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    • 3. 发明授权
    • Device for forming tissue pieces for tissue array
    • 用于形成组织阵列的组织片的装置
    • US09335238B2
    • 2016-05-10
    • US13810660
    • 2011-07-26
    • Yoshiaki NakazawaMasaki TakanoMasahiko ArakawaKohji Naritake
    • Yoshiaki NakazawaMasaki TakanoMasahiko ArakawaKohji Naritake
    • G01N1/30G01N1/28G01N1/36C12M1/33
    • G01N1/30C12M45/02G01N1/286G01N1/36
    • The invention provides a method for forming tissue pieces which allows stable and accurate winding around a core rod in rolling a sheet-like tissue piece, and a device therefor.In the tissue pieces used as each tissue piece in a tissue array obtained by arranging the tissue pieces on a substrate in an array form, the method for forming a roll-shaped tissue piece 6 obtained by rolling a sheet-like tissue piece 8 is characterized by the facts that the sheet-like tissue piece 8 is produced by slicing a tissue block 5 obtained by embedding the tissues with an embedding medium, the sheet-like tissue piece 8 is placed on a mounting block 12, peeling means 13 which easily enables peeling of the tissue piece 8 from the mounting block 12 is applied between the mounting block 12 and the tissue piece 8, and the tissue piece 8 is wound around a core rod 11 while heating the tissue piece 8 on the mounting block 12.
    • 本发明提供了一种用于形成组织片的方法,其允许在卷绕片状组织片时围绕芯棒进行稳定和精确的缠绕,以及用于其的装置。 在通过将组织块以阵列形式布置在基板上而获得的组织排列中用作每个组织片的组织片中,形成通过滚动片状组织片8获得的辊状组织片6的方法的特征在于 通过切片通过用嵌入介质包埋组织获得的组织块5来生产片状组织片8,片状组织片8被放置在安装块12上,剥离装置13容易启用 将组织片8从安装块12剥离施加在安装块12和组织片8之间,并且组织片8缠绕在芯棒11上,同时加热安装块12上的组织片8。
    • 5. 发明授权
    • Tissue array production method
    • 组织阵列生产方法
    • US08835130B2
    • 2014-09-16
    • US13810662
    • 2011-07-26
    • Yoshiaki NakazawaMasaki TakanoMasahiko ArakawaKohji Naritake
    • Yoshiaki NakazawaMasaki TakanoMasahiko ArakawaKohji Naritake
    • C12N5/00G01N33/48G01N1/06G01N1/36
    • G01N1/36G01N1/06G01N2001/362G01N2001/368
    • Tissue array production method enables even roll-shaped tissue pieces having various diameters to be steadily fixed to a substrate block is provided. In a method in which roll-shaped tissue pieces formed by rolling sheet-like tissue pieces in the shape of a roll are arranged on a substrate in an array form, a tissue array is produced by placing a holding member which holds a plurality of roll-shaped tissue pieces so that their axis directions are vertically directed in a container into which a melted embedding medium is poured for its accumulation, to hold the respective roll-shaped tissue pieces in the holding member; pouring the embedding medium into the container, to form a substrate block constituted so that the plurality of roll-shaped tissue pieces is arranged in the array form; and slicing the substrate block so that the roll-shaped tissue pieces are ring-shaped.
    • 提供组织阵列制造方法,能够将具有各种直径的均匀的辊状组织块稳定地固定在基板块上。 在将辊状的片状组织片形成为卷状的卷状组织片以阵列形式布置在基板上的方法中,通过放置保持多个辊的保持部件来制造组织阵列 形状的组织片,使得它们的轴线方向垂直地指向容器中,熔融的包埋介质被浇注到容器中以便其积聚,以将各个辊状组织片保持在保持构件中; 将嵌入介质倒入容器内,形成以多个卷状的组织片排列成阵列状的构成的基板块; 并将基片块切片,使得该卷状组织片呈环状。
    • 6. 发明申请
    • Method and device for manufacturing tissue section
    • 用于制造组织切片的方法和装置
    • US20050126311A1
    • 2005-06-16
    • US10509743
    • 2003-03-26
    • Kazuo MiyazawaIwao KuroiwaMasahiko ArakawaAkira Yanagimachi
    • Kazuo MiyazawaIwao KuroiwaMasahiko ArakawaAkira Yanagimachi
    • G01N1/06G01N1/28G01N1/36G01N1/42G01N1/00G01N1/04
    • G01N1/06G01N1/36G01N1/42G01N2001/2873Y10T83/04Y10T83/2192
    • The method for manufacturing a tissue section, which is used for a sample for observation by a microscope, comprises the steps of slicing an organism specimen (12), whose form has been fixed by freezing or by using an embedding agent, along a slicing surface, characterized by: adjusting a distance between the slicing surface of the organism specimen (12) and one side of a tape (32) and a temperature difference between the organism specimen (12) and the tape (32) when the slicing of the organism specimen (12) is started, thereby a tip part of the tissue section (16) curling to the outside of the slicing surface of the organism specimen (12) is allowed to adhesively abut on one side of the tape (32) running apart from the slicing surface of the organism specimen (12); and running the tape (32) at a speed in synchronism with a slicing speed of the tissue section (16) after the tip part of the tissue section (16) is allowed to adhesively abut on the one side of the tape (32), thereby the entire tissue section (16) cut off from the organism specimen (12) can be adhered onto the one side of the tape (32).
    • 用于通过显微镜观察的样本的组织切片的制造方法包括以下步骤:通过冷冻或通过使用包埋剂将形态已经被固定的生物样本(12)沿着切片表面 其特征在于:当所述生物体的切片时,调节所述生物样本(12)的切片表面与所述带(32)的一侧之间的距离以及所述生物样本(12)和所述带(32)之间的温度差 开始试样(12),从而使生物体(12)的切片面的外侧卷曲的组织部(16)的前端被粘合地抵接在离开 生物样本(12)的切片表面; 并且在所述组织部分(16)的顶端部分被允许粘附在所述带子(32)的一侧上之后,以与所述组织部分(16)的切片速度同步的速度运行所述带(32) 从而可以将从生物样本(12)切断的整个组织部分(16)粘附到带(32)的一侧。
    • 7. 发明申请
    • METHOD FOR FORMING TISSUE PIECES FOR TISSUE ARRAY AND DEVICE FOR FORMING TISSUE PIECES FOR TISSUE ARRAY
    • 形成组织组织组织的方法和用于组织组织组织组织的装置
    • US20130122540A1
    • 2013-05-16
    • US13810660
    • 2011-07-26
    • Yoshiaki NakazawaMasaki TakanoMasahiko ArakawaKohji Naritake
    • Yoshiaki NakazawaMasaki TakanoMasahiko ArakawaKohji Naritake
    • G01N1/30
    • G01N1/30C12M45/02G01N1/286G01N1/36
    • The invention provides a method for forming tissue pieces which allows stable and accurate winding around a core rod in rolling a sheet-like tissue piece, and a device therefor.In the tissue pieces used as each tissue piece in a tissue array obtained by arranging the tissue pieces on a substrate in an array form, the method for forming a roll-shaped tissue piece 6 obtained by rolling a sheet-like tissue piece 8 is characterized by the facts that the sheet-like tissue piece 8 is produced by slicing a tissue block 5 obtained by embedding the tissues with an embedding medium, the sheet-like tissue piece 8 is placed on a mounting block 12, peeling means 13 which easily enables peeling of the tissue piece 8 from the mounting block 12 is applied between the mounting block 12 and the tissue piece 8, and the tissue piece 8 is wound around a core rod 11 while heating the tissue piece 8 on the mounting block 12.
    • 本发明提供了一种用于形成组织片的方法,其允许在卷绕片状组织片时围绕芯棒进行稳定和精确的缠绕,以及用于其的装置。 在通过将组织块以阵列形式布置在基板上而获得的组织排列中用作每个组织片的组织片中,形成通过滚动片状组织片8获得的辊状组织片6的方法的特征在于 通过切片通过用嵌入介质包埋组织获得的组织块5来生产片状组织片8,片状组织片8被放置在安装块12上,剥离装置13容易启用 将组织片8从安装块12剥离施加在安装块12和组织片8之间,并且组织片8缠绕在芯棒11上,同时加热安装块12上的组织片8。
    • 8. 发明申请
    • TISSUE ARRAY PRODUCTION METHOD
    • 组织阵列生产方法
    • US20130115652A1
    • 2013-05-09
    • US13810662
    • 2011-07-26
    • Yoshiaki NakazawaMasaki TakanoMasahiko ArakawaKohji Naritake
    • Yoshiaki NakazawaMasaki TakanoMasahiko ArakawaKohji Naritake
    • G01N1/36
    • G01N1/36G01N1/06G01N2001/362G01N2001/368
    • A tissue array production method which enables even roll-shaped tissue pieces having various diameters to be steadily fixed to a substrate block is provided.In the tissue array production method in which roll-shaped tissue pieces 6 formed by rolling sheet-like tissue pieces 8 in the shape of a roll are arranged on a substrate in an array form, the tissue array is produced by placing a holding member 16 which holds a plurality of roll-shaped tissue pieces 6 so that their axis directions are vertically directed in a container 10 into which a melted embedding medium is poured for its accumulation, to hold the respective roll-shaped tissue pieces 6 in the holding member 16; by pouring the embedding medium into the container 10, to form a substrate block 40 constituted so that the plurality of roll-shaped tissue pieces 6 is arranged in the array form; and by slicing the substrate block 40 so that the roll-shaped tissue pieces 6 are ring-shaped, to place the sliced pieces on the substrate.
    • 提供一种能够使具有各种直径的均匀的辊状组织块稳定地固定到基板块的组织阵列制造方法。 在通过将辊状的片状组织片8以辊状形成的辊状组织片6以阵列形式配置在基板上的组织排列制造方法中,通过将保持构件16 其保持多个辊状组织片6,使得它们的轴线方向垂直地指向容器10,熔融的包埋介质被浇注到容器10中以便积聚,以将各个辊状组织片6保持在保持部件16中 ; 通过将包埋介质倾倒到容器10中,形成以多个卷状组织片6排列成形状的基板块40。 并且通过切割基板块40使得卷状组织片6是环状的,以将切片放置在基板上。
    • 9. 发明授权
    • Method and device for manufacturing tissue section
    • 用于制造组织切片的方法和装置
    • US07503245B2
    • 2009-03-17
    • US10509743
    • 2003-03-26
    • Kazuo MiyazawaIwao KuroiwaMasahiko ArakawaAkira Yanagimachi
    • Kazuo MiyazawaIwao KuroiwaMasahiko ArakawaAkira Yanagimachi
    • B26D1/00
    • G01N1/06G01N1/36G01N1/42G01N2001/2873Y10T83/04Y10T83/2192
    • The method for manufacturing a tissue section, which is used for a sample for observation by a microscope, comprises the steps of slicing an organism specimen (12), whose form has been fixed by freezing or by using an embedding agent, along a slicing surface, characterized by: adjusting a distance between the slicing surface of the organism specimen (12) and one side of a tape (32) and a temperature difference between the organism specimen (12) and the tape (32) when the slicing of the organism specimen (12) is started, thereby a tip part of the tissue section (16) curling to the outside of the slicing surface of the organism specimen (12) is allowed to adhesively abut on one side of the tape (32) running apart from the slicing surface of the organism specimen (12); and running the tape (32) at a speed in synchronism with a slicing speed of the tissue section (16) after the tip part of the tissue section (16) is allowed to adhesively abut on the one side of the tape (32), thereby the entire tissue section (16) cut off from the organism specimen (12) can be adhered onto the one side of the tape (32).
    • 用于通过显微镜观察的样本的组织切片的制造方法包括以下步骤:通过冷冻或通过使用包埋剂将形态已经被固定的生物样本(12)沿着切片表面 其特征在于:当所述生物体的切片时,调节所述生物样本(12)的切片表面与所述带(32)的一侧之间的距离以及所述生物样本(12)和所述带(32)之间的温度差 开始试样(12),从而使生物体(12)的切片面的外侧卷曲的组织部(16)的前端被粘合地抵接在离开 生物样本(12)的切片表面; 并且在所述组织部分(16)的顶端部分被允许粘附在所述带子(32)的一侧上之后,以与所述组织部分(16)的切片速度同步的速度运行所述带(32) 从而可以将从生物样本(12)切断的整个组织部分(16)粘附到带(32)的一侧。