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1. 发明授权

US5175088A Rapid analysis of plural components 失效
标题翻译：快速分析多个组成部分
公开(公告)号：US5175088A
公开(公告)日：1992-12-29
申请号：US616766
申请日：1990-11-20
申请人： Minoru Ohashi , Nobuhiko Arakawa , Osamu Oka , Kenichi Numazawa , Yoshio Utugi
发明人： Minoru Ohashi , Nobuhiko Arakawa , Osamu Oka , Kenichi Numazawa , Yoshio Utugi
IPC分类号： G01N33/14 , G01N33/487
CPC分类号： G01N33/14
摘要： A new method of rapidly analyzing plural substances in the presence of biological catalyzers is disclosed. The method is practiced by way of the steps of injecting both pH buffer solution and specimen into a reaction cell, successively adding a plurality of enzymes to induce up-take reaction of dissolved oxygen, causing the plurality of substances to be subjected to selective oxidation in the stepwise manner, obtaining a stepdown curve of dissolved oxygen by automatically recording the oxidative process by means of a dissolved oxygen sensor, qualitatively determining each of the substances with reference to the kind of added enzymes and the order of their addition and quantitatively determining the same with reference to the extent of decrease in dissolved oxygen. Typically, oxidation of the substances is carried out by way of two or three or further more steps. The reaction cell for performing the method is equipped with a dissolved oxygen sensor on the one side wall and its upper portion is air-tightly closed with a plug through which a fine bore is formed for the purpose of supply of buffer solution, specimen and enzymes.
摘要翻译：公开了一种在生物催化剂存在下快速分析多种物质的新方法。该方法通过将pH缓冲溶液和样品两者注入反应池中，依次加入多种酶以引起溶解氧的上升反应，导致多种物质经受选择性氧化通过逐步的方式，通过利用溶解氧传感器自动记录氧化过程获得溶解氧的降压曲线，根据添加的酶的种类和它们的加入顺序定性测定每种物质，并定量测定参考溶解氧的减少程度。通常，物质的氧化通过两个或三个或更多步骤进行。用于执行该方法的反应池在一个侧壁上装有溶解氧传感器，其上部用塞子气密地封闭，通过该塞子形成微孔，用于提供缓冲溶液，样品和酶。

2. 发明授权

US4725539A Method for analyzing plural oxidizable components in a liquid 失效
标题翻译：分析液体中多种可氧化成分的方法
公开(公告)号：US4725539A
公开(公告)日：1988-02-16
申请号：US584347
申请日：1984-02-28
申请人： Minoru Ohashi , Nobuhiko Arakawa , Osamu Oka , Kenichi Numazawa , Yoshio Utugi
发明人： Minoru Ohashi , Nobuhiko Arakawa , Osamu Oka , Kenichi Numazawa , Yoshio Utugi
IPC分类号： G01N27/416 , C12Q1/00 , C12Q1/26 , G01N33/487 , C12Q1/62 , C12Q1/42 , C12Q1/48
CPC分类号： C12Q1/26 , C12Q1/001
摘要： A new method of rapidly analyzing plural substances in the presence of biological catalyzers is disclosed. The method is practiced by way of the steps of injecting both pH buffer solution and specimen into a reaction cell, successively adding a plurality of enzymes to induce uptake reaction of dissolved oxygen, causing the plurality of substances to be subjected to selective oxidation in the stepwise manner, obtaining a stepdown curve of dissolved oxygen by automatically recording the oxidative process by means of a dissolved oxygen sensor, qualitatively determining each of the substances with reference to the kind of added enzymes and the order of their addition and quantitatively determining the same with reference to the extent of decrease in dissolved oxygen. Typically, oxidation of the substances is carried out by way of two or three or further more steps. The reaction cell for performing the method is equipped with a dissolved oxygen sensor on the one side wall and its upper portion is airtightly closed with a plug through which a fine bore is formed for the purpose of supply of buffer solution, specimen and enzymes.
摘要翻译：公开了一种在生物催化剂存在下快速分析多种物质的新方法。该方法通过将pH缓冲溶液和样品两者注入反应池中，依次加入多种酶以诱导溶解氧的摄取反应来实现，使多个物质逐步进行选择性氧化通过溶解氧传感器自动记录氧化过程获得溶解氧的降压曲线，根据添加的酶的种类和其添加的顺序对每种物质进行定性测定，并用参考文献定量测定达到溶解氧减少的程度。通常，物质的氧化通过两个或三个或更多步骤进行。用于执行该方法的反应池在一个侧壁上装有溶解氧传感器，并且其上部用塞子气密地封闭，通过该塞子形成细孔以提供缓冲溶液，样品和酶。

3. 发明授权

US4650752A Method for determining the freshness of fish and mollusks 失效
标题翻译：确定鱼和软体动物新鲜度的方法
公开(公告)号：US4650752A
公开(公告)日：1987-03-17
申请号：US643053
申请日：1984-08-22
申请人： Minoru Ohashi , Nobuhiko Arakawa , Tomoko Asahara , Shuichi Sakamoto
发明人： Minoru Ohashi , Nobuhiko Arakawa , Tomoko Asahara , Shuichi Sakamoto
IPC分类号： G01N33/50 , C12Q1/26 , C12Q1/34 , C12Q1/42 , C12Q1/48 , C12Q1/68 , G01N33/12
CPC分类号： G01N33/12
摘要： A rapid, accurate and simple method is disclosed for determining an index of freshness of fish and mollusks. The index of freshness is expressed as the ratio of the combined molar concentration of inosine and hypoxanthine to the total molar concentration of the decomposition products of adenosine triphosphate. Each concentration is determined electrochemically from the amount of hydrogen peroxide produced when a sample extract from fish or mollusks is subjected to the action of certain enzymes. The emzymes used for inosine and hypoxanthine concentration are nucleoside phosphorylase and xanthine oxidase and for the concentration of the decomposition products of adenosine triphosphate are alkaline phosphatase, adenylic acid kinase, AMP deaminase and adenosine deaminase in a crude extract obtained from calf intestine, nucleoside phosphorylase and xanthine oxidase. Sodium azide may be added to the sample extract to inhibit errors in measurement caused by the presence of catalase.
摘要翻译：公开了一种用于确定鱼和软体动物的新鲜度指数的快速，准确和简单的方法。新鲜度指数表示为肌苷和次黄嘌呤的组合摩尔浓度与三磷酸腺苷分解产物的总摩尔浓度的比值。当来自鱼或软体动物的样品提取物经受某些酶的作用时，从产生的过氧化氢的量电化学测定每个浓度。用于肌苷和次黄嘌呤浓度的酶是核苷磷酸化酶和黄嘌呤氧化酶，并且对于三磷酸腺苷的分解产物的浓度是从小牛肠，核苷磷酸化酶获得的粗提取物中的碱性磷酸酶，腺苷酸激酶，AMP脱氨酶和腺苷脱氨酶黄嘌呤氧化酶。可以向样品提取物中加入叠氮化钠以抑制由过氧化氢酶的存在引起的测量误差。

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