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1. 发明授权

US4212938A Reagent and method for the determination of cholesterol 失效
标题翻译：用于测定胆固醇的试剂和方法
公开(公告)号：US4212938A
公开(公告)日：1980-07-15
申请号：US360515
申请日：1973-05-15
申请人： Wolfgang Gruber , Hans U. Bergmeyer , Erich Bernt , Alexander Hagen , Peter Roeschlau , Gunter Lang , Klaus Beaucamp
发明人： Wolfgang Gruber , Hans U. Bergmeyer , Erich Bernt , Alexander Hagen , Peter Roeschlau , Gunter Lang , Klaus Beaucamp
IPC分类号： G01N33/92 , A61B10/00 , C07J9/00 , C12Q1/26 , C12Q1/60 , G01N33/15 , G01N33/48 , C12Q1/50
CPC分类号： C12Q1/60
摘要： Cholesterol is determined by incubating a sample suspected of containing cholesterol in an aqueous medium with cholesterol oxidase and determining either the oxygen consumption, the hydrogen peroxide formed, or cholestenone, as a measure of the initial cholesterol content; reagent compositions comprising cholesterol oxidase and a system for the determination of H.sub.2 O.sub.2 or for the determination of cholestenone are provided.
摘要翻译：胆固醇通过将怀疑含有胆固醇的样品置于水性介质中与胆固醇氧化酶并测定氧消耗，形成的过氧化氢或胆碱酯酶作为初始胆固醇含量的量度来确定; 提供了包含胆固醇氧化酶的试剂组合物和用于测定H 2 O 2或测定胆碱酯的系统。

2. 发明授权

US4164448A Activation of cholesterol oxidase for cholesterol assay 失效
标题翻译：胆固醇氧化酶激活胆固醇测定
公开(公告)号：US4164448A
公开(公告)日：1979-08-14
申请号：US768530
申请日：1977-02-14
申请人： Peter Roeschlau , Gunter Lang , Klaus Beaucamp , Erich Bernt , Wolfgang Gruber
发明人： Peter Roeschlau , Gunter Lang , Klaus Beaucamp , Erich Bernt , Wolfgang Gruber
IPC分类号： C12N9/04 , C12Q1/60 , C07G7/02 , G01N31/14
CPC分类号： C12N9/0006 , C12Q1/60
摘要： Cholesterol oxidase free of unstabilizing residual traces of detergent is activated by adding to the cholesterol oxidase, before use thereof, at least one surface-active compound with lipophilic and hydrophilic properties. Diagnostic agents for determining cholesterol comprising cholesterol oxidase, a hydrogen peroxide detection system, a buffer, and at least one surface-active compound having lipophilic and hydrophilic properties are provided.
摘要翻译：没有不稳定残留痕量的洗涤剂的胆固醇氧化酶通过在使用前加入至少一种具有亲油性和亲水性的表面活性化合物来激活胆固醇氧化酶。提供了用于测定胆固醇的诊断剂，其包含胆固醇氧化酶，过氧化氢检测系统，缓冲液和至少一种具有亲油性和亲水性的表面活性化合物。

3. 发明授权

US3948728A Steroid-delta-isomerase 失效
标题翻译：类固醇-δ-异构酶
公开(公告)号：US3948728A
公开(公告)日：1976-04-06
申请号：US566481
申请日：1975-04-09
申请人： Peter Roeschlau , Gunter Lang , Klaus Beaucamp , Erich Bernt
发明人： Peter Roeschlau , Gunter Lang , Klaus Beaucamp , Erich Bernt
IPC分类号： C12N9/90 , C12P33/00 , C07G7/028 , C12D13/10 , C12K1/00
CPC分类号： C12N9/90 , Y10S435/814 , Y10S435/815 , Y10S435/872
摘要： The new enzyme steroid-.DELTA.-isomerase is provided, as well as a process for producing same, comprising digesting a micro-organism containing said isomerase, extracting same by destruction of the cell wall with a buffer solution containing a non-ionic surfactant, centrifuging the resulting mass and discarding the precipitate, applying the extract to an anion exchanger, eluting the enzyme with a buffer solution containing a non-ionic surfactant, heating the eluate, separating the precipitate and isolating the enzyme therefrom from the supernatant.
摘要翻译：提供新的酶类固醇-DTA-异构酶，以及其生产方法，包括消化含有所述异构酶的微生物，通过用含有非离子表面活性剂的缓冲溶液破坏细胞壁，将其离心，产生的质量并丢弃沉淀物，将提取物施加到阴离子交换剂上，用含有非离子表面活性剂的缓冲溶液洗脱酶，加热洗脱液，分离沉淀并从上清液中分离出酶。

4. 发明授权

US4184921A Process and reagent for determining cholesterol 失效
标题翻译：用于测定胆固醇的方法和试剂
公开(公告)号：US4184921A
公开(公告)日：1980-01-22
申请号：US900958
申请日：1978-04-28
申请人： Peter Roeschlau , Erich Bernt , Wolfgang Gruber
发明人： Peter Roeschlau , Erich Bernt , Wolfgang Gruber
IPC分类号： C12Q1/60 , G01N31/14
CPC分类号： C12Q1/60
摘要： Cholesterol is determined utilizing cholesterol oxidase, and optionally cholesterol esterase to cleave bound cholesterol, in the presence of peroxidase, p-amino-phenazone, a surface-active agent and phenol, the improvement comprising employing an alkanol of up to 4 carbon atoms in the test composition or sample to substantially overcome test error and deviations due to turbidity formation.
摘要翻译：在过氧化物酶，对氨基 - 吩噻嗪，表面活性剂和苯酚的存在下，使用胆固醇氧化酶和任选的胆固醇酯酶来切割结合的胆固醇来确定胆固醇，其改进包括使用最多4个碳原子的链烷醇测试组合物或样品以基本上克服由于浊度形成引起的测试误差和偏差。

5. 发明授权

US4181575A Composition and method for the determination of cholesterol 失效
标题翻译：胆固醇测定的组成和方法
公开(公告)号：US4181575A
公开(公告)日：1980-01-01
申请号：US842001
申请日：1977-10-13
申请人： Wolfgang Gruber , Gunter Lang , Michael Nelboeck-Hochstetter , Peter Roeschlau , Hans Seidel , Detlef von Hoerschelmann
发明人： Wolfgang Gruber , Gunter Lang , Michael Nelboeck-Hochstetter , Peter Roeschlau , Hans Seidel , Detlef von Hoerschelmann
IPC分类号： G01N33/92 , C12N9/04 , C12Q1/60 , G01N31/14
CPC分类号： C12N9/0006 , C12Q1/60
摘要： In a process for the determination of total or bound cholesterol by liberating any bound cholesterol using cholesterol esterase and simultaneously or subsequently determining the liberated cholesterol and any initially free cholesterol with a cholesterol reacting enzyme, the improvement comprising using as the cholesterol reacting enzyme an NAD or NADP-dependent cholesterol dehydrogenase obtained from an anaerobic microorganism, e.g., Eubacterium sp. ATCC 21408, or from mammalian tissue, e.g., liver.
摘要翻译：在通过使用胆固醇酯酶释放任何结合的胆固醇并同时或随后使用胆固醇反应酶确定释放的胆固醇和任何初始游离胆固醇来测定总胆固醇或结合胆固醇的方法中，该改进包括将胆固醇反应酶用作NAD或从厌氧微生物获得的NADP依赖性胆固醇脱氢酶，例如真杆菌属 ATCC 21408，或来自哺乳动物组织，例如肝脏。

6. 发明授权

US4123384A Control serum containing alkaline phosphatase of constant activity 失效
标题翻译：控制含有碱性磷酸酶活性的血清
公开(公告)号：US4123384A
公开(公告)日：1978-10-31
申请号：US715021
申请日：1976-08-16
申请人： Dieter Hundt , Wolfgang Gruber , Michael Klarwein , Peter Roeschlau
发明人： Dieter Hundt , Wolfgang Gruber , Michael Klarwein , Peter Roeschlau
IPC分类号： C12N9/16 , C12N9/96 , C12Q1/42 , C09K3/00 , G01N31/14 , G01N33/16
CPC分类号： C12Q1/42 , C12N9/16 , C12N9/96 , Y10T436/106664
摘要： The activity of alkaline phosphatase in aqueous solution, especially in reconstituted serum, is kept constant by adding to the phosphatase-containing solution Mn.sup.2+ or Cd.sup.2+ ions, preferably to an end concentration of 0.05 to 0.5 mMol/liter of Mn.sup.2+ or 0.05 to 0.1 mMol/liter of Cd.sup.2+. Control serums comprising the Mn.sup.2+ and/or Cd.sup.2+ ions in human or animal serum or serum-like compositions are also provided.
摘要翻译：碱性磷酸酶在水溶液中，特别是重构血清中的活性通过加入含磷酸酶的溶液Mn2 +或Cd2 +离子而保持恒定，优选终浓度为0.05至0.5mMol /升的Mn 2+或0.05至0.1mMol / 升Cd2 +。还提供了在人或动物血清或血清样组合物中控制含有Mn 2+和/或Cd 2+离子的血清。

7. 发明授权

US4168205A Method for the determination of substrates or enzyme activities 失效
标题翻译：测定底物或酶活性的方法
公开(公告)号：US4168205A
公开(公告)日：1979-09-18
申请号：US792073
申请日：1977-04-28
申请人： Josef Danninger , Ulfert Deneke , Gunter Lang , Gerhard Michal , Peter Roeschlau
发明人： Josef Danninger , Ulfert Deneke , Gunter Lang , Gerhard Michal , Peter Roeschlau
IPC分类号： G01N33/50 , C12Q1/26 , C12Q1/54 , G01N33/16
CPC分类号： C12Q1/54 , C12Q1/26
摘要： In a process for the determination of substrates or enzyme activities in a test batch, utilizing a conventional redox reaction as a measurement reaction, the improvement comprising carrying out the process in the presence of ascorbate oxidase; the addition of ascorbate oxidase substantially eliminates the interfering effect of ascorbic acid frequently found in the test sample.
摘要翻译：在测定批次中测定底物或酶活性的方法中，利用常规氧化还原反应作为测量反应，改进包括在存在抗坏血酸氧化酶的情况下进行该方法; 抗坏血酸氧化酶的添加基本上消除了测试样品中常见的抗坏血酸的干扰作用。

8. 发明授权

US4409327A Process and reagent for the determination of the biological activity of heparin in plasma 失效
标题翻译：用于测定血浆中肝素生物活性的方法和试剂
公开(公告)号：US4409327A
公开(公告)日：1983-10-11
申请号：US233536
申请日：1981-02-11
申请人： Knut Bartl , Helmut Lill , Peter Roeschlau , Joachim Ziegenhorn
发明人： Knut Bartl , Helmut Lill , Peter Roeschlau , Joachim Ziegenhorn
IPC分类号： G01N33/66 , C12Q1/37 , C12Q1/56 , G01N33/50 , G01N33/86 , C12Q1/38
CPC分类号： G01N33/86 , C12Q2337/12 , G01N2333/8117 , G01N2333/96444 , G01N2333/974 , G01N2400/40 , Y10S435/81
摘要： Method and reagent for the determination of the biological activity of heparin in plasma by adding thrombin or Factor X.sub.a as the proteolytic enzyme and a chromogen substrate of the latter and measuring the dye liberated from the chromogen substrate in the absence of exogenous anti-thrombin III, the improvement consisting of adding, additionally, at least one compound of the formula ##STR1## wherein X is oxygen or the group NR.sub.5 ;and R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 are independently selected from hydrogen or alkyl of 1 to 2 carbon atoms.
摘要翻译：通过添加凝血酶或因子Xa作为蛋白水解酶和后者的色底物，测定血浆中肝素生物活性的方法和试剂，并测定在没有外源性抗凝血酶III条件下从色底物释放的染料，该改进包括另外加入至少一种式（IMAGE）化合物，其中X是氧或NR5基团; 并且R 1，R 2，R 3，R 4和R 5独立地选自氢或1至2个碳原子的烷基。

9. 发明授权

US4692406A Process and a reagent for the simultaneous determination of fibrinogen and fibrinogen fission products in plasma 失效
标题翻译：用于同时测定血浆中纤维蛋白原和纤维蛋白原裂变产物的方法和试剂
公开(公告)号：US4692406A
公开(公告)日：1987-09-08
申请号：US642421
申请日：1984-08-20
申请人： Udo Becker , Peter Roeschlau
发明人： Udo Becker , Peter Roeschlau
IPC分类号： C12Q1/56 , G01N33/86
CPC分类号： C12Q1/56 , G01N2333/4623 , G01N2333/4626 , G01N2333/75 , G01N2333/974 , Y10S435/81
摘要： The present invention provides a process for the simultaneous determination of fibrinogen and fibrinogen fission products in plasma, wherein 0.01 to 1 U of a snake venom enzyme with a thrombin-like effectiveness is aded per ml. of sample plasma, then there is measured the period of time between the addition of the enzyme and the commencement of a turbidity formation as a measure for the amount of fibrinogen fission products and subsequently there is measured the speed of the turbidity formation as a measure of the amount of fibrinogen.The present invention also provides a reagent for the simultaneous determination of fibrinogen and fibrinogen fission products, wherein it comprises 0.01 to 10 U snake venon enzyme with thrombin-like effectiveness per ml. of sample plasma, 0.5 to 4% by weight of a water-soluble polyalcohol, 1 to 20 mMole/liter of calcium ions and 0.05 to 0.3 mole/liter of buffer of pH 7.0 to 8.0, referred to the solution ready for use, as well as optionally a detergent.
摘要翻译：本发明提供了一种用于同时测定血浆中的纤维蛋白原和纤维蛋白原裂变产物的方法，其中每ml给予0.01至1U具有凝血酶样效力的蛇毒液。的样品血浆，然后测量添加酶和开始浑浊形成之间的时间段作为纤维蛋白原裂变产物量的量度，随后测量浊度形成的速度作为纤维蛋白原的量。本发明还提供了一种用于同时测定纤维蛋白原和纤维蛋白原裂变产物的试剂，其中它含有每毫升具有凝血酶样有效性的0.01至10U蛇毒素酶。的样品血浆，0.5至4重量％的水溶性多元醇，1至20mMole /升钙离子和0.05至0.3摩尔/升pH 7.0至8.0的缓冲液，称为待使用的溶液，为以及任选的洗涤剂。

10. 发明授权

US4429040A Method and reagent for the detection of fibrin monomer 失效
标题翻译：检测纤维蛋白单体的方法和试剂
公开(公告)号：US4429040A
公开(公告)日：1984-01-31
申请号：US256212
申请日：1981-04-21
申请人： Udo Becker , Peter Roeschlau
发明人： Udo Becker , Peter Roeschlau
IPC分类号： G01N33/68 , G01N33/86 , G01N33/54 , C12Q1/56
CPC分类号： G01N33/86 , G01N2333/75 , Y10S435/962
摘要： The present invention provides a process for the detection or determination of fibrin monomer in biological fluids, wherein the sample fluid is incubated with hydrophobic latex and the agglutination determined.The present invention also provides a reagent for the detection or determination of fibrin or fibrin monomer in biological fluids, wherein it contains hydrophobic latex.
摘要翻译：本发明提供了用于检测或测定生物流体中的纤维蛋白单体的方法，其中将样品流体与疏水胶乳一起温育并确定凝集。本发明还提供了用于检测或测定生物流体中纤维蛋白或纤维蛋白单体的试剂，其中它含有疏水胶乳。

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