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1. 发明申请

WO2016070136A1 DELIVERY OF BIOMOLECULES TO IMMUNE CELLS 审中-公开
标题翻译：生物分子递送到免疫细胞
公开(公告)号：WO2016070136A1
公开(公告)日：2016-05-06
申请号：PCT/US2015/058489
申请日：2015-10-30
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
发明人： SHAREI, Armon, R. , MAO, Shirley , HARTOULAROS, George , LIU, Sophia , HEIMANN, Megan , BASTO, Pamela , SZETO, Gregory , JHUNJHUNWALA, Siddharth , IRVINE, Darrell , LANGER, Robert, S. , JENSEN, Klavs, F. , VON ANDRIAN, Ulrich, H.
IPC分类号： C12N5/078 , C12N5/0783 , C12N15/89 , C12M3/02 , B07B1/00 , B01D37/00
CPC分类号： C12M35/04 , A61K39/00 , A61K45/06 , A61K49/0017 , A61K2039/5158 , C12M23/16 , C12M35/00 , C12N5/0635 , C12N5/0636 , C12N5/0639 , C12N15/87 , C12N2501/05 , C12N2501/998 , C12N2510/00 , C12N2527/00 , G01N33/5047
摘要： A method and device for preferentially delivering a compound such as an antigen to the cytosol of an immune cell. The method comprises passing a cell suspension comprising the target immune cell through a microfluidic device and contacting the suspension with the compound(s) or payload to be delivered.
摘要翻译：用于优先将化合物如抗原递送至免疫细胞的胞质溶胶的方法和装置。该方法包括使包含目标免疫细胞的细胞悬浮液通过微流体装置并将悬浮液与要递送的化合物或有效载体接触。

2. 发明申请

WO2016115179A1 GENE EDITING THROUGH MICROFLUIDIC DELIVERY 审中-公开
标题翻译：通过微流感传递进行基因编辑
公开(公告)号：WO2016115179A1
公开(公告)日：2016-07-21
申请号：PCT/US2016/013113
申请日：2016-01-12
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
发明人： SHAREI, Armon, R. , LAJOIE, Marc , JENSEN, Klavs, F. , LANGER, Robert, S.
IPC分类号： C12Q1/00
CPC分类号： G01N33/5002 , C12M35/00 , C12N15/87 , C12Q1/00 , G01N33/48721
摘要： Gene editing can be performed by introducing gene-editing components into a cell by mechanical cell disruption. Related apparatus, systems, techniques, and articles are also described. The methods and systems of the invention solve the problem of intracellular delivery of gene editing components and gene editing complexes to target cells. The results described herein indicate that delivery of gene editing components, e.g., protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA), by mechanical disruption of cell membranes leads to successful gene editing. Because intracellular delivery of gene editing materials is a current challenge, the methods provide a robust mechanism to engineer target cells without the use of potentially harmful viral vectors or electric fields.
摘要翻译：可以通过机械细胞破坏将基因编辑成分引入细胞来进行基因编辑。还描述了相关装置，系统，技术和制品。本发明的方法和系统解决了将基因编辑组分和基因编辑复合体细胞内递送到靶细胞的问题。本文描述的结果表明通过细胞膜的机械破坏递送基因编辑组分，例如蛋白质，核糖核酸（RNA）和脱氧核糖核酸（DNA）导致成功的基因编辑。因为基因编辑材料的细胞内递送是当前的挑战，所以这些方法提供了一种可靠的机制来设计靶细胞而不使用潜在有害的病毒载体或电场。

3. 发明公开

EP3245294A1 GENE EDITING THROUGH MICROFLUIDIC DELIVERY 审中-公开
标题翻译：基因编辑通过微流体传递
公开(公告)号：EP3245294A1
公开(公告)日：2017-11-22
申请号：EP16737769.6
申请日：2016-01-12
申请人： Massachusetts Institute of Technology , President and Fellows of Harvard College
发明人： SHAREI, Armon, R. , LAJOIE, Marc , JENSEN, Klavs, F. , LANGER, Robert, S.
IPC分类号： C12Q1/00
CPC分类号： G01N33/5002 , C12M35/00 , C12N15/87 , C12Q1/00 , G01N33/48721
摘要： Gene editing can be performed by introducing gene-editing components into a cell by mechanical cell disruption. Related apparatus, systems, techniques, and articles are also described. The methods and systems of the invention solve the problem of intracellular delivery of gene editing components and gene editing complexes to target cells. The results described herein indicate that delivery of gene editing components, e.g., protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA), by mechanical disruption of cell membranes leads to successful gene editing. Because intracellular delivery of gene editing materials is a current challenge, the methods provide a robust mechanism to engineer target cells without the use of potentially harmful viral vectors or electric fields.

4. 发明公开

EP3212772A1 DELIVERY OF BIOMOLECULES TO IMMUNE CELLS 审中-公开
标题翻译：生物分子免疫细胞的传递
公开(公告)号：EP3212772A1
公开(公告)日：2017-09-06
申请号：EP15855640.7
申请日：2015-10-30
申请人： Massachusetts Institute of Technology , President and Fellows of Harvard College
发明人： SHAREI, Armon, R. , MAO, Shirley , HARTOULAROS, George , LIU, Sophia , HEIMANN, Megan , BASTO, Pamela , SZETO, Gregory , JHUNJHUNWALA, Siddharth , IRVINE, Darrell , LANGER, Robert, S. , JENSEN, Klavs, F. , VON ANDRIAN, Ulrich, H.
IPC分类号： C12N5/078 , C12N5/0783 , C12N15/89 , C12M3/02 , B07B1/00 , B01D37/00
CPC分类号： C12M35/04 , A61K39/00 , A61K45/06 , A61K49/0017 , A61K2039/5158 , C12M23/16 , C12M35/00 , C12N5/0635 , C12N5/0636 , C12N5/0639 , C12N15/87 , C12N2501/05 , C12N2501/998 , C12N2510/00 , C12N2527/00 , G01N33/5047
摘要： A method and device for preferentially delivering a compound such as an antigen to the cytosol of an immune cell. The method comprises passing a cell suspension comprising the target immune cell through a microfluidic device and contacting the suspension with the compound(s) or payload to be delivered.
摘要翻译：用于优先将化合物如抗原递送至免疫细胞的胞质溶胶的方法和装置。该方法包括使包含靶免疫细胞的细胞悬浮液通过微流体装置并使悬浮液与待递送的化合物或有效负荷接触。

5. 发明公开

EP1080394A1 ELASTOMERIC MASK AND USE IN FABRICATION OF DEVICES, INLCUDING PIXELATED ELECTROLUMINESCENT DISPLAYS 审中-公开
标题翻译：弹性体材料的MASK，利用生产设备的掩码，包括电致发光光栅化SCREENS
公开(公告)号：EP1080394A1
公开(公告)日：2001-03-07
申请号：EP99918698.4
申请日：1999-04-20
申请人： President And Fellows of Harvard College , MASSACHUSETTS INSTITUTE OF TECHNOLOGY
发明人： JACKMAN, Rebecca, J. , DUFFY, David, C. , WHITESIDES, George, M. , VAETH, Kathleen, M. , JENSEN, Klavs, F.
IPC分类号： G03F7/00 , G03F1/00 , G03F7/12 , B41C1/14
CPC分类号： C23C14/042 , B82Y10/00 , B82Y30/00 , B82Y40/00 , G03F1/00 , G03F7/0002 , G03F7/0007 , G03F7/12 , H01L27/32 , H01L27/3211 , H01L51/0011 , H01L51/56
摘要： An elastomeric mask is provided that allows deposition of a variety of materials through mask openings. The mask seals effectively against substrate surfaces, allowing simple deposition from fluid phase, gas phase, and the like or removal of material using gaseous or liquid etchants. The mask then can be simply peeled from the surface of the substrate leaving the patterned material behind. Multi-layered mask techniques are described in which openings in an upper mask allow selected openings of a lower mask to remain unshielded, while other openings of the lower mask are shielded. A first deposition step, followed by re-orientation of the upper mask to expose a different set of lower mask openings, allows selective deposition of different materials in different openings of the lower mask. Pixelated organic electroluminescent devices are provided via the described technique.

6. 发明申请

WO2017008063A1 DELIVERY OF MATERIALS TO ANUCLEATE CELLS 审中-公开
标题翻译：将材料交付给细胞
公开(公告)号：WO2017008063A1
公开(公告)日：2017-01-12
申请号：PCT/US2016/041653
申请日：2016-07-08
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY
发明人： SHAREI, Armon, R. , JENSEN, Klavs, F. , ABSHIRE, James, Robbins , NILES, Jacquin, Clarence
IPC分类号： C12M3/02 , C12N15/87 , C12N5/078
CPC分类号： C12M23/16 , A61K35/18 , C12M35/04 , C12N5/0641 , C12N5/0644 , C12N15/87 , C12N2521/00 , C12N2527/00
摘要： The current subject matter includes methods, systems, articles, and techniques to deliver material to anucleate cells, such as red blood cells. Using a rapid deformation based microfluidic system, loading of red blood cells with macromolecules of different sizes has been shown. Although delivery to some mammalian cells, such as cancer cell lines and fibroblasts had been previously demonstrated using this technique, those designs were incompatible with RBCs that have dramatically different physical properties. Through the use of smaller constriction sizes, high speeds and different buffers successful delivery to red blood cells can be achieved. By enabling robust delivery to red blood cells in a simple, scalable manner, the current subject matter can be implemented in a diversity of applications that deliver material to study red blood cell diseases and/or use red blood cells as a therapeutic platform. Related apparatus, systems, techniques, and articles are also described.
摘要翻译：目前的主题包括将材料递送到诸如红细胞的无核细胞的方法，系统，制品和技术。使用基于快速变形的微流体系统，已经显示红细胞与不同大小分子的负载。虽然以前已经使用这种技术证明了对某些哺乳动物细胞的递送，例如癌细胞系和成纤维细胞，但是这些设计与具有显着不同物理性质的RBC不相容。通过使用较小的收缩尺寸，可以实现高速度和不同缓冲液成功输送到红细胞。通过以简单，可扩展的方式实现对红细胞的稳定递送，目前的主题可以在递送材料研究红细胞疾病和/或使用红细胞作为治疗平台的多种应用中实现。还描述了相关装置，系统，技术和制品。

7. 发明申请

WO2011056929A1 CONTINUOUS FLOW SYNTHESIS OF AMINO ALCOHOLS USING MICROREACTORS 审中-公开
标题翻译：使用微生物的氨基醇的连续流动合成
公开(公告)号：WO2011056929A1
公开(公告)日：2011-05-12
申请号：PCT/US2010/055386
申请日：2010-11-04
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY , BEDORE, Matthew, W. , ZABORENKO, Nikolay , JENSEN, Klavs, F. , JAMISON, Timothy, F.
发明人： BEDORE, Matthew, W. , ZABORENKO, Nikolay , JENSEN, Klavs, F. , JAMISON, Timothy, F.
IPC分类号： C07C213/04 , C07D209/00 , C07D211/00 , B01J19/00
CPC分类号： C07C213/04 , B01J19/0093 , B01J2219/00788 , B01J2219/0081 , B01J2219/00828 , B01J2219/00873 , B01J2219/00889 , B01J2219/00891 , B01J2219/00952 , C07C2601/14 , C07C2602/08 , C07D209/08 , C07D215/26 , C07C215/30 , C07C215/44 , C07C217/30 , C07C217/32
摘要： The present invention provides various methods for the synthesis of chemical species in a microreactor environment. In some cases, reaction products of the present invention may be valuable as intermediates and/or products in pharmaceutical and polymer research. For example, the method may involve the synthesis of amino alcohols within a microchannel. Embodiment described herein may allow for reactions with significantly shorter reaction times and increased efficiency.
摘要翻译：本发明提供了在微反应器环境中合成化学物质的各种方法。在某些情况下，本发明的反应产物可能作为药物和聚合物研究中的中间体和/或产品是有价值的。例如，该方法可以涉及在微通道内合成氨基醇。本文描述的实施方案可以允许具有显着更短的反应时间和提高效率的反应。

8. 发明申请

WO2006137878A3 FLOW METHOD AND REACTOR FOR MANUFACTURING NANOCRYSTALS 审中-公开
公开(公告)号：WO2006137878A3
公开(公告)日：2006-12-28
申请号：PCT/US2005/033827
申请日：2005-09-23
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY , YEN, Brian, K., H. , GUENTHER, Axel , JENSEN, Klavs, F. , SCHMIDT, Martin , BAWENDI, Moungi, G.
发明人： YEN, Brian, K., H. , GUENTHER, Axel , JENSEN, Klavs, F. , SCHMIDT, Martin , BAWENDI, Moungi, G.
IPC分类号： H01L21/20 , H01L21/36
摘要： A population of nanocrystals having a narrow and controllable size distribution and can be prepared by a segmented-flow method.

9. 发明申请

WO2018200236A8 RECONFIGURABLE CHEMICAL SYNTHESIS SYSTEMS AND METHODS 审中-公开
公开(公告)号：WO2018200236A8
公开(公告)日：2018-11-01
申请号：PCT/US2018/027750
申请日：2018-04-16
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY
发明人： JENSEN, Klavs, F. , THOMAS III, Dale, Arlington
IPC分类号： B01J19/00 , B01J8/00 , B01L99/00 , G01N1/28 , G01N1/31 , G01N35/00
摘要： Aspects of the present disclosure relate to reconfigurable chemical synthesis systems and related components and methods. In some embodiments, the described systems comprise one or more fluidic connector units, wherein each fluidic connector unit comprises a plurality of flexible conduits. In certain cases, a system comprising one or more fluidic connector units is configured to synthesize a first chemical compound by providing a plurality of fluidic connections between a plurality of fluid outlets (e.g., outlets of chemical reagent sources, outlets of pumps) and a plurality of fluid inlets (e.g., inlets of reaction modules, inlets of pumps) through the plurality of flexible conduits. In certain cases, the system is subsequently reconfigured by resetting the system (e.g., disconnecting each fluidic connection) and/or configuring the system to synthesize a second, different chemical compound (e.g., disconnecting one or more fluidic connections and providing one or more additional fluidic connections). According to some embodiments, in order to avoid tangling the flexible conduits during reconfiguration of the system, the fluidic connections are disconnected according to certain inventive methods described herein. In certain embodiments, fluidic connections are disconnected in reverse order relative to the order in which they were formed (e.g., the newest fluidic connection is disconnected first, the oldest fluidic connection is disconnected last). In certain embodiments, certain fluidic connections are targeted for disconnection, and additional fluidic connections are disconnected if they overlap the targeted fluidic connections and were formed more recently than the targeted fluidic connections. The fluidic connection and/or disconnection steps may, in some embodiments, be performed by a robotic manipulator.

10. 发明申请

WO2017192595A1 RECONFIGURABLE MULTI-STEP CHEMICAL SYNTHESIS SYSTEM AND RELATED COMPONENTS AND METHODS 审中-公开
标题翻译：可重构的多步化学合成系统及相关组件和方法
公开(公告)号：WO2017192595A1
公开(公告)日：2017-11-09
申请号：PCT/US2017/030649
申请日：2017-05-02
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY
发明人： ADAMO, Andrea , THOMAS III, Dale, Arlington , JENSEN, Klavs, F. , JAMISON, Timothy, F. , BEDERMANN, Aaron, A. , AROH, Kosisochukwu, C.
IPC分类号： B01L3/00 , B01L99/00 , B01J8/00 , G01N27/26 , G01N27/416
摘要： The instant disclosure is related to fluidic distributors, fluidic systems, and associated methods and articles. Certain embodiments are related to fluidic distributors that comprise bays including fluidic connections with relative positions that substantially correspond to each other. In some embodiments, a fluidic distributor may comprise bays with electrical interfaces with relative positions that substantially correspond to each other.
摘要翻译：本公开涉及流体分配器，流体系统以及相关的方法和物品。某些实施例涉及流体分配器，流体分配器包括包括具有基本上彼此对应的相对位置的流体连接的隔间。在一些实施例中，流体分配器可以包括具有基本上彼此对应的相对位置的具有电接口的隔间。

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