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    • 4. 发明专利
    • PHOTOELECTRON SPECTROSCOPY FOR SOLID-STATE SURFACE
    • JPH01199140A
    • 1989-08-10
    • JP2447688
    • 1988-02-04
    • RIKAGAKU KENKYUSHO
    • MUNAKATA TOSHIAKIKASUYA KEIKO
    • G01N23/227G01N21/00
    • PURPOSE:To enable the highly sensitive measurement of an excitation level on a solid-state surface, by setting the intensity of vacuum ultraviolet laser light at a value whereat the pumping rate thereof exceeds a relaxation time of energy of an electron excited to a non-occupation level. CONSTITUTION:Laser light generated in a vacuum ultraviolet light generating chamber 12 and made to enter a vacuum ultraviolet spectroscope 13 is rid of a fundamental wave and only short wavelength light thereof is introduced into an ultra-high vacuum electron spectroscope 14. Being matched with a time of irradiation of a sample 15 by this short wavelength light, a wavelength-variable laser light for surface excitation is generated from a wavelength-variable laser oscillator 16 through a timing control 10 and applied to the sample 15 and thereby electrons in a solid-state surface are excited to a non-occupation level. The electrons emittted from the surface of the sample 15 are detected by an electron energy analyzer 17 and an electron detector 18, counted by an electron counting device 21 and sent to a computer 20, where the state of the electrons in the solid-state surface is measured. Then, the intensity of the vacuum ultraviolet laser light is set at a value whereat the pumping rate thereof exceeds a relaxation time of energy of an electron excited to the non-occupation level.
    • 7. 发明专利
    • FLUORO-MICROSCOPIC SPECTRAL METHOD AND APPARATUS
    • JPH01102342A
    • 1989-04-20
    • JP26081987
    • 1987-10-16
    • RIKAGAKU KENKYUSHO
    • TSUKAGOSHI MIKIROKASUYA KEIKO
    • G01N21/64
    • PURPOSE:To enable the measurement of the quantity of a fluorescent material in a unit volume, by scanning a sample two-dimensionally by a converged laser light. CONSTITUTION:In an optical system, a laser light oscillated from a laser light source 1 is introduced into a modulation element 3. In a detecting system, the laser light is subjected to amplitude modulation by a photoacoustic deflector in the element 3 so as to measure the intensity of fluorescence from a sample 10. A scanner mirror 6 receives a signal from CPU 18 by a driving power source 8 and projects same onto a condenser lens 9 while executing a two-dimensional scanning, so as to irradiate the surface of the sample 10 two-dimensionally by a micro-spot light. Moreover, a sample stage 7 is provided with a rough two-dimensional drive system, which enables the search of the sample 10 in the visual field of a microscope by a driving power source 11 in linkage to the CPU 18. On the other side, the detecting system is provided with photomultiplier tubes 16a-16c, whereby the intensity of a fluorescent light separated into its components by BPF 15a and 15b is converted into an electric signal, amplified 17 and introduced into the CPU 18. Besides, a control-processing system subjects a pulse generated from the CPU 18 to A/D conversion, giving a signal to the power source 8 for two-dimensional scanning.
    • 10. 发明专利
    • FREEZE-STORAGE OF INSECT OVUM
    • JPS6468301A
    • 1989-03-14
    • JP22620187
    • 1987-09-09
    • RIKAGAKU KENKYUSHO
    • KURODA YUKIAKITAKADA YUKOKASUYA KEIKO
    • A01K67/04A01K67/00A01K67/033A01N1/02
    • PURPOSE:To enable the freeze storage of insect ovum preventing the destruction of life-sustaining structure and enable the permanent stock preservation of insect ovum by irradiating laser beam to an insect ovum, introducing a freeze- protection liquid into the insect ovum and storing the insect ovum in frozen state. CONSTITUTION:Ovum of an insect such as Drosophila melanogaster, silk worm, rare insect, agricultural vermin or hygienic vermin is stored in frozen state by irradiating laser beam, preferably focused laser beam, especially pulse laser beam to the insect ovum, especially the insect ovum subjected to removal of shell membrane, preferably at a specific position in a state immersed in a freeze- protection liquid or prior to the immersion in a freeze-protection liquid. The surface of the insect ovum is modified and the freeze-protection liquid is introduced into the insect ovum by this treatment to prevent the destruction of the life-sustaining structure composed of minute organs is vitelline membrane (e.g. nucleus, mitochondria and microsome) caused by the freezing of water in the ovum to form sharp acicular crystals.