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1. 发明专利

JP2014050404A Method and composition to generate dna probes having unique sequence, labeling of dna probes and use of the probes 审中-公开
标题翻译：用于生成具有独特序列的DNA探针的方法和组合，DNA探针的标记和探针的使用
公开(公告)号：JP2014050404A
公开(公告)日：2014-03-20
申请号：JP2013228145
申请日：2013-11-01
申请人： Veridex Llc , ベリデックス・リミテッド・ライアビリティ・カンパニーVeridex,LLC
发明人： CONNELLY MARK CARLE , FOULK BRAD , KAGAN MICHAEL T , SWENNENHUIS JOOST F , TERSTAPPEN LEON W M M , TIBBE ARJAN G J , VERRANT JOHN A
IPC分类号： C12N15/09 , C12Q1/68 , G01N33/53 , G01N37/00
CPC分类号： C12Q1/6886 , C12Q1/34 , C12Q1/6811 , C12Q1/6876 , C12Q2600/106 , C12Q2600/156 , C12Q2600/158 , C12Q2533/101 , C12Q2531/113 , C12Q2521/301 , C12Q2563/131 , C12Q2563/103
摘要： PROBLEM TO BE SOLVED: To provide a method and a composition to obtain DNA probes having a unique sequence, in the field of identification of DNA sequences, genes or chromosomes.SOLUTION: A composition comprises any double stranded DNA containing a unique sequence from which repetitive sequences are eliminated according to the method described in this invention. The invention relates to preservation of cells that are identified after immunomagnetic selection and fluorescent labeling, in order to further investigate the cells of interest. The invention also relates to genetic analysis of cells that are identified after immunomagnetic selection and fluorescent labeling.
摘要翻译：要解决的问题：提供在DNA序列，基因或染色体鉴定领域中获得具有独特序列的DNA探针的方法和组合物。解决方案：组合物包含含有独特序列的任何双链DNA，其中重复根据本发明描述的方法消除序列。本发明涉及在免疫磁性选择和荧光标记之后鉴定的细胞的保存，以便进一步研究感兴趣的细胞。本发明还涉及在免疫磁性选择和荧光标记之后鉴定的细胞的遗传分析。

2. 发明专利

JP2013047688A Method and reagent for quick and efficient isolation of circulating cancer cell 有权
标题翻译：快速有效分离循环癌细胞的方法和试剂
公开(公告)号：JP2013047688A
公开(公告)日：2013-03-07
申请号：JP2012229134
申请日：2012-10-16
申请人： Veridex Llc , ベリデックス・リミテッド・ライアビリティ・カンパニーVeridex,LLC , Board Of Regents The Univ Of Texas System , ボード・オブ・リージエンツ,ザ・ユニバーシテイ・オブ・テキサス・システム
发明人： TERSTAPPEN LEON W M M , RAO GALLA CHANDRA , UHR JONATHAN W , RACILA EMILIAN V , LIBERTI PAUL A
IPC分类号： G01N33/574 , G01N33/58 , B03C1/01 , B82Y5/00 , B82Y25/00 , C12N5/09 , C12Q1/02 , C12Q1/04 , C12Q1/06 , C12Q1/24 , G01N33/48 , G01N33/53 , G01N33/543 , H01F1/00
CPC分类号： B82Y25/00 , B03C1/01 , G01N33/574 , G01N33/57484 , G01N33/57492 , H01F1/0054
摘要： PROBLEM TO BE SOLVED: To provide a reagent, a method and a test kit for easily analyzing and counting tumor cells or any other rare cells isolated from a biological sample, and magnetic particles used for these method and test kit.SOLUTION: There are provided a method for inspecting any incipient cancer in a test object and fractions of peripheral blood in which circulating neoplastic cells are enriched. The method comprises (a) preparing cell fractions from a biological sample obtained from a test object in an immunology magnetic manner, where the sample includes a mixed cell population which has the possibility of containing cancer cells and includes a large amount of disturbing substances, and enriching the cell fractions for cancer cells if the cancer cells exist in the biological sample, and (b) analyzing such enriched fractions for the presence of the cancer cells, where the presence of cancer cells in the sample indicates the existence of incipient cancer in the test object.
摘要翻译：要解决的问题：提供用于容易地分析和计数从生物样品分离的肿瘤细胞或任何其他稀有细胞的试剂，方法和测试试剂以及用于这些方法和测试试剂盒的磁性颗粒。解决方案：提供了一种用于检查测试对象中的任何初期癌症的方法和其中富含循环肿瘤细胞的外周血的部分。该方法包括：（a）以免疫磁性方式从测试对象获得的生物样品中制备细胞级分，其中样品包含可能含有癌细胞并包含大量扰乱物质的混合细胞群，以及如果癌细胞存在于生物样品中，则可浓缩癌细胞的细胞部分，和（b）分析这些富集的部分存在癌细胞，其中样品中存在癌细胞表明存在初期癌症测试对象。版权所有（C）2013，JPO＆INPIT

3. 发明专利

JP2010261963A Methods for enhancing binding interactions between members of specific binding pairs 有权
标题翻译：用于增强特定绑定对成员之间的绑定交互的方法
公开(公告)号：JP2010261963A
公开(公告)日：2010-11-18
申请号：JP2010151171
申请日：2010-07-01
申请人： Veridex Llc , ベリデックス・リミテッド・ライアビリティ・カンパニーVeridex,LLC
发明人： RAO GALLA CHANDRA , TERSTAPPEN LEON W M M , LIBERTI PAUL A
IPC分类号： G01N33/543 , C12Q1/02 , C12Q1/68 , C12Q1/70 , G01N1/36 , G01N33/53 , G01N33/553 , G01N33/566
CPC分类号： G01N33/54333
摘要： PROBLEM TO BE SOLVED: To enhance interaction of a specific binding pair, separation of bioentity (biological entity), and isolation of any tenuous substance from biological fluid.
SOLUTION: In this method, enhanced magnetic load on the target entity is applied to stimulate the above bioseparation, thereby making biochemical and diagnostic analysis of such an isolated target entity easier.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：增强特异性结合对的相互作用，生物实体的分离（生物实体）以及任何脆弱物质与生物流体的分离。解决方案：在这种方法中，应用目标实体的增强磁负荷来刺激上述生物分离，从而使这种孤立目标实体的生物化学和诊断分析更容易。版权所有（C）2011，JPO＆INPIT

4. 发明专利

JP2012175976A Method and composition to generate unique sequence dna probe, labeling of dna probe, and use of the probe 审中-公开
标题翻译：用于生成独特序列DNA探针的方法和组合，DNA探针的标记和探针的使用
公开(公告)号：JP2012175976A
公开(公告)日：2012-09-13
申请号：JP2012099354
申请日：2012-04-25
申请人： Veridex Llc , ベリデックス・リミテッド・ライアビリティ・カンパニーVeridex,LLC
发明人： CONNELLY MARK CARLE , FOULK BRAD , KAGAN MICHAEL T , SWENNENHUIS JOOST F , TERSTAPPEN LEON W M M , TIBBE ARJAN G J , VERRANT JOHN A
IPC分类号： C12Q1/68 , C12M1/34 , C12N15/09 , C12Q1/04 , G01N33/53 , G01N33/543 , G01N37/00
CPC分类号： C12Q1/6886 , C12Q1/34 , C12Q1/6811 , C12Q1/6876 , C12Q2600/106 , C12Q2600/156 , C12Q2600/158 , C12Q2533/101 , C12Q2531/113 , C12Q2521/301 , C12Q2563/131 , C12Q2563/103
摘要： PROBLEM TO BE SOLVED: To provide a method and composition to obtain Unique Sequence DNA probes.SOLUTION: The composition comprises of any double stranded DNA containing Unique Sequences from which the repetitive sequences are eliminated. Rare circulating cells are analyzed by combining phenotypic and genotypic multiparametic analysis of an individually isolated target cell. For the investigation of cells of interest, the cells identified after immunomagnetic selection and fluorescent labeling are preserved for cytogenetic analysis of the cells identified after immunomagnetic selection and fluorescent labeling.
摘要翻译：要解决的问题：提供获得Unique Sequence DNA探针的方法和组合物。解决方案：组合物包含含有重复序列的唯一序列的任何双链DNA。通过组合单独分离的靶细胞的表型和基因型多参数分析来分析稀有的循环细胞。为了研究感兴趣的细胞，在免疫磁选择和荧光标记之后鉴定的细胞被保留用于免疫磁性选择和荧光标记后鉴定的细胞的细胞遗传学分析。版权所有（C）2012，JPO＆INPIT

5. 发明专利

JP2012022002A Method of predicting progression-free and overall survival of metastatic breast cancer patient at each point of follow-up period using circulating tumor cell 审中-公开
标题翻译：使用循环肿瘤细胞在以下各个阶段预测无淋巴结转移癌患者的无进展和全面存活的方法
公开(公告)号：JP2012022002A
公开(公告)日：2012-02-02
申请号：JP2011198211
申请日：2011-09-12
申请人： Veridex Llc , ベリデックス・リミテッド・ライアビリティ・カンパニーVeridex,LLC
发明人： JEFFREY W ALLARD , GERALD V DOYLE , DANIEL F HAYES , MICHAEL CRAIG MILLER , TERSTAPPEN LEON W M M
IPC分类号： G01N33/53 , C12Q1/04 , G01N33/48 , G01N33/543
摘要： PROBLEM TO BE SOLVED: To provide a method of testing cancer having prognostic utility in predicting time to disease progression, overall survival, and response to therapy in a patient with MBC(metastatic breast cancer) based on the presence and number of CTC(circulating tumor cell)s.SOLUTION: Cell Spotter(R) System is used to count CTCs in blood. The system immunomagnetically concentrates epithelial cells, fluorescently labels the cells, identifies and quantifies CTCs. The absolute number of CTCs detected in the peripheral blood tumor load is, in part, a factor in the prediction of survival, time to progression, and response to therapy. The average survival time of patients depends on the threshold number of 5 CTCs per 7.5 ml of blood. The detection of CTCs in metastatic cancer represents a novel diagnostic factor in a patient with metastatic cancer, suggests the biological role of the presence of tumor cells in blood, and indicates that the detection of CTCs could be considered an appropriate surrogate marker for future therapeutic clinical trials.
摘要翻译：要解决的问题：提供一种测试癌症的方法，其具有预测基于CTC的存在和数量的患有MBC（转移性乳腺癌）的患者的疾病进展时间，总体存活和对治疗的反应的预后效果（循环肿瘤细胞）。
解决方案：Cell Spotter（R）系统用于计算血液中的CTC。系统免疫磁性浓缩上皮细胞，荧光标记细胞，识别和定量CTC。在外周血肿瘤负荷中检测到的CTC的绝对数量部分是预测存活时间，进展时间和对治疗反应的因素。患者的平均生存时间取决于每7.5毫升血液的5个CTC的阈值数。转移癌中CTC的检测代表了转移性癌症患者的一种新型诊断因素，表明肿瘤细胞存在于血液中的生物学作用，并表明CTCs的检测可以被认为是未来治疗临床的适当替代标记试验。版权所有（C）2012，JPO＆INPIT

6. 发明专利

JP2010019850A Magnetic separator and method thereof 有权
标题翻译：磁选分离器及其方法
公开(公告)号：JP2010019850A
公开(公告)日：2010-01-28
申请号：JP2009220205
申请日：2009-09-25
申请人： Veridex Llc , ベリデックス・リミテッド・ライアビリティ・カンパニーVeridex,LLC
发明人： TERSTAPPEN LEON W M M
IPC分类号： G01N33/483 , G01N33/48
摘要： PROBLEM TO BE SOLVED: To provide an improved apparatus for implementing qualitative and quantitative analyses of a microscopic biological sample, and a method thereof. SOLUTION: The apparatus and method is for separating, immobilizing, and quantifying a biological substance from within a fluid medium. The biological substance is observed by employing a vessel 6 incorporating a chamber, the vessel including a transparent collection wall 5. A magnetic capture structure with a high internal gradient is on the transparent collection wall 5 and magnets 3 produce an externally-applied force for transporting a magnetically responsive material toward this transparent collection wall 5. This magnetic capture structure includes a conductor means supported on the transparent collection wall 5 in order to enable electrical manipulation of the biological substance. This chamber includes one compartment or a plurality of compartments with different heights. The chamber may have a porous wall. The present invention is also useful in conducting a quantitative analysis and sample preparation in conjunction with an automated cell enumeration technique. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供一种用于实现微观生物样品的定性和定量分析的改进装置及其方法。解决方案：该装置和方法用于从流体介质中分离，固定和定量生物物质。通过使用结合有腔室的容器6观察生物物质，容器包括透明收集壁5.具有高内部梯度的磁捕获结构在透明收集壁5上，并且磁体3产生用于运送的外部施加的力磁性响应材料朝向该透明收集壁5.该磁捕获结构包括支撑在透明收集壁5上的导体装置，以便能够对生物物质进行电操作。该室包括具有不同高度的一个隔室或多个室。腔室可以具有多孔壁。本发明还可用于结合自动细胞计数技术进行定量分析和样品制备。版权所有（C）2010，JPO＆INPIT

7. 发明专利

JP2009192539A Method and apparatus for imaging target components in biological sample using permanent magnet 有权
标题翻译：使用永久磁铁在生物样品中成像目标组件的方法和装置
公开(公告)号：JP2009192539A
公开(公告)日：2009-08-27
申请号：JP2009032033
申请日：2009-02-13
申请人： Veridex Llc , ベリデックス・エルエルシーVeridex,LLC
发明人： TIBBE ARJAN G J , TERSTAPPEN LEON W M M
IPC分类号： G01N33/53 , G01N21/64 , G01N33/553
CPC分类号： G01N33/582 , G01N33/54326 , G01N33/56972 , G01N2333/70514 , G01N2446/00
摘要： PROBLEM TO BE SOLVED: To provide a system for enumeration of cells in fluids by image cytometry for assessment of target populations such as leukocyte partial group in different bodily fluids, or bacterial contamination in environmental samples, food products and bodily fluids.
SOLUTION: Fluorescently labeled target cells are linked to magnetic particles or beads. In one embodiment, a small permanent magnet is inserted directly into the chamber containing the labeled cells. The magnets are coated with PDMS silicone rubber to provide a smooth and even surface which allows imaging on a single focal plane. The magnet is removed from the sample and illuminated with fluorescent light emitted by the target cells captured by a CCD camera. In another embodiment, a floater having a permanent magnet makes the target cells array along a single imaging plane within the sample solution. An image analysis can be performed with a novel algorithm for measuring cells on the surface reflecting the target cell concentration of the original sample.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供一种用于通过图像细胞计数来计量流体中的细胞的系统，以评估靶群体，例如不同体液中的白细胞部分组，或环境样品，食品和体液中的细菌污染。荧光标记的靶细胞与磁性颗粒或珠子连接。在一个实施例中，将小永久磁铁直接插入含有标记细胞的腔室中。这些磁体用PDMS硅橡胶涂覆，以提供平滑和均匀的表面，允许在单个焦平面上进行成像。从样品中除去磁体，并用由CCD照相机俘获的靶细胞发射的荧光照射。在另一个实施例中，具有永磁体的浮子使得靶单元沿着样品溶液内的单个成像平面排列。可以使用用于测量反映原始样品的靶细胞浓度的表面上的细胞的新颖算法进行图像分析。版权所有（C）2009，JPO＆INPIT

8. 发明申请

WO2009143149A3 IMPROVED IMAGING OF IMMUNOMAGNETICALLY ENRICHED RARE CELLS 审中-公开
标题翻译：改善免疫细胞增殖的成熟细胞
公开(公告)号：WO2009143149A3
公开(公告)日：2010-01-14
申请号：PCT/US2009044510
申请日：2009-05-19
申请人： VERIDEX LLC , SCHOLTENS TYCHO M , SCHREUDER FREDERIK , GREVE JAN , TIBBE ARJAN , TERSTAPPEN LEON W M M
发明人： SCHOLTENS TYCHO M , SCHREUDER FREDERIK , GREVE JAN , TIBBE ARJAN , TERSTAPPEN LEON W M M
IPC分类号： B03C1/033
CPC分类号： G01N33/54326
摘要： A method for removing excess unbound ferrofluid and imaging immunomagnetically enriched circulating tumor cells is provided. A vessels having a preformed grooves in the viewing surface is optimally designed for cell alignment and imaging. After separating the unbound particles by centrifugation, an externally-applied force is applied to transport magnetically responsive particle-CTC complex toward the transparent collection wall. The grooved inner surface of the viewing face of the chamber provide uniform distribution of the particles for easy imaging. The invention is also useful in conducting quantitative analysis and sample preparation in conjunction with automated cell enumeration techniques as in quantitative analysis of CTC in disease.
摘要翻译：提供了一种去除过量未结合的铁磁流体和成像免疫磁性富集的循环肿瘤细胞的方法。在观察表面中具有预成形凹槽的容器被最佳设计用于细胞对准和成像。通过离心分离未结合的颗粒后，施加外部施加的力以将磁响应颗粒-CTC复合物输送到透明收集壁。室的观察面的带槽的内表面提供了均匀分布的颗粒以便于成像。本发明还可用于与自动细胞计数技术结合进行定量分析和样品制备，如在疾病中CTC的定量分析中。

9. 发明专利

CA2759764C METHODS AND ALGORITHMS FOR CELL ENUMERATION IN A LOW-COST CYTOMETER 未知
公开(公告)号：CA2759764C
公开(公告)日：2017-06-13
申请号：CA2759764
申请日：2003-02-14
申请人： VERIDEX LLC
发明人： DROOG ERIK , TIBBE ARJAN , GREVE JAN , GOHEL DHANESH , TERSTAPPEN LEON W M M
IPC分类号： G01N15/10 , G01N21/64 , C12M1/34 , C12Q1/06 , G01N15/00 , G01N15/14 , G01N21/78 , G01N33/53 , G01N33/543 , G01N33/545 , G01N33/567
摘要： The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote.

10. 发明专利

BRPI0910038A2 método para detecção de igf1r/chr 15 em células tumorais circulantes com o uso de fish (hibridização in situ fluorescente) 未知
公开(公告)号：BRPI0910038A2
公开(公告)日：2015-12-29
申请号：BRPI0910038
申请日：2009-03-25
申请人： VERIDEX LLC
发明人： FOULK BRAD , TERSTAPPEN LEON W M M
IPC分类号： C12Q1/68

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