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1. 发明申请

US20050126311A1 Method and device for manufacturing tissue section 有权
标题翻译：用于制造组织切片的方法和装置
公开(公告)号：US20050126311A1
公开(公告)日：2005-06-16
申请号：US10509743
申请日：2003-03-26
申请人： Kazuo Miyazawa , Iwao Kuroiwa , Masahiko Arakawa , Akira Yanagimachi
发明人： Kazuo Miyazawa , Iwao Kuroiwa , Masahiko Arakawa , Akira Yanagimachi
IPC分类号： G01N1/06 , G01N1/28 , G01N1/36 , G01N1/42 , G01N1/00 , G01N1/04
CPC分类号： G01N1/06 , G01N1/36 , G01N1/42 , G01N2001/2873 , Y10T83/04 , Y10T83/2192
摘要： The method for manufacturing a tissue section, which is used for a sample for observation by a microscope, comprises the steps of slicing an organism specimen (12), whose form has been fixed by freezing or by using an embedding agent, along a slicing surface, characterized by: adjusting a distance between the slicing surface of the organism specimen (12) and one side of a tape (32) and a temperature difference between the organism specimen (12) and the tape (32) when the slicing of the organism specimen (12) is started, thereby a tip part of the tissue section (16) curling to the outside of the slicing surface of the organism specimen (12) is allowed to adhesively abut on one side of the tape (32) running apart from the slicing surface of the organism specimen (12); and running the tape (32) at a speed in synchronism with a slicing speed of the tissue section (16) after the tip part of the tissue section (16) is allowed to adhesively abut on the one side of the tape (32), thereby the entire tissue section (16) cut off from the organism specimen (12) can be adhered onto the one side of the tape (32).
摘要翻译：用于通过显微镜观察的样本的组织切片的制造方法包括以下步骤：通过冷冻或通过使用包埋剂将形态已经被固定的生物样本（12）沿着切片表面其特征在于：当所述生物体的切片时，调节所述生物样本（12）的切片表面与所述带（32）的一侧之间的距离以及所述生物样本（12）和所述带（32）之间的温度差开始试样（12），从而使生物体（12）的切片面的外侧卷曲的组织部（16）的前端被粘合地抵接在离开生物样本（12）的切片表面; 并且在所述组织部分（16）的顶端部分被允许粘附在所述带子（32）的一侧上之后，以与所述组织部分（16）的切片速度同步的速度运行所述带（32）从而可以将从生物样本（12）切断的整个组织部分（16）粘附到带（32）的一侧。

2. 发明授权

US07503245B2 Method and device for manufacturing tissue section 有权
标题翻译：用于制造组织切片的方法和装置
公开(公告)号：US07503245B2
公开(公告)日：2009-03-17
申请号：US10509743
申请日：2003-03-26
申请人： Kazuo Miyazawa , Iwao Kuroiwa , Masahiko Arakawa , Akira Yanagimachi
发明人： Kazuo Miyazawa , Iwao Kuroiwa , Masahiko Arakawa , Akira Yanagimachi
IPC分类号： B26D1/00
CPC分类号： G01N1/06 , G01N1/36 , G01N1/42 , G01N2001/2873 , Y10T83/04 , Y10T83/2192
摘要： The method for manufacturing a tissue section, which is used for a sample for observation by a microscope, comprises the steps of slicing an organism specimen (12), whose form has been fixed by freezing or by using an embedding agent, along a slicing surface, characterized by: adjusting a distance between the slicing surface of the organism specimen (12) and one side of a tape (32) and a temperature difference between the organism specimen (12) and the tape (32) when the slicing of the organism specimen (12) is started, thereby a tip part of the tissue section (16) curling to the outside of the slicing surface of the organism specimen (12) is allowed to adhesively abut on one side of the tape (32) running apart from the slicing surface of the organism specimen (12); and running the tape (32) at a speed in synchronism with a slicing speed of the tissue section (16) after the tip part of the tissue section (16) is allowed to adhesively abut on the one side of the tape (32), thereby the entire tissue section (16) cut off from the organism specimen (12) can be adhered onto the one side of the tape (32).
摘要翻译：用于通过显微镜观察的样本的组织切片的制造方法包括以下步骤：通过冷冻或通过使用包埋剂将形态已经被固定的生物样本（12）沿着切片表面其特征在于：当所述生物体的切片时，调节所述生物样本（12）的切片表面与所述带（32）的一侧之间的距离以及所述生物样本（12）和所述带（32）之间的温度差开始试样（12），从而使生物体（12）的切片面的外侧卷曲的组织部（16）的前端被粘合地抵接在离开生物样本（12）的切片表面; 并且在所述组织部分（16）的顶端部分被允许粘附在所述带子（32）的一侧上之后，以与所述组织部分（16）的切片速度同步的速度运行所述带（32）从而可以将从生物样本（12）切断的整个组织部分（16）粘附到带（32）的一侧。

3. 发明申请

US20090004640A1 Method of Paraffin-Penetration Treatment for Tissue Preparation and Apparatus for the Treatment 有权
标题翻译：用于组织制备的石蜡渗透处理方法和用于治疗的装置
公开(公告)号：US20090004640A1
公开(公告)日：2009-01-01
申请号：US12087341
申请日：2006-12-28
申请人： Akira Yanagimachi
发明人： Akira Yanagimachi
IPC分类号： A01N1/02 , C12M1/00
CPC分类号： G01N1/36
摘要： The present invention provides a paraffin-penetration treatment method for a tissue preparation, which is capable of enabling sufficient penetration of paraffin even into a tissue preparation, into which paraffin can hardly penetrate by a conventional paraffin-penetration treatment method. When the penetration of the paraffin into the tissue preparation is performed by immersing the tissue preparation in molten paraffin after a dehydrating and a degreasing treatments, the method comprises the steps of: immersing the tissue preparation in the molten paraffin so as to allow the paraffin to penetrate into the tissue preparation; cooling the tissue preparation so as to solidify the paraffin having penetrated thereinto; and immersing the tissue preparation in the molten paraffin again so as to melt the paraffin having penetrated thereinto, and the above described solidification treatment and melting treatment are conducted at least once for one tissue preparation.
摘要翻译：本发明提供一种用于组织制剂的石蜡渗透处理方法，其能够使石蜡充分渗透到通过常规的石蜡包埋处理方法难以渗透石蜡的组织制剂中。当通过在脱水和脱脂处理之后将组织制剂浸入熔融石蜡中进行组织制剂的渗透时，该方法包括以下步骤：将组织制剂浸入熔融石蜡中以使石蜡渗入组织制剂; 冷却组织制剂以固化已渗入其中的石蜡; 并再次将组织制剂浸入熔融石蜡中以熔化已经渗透到其中的石蜡，并且对于一个组织制剂进行上述固化处理和熔融处理至少一次。

4. 发明授权

US07850913B2 Method of paraffin-penetration treatment for tissue preparation and apparatus for the treatment 有权
标题翻译：用于组织制备的石蜡穿透处理方法和用于治疗的装置
公开(公告)号：US07850913B2
公开(公告)日：2010-12-14
申请号：US12087341
申请日：2006-12-28
申请人： Akira Yanagimachi
发明人： Akira Yanagimachi
IPC分类号： G01N21/00
CPC分类号： G01N1/36
摘要： The present invention provides a paraffin-penetration treatment method for a tissue preparation, which is capable of enabling sufficient penetration of paraffin even into a tissue preparation, into which paraffin can hardly penetrate by a conventional paraffin-penetration treatment method. When the penetration of the paraffin into the tissue preparation is performed by immersing the tissue preparation in molten paraffin after a dehydrating and a degreasing treatments, the method comprises the steps of: immersing the tissue preparation in the molten paraffin so as to allow the paraffin to penetrate into the tissue preparation; cooling the tissue preparation so as to solidify the paraffin having penetrated thereinto; and immersing the tissue preparation in the molten paraffin again so as to melt the paraffin having penetrated thereinto, and the above described solidification treatment and melting treatment are conducted at least once for one tissue preparation.
摘要翻译：本发明提供一种用于组织制剂的石蜡渗透处理方法，其能够使石蜡充分渗透到通过常规的石蜡包埋处理方法难以渗透石蜡的组织制剂中。当通过在脱水和脱脂处理之后将组织制剂浸入熔融石蜡中进行组织制剂的渗透时，该方法包括以下步骤：将组织制剂浸入熔融石蜡中以使石蜡渗入组织制剂; 冷却组织制剂以固化已渗入其中的石蜡; 并再次将组织制剂浸入熔融石蜡中以熔化已经渗透到其中的石蜡，并且对于一个组织制剂进行上述固化处理和熔融处理至少一次。

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