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1. 发明授权

US08841118B2 Combined detection instrument for culture specimen containers and instrument for identification and/or characterization of a microbial agent in a sample 有权
标题翻译：用于培养样品容器的组合检测仪器和用于鉴定和/或表征样品中的微生物剂的仪器
公开(公告)号：US08841118B2
公开(公告)日：2014-09-23
申请号：US12800467
申请日：2010-05-14
申请人： Ronnie J. Robinson , Mark S. Wilson , Christopher S. Ronsick , John D. Walsh , Jones M. Hyman , Thurman C. Thorpe , Bradford G. Clay
发明人： Ronnie J. Robinson , Mark S. Wilson , Christopher S. Ronsick , John D. Walsh , Jones M. Hyman , Thurman C. Thorpe , Bradford G. Clay
IPC分类号： C12M1/00 , C12M3/00 , G01N35/00 , C12M1/34 , C12Q1/04 , G01N35/04
CPC分类号： C12Q1/04 , C12M41/36 , C12Q1/02 , G01N35/00603 , G01N35/0099 , G01N35/026 , G01N2035/00148 , G01N2035/0406 , G01N2035/0453 , G01N2035/0465
摘要： A detection instrument determines whether a specimen container (e.g., blood culture bottle) is positive for presence of microbial agent growth therein. When the container is deemed positive it is made available (e.g, transferred or exposed to) to an automated instrument performing identification and/or characterization of the microbial agent. The identification and/or characterization instrument removes a portion of the sample from the specimen container and places it into a disposable separation and concentration device. The microbial agent is concentrated via optional selective lysis of non-microbial agent cellular material which may be present and centrifugation. A reading module reads the concentrated microbial agent using spectroscopic methods, e.g., measurements of intrinsic fluorescence. Such interrogation may occur while the microbial agent remains concentrated in the disposable device.
摘要翻译：检测仪器确定样品容器（例如血培养瓶）是否对其中存在微生物剂生长是阳性的。当容器被认为是正的时，它被提供（例如，转移或暴露于）到执行微生物剂的鉴定和/或表征的自动化仪器。识别和/或表征仪器将样品的一部分从样品容器中取出并将其放入一次性分离和浓缩装置中。微生物剂通过选择性裂解可能存在的和非离子的非微生物剂细胞材料而浓缩。阅读模块使用光谱方法读取浓缩的微生物剂，例如固有荧光的测量。当微生物剂保持集中在一次性装置中时，可能发生这种询问。

2. 发明授权

US08512975B2 Method for detection and characterization of a microorganism in a sample using time dependent spectroscopic measurements 有权
标题翻译：使用时间依赖光谱测量法检测和表征样品中微生物的方法
公开(公告)号：US08512975B2
公开(公告)日：2013-08-20
申请号：US12460607
申请日：2009-07-22
申请人： John D. Walsh , Jones M. Hyman , Bradford G. Clay , Thurman C. Thorpe
发明人： John D. Walsh , Jones M. Hyman , Bradford G. Clay , Thurman C. Thorpe
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , C12Q1/10 , G01N21/31 , G01N21/47 , G01N21/64 , G01N21/6486 , G01N2021/6419 , G01N2021/6421
摘要： The present invention provides a method for detecting, and characterizing a microorganism present in a blood culture medium whereby the method may be accomplished utilizing a time-dependent spectroscopic technique to obtain at least two measurements directly of a blood culture medium and correlating said measurements for the detection and characterization of a microorganism, that may be present in the blood culture.
摘要翻译：本发明提供了一种用于检测和表征存在于血液培养基中的微生物的方法，由此可以使用时间依赖性光谱技术来完成该方法，以直接获得血液培养基的至少两次测量，并将所述测量结果与可能存在于血液培养物中的微生物的检测和表征。

3. 发明申请

US20100068755A1 Method and system for detection and/or characterization of a biological particle in a sample 有权
标题翻译：用于样品中生物颗粒的检测和/或表征的方法和系统
公开(公告)号：US20100068755A1
公开(公告)日：2010-03-18
申请号：US12460607
申请日：2009-07-22
申请人： John D. Walsh , Jones M. Hyman , Bradford G. Clay , Thurman C. Thorpe
发明人： John D. Walsh , Jones M. Hyman , Bradford G. Clay , Thurman C. Thorpe
IPC分类号： C12Q1/04 , C12M1/34
CPC分类号： C12Q1/04 , C12Q1/10 , G01N21/31 , G01N21/47 , G01N21/64 , G01N21/6486 , G01N2021/6419 , G01N2021/6421
摘要： The present invention provides a method and system for monitoring, detecting, and/or characterizing a biological particle that may be present in a sample whereby the method may be accomplished non-invasively by utilizing a time-dependent spectroscopic technique to obtain at least two measurements of a growth composition comprising a sample and correlating said measurements for the detection and/or characterization of a biological particle, that may be present in the sample.
摘要翻译：本发明提供了一种用于监测，检测和/或表征可能存在于样品中的生物颗粒的方法和系统，其中所述方法可以非侵入地通过利用时间依赖的光谱技术来获得至少两个测量的生长组合物，其包含样品并将所述测量结果与可能存在于样品中的生物颗粒的检测和/或表征相关联。

4. 发明申请

US20140335558A1 Method for the Characterization of Microorganisms on Solid or Semi-Solid Media 有权
标题翻译：固体或半固体培养基上微生物表征的方法
公开(公告)号：US20140335558A1
公开(公告)日：2014-11-13
申请号：US14444708
申请日：2014-07-28
申请人： Jones M. Hyman , John D. Walsh , Thurman C. Thorpe
发明人： Jones M. Hyman , John D. Walsh , Thurman C. Thorpe
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04
摘要： The present invention relates to methods and systems for scanning, detecting, and monitoring microorganisms on solid or semi-solid media using intrinsic fluorescence (IF) measurements. The methods are further directed to detection, characterization and/or identification of microorganisms on a solid or semi-solid media using intrinsic fluorescence (IF) measurements that are characteristic of said microorganisms.
摘要翻译：本发明涉及使用固有荧光（IF）测量在固体或半固体培养基上扫描，检测和监测微生物的方法和系统。所述方法进一步涉及使用所述微生物特征的固有荧光（IF）测量法在固体或半固体培养基上检测，表征和/或鉴定微生物。

5. 发明授权

US09822389B2 Method for the characterization of microorganisms on solid or semi-solid media 有权
公开(公告)号：US09822389B2
公开(公告)日：2017-11-21
申请号：US14444708
申请日：2014-07-28
申请人： Jones M. Hyman , John D. Walsh , Thurman C. Thorpe
发明人： Jones M. Hyman , John D. Walsh , Thurman C. Thorpe
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04
摘要： The present invention relates to methods and systems for scanning, detecting, and monitoring microorganisms on solid or semi-solid media using intrinsic fluorescence (IF) measurements. The methods are further directed to detection, characterization and/or identification of microorganisms on a solid or semi-solid media using intrinsic fluorescence (IF) measurements that are characteristic of said microorganisms.

6. 发明授权

US5795773A Device for detecting microorganisms 失效
标题翻译：微生物检测装置
公开(公告)号：US5795773A
公开(公告)日：1998-08-18
申请号：US823279
申请日：1997-03-24
申请人： Karen A. Read , David E. Trogdon , Thurman C. Thorpe , Christopher S. Ronsick , Scott R. Jeffrey
发明人： Karen A. Read , David E. Trogdon , Thurman C. Thorpe , Christopher S. Ronsick , Scott R. Jeffrey
IPC分类号： C12M1/24 , C12M1/34 , C12Q1/04 , C12Q1/22 , G01N21/25
CPC分类号： G01N21/253 , C12M23/08 , C12M23/38 , C12M29/20 , C12M41/26 , C12M41/36 , C12Q1/04
摘要： A method and a sealable, sterilizable vessel for detecting the presence of microorganisms in a specimen, the vessel containing a liquid culture medium and a sensor with an indicator medium therein. A gas permeable membrane with a gas impermeable removable seal thereon are provided in a wall of the vessel. If the microorganism to be detected is an anaerobic organism, then the gas impermeable seal is left in place. If the microorganism to be detected is an aerobic organism, then the gas impermeable seal is removed so as to allow the passage of oxygen into the vessel. In a preferred embodiment, the gas permeable membrane allows the passage of oxygen into the vessel while at least partly restricting the passage of carbon dioxide to outside of the vessel. Changes in the indicator medium resulting from a change in gas production (CO.sub.2, NH.sub.2, H.sub.2 S, etc.), volatile acid production or pH concentration in the medium, are detected from outside the vessel.
摘要翻译：一种用于检测样品中微生物存在的方法和可密封的可灭菌容器，所述容器含有液体培养基和其中具有指示剂介质的传感器。在容器的壁上设置有其上具有气体不可渗透的可移除密封的透气膜。如果要检测的微生物是厌氧生物，则将不透气密封留在原位。如果要检测的微生物是有氧生物体，则除去气体不可渗透的密封，以允许氧气进入容器。在优选的实施方案中，透气膜允许氧气进入容器，同时至少部分地限制二氧化碳通过容器的外部。从容器外部检测到由气体产生（CO2，NH2，H2S等）变化引起的指示剂介质的变化，介质中的挥发性酸产生或pH浓度的变化。

7. 发明授权

US07183073B2 Method for detecting microorganisms 有权
标题翻译：微生物检测方法
公开(公告)号：US07183073B2
公开(公告)日：2007-02-27
申请号：US10428655
申请日：2003-05-02
申请人： Jones M. Hyman , Paul M. Matsumura , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe
发明人： Jones M. Hyman , Paul M. Matsumura , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe
IPC分类号： C12Q1/02 , C12Q1/04 , C12N11/02 , C12N11/10 , C12N11/08 , C12M1/34
CPC分类号： C12M25/14 , C12Q1/04 , C12Q1/045 , C12Q1/24
摘要： A device and method are provided for isolating and culturing microorganisms from a bulk fluid sample. The device comprises a container having therein a polymeric immobilization layer having interstitial spaces between polymer chains such as a gel matrix. The interstitial spaces are of an average size less than an average size of microorganisms to be separated from the sample and cultured. A bulk fluid sample is applied to the immobilization layer where fluid is absorbed by the layer and microorganisms remain on the surface of the layer. After culturing, microorganism colonies are readily accessible on the surface of the layer for harvest and testing. The immobilization layer may contain one or more of nutrients for microorganisms growth, lytic agents, lytic enzymes, antibiotics, antibiotic neutralizers, indicators, detergents and selective agents. An adjacent support layer may be above and/or below the immobilization layer. The immobilization layer may be in combination with a sensor layer that changes color in areas corresponding to portions of the layer having microorganisms thereon. A membrane may be embedded in the immobilization layer for enhancing microorganism visibility and facilitating microorganism harvest.
摘要翻译：提供了用于从大量流体样品中分离和培养微生物的装置和方法。该装置包括其中具有聚合物固定化层的容器，其在聚合物链之间具有间隙，例如凝胶基质。间隙空间的平均尺寸小于从样品中分离的微生物的平均尺寸并进行培养。将大量流体样品施加到固定层，其中流体被层吸收，微生物保留在层的表面上。培养后，微生物菌落易于在层的表面进行收获和测试。固定层可以含有一种或多种用于微生物生长的营养物，溶解剂，溶解酶，抗生素，抗生素中和剂，指示剂，洗涤剂和选择剂。相邻的支撑层可以在固定层的上方和/或下方。固定层可以与在其上具有微生物层的部分相对应的区域中改变颜色的传感器层组合。膜可以嵌入在固定层中以增强微生物的可见性并促进微生物收获。

8. 发明授权

US06777226B2 Sensor device for detecting microorganisms, and method therefor 有权
标题翻译：用于检测微生物的传感器装置及其方法
公开(公告)号：US06777226B2
公开(公告)日：2004-08-17
申请号：US09741402
申请日：2000-12-18
申请人： Scott R. Jeffrey , Paul M. Matsumura , Martin J. Maresch , Jones M. Hyman , Thurman C. Thorpe
发明人： Scott R. Jeffrey , Paul M. Matsumura , Martin J. Maresch , Jones M. Hyman , Thurman C. Thorpe
IPC分类号： C12M134
CPC分类号： C12Q1/04 , C12M23/04 , C12M23/22 , C12M23/24 , C12M25/06 , C12M25/14 , C12Q1/045 , C12Q1/24
摘要： A device and method allow for detecting the presence of microorganisms in clinical and non-clinical specimens. The device, a sensor, provides an environment to culture microbial organism colonies from a fluid sample, and a means to facilitate microbial detection and quantification, either manually or with an instrument. The sensor has a microorganism immobilization matrix layer and a sensor layer. Detected microbial colonies are immediately available for further testing. The sensor provides an area for accepting a fluid sample, a mechanism to immobilize the fluid sample on an interior surface of the plate, nutrients to facilitate growth of microorganisms in the sample, and a sensor for allowing the detection and/or enumeration of microorganism colonies within the sample.
摘要翻译：一种装置和方法允许检测临床和非临床标本中微生物的存在。该装置，传感器，提供了从流体样品培养微生物菌落的环境，以及手动或用仪器促进微生物检测和定量的手段。传感器具有微生物固定基质层和传感器层。检测到的微生物菌落立即可用于进一步测试。传感器提供用于接受流体样品的区域，将流体样品固定在板的内表面上的机制，营养物质以促进样品中微生物的生长，以及用于允许微生物菌落的检测和/或计数的传感器在样品内。

9. 发明授权

US06251624B1 Apparatus and method for detecting, quantifying and characterizing microorganisms 有权
标题翻译：用于检测，定量和表征微生物的装置和方法
公开(公告)号：US06251624B1
公开(公告)日：2001-06-26
申请号：US09502678
申请日：2000-02-11
申请人： Paul M. Matsumura , Jones M. Hyman , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe , William G. Barron
发明人： Paul M. Matsumura , Jones M. Hyman , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe , William G. Barron
IPC分类号： C12Q104
CPC分类号： C12M41/46 , C12M41/36 , C12Q1/04 , C12Q1/18 , G01N21/253 , G01N2035/0425
摘要： A method and apparatus for performing microbial antibiotic susceptibility testing include disposable, multi-chambered susceptibility plates and an automated plate handler and image acquisition and processing instrument is described. The susceptibility plates are inoculated with a suitable microorganism such as bacteria, fungi, protozoa, algae or viruses and anti-microbial agent(s) are applied that the microorganism is exposed to at a variety of concentrations or a gradient of each anti-microbial agent. The plates are then placed in the instrument, which monitors and measures the growth or lack thereof of the microorganisms. This data is used to determine the susceptibility of the microorganism to the antibiotics. Such a system automates anti-microbial susceptibility testing using solid media and Carbo-Bauer standardized result reporting. The system provides a level of automation previously associated only with broth micro dilution testing, while retaining the advantages of the manual disk diffusion test.
摘要翻译：用于进行微生物抗生素敏感性试验的方法和装置包括一次性多室敏感性板和自动板处理器以及图像采集和处理仪器。敏感性板用合适的微生物如细菌，真菌，原生动物，藻类或病毒接种，并且应用抗微生物剂，微生物以各种浓度或每种抗微生物剂的梯度暴露。然后将板放置在仪器中，其监测和测量微生物的生长或缺乏。该数据用于确定微生物对抗生素的敏感性。这种系统使用固体培养基和Carbo-Bauer标准化结果报告自动化抗微生物敏感性测试。该系统提供以前仅与肉汤微量稀释测试相关的自动化水平，同时保留手动盘扩散测试的优点。

10. 发明授权

US06596532B1 Device for isolation and surface culture of microorganisms from bulk fluids 有权
标题翻译：用于从散装流体中分离和表面培养微生物的装置
公开(公告)号：US06596532B1
公开(公告)日：2003-07-22
申请号：US09502324
申请日：2000-02-11
申请人： Jones M. Hyman , Paul M. Matsumura , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe
发明人： Jones M. Hyman , Paul M. Matsumura , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe
IPC分类号： C12M134
CPC分类号： C12M25/14 , C12Q1/04 , C12Q1/045 , C12Q1/24
摘要： A device and method are provided for isolating and culturing microorganisms from a bulk fluid sample. The device comprises a container having therein a polymeric immobilization layer having interstitial spaces between polymer chains such as a gel matrix. The interstitial spaces are of an average size less than an average size of microorganisms to be separated from the sample and cultured. A bulk fluid sample is applied to the immobilization layer where fluid is absorbed by the layer and microorganisms remain on the surface of the layer. After culturing, microorganism colonies are readily accessible on the surface of the layer for harvest and testing. The immobilization layer may contain one or more of nutrients for microorganism growth, lytic agents, lytic enzymes, antibiotics, antibiotic neutralizers, indicators, detergents and selective agents. An adjacent support layer may be above and/or below the immobilization layer. The immobilization layer may be in combination with a sensor layer that changes color in areas corresponding to portions of the layer having microorganisms thereon. A membrane may be embedded in the immobilization layer for enhancing microorganism visibility and facilitating microorganism harvest.
摘要翻译：提供了用于从大量流体样品中分离和培养微生物的装置和方法。该装置包括其中具有聚合物固定化层的容器，其在聚合物链之间具有间隙，例如凝胶基质。间隙空间的平均尺寸小于从样品中分离的微生物的平均尺寸并进行培养。将大量流体样品施加到固定层，其中流体被层吸收，微生物保留在层的表面上。培养后，微生物菌落易于在层的表面进行收获和测试。固定层可以含有一种或多种用于微生物生长的营养物，溶解剂，溶解酶，抗生素，抗生素中和剂，指示剂，洗涤剂和选择剂。相邻的支撑层可以在固定层的上方和/或下方。固定层可以与在其上具有微生物层的部分相对应的区域中改变颜色的传感器层组合。膜可以嵌入在固定层中以增强微生物的可见性并促进微生物的收获。

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