会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 7. 发明申请
    • Detection Method of Dna Amplification Using Probe Labeled With Intercalating Dye
    • 用插入染料标记的探针的Dna扩增检测方法
    • US20080220415A1
    • 2008-09-11
    • US10593900
    • 2005-03-25
    • Han Oh ParkHyun-Bae KimSung-Min Chi
    • Han Oh ParkHyun-Bae KimSung-Min Chi
    • C12Q1/68
    • C12Q1/6844C12Q1/686C12Q2563/173C12Q2563/107C12Q2561/113
    • The present invention relates to a detection method of nucleic acid amplification using probe labeled with intercalating dye. More particularly, the present invention is directed to a real-time detection method of nucleic acid amplification, comprising the steps of i) producing an aqueous buffer which contains a nucleic acid, a pair of primers for amplification of said nucleic acid, a fluorescent probe wherein a fluorescent dye of which intensity of fluorescence is varied when the dye is intercalated into a double-stranded nucleic acid, is connected with an oligonucleotide of which base sequence is complementary with at least a part of said nucleic acid, four (4) kinds of nucleotides and DNA polymerase; ii) denaturing said doublestranded nucleic acid into single strands by heating the aqueous buffer prepared in step i) up to 931 C to 96 C; iii) annealing said pair of primers with said single strand by cooling the solution obtained in step ii) up to 50 C. to 571 C; iv) replicating said single-stranded nucleic acid by heating the solution obtained from step iii) up to 701 C to 74° C.; v) denaturing said replicated nucleic add into single strands by heating the solution obtained in step iv) up to 931 C to 961 C; vi) annealing said fluorescent probe with said single-stranded nucleic acid by cooling the solution obtained in step v up to 501 C to 57 C; vii) measuring an intensity of the fluorescence emitted from the solution obtained in step vi); and viii) repeating more than one steps iv) through vii).
    • 本发明涉及使用用插层染料标记的探针的核酸扩增检测方法。 更具体地,本发明涉及核酸扩增的实时检测方法,包括以下步骤:i)产生含有核酸的水性缓冲液,用于扩增所述核酸的一对引物,荧光探针 其中当染料插入双链核酸时荧光强度变化的荧光染料与其碱基序列与所述核酸的至少一部分互补的寡核苷酸连接,四(4)种 的核苷酸和DNA聚合酶; ii)通过加热步骤i)中制备的含水缓冲液至931℃至96℃,将所述双链核酸变性为单链; iii)通过将步骤ii)中获得的溶液冷却至50℃至571℃,用所述单链退火所述一对引物; iv)通过将从步骤iii)获得的溶液加热至701℃至74℃来复制所述单链核酸; v)通过将步骤iv)中获得的溶液加热至931℃至961℃,将所述复制的核酸添加到单链中; vi)通过将步骤v中获得的溶液冷却至501℃至57℃,用所述单链核酸退火所述荧光探针; vii)测量从步骤vi)中获得的溶液发射的荧光的强度; 和viii)重复多于一个步骤iv)至vii)。