unstable reaction components from losing their biological activity or their biologically active structure while being made storage resistant and during storage. The reaction mixture is made storage resistant by being easily freeze dried under a vacuum and then durably stored at 4-10null C. (refrigerator temperature). Before use the user has to simply reconstitute the ready prepared reaction mixture by adding the original volume of H2O and start the desired enzymatical reaction(s) by adding the user-specific component(s).
摘要翻译：本发明描述了一种制备用于合成，修饰或分析多肽和任选的核酸的复合多酶，耐贮存的反应混合物的方法及其用途，其特征在于制备天然或人造酶活性蛋白质混合物与反应缓冲液，辅因子和底物，使得它们已经准备好使用并且是耐贮存的，使得仅缺少用户特定的关键组分（例如mRNS）以启动所需的酶反应。在该方法中，将稳定剂加入到溶液中的反应混合物中，一方面增加多酶体系的反应能力，另一方面，保护不稳定的反应组分免于丧失其生物活性或其生物活性结构，同时防止存储和储存期间。通过在真空下容易地冷冻干燥，然后在4-10℃（冰箱温度）下耐久地储存，使反应混合物成为储存的抗性。在使用前，用户必须通过加入原始体积的H 2 O来简单地重建准备好的反应混合物，并通过添加用户特定的组分开始所需的酶反应。

6. 发明申请

US20030022333A1 Long-term shelf preservation by vitrification 审中-公开
标题翻译：通过玻璃化长期保存
公开(公告)号：US20030022333A1
公开(公告)日：2003-01-30
申请号：US10174007
申请日：2002-06-18
发明人： Victor Bronshtein
IPC分类号： C12N009/96 , A01N001/00 , C12N001/04 , C12N005/00 , C12N005/02
CPC分类号： A01N1/0284 , A01N1/02 , A01N1/0221 , A61K35/18 , A61K35/52 , A61K39/00 , C12N1/04 , C12N7/00 , C12N9/00
摘要： A method of shelf preserving biologically active specimens by vitrifying them, i.e., dehydrating them in such a way as to achieve a true glass state at storage temperature by subsequent cooling. The method is founded upon the recognition that to store samples in a true glass state the dehydration temperature of the material to be dehydrated must be higher than the suggested storage temperature. Because the vitrification temperature quickly decreases with increasing water content (for example, pure water vitrifies at Tgnullnull145null C., whereas 80 percent by weight sucrose solution vitrifies at Tgnullnull40null C. and anhydrous sucrose vitrifies at Tgnull60null C.) the sample needs to be strongly dehydrated to increase the Tg above the temperature of storage (Ts). As determined by the inventor, the dehydration temperature should be selected as higher than the suggested storage temperature, and the glass state is subsequently achieved by cooling after dehydration.
摘要翻译：通过将它们玻璃化来保存生物活性样品的方法，即将其脱水以便通过随后的冷却在储存温度下实现真正的玻璃状态。该方法建立在认识到将样品储存在真实玻璃状态下，待脱水材料的脱水温度必须高于建议的储存温度。因为玻璃化温度随着水含量的增加而迅速降低（例如，Tg = -145℃的纯水玻璃化，而在Tg = -40℃下80重量％的蔗糖溶液玻璃化，Tg = 60℃的无水蔗糖玻璃化 ℃），样品需要强力脱水才能使Tg高于储存温度（Ts）。由发明人确定，脱水温度应选择为高于建议的储存温度，然后通过脱水后的冷却来实现玻璃状态。

7. 发明申请

US20020182696A1 Stabilized enzyme compositions 审中-公开
标题翻译：稳定的酶组合物
公开(公告)号：US20020182696A1
公开(公告)日：2002-12-05
申请号：US10132344
申请日：2002-04-25
申请人： COSMOFERM B.V.
发明人： Luppo Edens , Luc Van der Heijden , Albert Jon Vis
IPC分类号： C12N009/96 , A61K038/54
CPC分类号： A61K8/66 , A61K8/345 , A61K8/8158 , A61K47/26 , A61K2800/52
摘要： A stabilized enzyme composition comprising an amount of at least one enzyme, an amount of a polyol for stabilizing the enzyme and an amount of an acrylamide polymer thickening agent, characterized in that as the acrylamide polymer use is made of a null(CH2CR(CONH2)null polymer, in which R is either hydrogen or a methyl group. The concentration of the polyacrylamide in the composition varies from 0.05 to 15 wt %.
摘要翻译：一种稳定的酶组合物，其包含一定量的至少一种酶，一定量的用于稳定酶的多元醇和一定量的丙烯酰胺聚合物增稠剂，其特征在于，作为丙烯酰胺聚合物，使用 - （CH 2 CR（CONH 2） - 聚合物，其中R是氢或甲基。组合物中聚丙烯酰胺的浓度为0.05至15重量％。

8. 发明申请

US20020068860A1 Implantable glucose sensor 有权
标题翻译：植入式葡萄糖传感器
公开(公告)号：US20020068860A1
公开(公告)日：2002-06-06
申请号：US10058453
申请日：2002-01-28
申请人： Implanted Biosystems, Inc.
发明人： Leland C. Clark JR.
IPC分类号： A61B005/00 , C12N009/96 , A61B005/04 , A61B005/05
CPC分类号： A61B5/14865 , C12Q1/002 , C12Q1/006
摘要： The sensitivity of enzyme-based polarographic electrodes to oxygen concentration can be significantly reduced or eliminated by providing an oxygen-reservoir in intimate contact with the oxidative enzyme. This is achieved by making a stabilized emulsion between the enzyme and a compound in which oxygen is extremely soluble. An aqueous glucose oxidase solution is emulsified with a perfluorocarbon liquid, and the resulting emulsion is stabilized by chemically crosslinking the mixture to form a gel. Thin layers of the emulsion are fabricated by spreading a layer of the liquid emulsion before gelation occurs. Additional carrier proteins such as albumin may be added to the enzyme prior to crosslinking to protect enzymatic activity and enhance gel strength. Additional electron transport compounds may be added to further reduce sensitivity to oxygen concentration.
摘要翻译：通过提供与氧化酶紧密接触的氧气储存器，可以显着降低或消除基于酶的极谱法电极对氧浓度的敏感性。这是通过在酶和氧气极易溶解的化合物之间制备稳定的乳液来实现的。葡萄糖氧化酶水溶液用全氟化碳液体乳化，所得乳液通过化学交联混合物形成凝胶来稳定。通过在凝胶化发生之前铺展液体乳液层来制造乳液的薄层。可以在交联之前将另外的载体蛋白如白蛋白加入到酶中以保护酶活性并增强凝胶强度。可以加入另外的电子传输化合物以进一步降低对氧浓度的敏感性。

9. 发明申请

US20030092155A1 Modified phytases 有权
公开(公告)号：US20030092155A1
公开(公告)日：2003-05-15
申请号：US10062848
申请日：2002-02-01
申请人： ROCHE VITAMINS INC.
发明人： Dirk Kostrewa , Luis Pasamontes , Andrea Tomschy , Adolphus van Loon , Kurt Vogel , Markus Wyss
IPC分类号： C12N009/14 , C12N009/16 , C12N009/96 , A61K038/46
CPC分类号： C12N9/16 , A23K20/189 , Y10S435/913 , Y10S435/916 , Y10S435/917
摘要： A process for the production of a modified phytase with a desired property improved over the property of the corresponding unmodified phytase is disclosed, as well as modified phytases, polynucleotides encoding modified phytases, and animal feed including modified phytases.

10. 发明申请

US20020052443A1 Non-antigenic branched polymer conjugates 失效
标题翻译：非抗原支化聚合物共轭物
公开(公告)号：US20020052443A1
公开(公告)日：2002-05-02
申请号：US09545066
申请日：2000-04-07
发明人： Richard B Greenwald , Anthony J Martinez
IPC分类号： C07D493/00 , C07D407/00 , C07D305/00 , A01N043/02 , A61K031/335 , C07D317/70 , C08H001/02 , C08L077/00 , C08L067/00 , C08L071/02 , C08G065/32 , C07K014/00 , C12P021/08 , C07K017/14 , C07K017/00 , C07K016/00 , C07K001/00 , A61K039/385 , C12N009/96 , A61K039/44 , A61K039/42 , A61K039/40 , A61K039/395 , A61K038/54 , C08F283/00 , C08L089/00 , C08G063/91 , C08G063/48 , C08H001/00 , A61K038/16 , A61K038/00 , A01N037/18
CPC分类号： C12N11/08 , A61K47/60 , C07K17/08 , C08G65/329 , C08G65/3322 , C08G65/33396 , C08G65/337 , C08G65/338
摘要： Branched, substantially non-antigenic polymers are disclosed. Conjugates prepared with the polymers and biologically active molecules such as proteins and peptides demonstrate extended circulating life in vivo. Substantially fewer sites on the biologically active material are used as attachment sites. Methods of forming the polymer, conjugating the polymers with biologically active moieties and methods of using the conjugates are also disclosed.
摘要翻译：公开了分支的，基本上非抗原的聚合物。用聚合物和生物活性分子如蛋白质和肽制备的缀合物在体内表现出延长的循环寿命。使用生物活性物质的基本上较少的位点作为附着位点。还公开了形成聚合物，将聚合物与生物活性部分缀合的方法和使用共轭物的方法。

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