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1. 发明授权

US5362916A Synthesis of mercaptaryl or hydroxyaryl enol ether alkali metal salts 失效
标题翻译：硫醇芳基或羟基芳基烯醇醚碱金属盐的合成
公开(公告)号：US5362916A
公开(公告)日：1994-11-08
申请号：US153791
申请日：1993-11-17
申请人： Brooks Edwards , Rouh-Rong Juo
发明人： Brooks Edwards , Rouh-Rong Juo
IPC分类号： C07C43/174 , C07C45/29 , C07C45/71 , C07C47/575 , C07D321/00 , C07F9/40 , C07F9/655 , C07F9/6561 , G01N21/76 , C07C49/167
CPC分类号： C07F9/6561 , C07C43/1747 , C07C45/292 , C07C45/71 , C07C47/575 , C07D321/00 , C07F9/4056 , C07F9/65512 , C07F9/65517 , C07F9/65522 , C07C2103/74
摘要： Hydroxyaryl enol ether alkali metal salts having the formula: ##STR1## in which T is a fused, substituted or unsubstituted polycycloalkylidene group, OR.sup.3 is an ether group Y is a light-emitting fluorophore-forming group which will be part of a luminescent substance formed by decomposition of a 1,2-dioxetane subsequently formed from the hydroxyaryl enol ether alkali metal salt, capable of absorbing energy to form an excited state from which it emits optically detectable energy to return to its ground state, and AM.sup.30 is an alkali metal cation, processes for the preparation of these intermediate salts, and their use as starting materials for acylation, phosphorylation and glycosylation reactions to give intermediates reactable to give stable, water-soluble chemiluminescent 1,2-dioketames, particularly ones that are enzymatically cleavable, are disclosed.
摘要翻译：具有下式的羟基芳基烯醇醚碱金属盐：其中T是稠合的，取代或未取代的多亚烷基，OR 3是醚基，Y是作为形成的发光物质的一部分的发光荧光团形成基团通过分解随后由羟基芳基烯醇醚碱金属盐形成的1,2-二氧杂环丁烷，其能够吸收能量以形成激发态，从其发射光学上可检测的能量以返回其基态，并且AM30是碱金属阳离子，这些中间体盐的制备方法，以及它们作为酰化，磷酸化和糖基化反应的起始原料的用途，得到可反应的中间体，得到稳定的水溶性化学发光1,2-二酮甲酮，特别是可酶切割的化合物。。

2. 发明授权

US4876399A Taxols, their preparation and intermediates thereof 失效
标题翻译：紫杉醇，其制备方法及其中间体
公开(公告)号：US4876399A
公开(公告)日：1989-10-24
申请号：US115665
申请日：1987-11-02
申请人： Robert A. Holton , Rouh-Rong Juo , Richard Lowenthal
发明人： Robert A. Holton , Rouh-Rong Juo , Richard Lowenthal
IPC分类号： C07C35/37 , C07C233/47 , C07C233/49 , C07C233/87 , C07F7/18
CPC分类号： C07C233/47 , C07C233/49 , C07C233/87 , C07C35/37 , C07F7/1856 , C07C2103/86 , C07C2103/90
摘要： The present invention is directed to novel taxols useful as a chemotherapeutic agent. Moreover, the present invention is directed to the process of preparing taxols and various intermediates in said process. A key intermediate is this process is 2,5-dihydroxy-2-patchoulenes. Therefore the present invention is also related to said intermediate and the process for its preparation.
摘要翻译：本发明涉及可用作化学治疗剂的新型紫杉醇。此外，本发明涉及在所述方法中制备紫杉醇和各种中间体的方法。该过程的关键中间体是2,5-二羟基-2-肉桂酸。因此，本发明也涉及所述中间体及其制备方法。

3. 发明授权

US5831102A Chemiluminescent 3-(substituted adamant-2'-ylidene) 1,2-dioxetanes 失效
公开(公告)号：US5831102A
公开(公告)日：1998-11-03
申请号：US598353
申请日：1996-02-08
申请人： Irena Y. Bronstein , Brooks Edwards , Rouh-Rong Juo
发明人： Irena Y. Bronstein , Brooks Edwards , Rouh-Rong Juo
IPC分类号： C07D321/00 , C12Q1/68
CPC分类号： C07F9/12 , C07F9/6551 , C09K11/07 , Y10S435/81 , Y10S436/80 , Y10S436/808
摘要： Enzymatically cleavable chemiluminescent 1,2-dioxetane compounds capable of producing light energy when decomposed, substantially stable at room temperature before a bond by which an enzymatically cleavable labile substituent thereof is intentionally cleaved, are disclosed. These compounds can be represented by the formula: ##STR1## wherein: X and X.sup.1 each represent, individually, hydrogen, a hydroxyl group, a halo substituent, an unsubstituted lower alkyl group, a hydroxy (lower) alkyl group, a halo (lower) alkyl group, a phenyl group, a halophenyl group, an alkoxyphenyl group, a hydroxyalkoxy group, a cyano group or an amide group, with at least one of X and X.sup.1 being other than hydrogen; and R.sub.1 and R.sub.2, individually or together, represent an organic substituent that does not interfere with the production of light when the dioxetane compound is enzymatically cleaved and that satisfies the valence of the dioxetane compound's 4-carbon atom, with the provisos that if R.sup.1 and R.sub.2 represent individual substituents the R.sub.2 substituent is aromatic, heteroaromatic, or an unsaturated substituent in conjugation with an aromatic ring, and that at least one of R.sub.1 and R.sub.2 is, or R.sub.1 and R.sub.2 taken together are, an enzymatically cleavable labile group-substituted fluorescent chromophore group that produces a luminescent substance when the enzymatically cleavable labile substituent thereof is removed by an enzyme. The corresponding dioxetanes which, instead of being substituted at the 5' or 7', or at the 5' and 7' positions, instead contain a 4' methylene group, are also disclosed, as are intermediates for all these 3-substituted adamant-2'-ylidenedioxetanes, and their use as reporter molecules in assays.

4. 发明授权

US5543295A Chemiluminescent 3--(Substituted adamant--2'--Ylidene) 1,2--Dioxetanes 失效
标题翻译：化学发光3-（取代的金刚烷-2'-亚基）1,2-二氧杂环丁烷
公开(公告)号：US5543295A
公开(公告)日：1996-08-06
申请号：US233085
申请日：1994-04-25
申请人： Irena Y. Bronstein , Brooks Edwards , Rouh-Rong Juo
发明人： Irena Y. Bronstein , Brooks Edwards , Rouh-Rong Juo
IPC分类号： C07C43/178 , C07C43/196 , C07C43/215 , C07C69/00 , C07D321/00 , C07F9/12 , C07F9/655 , C07F9/6574 , C07H15/203 , C07H15/26 , C12Q1/34 , G01N21/76 , G01N33/58 , C12Q1/68
CPC分类号： C07H15/203 , C07C43/1788 , C07C43/196 , C07C43/215 , C07C69/00 , C07D321/00 , C07F9/12 , C07F9/65512 , C07F9/65742 , C07H15/26 , C12Q1/34 , G01N33/581 , G01N33/582 , C07C2103/00 , C07C2103/74 , Y10S435/81 , Y10S435/968 , Y10S436/80 , Y10S436/808
摘要： Enzymatically clearable chemiluminescent 1,2-dioxetane compounds capable of producing light energy when decomposed, substantially stable at room temperature before a bond by which an enzymatically clearable labile substituent thereof is intentionally cleaved, are disclosed. These compounds can be represented by the formula: ##STR1## wherein: X and X.sup.1 each represent, individually, hydrogen, a hydroxyl group, a halo substituent, an unsubstituted lower alkyl group, a hydroxy (lower) alkyl group, a halo (lower) alkyl group, a phenyl group, a halophenyl group, an alkoxyphenyl group, a hydroxyalkoxy group, a cyano group or an amide group, with at least one of X and X.sup.1 being other than hydrogen; and R.sub.1 and R.sub.2, individually or together, represent an organic substituent that does not interfere with the production of light when the dioxetane compound is enzymatically cleaved and that satisfies the valence of the dioxetane compound's 4-carbon atom, with the provisos that if R.sub.1 and R.sub.2 represent individual substituents the R.sub.2 substituent is aromatic, heteroaromatic, or an unsaturated substituent in conjugation with an aromatic ring, and that at least one of R.sub.1 and R.sub.2 is, or R.sub.1 and R.sub.2 taken together are, an enzymatically cleavable labile group-substituted fluorescent chromophore group that produces a luminenscent substance when the enzymatically cleavable labile substituent thereof is removed by an enzyme. The corresponding dioxetanes which, instead of being substituted at the 5' or 7', or at the 5' and 7' positions, instead contain a 40' mehtylene group, are also disclosed, as are intermediates for all these 3-substituted adamant-2'-ylidenedioxetanes, and their use as reporter molecules in assays.
摘要翻译：公开了能够在分解时产生光能的化学发光化学发光的1,2-二氧杂环丁烷化合物，其在可消除其可酶切的不稳定取代基被有意地切割的键之前在室温下基本稳定。这些化合物可以由下式表示：其中：X和X1各自独立地表示氢，羟基，卤素取代基，未取代的低级烷基，羟基（低级）烷基，卤代（低级））烷基，苯基，卤代苯基，烷氧基苯基，羟基烷氧基，氰基或酰胺基，其中X和X 1中的至少一个不是氢; 并且R 1和R 2各自或一起表示当二氧杂环丁烷化合物被酶切并且满足二恶丁环化合物的4-碳原子的化合价时不干扰光的产生的有机取代基，条件是如果R1和 R2代表单个取代基，R2取代基是芳族的，杂芳族的，或与芳环共轭的不饱和取代基，并且R1和R2中的至少一个是，或者R1和R2一起是可酶切的不稳定基取代的荧光当其酶促切割的不稳定取代基被酶除去时产生光敏物质的发色团。还公开了相应的二氧杂环丁烷，而不是在5'或7'或5'和7'位置被取代，而是含有40'甲基，也可以是所有这些3-取代的金刚烷基 - 2'-亚基二氧杂环丁烷，以及它们在测定中作为报道分子的用途。

5. 发明授权

US5330900A Chemiluminescent 3-(substituted adamant-2'-ylidene) 1,2-dioxetanes 失效
标题翻译：化学发光3-（取代的金刚烷-2'-亚基）1,2-二氧杂环丁烷
公开(公告)号：US5330900A
公开(公告)日：1994-07-19
申请号：US806928
申请日：1991-12-12
申请人： Irena Y. Bronstein , Brooks Edwards , Rouh-Rong Juo
发明人： Irena Y. Bronstein , Brooks Edwards , Rouh-Rong Juo
IPC分类号： C07C43/178 , C07C43/196 , C07C43/215 , C07C69/00 , C07D321/00 , C07F9/12 , C07F9/655 , C07F9/6574 , C07H15/203 , C07H15/26 , C12Q1/34 , G01N21/76 , G01N33/58 , C12Q1/68
CPC分类号： C07H15/203 , C07C43/1788 , C07C43/196 , C07C43/215 , C07C69/00 , C07D321/00 , C07F9/12 , C07F9/65512 , C07F9/65742 , C07H15/26 , C12Q1/34 , G01N33/581 , G01N33/582 , C07C2103/00 , C07C2103/74 , Y10S435/81 , Y10S435/968 , Y10S436/80 , Y10S436/808
摘要： Enzymatically cleavable chemiluminescent 1,2-dioxetane compounds capable of producing light energy when decomposed, substantially stable at room temperature before a bond by which an enzymatically cleavable labile substituent thereof is intentionally cleaved, are disclosed. These compounds can be represented by the formula: ##STR1## wherein: X and X.sup.1 each represent, individually, hydrogen, a hydroxyl group, a halo substituent, an unsubstituted lower alkyl group, a hydroxy (lower) alkyl group, a halo (lower) alkyl group, a phenyl group, a halophenyl group, an alkoxyphenyl group, a hydroxyalkoxy group, a cyano group or an amide group, with at least one of X and X.sup.1 being other than hydrogen; andR.sub.1 and R.sub.2, individually or together, represent an organic substituent that does not interfere with the production of light when the dioxetane compound is enzymatically cleaved and that satisfies the valence of the dioxetane compound's 4-carbon atom, with the provisos that if R.sub.1 and R.sub.2 represent individual substituents the R.sub.2 substituent is aromatic, heteroaromatic, or an unsaturated substituent in conjugation with an aromatic ring, and that at least one of R.sub.1 and R.sub.2 is, or R.sub.1 and R.sub.2 taken together are, an enzymatically cleavable labile group-substituted fluorescent chromophore group that produces a luminescent substance when the enzymatically cleavable labile substituent thereof is removed by an enzyme.The corresponding dioxetanes which, instead of being substituted at the 5' or 7', or at the 5' and 7' positions, instead contain a 4' methylene group, are also disclosed, as are intermediates for all these 3-substituted adamant-2'-ylidenedioxetanes, and their use as reporter molecules in assays.
摘要翻译：公开了能够在分解时产生光能的酶促切割的化学发光的1,2-二氧杂环丁烷化合物，在其中有可能将可酶切割的不稳定取代基故意断裂的键之间在室温下基本稳定。这些化合物可以由下式表示：其中：X和X1各自独立地表示氢，羟基，卤素取代基，未取代的低级烷基，羟基（低级）烷基，卤代（低级））烷基，苯基，卤代苯基，烷氧基苯基，羟基烷氧基，氰基或酰胺基，其中X和X 1中的至少一个不是氢; 并且R 1和R 2各自或一起表示当二氧杂环丁烷化合物被酶切并且满足二恶丁环化合物的4-碳原子的化合价时不干扰光的产生的有机取代基，条件是如果R1和 R2代表单个取代基，R2取代基是芳族的，杂芳族的，或与芳环共轭的不饱和取代基，并且R1和R2中的至少一个是，或者R1和R2一起是可酶切的不稳定基取代的荧光当其酶促切割的不稳定取代基被酶除去时产生发光物质的发色团。也公开了相对于在5'或7'或5'和7'位置取代含有4'亚甲基的二氧杂环丁烷，以及所有这些3-取代的金刚烷基 - 2'-亚基二氧杂环丁烷，以及它们在测定中作为报道分子的用途。

6. 发明授权

US07399645B2 Constrained cis-diol-borate bioconjugation system 失效
标题翻译：约束顺式 - 二醇 - 硼酸盐生物共轭体系
公开(公告)号：US07399645B2
公开(公告)日：2008-07-15
申请号：US11126837
申请日：2005-05-11
申请人： Ricky F. Baggio , Alison L. Sparks , Rouh-Rong Juo , Jaime E. Arenas
发明人： Ricky F. Baggio , Alison L. Sparks , Rouh-Rong Juo , Jaime E. Arenas
IPC分类号： G01N33/547 , G01N33/543 , G01N33/532 , C07K1/10 , C07K1/13 , C07F5/02
CPC分类号： B82Y30/00 , A61K47/54 , C07H21/04 , G01N33/54353 , Y10S530/812
摘要： The invention pertains to bioconjugation systems comprising sterically constrained cis-diols and borates.
摘要翻译：本发明涉及包含空间受限的顺式二醇和硼酸盐的生物共轭体系。

7. 发明申请

US20070287825A1 Constrained cis-diol-borate bioconjugation system 审中-公开
标题翻译：约束顺式 - 二醇 - 硼酸盐生物共轭体系
公开(公告)号：US20070287825A1
公开(公告)日：2007-12-13
申请号：US11800341
申请日：2007-05-04
申请人： Ricky Baggio , Alison Sparks , Rouh-Rong Juo , Jaime Arenas
发明人： Ricky Baggio , Alison Sparks , Rouh-Rong Juo , Jaime Arenas
IPC分类号： C07C321/12 , C07C33/18 , C07C53/00 , C07D209/02 , C07D317/70 , C07D487/00 , C07H21/00 , C07K16/00 , C07K2/00 , C08B37/00
CPC分类号： B82Y30/00 , A61K47/54 , C07H21/04 , G01N33/54353 , Y10S530/812
摘要： The invention pertains to bioconjugation systems comprising sterically constrained cis-diols and borates.
摘要翻译：本发明涉及包含空间受限的顺式二醇和硼酸盐的生物共轭体系。

8. 发明申请

US20050277143A1 Constrained cis-diol-borate bioconjugation system 失效
标题翻译：约束顺式 - 二醇 - 硼酸盐生物共轭体系
公开(公告)号：US20050277143A1
公开(公告)日：2005-12-15
申请号：US11126837
申请日：2005-05-11
申请人： Ricky Baggio , Alison Sparks , Rouh-Rong Juo , Jaime Arenas
发明人： Ricky Baggio , Alison Sparks , Rouh-Rong Juo , Jaime Arenas
IPC分类号： A61K47/48 , C07H21/04 , C07K14/47 , C12Q1/68 , G01N33/53 , G01N33/543
CPC分类号： B82Y30/00 , A61K47/54 , C07H21/04 , G01N33/54353 , Y10S530/812
摘要： The invention pertains to bioconjugation systems comprising sterically constrained cis-diols and borates.
摘要翻译：本发明涉及包含空间受限的顺式二醇和硼酸盐的生物共轭体系。

9. 发明授权

US09067910B2 Chemiluminescent compositions, methods, assays and kits for oxidative enzymes 有权
标题翻译：用于氧化酶的化学发光组合物，方法，测定和试剂盒
公开(公告)号：US09067910B2
公开(公告)日：2015-06-30
申请号：US13254154
申请日：2010-03-01
申请人： Rouh-Rong Juo , Brooks Edwards , Melissa Gee , Zhixian Wang , Kathleen Skaare
发明人： Rouh-Rong Juo , Brooks Edwards , Melissa Gee , Zhixian Wang , Kathleen Skaare
IPC分类号： C07D305/14 , C07D493/00 , C07D321/00 , C07D493/10 , C07D493/04 , C07D407/04 , C12Q1/26
CPC分类号： C12Q1/26 , C07D305/14 , C07D321/00 , C07D323/00 , C07D407/04 , C07D409/04 , C07D493/04 , C07D493/10 , G01N2333/90245 , G01N2333/90251 , G01N2333/90258
摘要： Chemiluminescent compositions, methods, assays and kits for oxidative enzymes are described. Further disclosed are dioxetane compounds of the form: where R can independently be any branched alkyl or cycloalkyl group which provides stabilization for the dioxetane or where both R groups together form a cycloalkyl or polycycloalkyl moiety spiro bound to the dioxetane ring, wherein each R group or the spiro bound moiety can be unsubstituted or substituted with one or more electron-withdrawing groups or electron donating groups, or groups providing preferential oxidative isozyme substrate recognition, and wherein R1 is an aryl group, or an alkyl group of 1-20 carbon atoms, which can be optionally substituted with 1 or more halogen atoms, and wherein T is an aryl or heteroaryl ring capable of emitting light upon enzyme activated decomposition of the dioxetane I. Kits, methods and assays are also disclosed that comprise the dioxetane compounds.
摘要翻译：描述了用于氧化酶的化学发光组合物，方法，测定和试剂盒。进一步公开的是以下形式的二氧杂环丁烷化合物：其中R可以独立地为任何为二氧杂环丁烷提供稳定化的支链烷基或环烷基，或者其中两个R基团一起形成与二氧杂环丁烷环结合的环烷基或多环烷基部分，其中每个R基团或螺结合部分可以是未取代的或被一个或多个吸电子基团或给电子基团或提供优先氧化同功酶底物识别的基团取代，并且其中R1是芳基或1-20个碳原子的烷基，其可以任选地被1个或多个卤素原子取代，并且其中T是在二氧杂环丁烷I的酶活化分解时能够发光的芳基或杂芳基环。还公开了包含二恶丁环化合物的方法和测定法。

10. 发明申请

US20060216768A1 Dioxetane-nanoparticle assemblies for energy transfer detection systems, methods of making the assemblies, and methods of using the assemblies in bioassays 审中-公开
标题翻译：用于能量转移检测系统的二氧杂环丁烷 - 纳米颗粒组件，制造组件的方法和在生物测定中使用组件的方法
公开(公告)号：US20060216768A1
公开(公告)日：2006-09-28
申请号：US11221895
申请日：2005-09-09
申请人： Alison Sparks , Zhixian Wang , Brooks Edwards , Rouh-Rong Juo
发明人： Alison Sparks , Zhixian Wang , Brooks Edwards , Rouh-Rong Juo
IPC分类号： G01N33/533
CPC分类号： G01N33/588 , B82Y15/00 , B82Y30/00 , G01N33/581
摘要： Assemblies comprising nanoparticles and chemiluminescent substrates such as dioxetanes are provided. The assemblies can be used in assays to detect the presence and/or amount of a single analyte or multiple analytes in a sample. Methods of making the assemblies are also described.
摘要翻译：提供了包含纳米颗粒和化学发光底物如二氧杂环丁烷的组件。组合物可用于测定以检测样品中单一分析物或多个分析物的存在和/或量。还描述了制造组件的方法。

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