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    • 2. 发明授权
    • Rapid analysis of plural components
    • 快速分析多个组成部分
    • US5175088A
    • 1992-12-29
    • US616766
    • 1990-11-20
    • Minoru OhashiNobuhiko ArakawaOsamu OkaKenichi NumazawaYoshio Utugi
    • Minoru OhashiNobuhiko ArakawaOsamu OkaKenichi NumazawaYoshio Utugi
    • G01N33/14G01N33/487
    • G01N33/14
    • A new method of rapidly analyzing plural substances in the presence of biological catalyzers is disclosed. The method is practiced by way of the steps of injecting both pH buffer solution and specimen into a reaction cell, successively adding a plurality of enzymes to induce up-take reaction of dissolved oxygen, causing the plurality of substances to be subjected to selective oxidation in the stepwise manner, obtaining a stepdown curve of dissolved oxygen by automatically recording the oxidative process by means of a dissolved oxygen sensor, qualitatively determining each of the substances with reference to the kind of added enzymes and the order of their addition and quantitatively determining the same with reference to the extent of decrease in dissolved oxygen. Typically, oxidation of the substances is carried out by way of two or three or further more steps. The reaction cell for performing the method is equipped with a dissolved oxygen sensor on the one side wall and its upper portion is air-tightly closed with a plug through which a fine bore is formed for the purpose of supply of buffer solution, specimen and enzymes.
    • 公开了一种在生物催化剂存在下快速分析多种物质的新方法。 该方法通过将pH缓冲溶液和样品两者注入反应池中,依次加入多种酶以引起溶解氧的上升反应,导致多种物质经受选择性氧化 通过逐步的方式,通过利用溶解氧传感器自动记录氧化过程获得溶解氧的降压曲线,根据添加的酶的种类和它们的加入顺序定性测定每种物质,并定量测定 参考溶解氧的减少程度。 通常,物质的氧化通过两个或三个或更多步骤进行。 用于执行该方法的反应池在一个侧壁上装有溶解氧传感器,其上部用塞子气密地封闭,通过该塞子形成微孔,用于提供缓冲溶液,样品和酶 。
    • 3. 发明授权
    • Method for analyzing plural oxidizable components in a liquid
    • 分析液体中多种可氧化成分的方法
    • US4725539A
    • 1988-02-16
    • US584347
    • 1984-02-28
    • Minoru OhashiNobuhiko ArakawaOsamu OkaKenichi NumazawaYoshio Utugi
    • Minoru OhashiNobuhiko ArakawaOsamu OkaKenichi NumazawaYoshio Utugi
    • G01N27/416C12Q1/00C12Q1/26G01N33/487C12Q1/62C12Q1/42C12Q1/48
    • C12Q1/26C12Q1/001
    • A new method of rapidly analyzing plural substances in the presence of biological catalyzers is disclosed. The method is practiced by way of the steps of injecting both pH buffer solution and specimen into a reaction cell, successively adding a plurality of enzymes to induce uptake reaction of dissolved oxygen, causing the plurality of substances to be subjected to selective oxidation in the stepwise manner, obtaining a stepdown curve of dissolved oxygen by automatically recording the oxidative process by means of a dissolved oxygen sensor, qualitatively determining each of the substances with reference to the kind of added enzymes and the order of their addition and quantitatively determining the same with reference to the extent of decrease in dissolved oxygen. Typically, oxidation of the substances is carried out by way of two or three or further more steps. The reaction cell for performing the method is equipped with a dissolved oxygen sensor on the one side wall and its upper portion is airtightly closed with a plug through which a fine bore is formed for the purpose of supply of buffer solution, specimen and enzymes.
    • 公开了一种在生物催化剂存在下快速分析多种物质的新方法。 该方法通过将pH缓冲溶液和样品两者注入反应池中,依次加入多种酶以诱导溶解氧的摄取反应来实现,使多个物质逐步进行选择性氧化 通过溶解氧传感器自动记录氧化过程获得溶解氧的降压曲线,根据添加的酶的种类和其添加的顺序对每种物质进行定性测定,并用参考文献定量测定 达到溶解氧减少的程度。 通常,物质的氧化通过两个或三个或更多步骤进行。 用于执行该方法的反应池在一个侧壁上装有溶解氧传感器,并且其上部用塞子气密地封闭,通过该塞子形成细孔以提供缓冲溶液,样品和酶。
    • 4. 发明授权
    • Ammonia elimination liquid reagent
    • 氨清除液试剂
    • US06352847B1
    • 2002-03-05
    • US09327487
    • 1999-06-08
    • Hirokazu MatsukawaOsamu OkaTuyosi FujitaKentaro Miyazaki
    • Hirokazu MatsukawaOsamu OkaTuyosi FujitaKentaro Miyazaki
    • C12N904
    • C12N9/0006C12Q1/32
    • The invention provides an ammonia elimination reagent in solution, comprising a thermo-resistant isocitrate dehydrogenase with prominent stability under conditions at high alkaline pHs. For example, the isocitrate dehydrogenase is preferably derived from the genus Thermus. For an assay system of biological substances generating a reaction product ammonia, an ammonia elimination reagent can be prepared by selecting and using the enzyme; the resulting ammonia elimination reagent can be stored in solution; additionally, the assay system can be designed in combination with both the coenzymes NAD+ and NADP+. The ammonia elimination reagent is novel and can eliminate ammonia in an extremely short time.
    • 本发明提供一种溶液中的氨清除试剂,其包含在高碱性pH下条件下具有突出稳定性的耐热异柠檬酸脱氢酶。 例如,异柠檬酸脱氢酶优选来自嗜热栖热菌属。 对于产生反应产物氨的生物物质的测定系统,可以通过选择和使用酶来制备氨消除剂; 所得氨消除剂可以储存在溶液中; 此外,测定系统可以与辅酶NAD +和NADP +两者结合设计。 氨消除剂是新颖的,可以在极短时间内消除氨。