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1. 发明申请

US20110033902A1 L-CYSTEINE-PRODUCING BACTERIUM AND A METHOD FOR PRODUCING L-CYSTEINE 有权
标题翻译： L-丝氨酸生产细菌和生产L-儿茶素的方法
公开(公告)号：US20110033902A1
公开(公告)日：2011-02-10
申请号：US12858590
申请日：2010-08-18
申请人： Gen Nonaka , Hiroshi Takagi , Iwao Ohtsu
发明人： Gen Nonaka , Hiroshi Takagi , Iwao Ohtsu
IPC分类号： C12P13/12 , C12N1/21 , C12P13/06
CPC分类号： C12N1/20 , C07K14/245 , C12P13/12
摘要： L-Cysteine, L-cystine, a derivative or precursor thereof, or a mixture thereof is produced by culturing a bacterium belonging to the family Enterobacteriaceae, which has L-cysteine-producing ability and has been modified so that the activity of a protein encoded by a tolC gene, for example, a protein defined in the following (a) or (b), is increased in a medium, and by collecting L-cysteine, L-cystine, a derivative or precursor thereof, or a mixture thereof from the medium: (a) a protein comprising the amino acid sequence of SEQ ID NO: 2, (b) a protein comprising the amino acid sequence of SEQ ID NO: 2, but wherein one or several amino acid residues are substituted, deleted, inserted or added, increase of which activity in the bacterium improves the ability of the bacterium to produce L-cysteine.
摘要翻译： L-半胱氨酸，L-胱氨酸，其衍生物或其前体或其混合物通过培养属于具有L-半胱氨酸生产能力并已经被修饰的家族肠杆菌科的细菌产生，使得蛋白质的活性被编码通过tolC基因，例如在以下（a）或（b）中定义的蛋白质在培养基中增加，并且通过将L-半胱氨酸，L-胱氨酸，其衍生物或其前体或其混合物从培养基：（a）包含SEQ ID NO：2的氨基酸序列的蛋白质，（b）包含SEQ ID NO：2的氨基酸序列的蛋白质，但其中一个或几个氨基酸残基被取代，缺失，插入或添加，细菌中哪种活性的增加提高了细菌产生L-半胱氨酸的能力。

2. 发明授权

US08293506B2 L-cysteine-producing bacterium and a method for producing L-cysteine 有权
标题翻译： L-半胱氨酸生产细菌和L-半胱氨酸的生产方法
公开(公告)号：US08293506B2
公开(公告)日：2012-10-23
申请号：US12711299
申请日：2010-02-24
申请人： Gen Nonaka , Hiroshi Takagi , Iwao Ohtsu , Natthawut Wiriyathanawudhiwong , Takeshi Nakatani
发明人： Gen Nonaka , Hiroshi Takagi , Iwao Ohtsu , Natthawut Wiriyathanawudhiwong , Takeshi Nakatani
IPC分类号： C12P13/12 , C12N1/20
CPC分类号： C12P13/12 , C12N9/0051
摘要： The present invention provides a bacterium belonging to the family Enterobacteriaceae, which is able to produce L-cysteine and has been modified to increase the activity of the protein encoded by the dsbA gene. This bacterium is cultured in a medium, and L-cysteine, L-cystine, a derivative or precursor thereof or a mixture thereof can be collected from the medium.
摘要翻译：本发明提供属于肠杆菌科的细菌，其能够产生L-半胱氨酸，并且已被修饰以提高由dsbA基因编码的蛋白质的活性。将该细菌培养于培养基中，从培养基中收集L-半胱氨酸，L-胱氨酸，其衍生物或其前体或其混合物。

3. 发明授权

US08278075B2 L-cysteine-producing bacterium and a method for producing L-cysteine 有权
标题翻译： L-半胱氨酸生产细菌和L-半胱氨酸的生产方法
公开(公告)号：US08278075B2
公开(公告)日：2012-10-02
申请号：US12858590
申请日：2010-08-18
申请人： Gen Nonaka , Hiroshi Takagi , Iwao Ohtsu
发明人： Gen Nonaka , Hiroshi Takagi , Iwao Ohtsu
IPC分类号： C12P13/12 , C12P13/06 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N1/20 , C07K14/245 , C12P13/12
摘要： L-Cysteine, L-cystine, a derivative or precursor thereof, or a mixture thereof is produced by culturing a bacterium belonging to the family Enterobacteriaceae, which has L-cysteine-producing ability and has been modified so that the activity of a protein encoded by a tolC gene, for example, a protein defined in the following (a) or (b), is increased in a medium, and by collecting L-cysteine, L-cystine, a derivative or precursor thereof, or a mixture thereof from the medium: (a) a protein comprising the amino acid sequence of SEQ ID NO: 2, (b) a protein comprising the amino acid sequence of SEQ ID NO: 2, but wherein one or several amino acid residues are substituted, deleted, inserted or added, increase of which activity in the bacterium improves the ability of the bacterium to produce L-cysteine.
摘要翻译： L-半胱氨酸，L-胱氨酸，其衍生物或其前体或其混合物通过培养属于具有L-半胱氨酸生产能力并已经被修饰的家族肠杆菌科的细菌产生，使得蛋白质的活性被编码通过tolC基因，例如在以下（a）或（b）中定义的蛋白质在培养基中增加，并且通过将L-半胱氨酸，L-胱氨酸，其衍生物或其前体或其混合物从培养基：（a）包含SEQ ID NO：2的氨基酸序列的蛋白质，（b）包含SEQ ID NO：2的氨基酸序列的蛋白质，但其中一个或几个氨基酸残基被取代，缺失，插入或添加，细菌中哪种活性的增加提高了细菌产生L-半胱氨酸的能力。

4. 发明申请

US20100216196A1 L-CYSTEINE-PRODUCING BACTERIUM AND A METHOD FOR PRODUCING L-CYSTEINE 有权
标题翻译： L-丝氨酸生产细菌和生产L-儿茶素的方法
公开(公告)号：US20100216196A1
公开(公告)日：2010-08-26
申请号：US12711299
申请日：2010-02-24
申请人： Gen Nonaka , Hiroshi Takagi , Iwao Ohtsu , Natthawut Wiriyathanawudhiwong , Takeshi Nakatani
发明人： Gen Nonaka , Hiroshi Takagi , Iwao Ohtsu , Natthawut Wiriyathanawudhiwong , Takeshi Nakatani
IPC分类号： C12P13/12 , C12N1/21
CPC分类号： C12P13/12 , C12N9/0051
摘要： The present invention provides a bacterium belonging to the family Enterobacteriaceae, which is able to produce L-cysteine and has been modified to increase the activity of the protein encoded by the dsbA gene. This bacterium is cultured in a medium, and L-cysteine, L-cystine, a derivative or precursor thereof or a mixture thereof can be collected from the medium.
摘要翻译：本发明提供属于肠杆菌科的细菌，其能够产生L-半胱氨酸，并且已被修饰以提高由dsbA基因编码的蛋白质的活性。将该细菌培养于培养基中，从培养基中收集L-半胱氨酸，L-胱氨酸，其衍生物或其前体或其混合物。

5. 发明授权

US08460903B2 Method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family 有权
标题翻译：使用肠杆菌科的细菌生产L-氨基酸的方法
公开(公告)号：US08460903B2
公开(公告)日：2013-06-11
申请号：US13004188
申请日：2011-01-11
申请人： Ekaterina Alekseevna Savrasova , Natalia Viktorovna Stoynova , Gen Nonaka , Shunsuke Yamazaki , Kazuhiro Takumi
发明人： Ekaterina Alekseevna Savrasova , Natalia Viktorovna Stoynova , Gen Nonaka , Shunsuke Yamazaki , Kazuhiro Takumi
IPC分类号： C12P13/04 , C12P13/12 , C12P13/22
CPC分类号： C12P13/04 , C07K14/24 , C07K14/245 , C12N15/74 , C12P13/06 , C12P13/08 , C12P13/12
摘要： A method for producing an L-amino acid is described using a bacterium of the Enterobacteriaceae family, wherein the bacterium contains a protein which is able to confer resistance to growth inhibition by L-cysteine.
摘要翻译：使用肠杆菌科的细菌描述L-氨基酸的制备方法，其中该细菌含有能够赋予L-半胱氨酸对生长抑制的抗性的蛋白质。

6. 发明申请

US20120252076A1 METHOD FOR PRODUCING L-CYSTEINE 有权
标题翻译：生产L-儿茶素的方法
公开(公告)号：US20120252076A1
公开(公告)日：2012-10-04
申请号：US13431104
申请日：2012-03-27
申请人： Shunsuke Yamazaki , Gen Nonaka , Kazuhiro Takumi
发明人： Shunsuke Yamazaki , Gen Nonaka , Kazuhiro Takumi
IPC分类号： C12P13/12
CPC分类号： C12P13/12 , C12N9/001 , C12N9/0051 , C12N9/1007 , C12N9/88 , C12Y103/01076 , C12Y108/99001 , C12Y201/01107 , C12Y402/99 , C12Y499/01004
摘要： A method for producing L-cysteine and the like is provided by developing a novel technique for improving bacterial L-cysteine-producing ability. The method includes the steps of culturing a bacterium belonging to the genus Escherichia which has L-cysteine-producing ability, and in which expression of a gene involved in sulfite reduction is enhanced, in a medium containing thiosulfate, and collecting L-cysteine, a related substance thereof, or a mixture thereof which accumulate in the medium.
摘要翻译：通过开发用于改善细菌L-半胱氨酸生产能力的新技术，提供L-半胱氨酸的生产方法。该方法包括以下步骤：在含有硫代硫酸盐的培养基中培养属于具有L-半胱氨酸生产能力的埃希氏菌属细菌，其中涉及亚硫酸盐还原的基因的表达增强，并收集L-半胱氨酸的步骤，其相关物质或其在培养基中积累的混合物。

7. 发明授权

US08206954B2 L-amino acid-producing microorganism and a method for producing an L-amino acid 有权
标题翻译： L-氨基酸生产微生物和L-氨基酸的生产方法
公开(公告)号：US08206954B2
公开(公告)日：2012-06-26
申请号：US13037557
申请日：2011-03-01
申请人： Rie Takikawa , Yoshihiko Hara , Gen Nonaka , Kazuhiro Takumi
发明人： Rie Takikawa , Yoshihiko Hara , Gen Nonaka , Kazuhiro Takumi
IPC分类号： C12P13/24
CPC分类号： C12P13/04 , C12N9/0006 , C12P13/06 , C12P13/08 , C12P13/12 , C12P13/14 , C12P13/22 , C12Y101/05002
摘要： An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae, which has an L-amino acid-producing ability and inherently has a native activity of a glucose dehydrogenase that uses pyrroloquinoline quinone as a coenzyme, but has been modified so that the activity of the glucose dehydrogenase is reduced, in a medium, and collecting the L-amino acid from the medium.
摘要翻译：通过培养属于肠杆菌科的细菌产生L-氨基酸，其具有L-氨基酸生产能力，并且固有地具有使用吡咯并喹啉醌作为辅酶的葡萄糖脱氢酶的天然活性，但已被修饰在培养基中葡萄糖脱氢酶的活性降低，并从培养基中收集L-氨基酸。

8. 发明授权

US08008048B2 L-cysteine-producing bacterium and a method for producing L-cysteine 有权
标题翻译： L-半胱氨酸生产细菌和L-半胱氨酸的生产方法
公开(公告)号：US08008048B2
公开(公告)日：2011-08-30
申请号：US12397666
申请日：2009-03-04
申请人： Gen Nonaka , Kazuhiro Takumi
发明人： Gen Nonaka , Kazuhiro Takumi
IPC分类号： C12P13/12 , C07K14/00 , C07H21/00 , C12N1/21 , C12N15/00 , C12P21/00
CPC分类号： C12P13/12 , C07K14/245 , C12N1/20
摘要： The present invention provides a bacterium belonging to the family Enterobacteriaceae which has L-cysteine-producing ability and has been modified to decrease the activity of the protein encoded by the yhaM gene. This bacterium is cultured in a medium, and L-cysteine, L-cystine, their derivatives, or a mixture thereof is collected from the medium.
摘要翻译：本发明提供一种属于肠杆菌科的细菌，其具有L-半胱氨酸生产能力，并被修饰以降低由yhaM基因编码的蛋白质的活性。将该细菌在培养基中培养，从培养基中收集L-半胱氨酸，L-胱氨酸，其衍生物或其混合物。

9. 发明申请

US20050239176A1 Genes for heat resistant enzymes of amino acid biosynthetic pathway derived from thermophilic coryneform bacteria 有权
标题翻译：来自嗜热棒状杆菌的氨基酸生物合成途径耐热酶基因
公开(公告)号：US20050239176A1
公开(公告)日：2005-10-27
申请号：US11073560
申请日：2005-03-08
申请人： Seiko Hirano , Eiichiro Kimura , Tsuyoshi Osumi , Kazuhiko Matsui , Yoshio Kawahara , Gen Nonaka , Yumi Matsuzaki , Naoki Akiyoshi , Kanae Nakamura , Osamu Kurahashi , Tsuyoshi Nakamatsu , Shinichi Sugimoto
发明人： Seiko Hirano , Eiichiro Kimura , Tsuyoshi Osumi , Kazuhiko Matsui , Yoshio Kawahara , Gen Nonaka , Yumi Matsuzaki , Naoki Akiyoshi , Kanae Nakamura , Osamu Kurahashi , Tsuyoshi Nakamatsu , Shinichi Sugimoto
IPC分类号： C07K14/34 , C12N9/02 , C12N9/04 , C12N9/12 , C12N9/26 , C12N9/88 , C12N15/31 , C12N15/53 , C12N15/54 , C12N15/56 , C12N15/60 , C12P13/04 , C07H21/04 , C12N9/10 , C12N15/74 , C12P13/08
CPC分类号： C12Y108/01004 , C07K14/34 , C12N9/0006 , C12N9/0008 , C12N9/0051 , C12N9/1205 , C12N9/2408 , C12N9/88 , C12Y101/01041 , C12Y102/01051 , C12Y102/04002 , C12Y104/01004 , C12Y203/03001 , C12Y207/01011 , C12Y302/01026 , C12Y401/01031 , C12Y401/03001 , C12Y402/01003 , C12Y604/01001 , C12Y604/01002
摘要： A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.
摘要翻译：基于对应于编码源自热棒状棒杆菌的L-氨基酸生物合成途径的酶的基因的已知基因序列的各种微生物，观察到在氨基酸水平上保持的区域的多个引物组，优选为与谷氨酸棒杆菌相比在更高的温度下起作用的酶。通过使用热源性棒状杆菌的引物和染色体DNA作为模板进行PCR。使用已经获得扩增片段的引物作为引物用于筛选，从嗜热氨基酸棒杆菌的染色体DNA的质粒文库中选择含有靶DNA片段的克隆。

10. 发明申请

US20050176127A1 Genes for heat resistant enzymes of amino acid biosynthetic pathway derived from thermophilic coryneform bacteria 有权
公开(公告)号：US20050176127A1
公开(公告)日：2005-08-11
申请号：US11073550
申请日：2005-03-08
申请人： Seiko Hirano , Eiichiro Kimura , Tsuyoshi Osumi , Kazuhiko Matsui , Yoshio Kawahara , Gen Nonaka , Yumi Matsuzaki , Naoki Akiyoshi , Kanae Nakamura , Osamu Kurahashi , Tsuyoshi Nakamatsu , Shinichi Sugimoto
发明人： Seiko Hirano , Eiichiro Kimura , Tsuyoshi Osumi , Kazuhiko Matsui , Yoshio Kawahara , Gen Nonaka , Yumi Matsuzaki , Naoki Akiyoshi , Kanae Nakamura , Osamu Kurahashi , Tsuyoshi Nakamatsu , Shinichi Sugimoto
IPC分类号： C07K14/34 , C12N9/02 , C12N9/04 , C12N9/12 , C12N9/26 , C12N9/88 , C12N15/31 , C12N15/53 , C12N15/54 , C12N15/56 , C12N15/60 , C12N1/21 , C12N15/74 , C12P13/08
CPC分类号： C12Y108/01004 , C07K14/34 , C12N9/0006 , C12N9/0008 , C12N9/0051 , C12N9/1205 , C12N9/2408 , C12N9/88 , C12Y101/01041 , C12Y102/01051 , C12Y102/04002 , C12Y104/01004 , C12Y203/03001 , C12Y207/01011 , C12Y302/01026 , C12Y401/01031 , C12Y401/03001 , C12Y402/01003 , C12Y604/01001 , C12Y604/01002
摘要： A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.

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