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1. 发明授权

US06319696B1 Process for producing L-amino acids 失效
标题翻译：生产L-氨基酸的方法
公开(公告)号：US06319696B1
公开(公告)日：2001-11-20
申请号：US09011762
申请日：1998-04-17
申请人： Hiroko Kishino , Masako Izui , Yukiko Ono , Hisao Ito , Osamu Kurahashi
发明人： Hiroko Kishino , Masako Izui , Yukiko Ono , Hisao Ito , Osamu Kurahashi
IPC分类号： C12P1308
CPC分类号： C12P13/04
摘要： A microorganism, which has an ability to produce L-amino acid, especially L-phenylalanine, L-tryptophane, L-tyrosine, L-threonine, or L-isoleucine, in which a phosphoenolpyruvate-producing ability is enhanced, is cultivated in a medium so that the L-amino acid is produced and accumulated in the medium to collect the L-amino acid.
摘要翻译：具有生产L-氨基酸，特别是L-苯丙氨酸，L-色氨酸，L-酪氨酸，L-苏氨酸或L-异亮氨酸的能力的微生物，其中增加了产生磷酸烯醇丙酮酸的能力，培养基中，使L-氨基酸产生并积累在培养基中以收集L-氨基酸。

2. 发明授权

US07297521B2 Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 有权
标题翻译：棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法
公开(公告)号：US07297521B2
公开(公告)日：2007-11-20
申请号：US11011701
申请日：2004-12-15
申请人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
发明人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
IPC分类号： C12P13/10
CPC分类号： C12N9/93 , C12P13/10
摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.
摘要翻译：编码以下（a）或（b）中定义的多肽的DNA片段和以下（c）或（d）中定义的多肽：（a）至少具有氨基酸的氨基酸序列的多肽序列表所示的SEQ ID NO：2中的50〜393的酸值，（b）至少具有序列表所示的序列号2所示的氨基酸序列号为50〜393的氨基酸序列的多肽，包括取代，一个或几个氨基酸的缺失，插入，添加或倒位，并且可以构成具有氨基甲酰磷酸合成酶活性的蛋白质，其具有氨基酸序列SEQ ID NO：3的氨基甲酰磷酸合成酶的大亚基，（c ）具有序列表所示的SEQ ID NO：3的氨基酸序列的多肽，（d）具有序列表所示的SEQ ID NO：3的氨基酸序列的多肽，包括取代，缺失，插入，添加，或反转一个或几个氨基酸，并可构成具有氨基酸 - 磷酸合成酶活性的蛋白质，其具有SEQ ID NO：2中氨基酸序列号为50〜393的氨基酸序列的氨基甲酰磷酸合成酶的小亚基。

3. 发明授权

US06881566B2 Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 有权
标题翻译：棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法
公开(公告)号：US06881566B2
公开(公告)日：2005-04-19
申请号：US09836470
申请日：2001-04-18
申请人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
发明人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
IPC分类号： C12N9/00 , C12P13/10 , C12N1/20 , C07H21/04
CPC分类号： C12P13/10 , C12N9/93
摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID No: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.
摘要翻译：编码以下（a）或（b）中定义的多肽的DNA片段和以下（c）或（d）中定义的多肽：（a）至少具有氨基酸的氨基酸序列的多肽序列表所示的SEQ ID NO：2中的酸值为50〜393，（b）至少具有序列表所示的SEQ ID No：2的氨基酸序列号为50〜393的氨基酸序列的多肽，包括取代，一个或几个氨基酸的缺失，插入，添加或倒位，并且可以构成具有氨基甲酰基 - 磷酸合成酶活性的蛋白质，其具有氨基酸 - 磷酸合成酶的大亚基，其氨基酸序列至少包含氨基酸数55至 SEQ ID NO：3的1113，（c）具有至少包含序列表所示的SEQ ID NO：3的氨基酸编号55至1113的氨基酸序列的多肽，（d）具有氨基酸的多肽序列至少包含氨基酸编号55至11 包括序列表中所示的SEQ ID NO：3的13个氨基酸残基，其包含一个或多个氨基酸的取代，缺失，插入，添加或倒位，并且可以构成具有氨甲酰磷酸合成酶活性的氨基甲酰磷酸合成酶的小亚基具有SEQ ID NO：2中氨基酸序列号50至393的氨基酸序列。

4. 发明授权

US07608437B2 Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains 有权
标题翻译：构建产氨酸的细菌菌株的方法，以及用构建的产生氨基酸的菌株通过发酵制备氨基酸的方法
公开(公告)号：US07608437B2
公开(公告)日：2009-10-27
申请号：US11218433
申请日：2005-09-06
申请人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12P13/14 , C12Q1/48 , C12Q1/32 , C12Q1/68 , C12N9/02 , C12N9/10 , C12N1/21 , C12P21/00 , C12N15/00 , C07K14/00 , C07H21/00
CPC分类号： C12P13/14 , C12N15/67
摘要： A method of producing coryneform bacteria having an improved amino acid or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium so that it is close to a consensus sequence or introducing a change in the promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on the chromosome of a coryneform bacterium by gene recombination so that it is close to a consensus sequence, obtaining mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows one of skill in the art to construct a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield, by the recombination or mutation.
摘要翻译：具有改进的氨基酸或核酸生产力的棒状杆菌细菌的方法包括以下步骤：将氨基酸或核酸 - 生物合成基因的启动子序列中的突变引入棒状细菌的染色体上，使其接近共有序列或通过基因重组在棒状细菌的染色体上引入氨基酸或核酸 - 生物合成基因的启动子序列的变化，使得其接近共有序列，获得棒状杆菌型氨基酸的突变体或产生核酸的微生物，培养突变体并选择能够大量产生所需氨基酸或核酸的突变体。该方法允许本领域技术人员通过重组或突变来构建能够富集或控制预期基因的表达而不使用质粒并以高产率促进氨基酸产生的突变体。

5. 发明授权

US07419810B2 Arginine repressor deficient strain of coryneform bacterium and method for producing L-arginine 有权
标题翻译：棒状杆菌型精氨酸阻遏物缺陷菌株及L-精氨酸生产方法
公开(公告)号：US07419810B2
公开(公告)日：2008-09-02
申请号：US11451347
申请日：2006-06-13
申请人： Mikiko Suga , Yoko Asakura , Yukiko Mori , Hisao Ito , Osamu Kurahashi
发明人： Mikiko Suga , Yoko Asakura , Yukiko Mori , Hisao Ito , Osamu Kurahashi
IPC分类号： C12P13/10 , C12N1/00 , C12N1/20 , C07K1/00 , C07H21/04
CPC分类号： C07K14/34
摘要： L-Arginine is produced by culturing a coryneform bacterium in which an arginine repressor involved in L-arginine biosynthesis is deleted by disrupting a gene coding for the repressor, and which has L-arginine producing ability in a medium to produce and accumulate L-arginine in the medium, and collecting the L-arginine from the medium.
摘要翻译： L-精氨酸是通过培养通过破坏编码阻遏物的基因而缺失参与L-精氨酸生物合成的精氨酸阻遏物的棒状细菌产生的，并且在培养基中具有L-精氨酸生产能力以产生和积累L-精氨酸在培养基中，并从培养基中收集L-精氨酸。

6. 发明授权

US07160705B2 Arginine repressor deficient strain of coryneform bacterium and method for producing L-arginine 有权
标题翻译：棒状杆菌型精氨酸阻遏物缺陷菌株及L-精氨酸生产方法
公开(公告)号：US07160705B2
公开(公告)日：2007-01-09
申请号：US09835381
申请日：2001-04-17
申请人： Mikiko Suga , Yoko Asakura , Yukiko Mori , Hisao Ito , Osamu Kurahashi
发明人： Mikiko Suga , Yoko Asakura , Yukiko Mori , Hisao Ito , Osamu Kurahashi
IPC分类号： C12P13/10 , C12N1/00 , C12N1/20 , C07K1/00 , C07H21/04
CPC分类号： C07K14/34
摘要： L-Arginine is produced by culturing a coryneform bacterium in which an arginine repressor involved in L-arginine biosynthesis is deleted by disrupting a gene coding for the repressor, and which has L-arginine producing ability in a medium to produce and accumulate L-arginine in the medium, and collecting the L-arginine from the medium.
摘要翻译： L-精氨酸是通过培养通过破坏编码阻遏物的基因而缺失参与L-精氨酸生物合成的精氨酸阻遏物的棒状细菌产生的，并且在培养基中具有L-精氨酸生产能力以产生和积累L-精氨酸在培养基中，并从培养基中收集L-精氨酸。

7. 发明申请

US20050095680A1 Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 有权
公开(公告)号：US20050095680A1
公开(公告)日：2005-05-05
申请号：US11011701
申请日：2004-12-15
申请人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
发明人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
IPC分类号： C12N1/21 , C12N9/00 , C12P13/10 , C07H21/04 , C12N9/12
CPC分类号： C12N9/93 , C12P13/10
摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.

8. 发明申请

US20070031946A1 Arginine repressor deficient strain of coryneform bacterium and method for producing L-arginine 有权
公开(公告)号：US20070031946A1
公开(公告)日：2007-02-08
申请号：US11451347
申请日：2006-06-13
申请人： Mikiko Suga , Yoko Asakura , Yukiko Mori , Hisao Ito , Osamu Kurahashi
发明人： Mikiko Suga , Yoko Asakura , Yukiko Mori , Hisao Ito , Osamu Kurahashi
IPC分类号： C12P13/10 , C12N1/21
CPC分类号： C07K14/34
摘要： L-Arginine is produced by culturing a coryneform bacterium in which an arginine repressor involved in L-arginine biosynthesis is deleted by disrupting a gene coding for the repressor, and which has L-arginine producing ability in a medium to produce and accumulate L-arginine in the medium, and collecting the L-arginine from the medium.

9. 发明申请

US20060003424A1 Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains 有权
标题翻译：构建产氨酸的细菌菌株的方法，以及用构建的产生氨基酸的菌株通过发酵制备氨基酸的方法
公开(公告)号：US20060003424A1
公开(公告)日：2006-01-05
申请号：US11218433
申请日：2005-09-06
申请人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12P13/22 , C12P13/14 , C12P13/08 , C12N1/21
CPC分类号： C12P13/14 , C12N15/67
摘要： A method of producing coryneform bacteria having an improved amino acid- or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium to make it close to a consensus sequence or introducing a change in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium by gene recombination to make it close to a consensus sequence, to obtain mutants of the coryneform amino acid d- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method can construct a mutant capable of suitably enriching or controling the expression of an intended gene without using a plasmid and also capable of producing amino acids in a high yield, by the recombination or mutation.
摘要翻译：具有改善的氨基酸或核酸生产力的棒状细菌的方法包括以下步骤：在棒状细菌的染色体上的氨基酸或核酸 - 生物合成基因的启动子序列中引入突变，使其接近共有序列或通过基因重组在棒状细菌的染色体上引入氨基酸或核酸 - 生物合成基因的启动子序列的变化，使其接近共有序列，获得棒状杆菌氨基酸d- 或产生核酸的微生物，培养突变体并选择能够大量产生所需氨基酸或核酸的突变体。该方法可以构建能够适当地富集或控制预期基因的表达而不使用质粒并且还能够通过重组或突变以高产率产生氨基酸的突变体。

10. 发明授权

US06962805B2 Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains 有权
标题翻译：构建产氨酸的细菌菌株的方法，以及用构建的产生氨基酸的菌株通过发酵制备氨基酸的方法
公开(公告)号：US06962805B2
公开(公告)日：2005-11-08
申请号：US09577005
申请日：2000-05-25
申请人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12N15/37 , C12N1/21 , C12N15/67 , C12P13/04 , C12P13/14 , C07H21/04 , C12N1/20 , C12N15/74 , C12Q1/32
CPC分类号： C12P13/14 , C12N15/67
摘要： A method of producing coryneform bacteria having improved amino acid or nucleic acid productivity comprising the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium to make it close to a consensus sequence, or introducing a change in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium by gene recombination to make it close to a consensus sequence, to obtain mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and selecting a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows the construction of a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield by recombination or mutation.
摘要翻译：制备具有改善的氨基酸或核酸生产力的棒状细菌的方法包括以下步骤：在棒状细菌染色体上的氨基酸或核酸 - 生物合成基因的启动子序列中引入突变，使其接近共有序列或通过基因重组在棒状细菌的染色体上引入氨基酸或核酸 - 生物合成基因的启动子序列的变化使其接近共有序列，以获得棒状细胞氨基酸或核酸的突变体培养微生物，培养突变体并选择能够大量产生所需氨基酸或核酸的突变体。该方法允许构建能够富集或控制预期基因的表达而不使用质粒的突变体，并通过重组或突变促进高产量的氨基酸的产生。

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