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1. 发明申请

WO2005083053A3 METHOD FOR PRODUCING UNSATURATED OMEGA-3 FATTY ACIDS IN TRANSGENIC ORGANISMS 审中-公开
标题翻译：用于生产不饱和ω-3脂肪酸的转基因生物
公开(公告)号：WO2005083053A3
公开(公告)日：2005-11-10
申请号：PCT/EP2005001865
申请日：2005-02-23
申请人： BASF PLANT SCIENCE GMBH , CIRPUS PETRA , BAUER JOERG , ZANK THORSTEN , HEINZ ERNST
发明人： CIRPUS PETRA , BAUER JOERG , ZANK THORSTEN , HEINZ ERNST
IPC分类号： C12N9/02 , C12N15/82 , C12P7/64 , C12N5/10 , C12N15/53
CPC分类号： C12N9/0071 , C12N9/0004 , C12N15/8247
摘要： The invention relates to a method for producing unsaturated O3 fatty acids, and to a method for producing triglycerides having an increased unsaturated fatty acid content, especially O3 fatty acids with more than three double bonds. The invention also relates to the production of a transgenic organism, preferably a transgenic plant or a transgenic micro-organism, with an increased content of unsaturated O3 double bonds on the basis of the expression of a O3 desaturase of fungi of the pythiacae family, such as the species phytophtora, for example the species and type phytophtora infestans. The invention further relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing at least one inventive nucleic acid sequence, at least one vector containing the nucleic acid sequence and/or the nucleic acid constructs, and transgenic organisms containing the cited nucleic acid sequences, nucleic acid constructs and/or vectors. Another part of the invention relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use thereof. Furthermore, the invention relates to unsaturated fatty acids and triglycerides having an increased unsaturated fatty acid content, and to the use of the same.
摘要翻译：本发明涉及一种用于生产不饱和ω-3脂肪酸和用于生产具有的不饱和脂肪酸含量增加的甘油三酯的制造方法，具有多于三个双键尤其ω-3脂肪酸。本发明涉及产生转基因生物，优选的转基因植物或转基因微生物，其具有具有由于ω-3去饱和酶的来自家庭Pythiaceae的真菌表达ω-3双键的不饱和的ω-3脂肪酸，油或脂质的含量增加如属疫，例如属和种致病疫霉。本发明还涉及包含本发明的至少一种核酸序列，含有包含上述核酸序列，核酸构建体和/或载体的核酸序列和/或核酸构建体，和转基因生物的至少一种载体中的核酸序列，核酸构建体，载体，和生物体。本发明的另一部分涉及根据本发明和它们的使用由该方法产生的油，脂质和/或脂肪酸。本发明还涉及到的不饱和脂肪酸和甘油三酯具有不饱和脂肪酸的含量增加及其用途。

2. 发明申请

WO2005012316A3 METHOD FOR THE PRODUCTION OF MULTIPLY-UNSATURATED FATTY ACIDS IN TRANSGENIC ORGANISMS 审中-公开
标题翻译：用于生产不饱和脂肪酸的转基因多
公开(公告)号：WO2005012316A3
公开(公告)日：2005-10-06
申请号：PCT/EP2004007957
申请日：2004-07-16
申请人： BASF PLANT SCIENCE GMBH , ZANK THORSTEN , BAUER JOERG , CIRPUS PETRA , ABBADI AMINE , HEINZ ERNST , QIU XIAO , VRINTEN PATRICIA , SPERLING PETRA , DOMERGUE FREDERIC , MEYER ASTRID , KIRSCH JELENA
发明人： ZANK THORSTEN , BAUER JOERG , CIRPUS PETRA , ABBADI AMINE , HEINZ ERNST , QIU XIAO , VRINTEN PATRICIA , SPERLING PETRA , DOMERGUE FREDERIC , MEYER ASTRID , KIRSCH JELENA
IPC分类号： A23D9/00 , C11B1/02 , C12N9/02 , C12N9/10 , C12N15/82 , C12P7/64 , A01K67/027
CPC分类号： C12N15/8247 , A23D9/00 , A23D9/02 , A23L33/115 , A23V2002/00 , C11B1/00 , C12N9/0071 , C12N9/0083 , C12N9/0093 , C12N9/1029 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12Y114/19 , C12Y114/19003 , C12Y203/01199 , Y02P20/52
摘要： The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with Delta-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a Delta-6 desaturase, a Delta-5 desaturase, Delta-4 desaturase and/or Delta-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated omega-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of Delta-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of omega-3 fatty acids, oils or lipids with omega-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with omega-3 desaturases, for example a omega-3 desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example, the genus and species Phytophtora infestans, or a omega-3 desaturase from algae such as the family Prasinophyceae, for example, the genus Ostreococcus and, particularly, the genus and species Ostreococcus tauri or diatomaceae such as the genus Thalassiosira and, particularly, the genus and species Thalassiosira pseudonana. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and/or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.
摘要翻译：本发明涉及一种用于通过核酸的生物体制备的多个unge饱和脂肪酸的方法被引入到生物体中，这对于多肽与Δ-5-延伸酶活性的代码。有利地，这些核酸序列可以在生物体中，任选地与编码所述脂肪酸或脂质的生物合成的多肽的另外的核酸序列表达。特别有利的是编码Δ-4-Desaturaseund /或Δ-6去饱和酶，Δ-5去饱和酶的Δ-6延长酶的活性的核酸序列。有利的是，这些去饱和酶和延伸酶海链藻，裸藻或Ostreococcus。此外，本发明涉及用于生产油和/或三酰基甘油酯与长链多不饱和脂肪酸的升高的内容的处理。此外，在一个优选的实施方案中，本发明涉及用于生产不饱和ω-3脂肪酸和用于生产具有的不饱和脂肪酸含量增加的甘油三酯的方法的过程中，具有超过三个双键尤其ω-3脂肪酸。本发明涉及产生转基因生物，优选的转基因植物或转基因微生物，其具有具有由于延伸酶的表达ω-3双键的不饱和的ω-3脂肪酸，油或脂质的含量增加，并在本发明中连接有利的过程中去饱和酶使用与ω-3去饱和酶，例如，从家庭Pythiaceae的真菌，如属疫ω-3去饱和酶，例如属和种致病疫霉或从藻类ω-3去饱和酶诸如家庭绿枝藻纲例如的属Ostreococcus具体的属和种Ostreococcus桃源或硅藻如海链藻属特别是属和种海链pseudonana。本发明还涉及含有本发明核酸序列的核酸序列，核酸构建体，载体和有机体，将含有包含上述核酸序列，核酸构建体和/或载体的核酸序列和/或核酸构建体，和转基因生物体的载体。本发明的另一部分涉及通过本发明和它们的使用过程中产生的油，脂质和/或脂肪酸。本发明还涉及到的不饱和脂肪酸和甘油三酯具有不饱和脂肪酸的含量增加及其用途。

3. 发明申请

WO2008043849A3 PLANTS WITH INCREASED YIELD 审中-公开
标题翻译：植物增加
公开(公告)号：WO2008043849A3
公开(公告)日：2008-09-04
申请号：PCT/EP2007060912
申请日：2007-10-12
申请人： BASF PLANT SCIENCE GMBH , MAX PLANCK INST FUER MOLEKULAR , ZANK THORSTEN , OSWALD OLIVER , BAUER JOERG , ZRENNER RITA MARIA , KOSLOWSKY SILKE
发明人： ZANK THORSTEN , OSWALD OLIVER , BAUER JOERG , ZRENNER RITA MARIA , KOSLOWSKY SILKE
IPC分类号： C12N15/82 , A01H5/10 , C12N9/12
CPC分类号： C12N15/8247 , C12N9/1235 , C12N15/8261 , Y02A40/146
摘要： This invention relates generally to plant cells and/or plants with increased yield as compared to a corresponding non-transformed wild type plant cell by increasing or generating one or more activities of polypeptides associated with the intermediate phosphoribosylpyrophosphate (PRPP) in plants. In particular, this invention relates to plant cells and/or plants with increased yield as compared to a corresponding non- transformed wild type plant cell by increasing or generating one or more activities of phosphoribosyl pyrophosphate synthases (PRPP synthetase, PRS) The invention also deals with methods of producing and screening for and breeding such plant cells and/or plants.
摘要翻译：与相应的未转化的野生型植物细胞相比，通过增加或产生与植物中的中间磷酸核糖基焦磷酸（PRPP）相关的多肽的一种或多种活性，本发明一般涉及具有增加的产量的植物细胞和/或植物。特别地，本发明涉及通过增加或产生磷酸核糖焦磷酸合酶（PRPP合成酶，PRS）的一种或多种活性而与相应的未转化的野生型植物细胞相比具有增加的产量的植物细胞和/或植物。本发明还涉及使用生产和筛选和繁殖这种植物细胞和/或植物的方法。

4. 发明申请

WO2008043849A2 PLANTS WITH INCREASED YIELD 审中-公开
标题翻译：植物产量增加
公开(公告)号：WO2008043849A2
公开(公告)日：2008-04-17
申请号：PCT/EP2007060912
申请日：2007-10-12
申请人： BASF PLANT SCIENCE GMBH , MAX PLANCK INST FUER MOLEKULAR , ZANK THORSTEN , OSWALD OLIVER , BAUER JOERG , ZRENNER RITA MARIA , KOSLOWSKY SILKE
发明人： ZANK THORSTEN , OSWALD OLIVER , BAUER JOERG , ZRENNER RITA MARIA , KOSLOWSKY SILKE
IPC分类号： C12N15/82
CPC分类号： C12N15/8247 , C12N9/1235 , C12N15/8261 , Y02A40/146
摘要： This invention relates generally to plant cells and/or plants with increased yield as compared to a corresponding non-transformed wild type plant cell by increasing or generating one or more activities of polypeptides associated with the intermediate phosphoribosylpyrophosphate (PRPP) in plants. In particular, this invention relates to plant cells and/or plants with increased yield as compared to a corresponding non- transformed wild type plant cell by increasing or generating one or more activities of phosphoribosyl pyrophosphate synthases (PRPP synthetase, PRS) The invention also deals with methods of producing and screening for and breeding such plant cells and/or plants.
摘要翻译：本发明通常涉及通过增加或产生与植物中的中间体磷酸核糖焦磷酸（PRPP）相关的多肽的一种或多种活性而与相应的未转化的野生型植物细胞相比具有增加的产量的植物细胞和/或植物。具体而言，本发明涉及通过增加或产生磷酸核糖焦磷酸合酶（PRPP合成酶，PRS）的一种或多种活性而与相应的未转化的野生型植物细胞相比具有增加的产量的植物细胞和/或植物。本发明还涉及以及生产和筛选和培育这种植物细胞和/或植物的方法。

5. 发明申请

WO2010023202A3 NUCLEIC ACIDS ENCODING DESATURASES AND MODIFIED PLANT OIL 审中-公开
标题翻译：核酸编码和改性植物油
公开(公告)号：WO2010023202A3
公开(公告)日：2010-06-24
申请号：PCT/EP2009060923
申请日：2009-08-25
申请人： BASF PLANT SCIENCE GMBH , BIORIGINAL FOOD & SCIENCE CORP , BAUER JOERG , SENGER TORALF , ZANK THORSTEN , QIU XIAO , WU GUOHAI
发明人： BAUER JOERG , SENGER TORALF , ZANK THORSTEN , QIU XIAO , WU GUOHAI
IPC分类号： C12N15/82 , C12N9/00
CPC分类号： C12N15/8247 , C12N5/14 , C12N9/00 , C12N9/0004
摘要： The present invention relates to nucleic acids derived from Drechslera tritici-repentis, Cylindorcarpon herteronema, Diploida natalensis, Stagonospora nodorum, Microdochium nivalae and Periplaneta americana. The invention also relates to the individual coding sequences and to proteins encoded by these sequences in combination with other sequences as well as to a process for converting oleic acid to linoleic acid to Iinoleic acid and the production of arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid in a plant.
摘要翻译：本发明涉及来自Drechslera tritici-repentis，Cylindorcarpon herteronema，Diploida natalensis，Stagonospora nodorum，Microdochium nivalae和Periplaneta americana的核酸。本发明还涉及个体编码序列和由这些序列与其它序列组合编码的蛋白质以及将油酸转化为亚油酸至亚油酸的方法，以及花生四烯酸，二十碳五烯酸和/或二十二碳六烯酸的生产酸在植物中。

6. 发明申请

WO2004076617A3 METHOD FOR THE PRODUCTION OF POLYUNSATURATED FATTY ACIDS 审中-公开
标题翻译：生产多种不饱和脂肪酸的方法
公开(公告)号：WO2004076617A3
公开(公告)日：2004-10-28
申请号：PCT/EP2004000771
申请日：2004-01-29
申请人： BASF PLANT SCIENCE GMBH , RENZ ANDREAS , HEINZ ERNST , ABBADI AMINE , DOMERGUE FREDERIC , ZANK THORSTEN
发明人： RENZ ANDREAS , HEINZ ERNST , ABBADI AMINE , DOMERGUE FREDERIC , ZANK THORSTEN
IPC分类号： C12N9/10 , C12N15/82 , C12P7/64
CPC分类号： C12N15/8247 , C12N9/1029 , C12P7/6427 , C12P7/6472
摘要： The invention relates to a method for producing polyunsaturated fatty acids in an organism by introducing nucleic acids coding for polypeptides with acyl-CoA:lysophospholipid-acyl transferase activity into the organism. Advantageously, said nucleic acid sequences can optionally be expressed in the transgenic organism along with additional nucleic acid sequences coding for polypeptides of fatty acid metabolism or lipid metabolism biosynthesis. The invention also relates to the nucleic acid sequences, nucleic acid structures containing said nucleic acid sequences, vectors containing the inventive nucleic acid sequences, and/or the nucleic acid structures and transgenic organisms containing said nucleic acid sequences, nucleic acid structures, and/or vectors. The invention further relates to oils, lipids, and/or fatty acids that are produced according the inventive method, and the use thereof.
摘要翻译：本发明涉及一种用于生产在由核酸的生物体的多不饱和脂肪酸的方法被引入到生物体中，其用于与酰基辅酶A的多肽代码：溶血磷脂酰基转移酶酶活性的编码。有利地，这些核酸序列可以任选地与编码脂肪酸或脂质代谢的生物合成的多肽的其他核酸序列一起在转基因生物体中表达。本发明还涉及核酸序列，含有根据本发明的核酸序列的核酸构建体，包含核酸序列的载体和/或含有上述核酸序列的核酸构建体和转基因生物，核酸构建体和/或载体。本发明的另一部分涉及通过erfindungsgemäbetaen方法制备的油，脂质和/或脂肪酸及其用途。

7. 发明申请

WO2005063995A3 SUGAR AND LIPID METABOLISM REGULATORS IN PLANTS VI 审中-公开
标题翻译：植物中的糖和脂肪代谢调节剂六
公开(公告)号：WO2005063995A3
公开(公告)日：2005-10-20
申请号：PCT/IB2004004251
申请日：2004-12-22
申请人： BASF PLANT SCIENCE GMBH , OSWALD OLIVER , BAUER JORG , ZANK THORSTEN
发明人： OSWALD OLIVER , BAUER JORG , ZANK THORSTEN
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8247 , C12N15/8245
摘要： Isolated nucleic acids and proteins associated with lipid and sugar metabolism regulation are provided. In particular, lipid metabolism proteins (LMP) and encoding nucleic acids originating from Arabidopsis thaliana, Brassica napus, and Helianthus annuus are provided. The nucleic acids and proteins are used in methods of producing transgenic plants and modulating levels of seed storage compounds. Preferably, the seed storage compounds are lipids, fatty acids, starches, or seed storage proteins.
摘要翻译：提供了与脂质和糖代谢调节相关的分离的核酸和蛋白质。特别地，提供脂质代谢蛋白（LMP）和编码来自拟南芥，甘蓝型油菜和向日葵的核酸。核酸和蛋白质用于产生转基因植物和调节种子储存化合物水平的方法中。优选地，种子储存化合物是脂质，脂肪酸，淀粉或种子储存蛋白。

8. 发明申请

WO2009027335A3 POLYPEPTIDES, SUCH AS LIPASES, CAPABLE OF ALTERING THE SEED STORAGE CONTENT IN TRANSGENIC PLANTS 审中-公开
标题翻译：多糖，如转基因，能够改变转基因植物中的种子储存内容
公开(公告)号：WO2009027335A3
公开(公告)日：2009-05-28
申请号：PCT/EP2008061014
申请日：2008-08-22
申请人： BASF PLANT SCIENCE GMBH , ZANK THORSTEN , OSWALD OLIVER , WETJEN TOM
发明人： ZANK THORSTEN , OSWALD OLIVER , WETJEN TOM
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8247 , C12N9/18
摘要： This invention relates generally to nucleic acid sequences encoding proteins that are related to the presence of seed storage compounds in plants. More specifically, the present invention relates to nucleic acid sequences encoding polypeptides being capable of altering the seed storage content and, in particular, oil, lipid and fatty acid metabolism regulator proteins and the use of these sequences in transgenic plants. In particular, the invention is directed to methods for manipulating seed storage compounds in plants and seeds. The invention further relates to methods of using these novel plant polypeptides to stimulate plant growth and/or to increase yield and/or composition of seed storage compounds.
摘要翻译：本发明一般涉及编码与种子储存化合物在植物中的存在有关的蛋白质的核酸序列。更具体地，本发明涉及编码能够改变种子储存含量，特别是油，脂质和脂肪酸代谢调节蛋白的多肽的核酸序列，以及这些序列在转基因植物中的用途。特别地，本发明涉及在植物和种子中操作种子储存化合物的方法。本发明还涉及使用这些新型植物多肽刺激植物生长和/或增加种子储存化合物的产量和/或组成的方法。

9. 发明申请

WO2006125756A2 NUCLEIC ACID MOLECULES ENCODING ECHI-LIKE POLYPEPTIDES AND METHODS OF USE 审中-公开
标题翻译：编码类似多聚核苷酸的核酸分子及其使用方法
公开(公告)号：WO2006125756A2
公开(公告)日：2006-11-30
申请号：PCT/EP2006062471
申请日：2006-05-19
申请人： BASF PLANT SCIENCE GMBH , ZANK THORSTEN , OSWALD OLIVER
发明人： ZANK THORSTEN , OSWALD OLIVER
IPC分类号： C12N15/82 , A01H5/00 , C12N15/29 , C12N15/60 , C12N15/61
CPC分类号： C12N15/8247 , C07K14/415 , C12N9/88 , C12N9/90
摘要： Described herein are inventions in the field of genetic engineering of plants, including isolated nucleic acid molecules encoding putative peroxisomal enoyl CoA hydratase/isomerase (ECHI) to improve agronomic, horticultural, and quality traits. This invention relates generally to nucleic acid sequences encoding proteins that are related to the presence of seed storage compounds in plants. More specifically, the present invention relates to ECHI-like nucleic acid sequences encoding lipid metabolism proteins (LMP) and the use of these sequences in transgenic plants. In particular, the invention is directed to methods for manipulating fatty acid-related compounds and for increasing oil level and altering the fatty acid composition in plants and seeds. The invention further relates to methods of using these novel plant polypeptides to stimulate plant growth and/or to increase yield and/or composition of seed storage compounds..
摘要翻译：本文描述了植物基因工程领域的发明，包括编码推定的过氧化物酶体烯酰辅酶A水合酶/异构酶（ECHI）的分离核酸分子，以改善农艺，园艺和品质性状。本发明一般涉及编码与种子储存化合物在植物中的存在有关的蛋白质的核酸序列。更具体地，本发明涉及编码脂质代谢蛋白（LMP）的ECHI样核酸序列和这些序列在转基因植物中的用途。特别地，本发明涉及用于操作脂肪酸相关化合物和增加油含量和改变植物和种子中的脂肪酸组成的方法。本发明还涉及使用这些新型植物多肽刺激植物生长和/或增加种子储存化合物的产量和/或组成的方法。

10. 发明申请

WO2009077545A3 LIPID METABOLISM PROTEIN AND USES THEREOF I (BZIP TRANSCRIPTION FACTOR) 审中-公开
标题翻译：脂质代谢蛋白及其用途（BZIP转录因子）
公开(公告)号：WO2009077545A3
公开(公告)日：2009-09-17
申请号：PCT/EP2008067678
申请日：2008-12-17
申请人： BASF PLANT SCIENCE GMBH , ZANK THORSTEN , HAERTEL HEIKO A , MITTENDORF VOLKER
发明人： ZANK THORSTEN , HAERTEL HEIKO A , MITTENDORF VOLKER
IPC分类号： C12N15/82 , A01H5/00 , C07K14/415 , C07K14/47
CPC分类号： C07K14/415 , C12N15/8245 , C12N15/8247
摘要： The present invention relates to a bZip transcription factor encoding polynucleotides and polypeptides. Further, the present invention relates to the use of the aforementioned nucleic acid sequences and proteins in transgenic plants. In particular, the invention is directed to methods for manipulating sugar-related compounds and for increasing oil level and altering the fatty acid composition in plants and seeds. The invention further relates to methods of using these novel plant polypeptides to stimulate plant growth and/or to increase yield and/or composition of seed storage compounds.
摘要翻译：本发明涉及编码多核苷酸和多肽的bZip转录因子。此外，本发明涉及上述核酸序列和蛋白质在转基因植物中的用途。特别地，本发明涉及用于操纵糖相关化合物和增加油含量和改变植物和种子中的脂肪酸组成的方法。本发明还涉及使用这些新型植物多肽刺激植物生长和/或增加种子储存化合物的产量和/或组成的方法。

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