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1. 发明授权

US07407783B2 Fructosylamine oxidase 有权
标题翻译：果糖胺氧化酶
公开(公告)号：US07407783B2
公开(公告)日：2008-08-05
申请号：US10528992
申请日：2003-09-16
申请人： Nobuyuki Yoshida , Yoshiki Tani , Satoshi Yonehara
发明人： Nobuyuki Yoshida , Yoshiki Tani , Satoshi Yonehara
IPC分类号： C12N9/06 , C12N9/00 , C12N1/20 , C12N15/00 , C12Q1/00 , C12Q1/42 , C07K1/00 , C07H21/04
CPC分类号： C12N9/0022 , C12Q1/26
摘要： The present invention provides a fructosylamine oxidase which is obtainable by culturing Fusarium proliferatum, and purifying two types of fructosylamine oxidase (FAO) with different substrate specificities from the culture, and which is useful in the measurement of amadori compounds.
摘要翻译：本发明提供了可通过培养镰刀菌（Fusarium proliferatum）获得的果糖胺氧化酶，并从培养物中纯化具有不同底物特异性的两种类型的果糖胺氧化酶（FAO），其可用于测量阿马多利化合物。

2. 发明申请

US20060172367A1 Fructosylamine oxidase 有权
标题翻译：果糖胺氧化酶
公开(公告)号：US20060172367A1
公开(公告)日：2006-08-03
申请号：US10528992
申请日：2003-09-16
申请人： Nobuyuki Yoshida , Yoshiki Tani , Satoshi Yonehara
发明人： Nobuyuki Yoshida , Yoshiki Tani , Satoshi Yonehara
IPC分类号： C12Q1/30 , C07H21/04 , C12P21/06 , C12N9/06 , C12N15/74 , C12N1/21
CPC分类号： C12N9/0022 , C12Q1/26
摘要： The present invention provides a fructosylamine oxidase which is obtainable by culturing Fusarium proliferatum, and purifying two types of fructosylamine oxidase (FAO) with different substrate specificities from the culture, and which is useful in the measurement of amadori compounds.
摘要翻译：本发明提供了可通过培养镰刀菌（Fusarium proliferatum）获得的果糖胺氧化酶，并从培养物中纯化具有不同底物特异性的两种类型的果糖胺氧化酶（FAO），其可用于测量阿马多利化合物。

3. 发明授权

US09121821B2 Process for analyzing sample by capillary electrophoresis method 有权
标题翻译：毛细管电泳法分析样品的方法
公开(公告)号：US09121821B2
公开(公告)日：2015-09-01
申请号：US12376739
申请日：2007-08-29
申请人： Yoshihide Tanaka , Shinichi Wakida , Yusuke Nakayama , Satoshi Yonehara
发明人： Yoshihide Tanaka , Shinichi Wakida , Yusuke Nakayama , Satoshi Yonehara
IPC分类号： G01N27/447
CPC分类号： G01N27/447 , G01N27/44756
摘要： A process for analyzing a sample by a capillary electrophoresis method is provided that allows for high analytic precision and reduction in apparatus size, and can be readily carried out by electrophoresing a complex of a sample and an anionic group-containing compound in the capillary channel, wherein the capillary channel includes an A layer that is coated on an inner wall thereof and a B layer that is coated on the A layer, where the A and B layers are as described.
摘要翻译：提供了通过毛细管电泳方法分析样品的方法，其允许高分析精度和装置尺寸的降低，并且可以容易地通过在毛细通道中电泳样品和含阴离子基团的化合物的络合物来进行，其中毛细通道包括涂覆在其内壁上的A层和涂覆在A层上的B层，其中A层和B层如上所述。

4. 发明授权

US08361292B2 Method for analyzing hemoglobin by capillary electrophoresis and additive used therein 有权
标题翻译：通过毛细管电泳分析血红蛋白的方法及其中使用的添加剂
公开(公告)号：US08361292B2
公开(公告)日：2013-01-29
申请号：US12514293
申请日：2008-04-23
申请人： Yusuke Nakayama , Satoshi Yonehara
发明人： Yusuke Nakayama , Satoshi Yonehara
IPC分类号： G01N27/447
CPC分类号： G01N27/44747
摘要： The present invention provides a method for analyzing hemoglobin by capillary electrophoresis, that allows the apparatus to be smaller in size, allows a highly precise analysis to be obtained, and allows the analysis to be performed in a short period of time. The analytical method of the present invention are methods for analyzing hemoglobin by capillary electrophoresis, comprising: a sample-providing step of providing a sample containing hemoglobin; a capillary tube-providing step of providing a capillary tube containing a buffer solution; and an electrophoresis step of carrying out electrophoresis of the sample, by introducing the sample into the buffer solution in the capillary tube, and applying a voltage across both ends of the capillary tube; wherein the electrophoresis is carried out following at least one of modes (A) and (B) below: (A) the electrophoresis is carried out with a surfactant (a) added to the buffer solution, the surfactant (a) being a non-ionic surfactant having an alkyl group as a hydrophobic portion and a sugar as a hydrophilic portion; and (B) the electrophoresis is carried out with a surfactant (b) added to the sample, the surfactant (b) being a betaine-type amphoteric surfactant.
摘要翻译：本发明提供了一种通过毛细管电泳分析血红蛋白的方法，其允许装置尺寸更小，可以获得高精度分析，并允许在短时间内进行分析。本发明的分析方法是通过毛细管电泳分析血红蛋白的方法，包括：提供含有血红蛋白的样品的样品提供步骤; 毛细管提供步骤，提供含有缓冲溶液的毛细管; 以及电泳步骤，通过将样品引入毛细管中的缓冲溶液中并在毛细管的两端施加电压，进行样品的电泳; 其中所述电泳按照以下模式（A）和（B）中的至少一种进行：（A）用添加到缓冲溶液中的表面活性剂（a）进行电泳，所述表面活性剂（a）具有作为疏水部分的烷基和作为亲水部分的糖的离子表面活性剂; （B）用表面活性剂（b）进行电泳，表面活性剂（b）为甜菜碱型两性表面活性剂。

5. 发明授权

US08137512B2 Process for analyzing sample by capillary electrophoresis method 有权
标题翻译：毛细管电泳法分析样品的方法
公开(公告)号：US08137512B2
公开(公告)日：2012-03-20
申请号：US12376744
申请日：2007-08-29
申请人： Yoshihide Tanaka , Shinichi Wakida , Yusuke Nakayama , Satoshi Yonehara
发明人： Yoshihide Tanaka , Shinichi Wakida , Yusuke Nakayama , Satoshi Yonehara
IPC分类号： B01D57/02 , B01D59/42 , C02F1/469 , C07K1/26
CPC分类号： G01N27/447 , G01N27/44756
摘要： A process for analyzing a sample by a capillary electrophoresis method is provided that allows the apparatus to be reduced in size, allows a high analytical precision to be obtained, and can be carried out easily. The analytical process of the present invention is a process for analyzing a sample by a capillary electrophoresis method. The analytical process includes preparing a capillary tube to be used for the capillary electrophoresis method, and performing electrophoretic separation of a complex of a sample and an anionic group-containing compound that are bonded together, in the capillary tube, wherein the capillary tube includes an anionic layer that is formed of the anionic group-containing compound and that is coated on the inner wall of the capillary tube, and the anionic layer is fixed to the inner wall of the capillary tube by a covalent bond.
摘要翻译：提供了一种通过毛细管电泳方法分析样品的方法，其允许减小装置的尺寸，允许获得高分析精度，并且可以容易地进行。本发明的分析方法是通过毛细管电泳法分析样品的方法。分析方法包括制备用于毛细管电泳方法的毛细管，并且在毛细管中进行电泳分离粘合在一起的样品和含阴离子基团的化合物的络合物，其中毛细管包括阴离子层，其由含阴离子基团的化合物形成并被涂布在毛细管的内壁上，阴离子层通过共价键固定在毛细管的内壁上。

6. 发明授权

US08008085B2 Method of measuring HbA1c 有权
标题翻译：测量HbA1c的方法
公开(公告)号：US08008085B2
公开(公告)日：2011-08-30
申请号：US12377232
申请日：2008-01-30
申请人： Satoshi Yonehara , Norio Inamura
发明人： Satoshi Yonehara , Norio Inamura
IPC分类号： G01N33/72 , G01N33/48
CPC分类号： C12Q1/26 , C12Q1/37 , G01N33/721 , G01N2333/805 , Y10T436/19 , Y10T436/25
摘要： A method of measuring HbA1c is provided that, even with a whole blood sample after storage, measurement accuracy substantially equal to a whole blood sample right after collection can be maintained. Whole blood is stored in a presence of a glycolytic inhibitor and protease is added to the stored whole blood sample to cleave hemoglobin in the whole blood sample. Then a glycated part of a hemoglobin fragment thereby obtained is treated with fructosyl amine oxidase. Thereafter, a glycation degree of HbA1c is determined by measuring a redox reaction between the glycated part and the fructosyl amine oxidase. Further, instead of storage of the whole blood in a presence of the glycolytic inhibitor, a strong electrolyte substance such as KCl, K2SO4, KNO, NaCl, Na2SO4, NaNO, MgCl2, MgSO4, Mg(NO)2, etc. is added to the whole blood after storage and a protease treatment is performed in a presence of the strong electrolyte substance. According to these methods, fluctuation in a measurement value of HbA1c due to storage of the whole blood can be avoided.
摘要翻译：提供一种测定HbA1c的方法，即使在储存后的全血样品中，也可以维持与收集后的全血样本基本相等的测定精度。将全血存储在糖酵解抑制剂的存在下，并将蛋白酶加入到储存的全血样品中，以切割全血样品中的血红蛋白。然后将由此获得的血红蛋白片段的糖化部分用果糖胺氧化酶处理。此后，通过测量糖化部分和果糖胺氧化酶之间的氧化还原反应来测定HbA1c的糖化度。此外，代替在糖酵解抑制剂存在下全血的储存，加入强电解质物质如KCl，K 2 SO 4，KNO，NaCl，Na 2 SO 4，NaNO，MgCl 2，MgSO 4，Mg（NO）2等储存后的全血和蛋白酶处理在强电解质物质的存在下进行。根据这些方法，可以避免由于全血的储存引起的HbA1c测量值的波动。

7. 发明申请

US20100116660A1 Electrophoresis Chip, Electrophoresis Apparatus, and Method for Analyzing Sample by Capillary Electrophoresis 有权
标题翻译：电泳芯片，电泳装置和毛细管电泳分析样品的方法
公开(公告)号：US20100116660A1
公开(公告)日：2010-05-13
申请号：US12515994
申请日：2008-04-28
申请人： Yoshihide Tanaka , Yusuke Nakayama , Satoshi Yonehara
发明人： Yoshihide Tanaka , Yusuke Nakayama , Satoshi Yonehara
IPC分类号： G01N27/447
CPC分类号： G01N27/44791 , G01N27/44743
摘要： An electrophoresis chip that can be small and simple and that can analyze a sample with high accuracy is provided. The electrophoresis chip includes an upper substrate 4, a lower substrate 1, an introduction reservoir 2a, a recovery reservoir 2b and a capillary channel for sample analysis 3x. The introduction reservoir 2a and the recovery reservoir 2b are formed in the lower substrate 1. The introduction reservoir 2a and the recovery reservoir 2b are in communication with each other via the capillary channel for sample analysis 3x. The introduction reservoir 2a receives a sample to be measured. The sample is electrophoretically introduced directly into the capillary channel for sample analysis 3x by creating a potential difference between the introduction reservoir 2a and the recovery reservoir 2b, and is also analyzed in the capillary channel for sample analysis 3x during the separation of the sample while the sample is being continuously supplied.
摘要翻译：提供可以小而简单并且可以高精度地分析样品的电泳芯片。电泳芯片包括上基板4，下基板1，导入槽2a，回收槽2b和用于样品分析的毛细通道3x。引入储存器2a和回收储存器2b形成在下基板1中。引入储存器2a和回收储存器2b经由用于样本分析3x的毛细通道彼此连通。引入容器2a接收要测量的样品。通过在引入储存器2a和回收储存器2b之间产生电位差，将样品电泳引入毛细通道3x进行样品分析，并在样品分离过程中在毛细管通道中进行样品分析3x分析，同时样品正在连续供应。

8. 发明授权

US07201871B2 Specimen having capability of separating solid component 有权
标题翻译：样品具有分离固体成分的能力
公开(公告)号：US07201871B2
公开(公告)日：2007-04-10
申请号：US10221644
申请日：2001-03-15
申请人： Kaoru Hirai , Satoshi Yonehara
发明人： Kaoru Hirai , Satoshi Yonehara
IPC分类号： G01N30/00
CPC分类号： G01N21/78 , G01N21/8483 , G01N33/5002 , G01N33/521 , Y10T436/25375 , Y10T436/255
摘要： A test strip having the capability of separating blood cells from whole blood, wherein it has a porous reagent layer comprising beads, an inorganic gel and a reagent that causes a detectable reaction with a substance to be detected, and a substrate that supports the reagent layer, wherein the beads are adhered to each other with the inorganic gel, and interstices are formed between the beads to trap a solid. The test strip can thus be used for separating blood cells from a whole blood including blood cells and plasma and detecting a substance to be detected, such as glucose, contained in plasma. The use of the test strip allows the measurement of a substance to be detected even by measurement of a transmitted light, the exhibition of good oxygen permeability, and thus can be used for measuring a substance to be detected with improved accuracy.
摘要翻译：具有从全血中分离血细胞的能力的测试条，其中具有多孔试剂层，其包含珠子，无机凝胶和导致与被检测物质的可检测反应的试剂，以及支持试剂层的基材其中珠子用无机凝胶彼此粘合，并且在珠粒之间形成间隙以捕获固体。因此，测试条可用于从包括血细胞和血浆的全血中分离血细胞并检测血浆中所含的待检测物质如葡萄糖。测试条的使用允许即使通过测量透射光来测量待检测物质，也可以显示良好的透氧性，因此可以用于以更高的精度测量待检测物质。

9. 发明授权

US06352835B1 Method of measuring substance in sample using a redox reaction 有权
标题翻译：使用氧化还原反应测量样品中物质的方法
公开(公告)号：US06352835B1
公开(公告)日：2002-03-05
申请号：US09440241
申请日：1999-11-15
申请人： Tsuguki Komori , Satoshi Yonehara
发明人： Tsuguki Komori , Satoshi Yonehara
IPC分类号： C12Q126
CPC分类号： C12Q1/28 , G01N33/725
摘要： A highly reliable method of measuring an analyte in a sample using a redox reaction. In this method, a tetrazolium compound is added to a sample prior to the redox reaction so as to eliminate the influence of any reducing substance in the sample, then a reducing substance or an oxidizing substance derived from the analyte is formed, the quantity of the formed substance derived from the analyte is measured by the redox reaction, and the quantity of the analyte is determined from the quantity of the formed substance derived from the analyte. As the tetrazolium compound, for example, 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt can be used.
摘要翻译：使用氧化还原反应测量样品中分析物的高度可靠的方法。在该方法中，在氧化还原反应之前将四唑化合物加入到样品中，以消除样品中任何还原物质的影响，然后形成来自分析物的还原物质或氧化物质，通过氧化还原反应测定来自分析物的成膜物质，根据来自分析物的形成物质的量求出分析物的量。作为四唑鎓化合物，例如可以使用2-（4-碘苯基）-3-（2,4-二硝基苯基）-5-（2,4-二磺基苯基）-2H-四唑鎓盐。

10. 发明授权

US5985591A Method for the determination of glycosylated proteins 有权
标题翻译：测定糖基化蛋白质的方法
公开(公告)号：US5985591A
公开(公告)日：1999-11-16
申请号：US196663
申请日：1998-11-19
申请人： Satoshi Yonehara , Tsuguki Komori
发明人： Satoshi Yonehara , Tsuguki Komori
IPC分类号： C12Q1/26 , C12Q1/32 , C12Q1/37 , C12Q1/28 , C12Q1/00
CPC分类号： C12Q1/32 , C12Q1/26 , C12Q1/37 , G01N2333/795 , G01N2333/80
摘要： A method for measuring the amount of glycosylated protein comprising the steps of decomposing glycosylated proteins with protease, causing a redox reaction between the decomposed products and an Amadori compound oxidoreductase, and determining the redox reaction so as to measure the amount of glycosylated proteins, wherein the decomposition with the protease is carried out in the presence of at least one substrate selected from the group consisting of metalloporphyrin, cytochrome and diaphorase.
摘要翻译：一种测定糖基化蛋白质量的方法，包括以下步骤：用蛋白酶分解糖基化蛋白质，引起分解产物与阿马多利复合氧化还原酶之间的氧化还原反应，并测定氧化还原反应以测量糖基化蛋白质的量，其中蛋白酶的分解是在至少一种选自金属卟啉，细胞色素和心肌黄酶的底物的存在下进行的。

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