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1. 发明申请

WO2007078127B1 METHOD FOR CELL SURFACE DISPLAYING OF TARGET PROTEINS USING BACILLUS ANTHRACIS EXOSPORIUM 审中-公开
标题翻译：使用BACILLUS ANTHRACIS EXOSPORIUM的目标蛋白的细胞表面显示方法
公开(公告)号：WO2007078127B1
公开(公告)日：2007-12-27
申请号：PCT/KR2006005886
申请日：2006-12-29
申请人： KOREA ADVANCED INST SCI & TECH , LEE SANG YUP , PARK TAE JUNG , HEO NAM SU
发明人： LEE SANG YUP , PARK TAE JUNG , HEO NAM SU
IPC分类号： C12N15/64 , C12N15/63 , C12N15/70
CPC分类号： C07K14/32 , C07K16/00 , C07K2317/622 , C07K2319/00 , C12N15/70 , C12P21/02
摘要： The present invention relates to a method for expressing a target protein on the surface of a microorganism using Bacillus anthracis exosporium protein. More particularly, to an expression vector constructed such that it comprises bclA gene encoding Bacillus anthracis exosporium protein BcIA or fragements thereof as a cell surface anchoring motif and the target protein can be expressed on the surface of a cell in a form fused with BcIA or a fragment thereof when the gene encoding the target protein is expressed in a host cell, as well as, a method for expressing a target protein on the surface of a microorganism using the vector. The expression vector according to the present invention is capable of effectively expressing a target protein or a peptide on the cell surface using BcIA, Bacillus anthracis exosporium protein as a cell surface anchoring motif, and since a target protein can be stably expressed on the cell surface in large amounts by culturing a microorganism transformed with the expression vector, thus making it possible to effectively use for the various purposes of recombinant live vaccines, whole cells absorbents, whole cell bioconversion and the like.
摘要翻译：本发明涉及使用炭疽芽孢杆菌外芽孢蛋白在微生物表面上表达靶蛋白的方法。更具体地说，涉及构建成使得其包含编码作为细胞表面锚定基序的芽孢杆菌属炭疽芽孢杆菌蛋白BcIA或其残基的bclA基因的表达载体，并且靶蛋白可以以与BcIA或BcIA融合的形式在细胞表面表达当在宿主细胞中表达编码靶蛋白的基因时，使用该载体在微生物表面上表达靶蛋白的方法。根据本发明的表达载体能够使用BcIA，炭疽芽孢杆菌外芽蛋白作为细胞表面锚定基序在细胞表面上有效地表达靶蛋白或肽，并且由于靶蛋白可以在细胞表面上稳定表达通过培养用表达载体转化的微生物，可大量使用，从而可有效地用于重组活疫苗，全细胞吸收剂，全细胞生物转化等的各种目的。

2. 发明申请

WO2011021846A2 MULTI-SPOT METAL-DEPOSITED NUCLEIC ACID CHIP WITH NANOSTRUCTURE ARRAYS FOR DIAGNOSING CORNEAL DYSTROPHY, AND METHOD FOR PRODUCING SAME 审中-公开
标题翻译：纳米结构阵列用于诊断角膜营养不良的多点金属沉积的核酸芯片及其制备方法
公开(公告)号：WO2011021846A2
公开(公告)日：2011-02-24
申请号：PCT/KR2010005452
申请日：2010-08-18
申请人： KOREA ADVANCED INST SCI & TECH , AVELLINO CO LTD , LEE SANG YUP , YOO SO YOUNG , KIM DO KYUN , PARK TAE JUNG , YUN JUNG KUK , LEE GENE
发明人： LEE SANG YUP , YOO SO YOUNG , KIM DO KYUN , PARK TAE JUNG , YUN JUNG KUK , LEE GENE
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , B01J2219/00509 , B01J2219/00608 , B01J2219/00612 , B01J2219/00614 , B01J2219/00621 , B01J2219/00637 , B01J2219/00648 , B01J2219/00722 , B82Y30/00 , C12Q1/6883 , C12Q2600/158
摘要： The present invention relates to a multi-spot metal-deposited nucleic acid chip with nanostructure arrays for diagnosing corneal dystrophy, and more particularly, to a multi-spot metal-deposited nucleic acid chip with nanostructure arrays which uses the optical characteristics of localized surface plasmon resonance (LSPR), to a method for producing the chip, and to a multi-spot metal-deposited nucleic acid chip with nanostructure arrays for diagnosing BIGH3 gene mutations and various types of corneal dystrophies. According to the present invention, the metal-deposited nucleic acid chip with nanostructure arrays, and an analysis device including a light source, a detector, a spectrophotometer and a computer are combined to be used as a label-free biosensor based on the optical characteristics of LSPR. The multi-spot metal-deposited nucleic acid chip with nanostructure arrays for diagnosing BIGH3 gene mutations and corneal dystrophy according to the present invention diagnoses various types of corneal dystrophies, which are hereditary eye diseases, at the same time.
摘要翻译：具有纳米结构阵列的多点金属沉积核酸芯片技术领域本发明涉及用于诊断角膜营养不良的具有纳米结构阵列的多点金属沉积核酸芯片，并且更具体地涉及具有纳米结构阵列的多点金属沉积核酸芯片，其使用局部表面等离子体共振（LSPR），用于生产芯片的方法，以及具有纳米结构阵列的多点金属沉积核酸芯片，用于诊断BIGH3基因突变和各种类型的角膜营养不良。根据本发明，将具有纳米结构阵列的金属沉积核酸芯片和包括光源，检测器，分光光度计和计算机的分析装置组合以用作基于光学特性的无标记生物传感器的LSPR。根据本发明的用于诊断BIGH3基因突变和角膜营养不良的具有纳米结构阵列的多点金属沉积核酸芯片同时诊断作为遗传性眼病的各种类型的角膜营养不良。

3. 发明申请

WO2009157739A3 BIOSENSOR USING CONDUCTIVE GRAPHENES AND PREPARATION METHOD THEREOF 审中-公开
标题翻译：使用导电石墨的生物传感器及其制备方法
公开(公告)号：WO2009157739A3
公开(公告)日：2010-04-01
申请号：PCT/KR2009003482
申请日：2009-06-26
申请人： KOREA ADVANCED INST SCI & TECH , LEE SANG YUP , PARK TAE JUNG , HONG WON HI , JUNG HEE TAE , PARK HO SEOK , CHOI BONG GILL
发明人： LEE SANG YUP , PARK TAE JUNG , HONG WON HI , JUNG HEE TAE , PARK HO SEOK , CHOI BONG GILL
IPC分类号： C01B31/02
CPC分类号： G01N33/5438 , B82Y30/00 , B82Y40/00 , C01B32/194 , C12Q1/004
摘要： The present invention relates to a biosensor using conductive graphenes and a preparation method thereof, and more particularly, to a biosensor and a preparation method thereof, which uses a conductive graphene prepared using chemical functional groups or a conductive graphine to which a bioreceptor is attached, the bioreceptor being selectively combined to a target biomaterial on a laminated conductive graphene film repeatedly overlaid with the conductive graphenes in order to have a high surface density on a substrate. The conductive graphene biosensor according to the present invention has a large surface area and excellent electric conductivity, and thus can increase the immobilization rate of biological molecules, such as DNA, and enhance the detection sensitivity for biological molecules. In addition, by either directly detecting various target biomolecules or detecting electrochemical signals, the present invention can accurately detect reactions of biomaterials and bioreceptors on a large scale at once, and can introduce a detection method by which an accurate measurement, even of a small amount of source, can be obtained.
摘要翻译：本发明涉及一种使用导电石墨烯的生物传感器及其制备方法，更具体地说，涉及一种生物传感器及其制备方法，该生物传感器及其制备方法使用使用化学官能团或连接有生物受体的导电石墨制备的导电石墨烯，生物受体被选择性地组合到层叠的导电石墨烯膜上的目标生物材料上，所述层压导电石墨烯膜与导电石墨烯重复地重叠以在基底上具有高的表面密度。根据本发明的导电石墨烯生物传感器具有大的表面积和优异的导电性，因此可以增加DNA等生物分子的固定化率，提高生物分子的检测灵敏度。另外，通过直接检测各种目标生物分子或检测电化学信号，本发明可以一次性地大规模地精确地检测生物材料和生物感应器的反应，并且可以引入检测方法，通过该方法进行精确的测量，甚至少量的来源，可以获得。

4. 发明公开

EP2468884A4 MULTI-SPOT METAL-DEPOSITED NUCLEIC ACID CHIP WITH NANOSTRUCTURE ARRAYS FOR DIAGNOSING CORNEAL DYSTROPHY, AND METHOD FOR PRODUCING SAME 有权转让
标题翻译：与金属矿床有几点和纳米结构的阵列用于诊断角膜营养不良，及其制造方法NUCLEIC CHIP
公开(公告)号：EP2468884A4
公开(公告)日：2016-02-17
申请号：EP10810154
申请日：2010-08-18
申请人： KOREA ADVANCED INST SCI & TECH , AVELLINO CO LTD
发明人： LEE SANG YUP , YOO SO YOUNG , KIM DO KYUN , PARK TAE JUNG , YUN JUNG KUK , LEE GENE
IPC分类号： C12Q1/68 , B01J19/00 , B82Y15/00 , B82Y30/00
CPC分类号： C12Q1/6837 , B01J2219/00509 , B01J2219/00608 , B01J2219/00612 , B01J2219/00614 , B01J2219/00621 , B01J2219/00637 , B01J2219/00648 , B01J2219/00722 , B82Y30/00 , C12Q1/6883 , C12Q2600/158

5. 发明公开

EP2021480A4 METHOD FOR PREPARING METAL NANOPARTICLE USING METAL BINDING PROTEIN 审中-公开
标题翻译：一种用于生产金属纳米粒子使用金属结合蛋白
公开(公告)号：EP2021480A4
公开(公告)日：2010-10-20
申请号：EP07746028
申请日：2007-04-17
申请人： KOREA ADVANCED INST SCI & TECH
发明人： LEE SANG YUP , PARK TAE JUNG
IPC分类号： C12N9/10 , C07K14/825 , C12P3/00 , G01N33/531 , G01N33/58
CPC分类号： C07K14/825 , B82Y5/00 , B82Y15/00 , C12N9/104 , C12P3/00 , G01N33/531 , G01N33/588 , Y10T428/2982

6. 发明专利

DE112006003608T5 未知
公开(公告)号：DE112006003608T5
公开(公告)日：2008-10-30
申请号：DE112006003608
申请日：2006-12-29
申请人： KOREA ADVANCED INST SCI & TECH
发明人： LEE SANG YUP , PARK TAE JUNG , HEO NAM SU
IPC分类号： C12N15/64 , C12N15/63 , C12N15/70

7. 发明专利

AU2003289568A1 METHOD FOR WHOLE SURROUNDING SURFACE DISPLAY OF TARGET PROTEINS USING BACTERIAL EXOSPORIUM 未知
公开(公告)号：AU2003289568A1
公开(公告)日：2005-04-11
申请号：AU2003289568
申请日：2003-12-30
申请人： KOREA ADVANCED INST SCI & TECH
发明人： LEE SANG YUP , PARK TAE JUNG , PARK JONG PIL , LEE SEOK JAE
IPC分类号： C12N15/63 , C12N15/10 , C40B40/02 , C12N1/20 , A61K49/16 , C12N15/66
摘要： The present invention relates to a method for expressing a target protein on an exosporium forming the outermost surface of bacterial spores. More particularly, the present invention relates to a method for expressing a target protein on the surface of cells and spores using an exosporium as a matrix for surface expression, and methods for the production of a protein array, the production of antibodies, the separation of a certain substance from a mixture, bioconversion, and the improvement of a target protein, which are characterized by using the cells or spores having the target protein that was expressed on the surface by the above expression method. The method for expressing the target protein on the surface of the spore outer membrane of the gene carriers according to the present invention has effects in that a variety of the target proteins can be expressed and the level of surface expression of the target protein is increased compared to the existing technology, and also the structural stability of the gene carriers having the target protein expressed on their surface, the viability of the host, and the rapidity of the screening method, are greatly increased.

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