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1. 发明授权

US4186052A NADH Peroxidase for hydrogen peroxide determination 失效
标题翻译： NADH过氧化物酶测定过氧化氢
公开(公告)号：US4186052A
公开(公告)日：1980-01-29
申请号：US811422
申请日：1977-06-29
申请人： Albert Roder , Hans Mollering , Wolfgang Gruber , Klaus Beaucamp , Hans Seidel , Peter Stahl , Detlef von Hoerschelmann
发明人： Albert Roder , Hans Mollering , Wolfgang Gruber , Klaus Beaucamp , Hans Seidel , Peter Stahl , Detlef von Hoerschelmann
IPC分类号： C12N9/08 , C12Q1/28 , G01N33/50 , C07G7/02 , C12D13/10 , G01N31/14
CPC分类号： C12N9/0065 , C12Q1/28
摘要： A novel peroxidase for reduced nicotinamide-adenine-dinucleotide is provided which peroxidase oxidizes reduced nicotinamide-adenine-dinucleotide with hydrogen peroxide to give nicotinamide-adenine-dinucleotide and water and is characterized by a Michaelis constant K.sub.Mto hydrogen peroxide of 2.8.times.10.sup.-5 M andto reduced nicotinamide-adenine-dinucleotide of 1.7.times.10.sup.-5 M,measured at 25.degree. C. in 0.2M tris buffer of pH 6.0, containing 0.1M potassium acetate. The peroxidase can be prepared by liberating the enzyme from Streptococcus faecalis ATCC 8043 by digestion or by treatment with a surface-active agent and isolating same from the enzyme solution obtained. Hydrogen peroxide is determined in a simple reaction or in a coupled reaction with a specific oxidase by contacting the said peroxidase with the H.sub.2 O.sub.2 producing reaction mixture at a pH of from 6.0 to 9.0 and measuring the change of extinction as a measure of H.sub.2 O.sub.2 and of the concentration of nicotinamide-adenine-dinucleotide initially present in said reaction mixture.
摘要翻译：提供了一种用于还原型烟酰胺 - 腺嘌呤二核苷酸的新型过氧化物酶，其过氧化物酶用过氧化氢氧化还原型烟酰胺 - 腺嘌呤二核苷酸，得到烟酰胺 - 腺嘌呤二核苷酸和水，其特征在于具有2.8x10-5M的过氧化氢的米氏常数KM，降低了1.7×10-5M的烟酰胺 - 腺嘌呤二核苷酸，在25℃下，在含有0.1M醋酸钾的0.2M Tris缓冲液pH6.0中测定。过氧化物酶可以通过消化或通过用表面活性剂处理从粪链球菌ATCC 8043中释放酶制备，并从获得的酶溶液中分离。过氧化氢在简单的反应或与特定氧化酶的偶联反应中通过使所述过氧化物酶与产生H 2 O 2的反应混合物在6.0至9.0的pH下接触并测定作为H 2 O 2和最初存在于所述反应混合物中的烟酰胺 - 腺嘌呤二核苷酸的浓度。

2. 发明授权

US4397952A Process for obtaining glucose dehydrogenase and micro-organism therefor 失效
标题翻译：获得葡萄糖脱氢酶及其微生物的方法
公开(公告)号：US4397952A
公开(公告)日：1983-08-09
申请号：US260480
申请日：1981-05-04
申请人： Detlef von Hoerschelmann , Hans Seidel , Gerhard Berger , Armin Masuth , Klaus Beaucamp , Wolfgang Gruber
发明人： Detlef von Hoerschelmann , Hans Seidel , Gerhard Berger , Armin Masuth , Klaus Beaucamp , Wolfgang Gruber
IPC分类号： C12N1/20 , C12N9/04 , C12Q1/54 , C12Q1/32 , C12R1/085
CPC分类号： C12N9/0006 , C12Q1/54 , C12R1/085 , Y10S435/834
摘要： The present invention provides a process for obtaining glucose dehydrogenase by culturing a micro-organism in an appropriate nutrient medium and obtaining the enzyme from the cell mass or from the culture medium, wherein the micro-organism cultured is Bacillus cereus DSM 1644. The nutrient medium used contains a carbon source which consists predominantly or solely of glycerol.
摘要翻译：本发明提供了通过在合适的营养培养基中培养微生物并从细胞块或培养基获得酶的方法，其中培养的微生物是蜡状芽孢杆菌DSM 1644.营养培养基使用的碳源主要或仅由甘油组成。

3. 发明授权

US4343903A Process for obtaining cholesterol esterase from micro-organisms 失效
标题翻译：从微生物获得胆固醇酯酶的方法
公开(公告)号：US4343903A
公开(公告)日：1982-08-10
申请号：US175808
申请日：1980-08-06
申请人： Klaus Beaucamp , Michael Nelboeck , Helmgard Gauhl , Hans Seidel , Wolfgang Gruber , Herwig Brunner
发明人： Klaus Beaucamp , Michael Nelboeck , Helmgard Gauhl , Hans Seidel , Wolfgang Gruber , Herwig Brunner
IPC分类号： C12N1/14 , C12N1/20 , C12N9/18
CPC分类号： C12N9/18
摘要： The present invention provides a process for obtaining cholesterol esterase from micro-organisms, wherein a micro-organism capable of cholesterol esterase formation is cultured in an appropriate nutrient medium in the presence of lecithin as inducer and the enzyme is obtained from the culture medium and/or from the cells.
摘要翻译：本发明提供从微生物获得胆固醇酯酶的方法，其中在卵磷脂作为诱导剂的存在下，在合适的营养培养基中培养能够形成胆固醇酯酶的微生物，并且从培养基和/ 或从细胞。

4. 发明授权

US4360596A Process for the preparation of cholesterol esterase 失效
标题翻译：胆固醇酯酶的制备方法
公开(公告)号：US4360596A
公开(公告)日：1982-11-23
申请号：US175809
申请日：1980-08-06
申请人： Klaus Beaucamp , Michael Nelboeck , Helmgard Gauhl , Hans Seidel , Wolfgang Gruber , Herwig Brunner
发明人： Klaus Beaucamp , Michael Nelboeck , Helmgard Gauhl , Hans Seidel , Wolfgang Gruber , Herwig Brunner
IPC分类号： C12N9/18 , C12Q1/60
CPC分类号： C12N9/18 , C12Q1/60 , Y10S435/874
摘要： The present invention provides a process for obtaining cholesterol esterase from micro-organisms of the genus Pseudomonas, wherein Pseudomonas sp. DSM 1280 or DSM 1281 is cultured in an appropriate culture medium in the presence of an inducer and the enzyme is obtained from the culture medium and/or from the cells.
摘要翻译：本发明提供了从假单胞菌属的微生物获得胆固醇酯酶的方法，其中假单胞菌属 DSM 1280或DSM 1281在诱导剂存在下在合适的培养基中培养，并且从培养基和/或从细胞获得酶。

5. 发明授权

US3951744A Purification of dehydrogenases 失效
标题翻译：脱氢酶的纯化
公开(公告)号：US3951744A
公开(公告)日：1976-04-20
申请号：US489792
申请日：1974-07-16
申请人： Albert Roder , Klaus Beaucamp , Gunter Weimann , Walter Schneider , Klaus Muhlegger
发明人： Albert Roder , Klaus Beaucamp , Gunter Weimann , Walter Schneider , Klaus Muhlegger
IPC分类号： C12N9/02 , C12N9/04 , C12N9/12 , C07G7/02
CPC分类号： C12N9/0006 , Y10S435/815
摘要： NADP-dependent dehydrogenases are separated and purified by subjecting mixtures containing it to affinity chromatography using as the affinity chromatography material a nucleotide with a phosphate group in the 2'- or 3'-position, bound to a solid carrier.
摘要翻译：通过使含有它的混合物的混合物进行亲和层析，使用与固体载体结合的2'-或3'-位上具有磷酸基团的核苷酸作为亲和层析材料，分离和纯化NADP依赖性脱氢酶。

6. 发明授权

US4008127A Process for the preparation of cholesterol oxidase 失效
标题翻译：胆固醇氧化酶的制备方法
公开(公告)号：US4008127A
公开(公告)日：1977-02-15
申请号：US633997
申请日：1975-11-20
申请人： Wolfgang Gruber , Hans Ulrich Bergmeyer , Michael Nelboeck-Hochstetter , Klaus Beaucamp , Gunter Holz , Johanna Gramsall , Gunter Lang
发明人： Wolfgang Gruber , Hans Ulrich Bergmeyer , Michael Nelboeck-Hochstetter , Klaus Beaucamp , Gunter Holz , Johanna Gramsall , Gunter Lang
IPC分类号： C07J9/00 , C12N9/04 , C12D13/10
CPC分类号： C12N9/0006 , Y10S435/822 , Y10S435/872
摘要： Cholesterol oxidase which oxidizes cholesterol with oxygen to give cholestenone and hydrogen peroxide, is prepared by culturing Nocardia erthropolis ATCC 17895, Nocardia erthropolis ATCC 4277, Nocardia formica ATCC 14811 or Proactinomyce erythropolis NCIB 9158 first on a peptone-containing mineral salt medium and thereafter on at least one sterol compound as the sole source of carbon, and then isolating the cholesterol oxidase formed from the cultured micro-organism, wherein said sterol compound is of the formula ##STR1## in which R.sub.1 and R.sub.2 are hydrogen or together represent a double bond,R.sub.3 and R.sub.4 are hydrogen or together represent a double bond, andR.sub.5 is hydrogen or alkyl containing up to 3 carbon atoms.
摘要翻译：首先通过在含蛋白胨的矿物盐介质上培养诺卡氏菌霍奇金菌ATCC 17895，诺卡氏菌诺卡氏菌诺丝诺菌ATCC 4277，诺卡氏菌ATCC 14811或Proactinomyce erythropolis NCIB 9158来制备胆固醇氧化胆固醇以产生胆碱酯酶和过氧化氢的胆固醇氧化酶，至少一种固醇化合物作为唯一的碳源，然后分离由培养的微生物形成的胆固醇氧化酶，其中所述甾醇化合物具有式（I），其中R 1和R 2是氢或一起代表双键，R 3和R 4是氢或一起表示双键，并且R 5是氢或含有至多3个碳原子的烷基。

7. 发明授权

US4212938A Reagent and method for the determination of cholesterol 失效
标题翻译：用于测定胆固醇的试剂和方法
公开(公告)号：US4212938A
公开(公告)日：1980-07-15
申请号：US360515
申请日：1973-05-15
申请人： Wolfgang Gruber , Hans U. Bergmeyer , Erich Bernt , Alexander Hagen , Peter Roeschlau , Gunter Lang , Klaus Beaucamp
发明人： Wolfgang Gruber , Hans U. Bergmeyer , Erich Bernt , Alexander Hagen , Peter Roeschlau , Gunter Lang , Klaus Beaucamp
IPC分类号： G01N33/92 , A61B10/00 , C07J9/00 , C12Q1/26 , C12Q1/60 , G01N33/15 , G01N33/48 , C12Q1/50
CPC分类号： C12Q1/60
摘要： Cholesterol is determined by incubating a sample suspected of containing cholesterol in an aqueous medium with cholesterol oxidase and determining either the oxygen consumption, the hydrogen peroxide formed, or cholestenone, as a measure of the initial cholesterol content; reagent compositions comprising cholesterol oxidase and a system for the determination of H.sub.2 O.sub.2 or for the determination of cholestenone are provided.
摘要翻译：胆固醇通过将怀疑含有胆固醇的样品置于水性介质中与胆固醇氧化酶并测定氧消耗，形成的过氧化氢或胆碱酯酶作为初始胆固醇含量的量度来确定; 提供了包含胆固醇氧化酶的试剂组合物和用于测定H 2 O 2或测定胆碱酯的系统。

8. 发明授权

US4164448A Activation of cholesterol oxidase for cholesterol assay 失效
标题翻译：胆固醇氧化酶激活胆固醇测定
公开(公告)号：US4164448A
公开(公告)日：1979-08-14
申请号：US768530
申请日：1977-02-14
申请人： Peter Roeschlau , Gunter Lang , Klaus Beaucamp , Erich Bernt , Wolfgang Gruber
发明人： Peter Roeschlau , Gunter Lang , Klaus Beaucamp , Erich Bernt , Wolfgang Gruber
IPC分类号： C12N9/04 , C12Q1/60 , C07G7/02 , G01N31/14
CPC分类号： C12N9/0006 , C12Q1/60
摘要： Cholesterol oxidase free of unstabilizing residual traces of detergent is activated by adding to the cholesterol oxidase, before use thereof, at least one surface-active compound with lipophilic and hydrophilic properties. Diagnostic agents for determining cholesterol comprising cholesterol oxidase, a hydrogen peroxide detection system, a buffer, and at least one surface-active compound having lipophilic and hydrophilic properties are provided.
摘要翻译：没有不稳定残留痕量的洗涤剂的胆固醇氧化酶通过在使用前加入至少一种具有亲油性和亲水性的表面活性化合物来激活胆固醇氧化酶。提供了用于测定胆固醇的诊断剂，其包含胆固醇氧化酶，过氧化氢检测系统，缓冲液和至少一种具有亲油性和亲水性的表面活性化合物。

9. 发明授权

US4144129A Cholesteroloxidase and method for its production from microorganisms 失效
标题翻译：胆固醇酶及其从微生物产生的方法
公开(公告)号：US4144129A
公开(公告)日：1979-03-13
申请号：US360490
申请日：1973-05-15
申请人： Wolfgang Gruber , Hans U. Bergmeyer , Michael Nelbock-Hochstetter , Klaus Beaucamp , Gunter Holz , Johanna Gramsall , Gunter Lang
发明人： Wolfgang Gruber , Hans U. Bergmeyer , Michael Nelbock-Hochstetter , Klaus Beaucamp , Gunter Holz , Johanna Gramsall , Gunter Lang
IPC分类号： G01N33/92 , C12N9/04 , C12Q1/26 , C12Q1/60 , C12R1/01 , C12R1/325 , C12R1/365 , C12R1/645 , C07G7/028
CPC分类号： C12N9/0006 , C12Q1/60 , Y10S435/814 , Y10S435/826 , Y10S435/872
摘要： A new enzyme, cholesteroloxidase, is prepared by recovering same from microorganisms, preferably by washing a cholesterol-converting microorganism with butanol/water, disintegrating the microorganism culture and extracting same with a buffer solution containing a non-ionic surface-active agent, and isolating cholesterol oxidase from the extract.
摘要翻译：通过从微生物中回收相应的新酶胆固醇氧化酶，优选通过用丁醇/水洗涤胆固醇转化微生物，分解微生物培养物并用含有非离子表面活性剂的缓冲液进行提取，并分离来自提取物的胆固醇氧化酶。

10. 发明授权

US4334023A Process for obtaining cholesterol oxidase 失效
标题翻译：获得胆固醇氧化酶的方法
公开(公告)号：US4334023A
公开(公告)日：1982-06-08
申请号：US147460
申请日：1980-05-07
申请人： Helmgard Gauhl , Georg Schawohl , Hans Seidel , Klaus Beaucamp
发明人： Helmgard Gauhl , Georg Schawohl , Hans Seidel , Klaus Beaucamp
IPC分类号： C12N9/08 , C12N9/04 , C12R1/06 , C12R1/465
CPC分类号： C12N9/0006 , Y10S435/83 , Y10S435/886 , Y10S435/898
摘要： The present invention provides a process for obtaining cholesterol oxidase, wherein Streptomyces griseofuscus DSM 40191, Streptomyces hygroscopicus 40771, Streptomyces acidomyceticus DSM 40798 and/or Arthrobacter paraffinens DSM 312 are cultured and the enzyme obtained from the culture supernatant and/or the cells.
摘要翻译：本发明提供一种获得胆固醇氧化酶的方法，其中培养灰霉菌（Streptomyces griseofuscus）DSM 40191，吸水链霉菌40771，链霉菌酸性霉菌DSM 40798和/或节杆菌属链烷烃DSM 312，以及从培养上清液和/或细胞获得的酶。

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