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1. 发明授权

US5416015A Isolated genes encoding peptidyl prolyl-cis.trans-isomerase and plasmids comprising said genes 失效
标题翻译：编码肽基脯氨酰基 - 顺反异构酶的分离基因和包含所述基因的质粒
公开(公告)号：US5416015A
公开(公告)日：1995-05-16
申请号：US89850
申请日：1993-07-09
申请人： Toshiya Hayano , Setsuko Katou , Noboru Maki , Nobuhiro Takahashi , Masanori Suzuki
发明人： Toshiya Hayano , Setsuko Katou , Noboru Maki , Nobuhiro Takahashi , Masanori Suzuki
IPC分类号： C12N9/90 , C12N15/61 , C12N15/63 , C12N15/70
CPC分类号： C12N9/90
摘要： A yeast PPIase characterized by possessing following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro stands for L-proline), (2) exhibiting a single molecular weight of about 17,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 6.2 in the isoelectric focusing, and (4) inhibited by CsA;an E. coli PPIase-.beta. characterized by possessing the following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro for L-proline), (2) exhibiting a single molecular weight of about 20,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 5.0 in the isoelectric focusing, and (4) no being inhibited by CsA;an E. coli PPIase-.alpha. characterized by possessing the following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro for L-proline), (2) exhibiting a single molecular weight of about 22,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 9.7 in the isoelectric focusing, and (4) not being inhibited by CsA.
摘要翻译：特征在于具有以下性质的酵母PPI酶：（1）作用于Xaa-Pro键（Xaa代表任何氨基酸，Pro代表L-脯氨酸），（2）表现出约17,000道尔顿单分子量在十二烷基硫酸钠 - 聚丙烯酰胺浓度梯度凝胶电泳中，（3）在等电聚焦中表现出约6.2的单一等电点，（4）被CsA抑制; 特征在于具有以下性质的大肠杆菌PPI酶-β：（1）作用于Xaa-Pro（Xaa代表任何氨基酸，Pro-L-脯氨酸，Xaa代表任意氨基酸，Pro表示单分子量）在十二烷基硫酸钠 - 聚丙烯酰胺浓度梯度凝胶电泳中为约20,000道尔顿，（3）在等电聚焦中呈现约5.0的单等电点，（4）不被CsA抑制; 其特征在于具有以下性质：（1）作用于Xaa-Pro键（Xaa代表任何氨基酸，Pro为L-脯氨酸），（2）表现出单分子量在十二烷基硫酸钠 - 聚丙烯酰胺浓度梯度凝胶电泳中约为22,000道尔顿，（3）在等电聚焦中显示出约9.7的单一等电点，（4）不被CsA抑制。

2. 发明授权

US5284762A Peptidyl prolyl-cis.trans-isomerase 失效
标题翻译：肽基脯氨酰 - 顺反异构酶
公开(公告)号：US5284762A
公开(公告)日：1994-02-08
申请号：US555944
申请日：1990-07-19
申请人： Toshiya Hayano , Setsuko Katou , Noboru Maki , Nobuhiro Takahashi , Masanori Suzuki
发明人： Toshiya Hayano , Setsuko Katou , Noboru Maki , Nobuhiro Takahashi , Masanori Suzuki
IPC分类号： C12N9/90 , C12N15/61 , C12N15/70
CPC分类号： C12N9/90
摘要： A yeast PPIase characterized by possessing the following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro stands for L-proline), (2) exhibiting a single molecular weight of about 17,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 6.2 in the isoelectric focusing, and (4) inhibited by CsA;an E. coli PPIase-.beta. characterized by possessing the following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro for L-proline), (2) exhibiting a single molecular weight of about 20,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 5.0 in the isoelectric focusing, and (4) no being inhibited by CsA;an E. coli PPIase-.alpha. characterized by possessing the following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro for L-proline), (2) exhibiting a single molecular weight of about 22,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 9.7 in the isoelectric focusing, and (4) not being inhibited by CsA.
摘要翻译：特征在于具有以下特性的酵母PPI酶：（1）作用于Xaa-Pro键（Xaa代表任何氨基酸，Pro代表L-脯氨酸），（2）表现出单一分子量约17,000 十二烷基硫酸钠 - 聚丙烯酰胺浓度梯度凝胶电泳中的道尔顿，（3）在等电聚焦中显示约6.2的单一等电点，和（4）被CsA抑制; 特征在于具有以下特性的大肠杆菌PPIase-（β）：（1）作用于Xaa-Pro键（Xaa代表任何氨基酸，Pro代表L-脯氨酸），（2）表现出单一的在十二烷基硫酸钠 - 聚丙烯酰胺浓度梯度凝胶电泳中分子量约20,000道尔顿，（3）在等电聚焦中呈现约5.0的单一等电点，（4）不被CsA抑制; 特征在于具有以下性质的大肠杆菌PPIase-（α）：（1）作用于Xaa-Pro键（Xaa代表任何氨基酸，Pro代表L-脯氨酸），（2）表现出单一的在十二烷基硫酸钠 - 聚丙烯酰胺浓度梯度凝胶电泳中分子量约22,000道尔顿，（3）在等电聚焦中显示约9.7的单一等电点，（4）不被CsA抑制。

3. 发明授权

US5759802A Production of human serum alubumin A 失效
标题翻译：生产人血清aluminumin A
公开(公告)号：US5759802A
公开(公告)日：1998-06-02
申请号：US417429
申请日：1989-10-05
申请人： Noboru Maki , Shintaro Yagi , Kazuya Watanabe , Masanori Suzuki
发明人： Noboru Maki , Shintaro Yagi , Kazuya Watanabe , Masanori Suzuki
IPC分类号： C07K14/765 , C12N1/19 , C12N15/14 , C12N15/62 , C12N15/81 , C12P21/06 , C12N1/14 , C12N15/00 , C12P21/04
CPC分类号： C07K14/765 , C12N15/625 , C12N15/81 , C07K2319/02 , C07K2319/036
摘要： A process for the production of human serum albumin A (HSA) characterized by culturing host cells transformed with the plasmids containing a leader DNA sequence and a cDNA coding for mature HSA to produce and secrete mature HSA, and recovering the mature HSA. As the leader HSA sequence, a cDNA coding for the prepro sequence of HSA, a synthetic DNA coding for the prepro sequence of HSA, by the codons frequently used in a selected host, a DNA coding for a chimeric leader peptide, a DNA coding for MF.alpha.1 prepro sequence, or a DNA coding for PH05 signal peptide, is used.
摘要翻译：一种生产人血清白蛋白A（HSA）的方法，其特征在于培养用含有前导DNA序列的质粒转化的宿主细胞和编码成熟HSA的cDNA，以产生和分泌成熟HSA，并回收成熟HSA。作为前导HSA序列，编码HSA的前序列的cDNA，编码HSA的前序列的合成的DNA，经选择的宿主中常用的密码子，编码嵌合前导肽的DNA，编码使用MFα1前原序列或编码PH05信号肽的DNA。

4. 发明授权

US08679762B2 Method of detecting hepatitis B virus s antigen 有权
标题翻译：检测乙型肝炎病毒抗原的方法
公开(公告)号：US08679762B2
公开(公告)日：2014-03-25
申请号：US11663517
申请日：2005-09-21
申请人： Noboru Maki , Yasuyuki Fukuda , Tatsuji Kimura , Yoko Oda , Chiharu Ohue , Osamu Kusano
发明人： Noboru Maki , Yasuyuki Fukuda , Tatsuji Kimura , Yoko Oda , Chiharu Ohue , Osamu Kusano
IPC分类号： C12Q1/70 , C07H21/04 , A61K39/395
CPC分类号： G01N33/5761 , C07K14/005 , C07K16/082 , C12N2730/10122
摘要： [PROBLEMS] To provide a probe useful in the detection of HBV or HBs antigen by which an escape mutant of hepatitis B virus (HBV) possibly occurring in a specimen can be detected; and a method of using the same.[MEANS FOR SOLVING PROBLEMS] A probe capable of recognizing an epitope located on a peptide comprising the amino acid sequence of SEQ ID NO:1; and a method of detecting hepatitis B virus or hepatitis B virus s antigen by using this probe.
摘要翻译： [问题]提供可用于检测HBV或HBs抗原的探针，通过该探针可以检测可能发生在样品中的乙型肝炎病毒（HBV）的逃逸突变体; 及其使用方法。解决问题的手段能够识别位于包含SEQ ID NO：1的氨基酸序列的肽的表位的探针; 以及通过使用该探针检测乙型肝炎病毒或乙型肝炎病毒抗原的方法。

5. 发明申请

US20070264646A1 Hbv Particles Containing Hbv-Rna 审中-公开
标题翻译：含有Hbv-Rna的Hbv颗粒
公开(公告)号：US20070264646A1
公开(公告)日：2007-11-15
申请号：US11630254
申请日：2004-06-22
申请人： Noboru Maki , Tatsuji Kimura , Akihiro Matsumoto , Akinori Rokuhara , Eiji Tanaka , Kendo Kiyosawa
发明人： Noboru Maki , Tatsuji Kimura , Akihiro Matsumoto , Akinori Rokuhara , Eiji Tanaka , Kendo Kiyosawa
IPC分类号： C12Q1/68 , C07H21/04 , C12N7/00
CPC分类号： C12N7/00 , C12N2730/10123 , C12Q1/706
摘要： Hepatitis B virus (HBV) particles that contain HBV-RNA but do not contain HBV-DNA in a body fluid sample; A method of measuring the particles according to claim 1 by measuring HBV-RNA; A method of judging the therapeutic effect of the treatment of HBV infections by a drug having an effect of inhibiting RNA-dependent DNA polymerase, which method comprises performing the measurement on a body fluid of a patient who received said treatment; A method of predicting the risk of developing hepatic carcinoma or selecting risk groups of developing hepatic carcinoma by the above measurement.
摘要翻译：在体液样品中含有HBV-RNA但不含有HBV-DNA的乙型肝炎病毒（HBV）颗粒; 一种通过测量HBV-RNA测量根据权利要求1的颗粒的方法; 一种判断通过具有抑制RNA依赖性DNA聚合酶作用的药物治疗HBV感染的治疗效果的方法，该方法包括对接受所述治疗的患者的体液进行测定; 通过上述测量预测肝癌发展风险或选择发展成肝癌风险组的方法。

6. 发明授权

US5662906A Non-a non-b hepatitis-specific antigen and its use in hepatitis diagnosis 失效
标题翻译：非乙型肝炎特异性抗原及其在肝炎诊断中的应用
公开(公告)号：US5662906A
公开(公告)日：1997-09-02
申请号：US449093
申请日：1995-05-24
申请人： Noboru Maki , Kenjiro Yamaguchi , Ayumi Toyoshima , Michinori Kohara
发明人： Noboru Maki , Kenjiro Yamaguchi , Ayumi Toyoshima , Michinori Kohara
IPC分类号： C07K14/18 , C12N15/40 , A61K39/29
CPC分类号： C07K14/005 , C12N2770/24222
摘要： This invention relates to a DNA fragment comprising a base sequence encoding a non-A non-B hepatitis-specific antigen polypeptide, said base sequence being obtained using genetic engineering techniques from non-A non-B hepatitis virus RNA which is isolated directly from blood plasma from non-A non-B hepatitis patients, to an expression vector and a transformant for use in the expression of the DNA fragment, to a single strand DNA sequence for PCR primer, and to use of said polypeptide and said single strand DNA sequence in the detection of the non-A non-B hepatitis virus. The recombinant polypeptide and the single strand DNA sequence for PCR primer make it possible to detect the non-A non-B hepatitis virus with extremely high accuracy.
摘要翻译：本发明涉及包含编码非A非乙型肝炎特异性抗原多肽的碱基序列的DNA片段，所述碱基序列使用从直接从血液中分离的非A非乙型肝炎病毒RNA的遗传工程技术获得来自非A非B型肝炎患者的血浆，用于表达DNA片段的表达载体和转化体，用于PCR引物的单链DNA序列，以及使用所述多肽和所述单链DNA序列在非A非乙型肝炎病毒的检测中。用于PCR引物的重组多肽和单链DNA序列使得可以以非常高的精度检测非A非B型肝炎病毒。

7. 发明授权

US08981073B2 Hepatitis C virus gene 有权
标题翻译：丙型肝炎病毒基因
公开(公告)号：US08981073B2
公开(公告)日：2015-03-17
申请号：US13823422
申请日：2011-10-07
申请人： Kenichi Mori , Noboru Maki , Hiromi Fukai
发明人： Kenichi Mori , Noboru Maki , Hiromi Fukai
IPC分类号： C07H21/04 , A01N63/02 , C12N7/00 , C07H21/02 , C07K14/005
CPC分类号： C07H21/02 , C07K14/005 , C12N2770/24222
摘要： Disclosed are an HCV gene having higher replication efficiency and higher reinfection efficiency than the known HCV gene of genotype 1b, an RNA replicon having this gene, a cell infected with this RNA replicon, which cell allows replication of HCV, and an HCV particle. The hepatitis C virus gene encodes an amino acid sequence wherein the 979th amino acid is threonine; the 1804th amino acid is leucine; and the 1966th amino acid is lysine. An HCV gene which can propagate in vitro and has higher replication efficiency and higher reinfection efficiency than the known HCV gene of genotype 1b was provided.
摘要翻译：公开了具有比基因型1b的已知HCV基因，具有该基因的RNA复制子，感染了该RNA复制子的细胞，该细胞允许HCV复制和HCV颗粒更高的复制效率和更高的再感染效率的HCV基因。丙型肝炎病毒基因编码氨基酸序列，其中第979位氨基酸是苏氨酸; 第1804位氨基酸为亮氨酸; 而第1966位氨基酸是赖氨酸。提供了可以在体外繁殖并且具有比已知的基因型1b的HCV基因更高的复制效率和更高的再感染效率的HCV基因。

8. 发明授权

US08741607B2 HCV/GBV-B chimeric virus 失效
标题翻译： HCV / GBV-B嵌合病毒
公开(公告)号：US08741607B2
公开(公告)日：2014-06-03
申请号：US13054356
申请日：2009-07-15
申请人： Noboru Maki , Kenichi Mori , Hiromi Fukai
发明人： Noboru Maki , Kenichi Mori , Hiromi Fukai
IPC分类号： C12N15/09 , C12N5/10 , C12N7/00 , C07K14/18
CPC分类号： C07K14/005 , C12N7/00 , C12N2770/24221 , C12N2770/24222 , C12N2770/24243
摘要： An HCV/GBV-B chimeric virus which maintains the replication function of HCV and is capable of infecting tamarin is disclosed in order to construct an HCV animal model which can be used as a development or evaluation system for therapeutic agents for HCV. The HCV/GBV-B chimeric RNA comprises an RNA of hepatitis C virus and an RNA of GB virus-B, wherein the RNA of hepatitis C virus comprises an RNA encoding leucine at the 1804th position and lysine at the 1966th position in the amino acid sequence of the polyprotein of hepatitis C virus.
摘要翻译：公开了保持HCV的复制功能并能够感染t am素的HCV / GBV-B嵌合病毒，以构建可用作HCV治疗剂的开发或评估系统的HCV动物模型。 HCV / GBV-B嵌合RNA包含丙型肝炎病毒的RNA和GB病毒B的RNA，其中丙型肝炎病毒的RNA包含编码第1804位亮氨酸的RNA，氨基酸中第1966位的赖氨酸丙型肝炎病毒多蛋白序列。

9. 发明申请

US20090017443A1 Method for Detection of Hepatitus B Virus 审中-公开
标题翻译：乙肝病毒检测方法
公开(公告)号：US20090017443A1
公开(公告)日：2009-01-15
申请号：US11596663
申请日：2005-05-19
申请人： Chiharu Ohue , Noboru Maki , Tatsuji Kimura
发明人： Chiharu Ohue , Noboru Maki , Tatsuji Kimura
IPC分类号： C12Q1/70
CPC分类号： G01N33/5761
摘要： To provide a method for detection or quantification of hepatitis B virus (HBV) antigens in serum and a simple and highly user-friendly method for sample treatment for use in the detection or quantification thereof. The method for treatment of a sample containing hepatitis B virus (HBV) is characterized in that release of HBV antigens and disruption of antibodies that bind to HBV antigens are carried out by treating a sample containing HBV with a treatment agent containing (1) an acidifying agent and (2) a protein denaturant or an amphoteric surfactant or cationic surfactant having an alkyl group and a tertiary amine or a quaternary ammonium salt within a molecule.
摘要翻译：提供血清中乙型肝炎病毒（HBV）抗原的检测或定量的方法，以及用于检测或定量的用于样品处理的简单且高度用户友好的方法。用于治疗含有乙型肝炎病毒（HBV）的样品的方法的特征在于通过用含有（1）酸化的处理剂处理含有HBV的样品来进行HBV抗原的释放和与HBV抗原结合的抗体的破坏试剂和（2）分子内具有烷基和叔胺或季铵盐的蛋白质变性剂或两性表面活性剂或阳离子表面活性剂。

10. 发明授权

US07323331B2 Method for detecting or assaying HBV 有权
标题翻译： HBV检测方法
公开(公告)号：US07323331B2
公开(公告)日：2008-01-29
申请号：US10343324
申请日：2001-08-10
申请人： Noboru Maki , Tatsuji Kimura , Yoko Oda , Shintaro Yagi
发明人： Noboru Maki , Tatsuji Kimura , Yoko Oda , Shintaro Yagi
IPC分类号： C12N5/00 , C12N5/06 , C12N5/12 , C12N5/18 , C12N5/20 , C12Q1/00 , C12Q1/70 , G01N33/53 , C07K16/08
CPC分类号： G01N33/5762 , C07K16/082
摘要： A Method for detecting hepatitis B virus as well as a kit and a reagent therefor, comprising using a phosphate buffer that specifically binds to hepatitis B virus, for example a monoclonal antibody that specifically binds to a specific site of core-related protein of hepatitis B virus.
摘要翻译：一种检测乙型肝炎病毒的方法以及试剂盒及其试剂，其包括使用特异性结合乙型肝炎病毒的磷酸盐缓冲液，例如特异性结合乙型肝炎核心相关蛋白质的特定位点的单克隆抗体病毒。

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