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    • 1. 发明授权
    • Double-stranded nucleic acid molecule for gene expression control
    • 用于基因表达控制的双链核酸分子
    • US09593331B2
    • 2017-03-14
    • US14355711
    • 2012-11-01
    • Akira TachibanaToshizumi Tanabe
    • Akira TachibanaToshizumi Tanabe
    • C12N15/11C12N15/113A61K31/7105
    • C12N15/113A61K31/7105C12N15/111C12N2310/11C12N2310/14C12N2310/531C12N2320/50C12N2320/51
    • Provided are an improved double-stranded nucleic acid molecule involved in gene expression control mediated by a gene silencing mechanism, a method of producing the molecule, and a pharmaceutical composition comprising the double-stranded nucleic acid molecule. The double-stranded nucleic acid molecule for gene expression control comprises an antisense strand having a length of 18 to 28 nucleotides and a sense strand including a complementary moiety composed of a sequence sufficiently complementary to the antisense strand and a protruding single-stranded 5′-end moiety having a length of 2 to 100 nucleotides. The sense strand and the antisense strand form base pairs via the complementary moiety. The method produces such a double-stranded nucleic acid molecule, and the pharmaceutical composition contains such a double-stranded nucleic acid molecule as an active ingredient.
    • 提供涉及由基因沉默机制介导的基因表达控制的改进的双链核酸分子,产生该分子的方法和包含双链核酸分子的药物组合物。 用于基因表达控制的双链核酸分子包含长度为18至28个核苷酸的反义链和包含由与反义链足够互补的序列构成的互补部分的有义链和突出的单链5'- 末端部分具有2至100个核苷酸的长度。 有义链和反义链通过互补部分形成碱基对。 该方法产生这样的双链核酸分子,并且该药物组合物含有作为活性成分的这种双链核酸分子。