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1. 发明申请

WO2011021846A2 MULTI-SPOT METAL-DEPOSITED NUCLEIC ACID CHIP WITH NANOSTRUCTURE ARRAYS FOR DIAGNOSING CORNEAL DYSTROPHY, AND METHOD FOR PRODUCING SAME 审中-公开
标题翻译：纳米结构阵列用于诊断角膜营养不良的多点金属沉积的核酸芯片及其制备方法
公开(公告)号：WO2011021846A2
公开(公告)日：2011-02-24
申请号：PCT/KR2010005452
申请日：2010-08-18
申请人： KOREA ADVANCED INST SCI & TECH , AVELLINO CO LTD , LEE SANG YUP , YOO SO YOUNG , KIM DO KYUN , PARK TAE JUNG , YUN JUNG KUK , LEE GENE
发明人： LEE SANG YUP , YOO SO YOUNG , KIM DO KYUN , PARK TAE JUNG , YUN JUNG KUK , LEE GENE
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , B01J2219/00509 , B01J2219/00608 , B01J2219/00612 , B01J2219/00614 , B01J2219/00621 , B01J2219/00637 , B01J2219/00648 , B01J2219/00722 , B82Y30/00 , C12Q1/6883 , C12Q2600/158
摘要： The present invention relates to a multi-spot metal-deposited nucleic acid chip with nanostructure arrays for diagnosing corneal dystrophy, and more particularly, to a multi-spot metal-deposited nucleic acid chip with nanostructure arrays which uses the optical characteristics of localized surface plasmon resonance (LSPR), to a method for producing the chip, and to a multi-spot metal-deposited nucleic acid chip with nanostructure arrays for diagnosing BIGH3 gene mutations and various types of corneal dystrophies. According to the present invention, the metal-deposited nucleic acid chip with nanostructure arrays, and an analysis device including a light source, a detector, a spectrophotometer and a computer are combined to be used as a label-free biosensor based on the optical characteristics of LSPR. The multi-spot metal-deposited nucleic acid chip with nanostructure arrays for diagnosing BIGH3 gene mutations and corneal dystrophy according to the present invention diagnoses various types of corneal dystrophies, which are hereditary eye diseases, at the same time.
摘要翻译：具有纳米结构阵列的多点金属沉积核酸芯片技术领域本发明涉及用于诊断角膜营养不良的具有纳米结构阵列的多点金属沉积核酸芯片，并且更具体地涉及具有纳米结构阵列的多点金属沉积核酸芯片，其使用局部表面等离子体共振（LSPR），用于生产芯片的方法，以及具有纳米结构阵列的多点金属沉积核酸芯片，用于诊断BIGH3基因突变和各种类型的角膜营养不良。根据本发明，将具有纳米结构阵列的金属沉积核酸芯片和包括光源，检测器，分光光度计和计算机的分析装置组合以用作基于光学特性的无标记生物传感器的LSPR。根据本发明的用于诊断BIGH3基因突变和角膜营养不良的具有纳米结构阵列的多点金属沉积核酸芯片同时诊断作为遗传性眼病的各种类型的角膜营养不良。

2. 发明申请

WO2009157739A3 BIOSENSOR USING CONDUCTIVE GRAPHENES AND PREPARATION METHOD THEREOF 审中-公开
标题翻译：使用导电石墨的生物传感器及其制备方法
公开(公告)号：WO2009157739A3
公开(公告)日：2010-04-01
申请号：PCT/KR2009003482
申请日：2009-06-26
申请人： KOREA ADVANCED INST SCI & TECH , LEE SANG YUP , PARK TAE JUNG , HONG WON HI , JUNG HEE TAE , PARK HO SEOK , CHOI BONG GILL
发明人： LEE SANG YUP , PARK TAE JUNG , HONG WON HI , JUNG HEE TAE , PARK HO SEOK , CHOI BONG GILL
IPC分类号： C01B31/02
CPC分类号： G01N33/5438 , B82Y30/00 , B82Y40/00 , C01B32/194 , C12Q1/004
摘要： The present invention relates to a biosensor using conductive graphenes and a preparation method thereof, and more particularly, to a biosensor and a preparation method thereof, which uses a conductive graphene prepared using chemical functional groups or a conductive graphine to which a bioreceptor is attached, the bioreceptor being selectively combined to a target biomaterial on a laminated conductive graphene film repeatedly overlaid with the conductive graphenes in order to have a high surface density on a substrate. The conductive graphene biosensor according to the present invention has a large surface area and excellent electric conductivity, and thus can increase the immobilization rate of biological molecules, such as DNA, and enhance the detection sensitivity for biological molecules. In addition, by either directly detecting various target biomolecules or detecting electrochemical signals, the present invention can accurately detect reactions of biomaterials and bioreceptors on a large scale at once, and can introduce a detection method by which an accurate measurement, even of a small amount of source, can be obtained.
摘要翻译：本发明涉及一种使用导电石墨烯的生物传感器及其制备方法，更具体地说，涉及一种生物传感器及其制备方法，该生物传感器及其制备方法使用使用化学官能团或连接有生物受体的导电石墨制备的导电石墨烯，生物受体被选择性地组合到层叠的导电石墨烯膜上的目标生物材料上，所述层压导电石墨烯膜与导电石墨烯重复地重叠以在基底上具有高的表面密度。根据本发明的导电石墨烯生物传感器具有大的表面积和优异的导电性，因此可以增加DNA等生物分子的固定化率，提高生物分子的检测灵敏度。另外，通过直接检测各种目标生物分子或检测电化学信号，本发明可以一次性地大规模地精确地检测生物材料和生物感应器的反应，并且可以引入检测方法，通过该方法进行精确的测量，甚至少量的来源，可以获得。

3. 发明申请

WO2007078127B1 METHOD FOR CELL SURFACE DISPLAYING OF TARGET PROTEINS USING BACILLUS ANTHRACIS EXOSPORIUM 审中-公开
标题翻译：使用BACILLUS ANTHRACIS EXOSPORIUM的目标蛋白的细胞表面显示方法
公开(公告)号：WO2007078127B1
公开(公告)日：2007-12-27
申请号：PCT/KR2006005886
申请日：2006-12-29
申请人： KOREA ADVANCED INST SCI & TECH , LEE SANG YUP , PARK TAE JUNG , HEO NAM SU
发明人： LEE SANG YUP , PARK TAE JUNG , HEO NAM SU
IPC分类号： C12N15/64 , C12N15/63 , C12N15/70
CPC分类号： C07K14/32 , C07K16/00 , C07K2317/622 , C07K2319/00 , C12N15/70 , C12P21/02
摘要： The present invention relates to a method for expressing a target protein on the surface of a microorganism using Bacillus anthracis exosporium protein. More particularly, to an expression vector constructed such that it comprises bclA gene encoding Bacillus anthracis exosporium protein BcIA or fragements thereof as a cell surface anchoring motif and the target protein can be expressed on the surface of a cell in a form fused with BcIA or a fragment thereof when the gene encoding the target protein is expressed in a host cell, as well as, a method for expressing a target protein on the surface of a microorganism using the vector. The expression vector according to the present invention is capable of effectively expressing a target protein or a peptide on the cell surface using BcIA, Bacillus anthracis exosporium protein as a cell surface anchoring motif, and since a target protein can be stably expressed on the cell surface in large amounts by culturing a microorganism transformed with the expression vector, thus making it possible to effectively use for the various purposes of recombinant live vaccines, whole cells absorbents, whole cell bioconversion and the like.
摘要翻译：本发明涉及使用炭疽芽孢杆菌外芽孢蛋白在微生物表面上表达靶蛋白的方法。更具体地说，涉及构建成使得其包含编码作为细胞表面锚定基序的芽孢杆菌属炭疽芽孢杆菌蛋白BcIA或其残基的bclA基因的表达载体，并且靶蛋白可以以与BcIA或BcIA融合的形式在细胞表面表达当在宿主细胞中表达编码靶蛋白的基因时，使用该载体在微生物表面上表达靶蛋白的方法。根据本发明的表达载体能够使用BcIA，炭疽芽孢杆菌外芽蛋白作为细胞表面锚定基序在细胞表面上有效地表达靶蛋白或肽，并且由于靶蛋白可以在细胞表面上稳定表达通过培养用表达载体转化的微生物，可大量使用，从而可有效地用于重组活疫苗，全细胞吸收剂，全细胞生物转化等的各种目的。

4. 发明申请

WO2011037415A3 RECOMBINANT MICROORGANISM WITH INCREASED BUTANOL PRODUCTION ABILITY, AND PREPARATION METHOD OF BUTANOL USING SAME 审中-公开
标题翻译：重组微生物提高丁醇生产能力，以及使用相同的丁醇制备方法
公开(公告)号：WO2011037415A3
公开(公告)日：2011-11-03
申请号：PCT/KR2010006511
申请日：2010-09-21
申请人： KOREA ADVANCED INST SCI & TECH , BIOFUELCHEM CO , GS CALTEX CORP , LEE SANG YUP , JANG YU-SIN , LEE JIN YOUNG , LEE JOUNG MIN , PARK JIN HWAN
发明人： LEE SANG YUP , JANG YU-SIN , LEE JIN YOUNG , LEE JOUNG MIN , PARK JIN HWAN
IPC分类号： C12N1/21 , C12N15/52 , C12P7/16
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/1029 , C12Y101/01001 , C12Y203/01008 , C12Y203/01019 , Y02E50/10
摘要： The present invention relates to a recombinant microorganism with an increased butanol production ability, and a preparation method of butanol using the same, and more specifically, to a recombinant microorganism of which the butanol production ability is increased by the manipulation of metabolic pathways, and a preparation method of butanol using the same. The recombinant microorganism with an increased butanol production ability according to the present invention is characterized in that a gene encoding an enzyme which converts acetyl CoA into acetate is deleted in a host microorganism having a gene encoding enzymes involved in the biosynthetic pathway into acetyl CoA and butyryl CoA. Since the recombinant microorganism according to the present invention obtained by the manipulation of the metabolic flow of a microorganism can selectively produce butanol with a high efficiency, it is useful as a microorganism producing an industrial solvent and a transportation fuel.
摘要翻译：本发明涉及丁醇生产能力提高的重组微生物，以及使用其的丁醇的制备方法，更具体地说，涉及通过代谢途径的操作增加丁醇生产能力的重组微生物，以及丁醇的制备方法。根据本发明的具有增加的丁醇生产能力的重组微生物的特征在于编码将乙酰CoA转化为乙酸的酶的基因在具有编码参与生物合成途径的酶的基因的宿主微生物中缺失为乙酰辅酶A和丁酰基辅酶。由于通过操作微生物的代谢流而获得的根据本发明的重组微生物可以选择性地高效地生产丁醇，因此它可用作生产工业溶剂和运输燃料的微生物。

5. 发明申请

WO2010093150A3 RECOMBINANT MICROORGANISM WITH ENHANCED ABILITY FOR GLYCEROL METABOLISM AND SUCCINIC ACID PRODUCTION, AND METHOD FOR PRODUCING SUCCINIC ACID USING SAME 审中-公开
标题翻译：具有增加甘油代谢和产酸生产能力的重组微生物，以及使用其制备磺酸的方法
公开(公告)号：WO2010093150A3
公开(公告)日：2010-12-09
申请号：PCT/KR2010000755
申请日：2010-02-08
申请人： KOREA ADVANCED INST SCI & TECH , LEE SANG YUP , JEON YONG JAE , SONG HYOHAK
发明人： LEE SANG YUP , JEON YONG JAE , SONG HYOHAK
IPC分类号： C12N15/31 , C12N1/21
CPC分类号： C12P7/46
摘要： The present invention relates to a recombinant microorganism with enhanced ability for glycerol metabolism and succinic acid production, and to a method for producing succinic acid using same. More particularly, the present invention relates to a recombinant microorganism which is enhanced to produce a high concentration succinic acid by effectively using inexpensive glycerol as a carbon source for cells. The recombinant microorganism according to the present invention enables succinic acid, which is a valuable substance produced by conventional chemical processes, to be produced through production-efficient and environmentally-friendly biological processes, thus reducing the costs of producing the target metabolite. Further, the recombinant microorganism according to the present invention is advantageous in that 100% pure succinic acid can be produced without forming byproducts, as opposed to conventional techniques in which only a slight reduction of byproducts such as acetic acid, lactic acid, formic acid, pyruvic acid, etc., is achieved.
摘要翻译：本发明涉及增加甘油代谢和琥珀酸生产能力的重组微生物，以及使用其生产琥珀酸的方法。更具体地，本发明涉及通过有效地使用廉价的甘油作为细胞的碳源来增强生产高浓度琥珀酸的重组微生物。根据本发明的重组微生物使得通过常规化学方法生产的有价值物质的琥珀酸通过生产有效和环境友好的生物过程产生，从而降低生产目标代谢物的成本。此外，根据本发明的重组微生物的优点在于，与常规技术相反，可以生产100％纯的琥珀酸而不形成副产物，其中只有轻微降低副产物如乙酸，乳酸，甲酸，丙酮酸等。

6. 发明申请

WO2009157702A3 METHOD FOR PREPARING POLYLACTATE AND COPOLYMER THEREOF USING A MUTANT MICROORGANISM WITH ENHANCED POLYLACTATE, AND THE COPOLYMER PRODUCING CAPABILITY THEREOF 审中-公开
标题翻译：使用具有增强聚氨酯的突变微生物制备聚乙烯和共聚物的方法，以及其生产能力的共聚物
公开(公告)号：WO2009157702A3
公开(公告)日：2010-04-22
申请号：PCT/KR2009003389
申请日：2009-06-24
申请人： LG CHEMICAL LTD , KOREA ADVANCED INST SCI & TECH , YANG TAEK HO , PARK SI JAE , KIM TAE WAN , LEE SANG YUP , JUNG YU KYUNG
发明人： YANG TAEK HO , PARK SI JAE , KIM TAE WAN , LEE SANG YUP , JUNG YU KYUNG
IPC分类号： C08G63/91
CPC分类号： C08G63/06 , C12P7/625 , C12P21/02
摘要： The present invention relates to a method for preparing a polylactate and a copolymer thereof using a mutant microorganism in which a gene involving a coenzyme A donor and lactate producing pathway is genetically manipulated to increase the productivity of the coenzyme A donor and lactate. According to the present invention, the amount of coenzyme A donor and the amount of lactate are simultaneously increased in a microbial metabolic pathway, to thereby enable an efficient biosynthesis of a polylactate and a hydroxyalkanoate-lactate copolymer containing a high content of lactate, thus making the method of the present invention industrially useful.
摘要翻译：本发明涉及一种使用突变微生物制备聚乳酸及其共聚物的方法，其中遗传操作涉及辅酶A供体和乳酸生产途径的基因以提高辅酶A供体和乳酸盐的生产率。根据本发明，在微生物代谢途径中同时增加辅酶A供体的量和乳酸的量，从而能够有效地生物合成含有高含量乳酸的聚乳酸和羟基链烷酸酯 - 乳酸共聚物，从而使本发明的方法在工业上有用。

7. 发明申请

WO2012124941A3 RECOMBINANT MICROORGANISM HAVING ABILITY TO PRODUCE POLYHYDROXYALKANOATE CONTAINING 2-HYDROXYBUTYRATE AS MONOMER, AND METHOD FOR PREPARING POLYHYDROXYALKANOATE CONTAINING 2-HYDROXYBUTYRATE AS MONOMER USING SAME 审中-公开
标题翻译：具有生产含有2-羟基丁酸酯的聚羟基烷酸酯作为单体的重组微生物以及制备含有2-羟基丁酸酯的聚羟基己酸酯作为单体的方法
公开(公告)号：WO2012124941A3
公开(公告)日：2012-12-27
申请号：PCT/KR2012001752
申请日：2012-03-09
申请人： KOREA ADVANCED INST SCI & TECH , KOREA RES INST CHEM TECH , LEE SANG YUP , PARK SI JAE , LEE SEUNG HWAN , SONG BONG KEUN , LEE TAE WOO
发明人： LEE SANG YUP , PARK SI JAE , LEE SEUNG HWAN , SONG BONG KEUN , LEE TAE WOO
IPC分类号： C12N1/21 , C12N15/52 , C12P7/62
CPC分类号： C12N9/1025 , C12N9/13 , C12P7/625
摘要： The present invention relates to a polyhydroxyalkanoate containing a 2-hydroxybutyrate as a monomer, a recombinant microorganism having the ability to produce the polyhydroxyalkanoate containing the 2-hydroxybutyrate as the monomer, and a method for preparing the polyhydroxyalkanoate containing the 2-hydroxybutyrate as the monomer. The present invention can effectively provide the polyhydroxyalkanoate containing the 2-hydroxybutyrate, which is a novel biodegradable polymer, and the recombinant microorganism for producing same.
摘要翻译：本发明涉及含有2-羟基丁酸酯作为单体的聚羟基链烷酸酯，具有生产含有2-羟基丁酸酯作为单体的聚羟基链烷酸酯的能力的重组微生物以及制备含有2-羟基丁酸酯作为单体的聚羟基链烷酸酯的方法。本发明可以有效地提供含有作为新型生物可降解聚合物的2-羟基丁酸酯的聚羟基链烷酸酯和用于生产它们的重组微生物。

8. 发明申请

WO2012124941A2 RECOMBINANT MICROORGANISM HAVING ABILITY TO PRODUCE POLYHYDROXYALKANOATE CONTAINING 2-HYDROXYBUTYRATE AS MONOMER, AND METHOD FOR PREPARING POLYHYDROXYALKANOATE CONTAINING 2-HYDROXYBUTYRATE AS MONOMER USING SAME 审中-公开
标题翻译：具有生产含有2-羟基丁酸酯的聚羟基烷基酯作为单体的能力的重组微生物以及使用它们制备含有2-羟基丁酸酯的聚羟基烷基酯作为单体的方法
公开(公告)号：WO2012124941A2
公开(公告)日：2012-09-20
申请号：PCT/KR2012001752
申请日：2012-03-09
申请人： KOREA ADVANCED INST SCI & TECH , KOREA RES INST CHEM TECH , LEE SANG YUP , PARK SI JAE , LEE SEUNG HWAN , SONG BONG KEUN , LEE TAE WOO
发明人： LEE SANG YUP , PARK SI JAE , LEE SEUNG HWAN , SONG BONG KEUN , LEE TAE WOO
IPC分类号： C12N1/21 , C12N15/52 , C12P7/62
CPC分类号： C12N9/1025 , C12N9/13 , C12P7/625
摘要： The present invention relates to a polyhydroxyalkanoate containing a 2-hydroxybutyrate as a monomer, a recombinant microorganism having the ability to produce the polyhydroxyalkanoate containing the 2-hydroxybutyrate as the monomer, and a method for preparing the polyhydroxyalkanoate containing the 2-hydroxybutyrate as the monomer. The present invention can effectively provide the polyhydroxyalkanoate containing the 2-hydroxybutyrate, which is a novel biodegradable polymer, and the recombinant microorganism for producing same.
摘要翻译：本发明涉及含有2-羟基丁酸酯作为单体的聚羟基链烷酸酯，具有生产含有2-羟基丁酸酯作为单体的聚羟基链烷酸酯的能力的重组微生物，以及制备含有2-羟基丁酸酯作为单体的聚羟基链烷酸酯的方法。本发明可以有效地提供含有作为新型生物降解性聚合物的2-羟基丁酸酯的聚羟基链烷酸酯及其制造用重组微生物。

9. 发明申请

WO2012033334A3 RECOMBINANT MICROORGANISM HAVING AN IMPROVED ABILITY TO PRODUCE BUTANOL AND A PRODUCTION METHOD FOR BUTANOL USING THE SAME 审中-公开
标题翻译：具有改进的生产丁醇能力的重组微生物以及使用该生产方法生产丁醇的方法
公开(公告)号：WO2012033334A3
公开(公告)日：2012-08-23
申请号：PCT/KR2011006607
申请日：2011-09-07
申请人： KOREA ADVANCED INST SCI & TECH , LEE SANG YUP , CHOI YONG JUN , PARK JIN HWAN
发明人： LEE SANG YUP , CHOI YONG JUN , PARK JIN HWAN
IPC分类号： C12N1/21 , C12N15/52 , C12P7/16
CPC分类号： C12Y102/07007 , C12N9/0008 , C12P7/16 , C12P19/32 , Y02E50/10
摘要： The present invention relates to a recombinant microorganism having an improved ability to produce butanol and to a production method for butanol using the same, and more specifically relates to a recombinant microorganism for biosynthesis of butanol in which 2-ketoisovalerate, which is a precursor of L-valine, is used as an intermediate, and relates to a production method for butanol using the same. A feature of the recombinant microorganism according to the present invention is that a microorganism having the ability to produce 2-ketoisovalerate, which is a precursor of L-valine, has introduced therein: a gene coding for VorABC enzyme which is an enzyme that converts 2-ketoisovalerate into isobutyryl-CoA; a gene coding for an enzyme that converts isobutyryl-CoA into butyryl-CoA; a gene coding for an enzyme that converts butyryl-CoA into butyraldehyde; and a gene coding for an enzyme that converts butyraldehyde into butanol. Unlike existing Clostridium acetobutylicum, the recombinant microorganism according to the present invention is easy to grow, has a substantial strain-improvement potential due to additional metabolic flux manipulation and is useful as a microorganism for the industrial production of butanol, and a recombinant microorganism is provided which has an improved ability to produce butanol by simplifying the process for converting 2-ketoisovalerate to isobutyryl-CoA.
摘要翻译：本发明涉及丁醇生产能力提高的重组微生物以及使用其的丁醇生产方法，并且更具体地涉及用于生物合成丁醇的重组微生物，其中2-酮异戊酸盐是L - 缬氨酸用作中间体，并且涉及使用其的丁醇的生产方法。根据本发明的重组微生物的特征在于具有产生L-缬氨酸的前体2-酮异戊酸的能力的微生物已经引入其中：编码VorABC酶的基因，所述VorABC酶是将2位 - 酮异戊酸化为异丁酰-CoA; 编码将异丁酰-CoA转化为丁酰-CoA的酶的基因; 编码将丁酰-CoA转化为丁醛的酶的基因; 和编码将丁醛转化为丁醇的酶的基因。与现有的丙酮丁醇梭菌不同，本发明的重组微生物容易生长，由于额外的代谢通量操作而具有显着的菌株改善潜力，并且可用作丁醇工业生产的微生物，并且提供了重组微生物其通过简化将2-酮异戊酸转化成异丁酰-CoA的过程而具有改进的生产丁醇的能力。

10. 发明申请

WO2011034397A2 METHOD FOR PREDICTING DRUG TARGETS AND SCREENING FOR DRUGS FOR PATHOGENIC MICROORGANISMS USING ESSENTIAL METABOLITES 审中-公开
标题翻译：用于预防药物靶标的方法和使用基本代谢物的病原微生物药物筛选
公开(公告)号：WO2011034397A2
公开(公告)日：2011-03-24
申请号：PCT/KR2010006469
申请日：2010-09-20
申请人： KOREA ADVANCED INST SCI & TECH , LEE SANG YUP , KIM HYUN UK , KIM TAE YONG , YI KYU YANG
发明人： LEE SANG YUP , KIM HYUN UK , KIM TAE YONG , YI KYU YANG
IPC分类号： C12Q1/04 , C12Q1/00
CPC分类号： G06F19/12
摘要： The present invention relates to a method for predicting drug targets and screening for drugs for pathogenic microorganisms using essential metabolites. More particularly, the present invention relates to a method for screening for efficient drug target enzymes for pathogenic microorganisms or for drug target genes encoding the enzymes, and to a method for screening for drugs capable of inhibiting the growth of pathogenic microorganisms, wherein said methods comprise: selecting target microorganisms; constructing a metabolism network model for the selected microorganisms; predicting the metabolite that is essential for cell growth using a metabolite essentiality analysis; deleting a currency metabolite, and deleting an essential metabolite, the number of outgoing reactions of which is less than a reference number; and selecting the remaining essential metabolites and essential metabolites such that enzymes which consume the essential metabolites are not present in the metabolism of a host.
摘要翻译：本发明涉及使用必需代谢物预测药物靶标和筛选病原微生物药物的方法。更具体地说，本发明涉及用于筛选用于病原微生物的有效药物靶酶或编码酶的药物靶基因的方法，以及用于筛选能够抑制病原微生物生长的药物的方法，其中所述方法包括：选择目标微生物; 构建所选微生物的代谢网络模型; 使用代谢物本质分析预测细胞生长所必需的代谢物; 删除货币代谢物，删除必需代谢物，其输出反应数小于参考数; 并选择剩余的必需代谢物和必需代谢物，使得消耗主要代谢物的酶不存在于宿主代谢中。

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