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1. 发明专利

JP2010019613A Cytokine-producing cell detection method 审中-公开
标题翻译：细胞因子生产细胞检测方法
公开(公告)号：JP2010019613A
公开(公告)日：2010-01-28
申请号：JP2008178604
申请日：2008-07-09
申请人： Ryo Tachibana , Toshizumi Tanabe , 利住田辺 , 亮立花
发明人： TANABE TOSHIZUMI , TACHIBANA RYO
IPC分类号： G01N33/53 , G01N33/543
摘要： PROBLEM TO BE SOLVED: To provide a simple method which specifies a position in a container of cells that produce cytokine.
SOLUTION: In this cytokine-producing cell detection method, a protein-adsorbing film dipped into a serum-free culture medium is placed on cultures cells adhering onto the surface of a solid surface, and cytokine produced from the cultures cells is adsorbed onto the protein-adsorbing film; and after cleaning the protein-adsorbing film, the cytokine is immersed into a primary antibody solution to the object cytokine, and reacted by using a secondary antibody, and then cytokine producing cells are detected.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供一种简单的方法，其指定产生细胞因子的细胞的容器中的位置。解决方案：在这种细胞因子产生细胞检测方法中，将浸入无血清培养基中的蛋白质吸附膜置于附着在固体表面的培养细胞上，并且从培养细胞产生的细胞因子被吸附到蛋白质吸附膜上; 在清洗蛋白质吸附膜之后，将细胞因子浸入到对象细胞因子的一次抗体溶液中，并通过使用二次抗体进行反应，然后检测细胞因子产生细胞。版权所有（C）2010，JPO＆INPIT

2. 发明授权

US09593331B2 Double-stranded nucleic acid molecule for gene expression control 有权
标题翻译：用于基因表达控制的双链核酸分子
公开(公告)号：US09593331B2
公开(公告)日：2017-03-14
申请号：US14355711
申请日：2012-11-01
申请人： Akira Tachibana , Toshizumi Tanabe
发明人： Akira Tachibana , Toshizumi Tanabe
IPC分类号： C12N15/11 , C12N15/113 , A61K31/7105
CPC分类号： C12N15/113 , A61K31/7105 , C12N15/111 , C12N2310/11 , C12N2310/14 , C12N2310/531 , C12N2320/50 , C12N2320/51
摘要： Provided are an improved double-stranded nucleic acid molecule involved in gene expression control mediated by a gene silencing mechanism, a method of producing the molecule, and a pharmaceutical composition comprising the double-stranded nucleic acid molecule. The double-stranded nucleic acid molecule for gene expression control comprises an antisense strand having a length of 18 to 28 nucleotides and a sense strand including a complementary moiety composed of a sequence sufficiently complementary to the antisense strand and a protruding single-stranded 5′-end moiety having a length of 2 to 100 nucleotides. The sense strand and the antisense strand form base pairs via the complementary moiety. The method produces such a double-stranded nucleic acid molecule, and the pharmaceutical composition contains such a double-stranded nucleic acid molecule as an active ingredient.
摘要翻译：提供涉及由基因沉默机制介导的基因表达控制的改进的双链核酸分子，产生该分子的方法和包含双链核酸分子的药物组合物。用于基因表达控制的双链核酸分子包含长度为18至28个核苷酸的反义链和包含由与反义链足够互补的序列构成的互补部分的有义链和突出的单链5'- 末端部分具有2至100个核苷酸的长度。有义链和反义链通过互补部分形成碱基对。该方法产生这样的双链核酸分子，并且该药物组合物含有作为活性成分的这种双链核酸分子。

3. 发明授权

US5098840A Human prourokinase mutants 失效
标题翻译：人类prourokinase突变体
公开(公告)号：US5098840A
公开(公告)日：1992-03-24
申请号：US525011
申请日：1990-05-18
申请人： Shunji Kasai , Ryuji Hiramatsu , Shusei Uno , Masanori Nagai , Hirofumi Arimura , Toshizumi Tanabe , Yasuo Amatsuji , Masaaki Hirose , Masanori Morita , Haruhide Kawabe
发明人： Shunji Kasai , Ryuji Hiramatsu , Shusei Uno , Masanori Nagai , Hirofumi Arimura , Toshizumi Tanabe , Yasuo Amatsuji , Masaaki Hirose , Masanori Morita , Haruhide Kawabe
IPC分类号： A61K38/00 , C12N9/72 , C12N15/58
CPC分类号： C12N9/6462 , C12Y304/21073 , A61K38/00
摘要： A human prourokinase mutant in which the entire or a partial epidermal growth factor domain of human prourokinase is deleted or a partial epidermal growth factor domain of human prourokinase is replaced by one or more different amino acid residues, said mutant having a longer blood half-life than naturally occurring human prourokinase while retaining prourokinase enzymatic activity. In this human prourokinase mutant the region selected from the group consisting of: (a) from asparagine (10) to cysteine (42); (b) from asparagine (10) to aspartic acid (45); and (c) from asparagine (10) to threonine (49) is missing.
摘要翻译：其中缺少人原尿激酶的全部或部分表皮生长因子结构域的人prourokinase突变体或人原尿激酶的部分表皮生长因子结构域被一个或多个不同的氨基酸残基替代，所述突变体具有较长的血液半衰期而不是天然存在的人类prourokinase，同时保留prourokinase酶活性。在该人类prourokinase突变体中，选自：（a）天冬酰胺（10）至半胱氨酸（42）的区域; （b）从天冬酰胺（10）到天冬氨酸（45）; 和（c）从天冬酰胺（10）到苏氨酸（49）缺失。

4. 发明授权

US5389538A Mutant human prourokinase 失效
标题翻译：突变型人类原尿激酶
公开(公告)号：US5389538A
公开(公告)日：1995-02-14
申请号：US957039
申请日：1992-10-06
申请人： Toshizumi Tanabe , Masanori Morita , Masaaki Hirose , Yasuo Amatsuji
发明人： Toshizumi Tanabe , Masanori Morita , Masaaki Hirose , Yasuo Amatsuji
IPC分类号： A61K38/00 , C12N9/72 , C12N15/09 , C12N15/58 , C12R1/91 , A61K37/547
CPC分类号： C12N9/6462 , C12Y304/21073 , A61K38/00
摘要： A mutant human prourokinase wherein a neutral amino acid in the epidermal growth factor (EGF) region of human prourokinase (human PUK) has been replaced with a basic amino acid, or an acidic amino acid has been replaced with a non-acidic amino acid, and a method for producing a mutant human PUK which comprises expression of mutant human PUK by cultivating a host transformed by a plasmid inserted with a DNA sequence coding for said mutant human PUK. By replacing a neutral amino acid in the EGF region of human PUK which is a fibrinolysin with a basic amino acid, or an acidic amino acid with a non-acidic amino acid, half-life in blood can be prolonged, and affinity for fibrin can be improved.
摘要翻译：其中人原尿激酶（人PUK）的表皮生长因子（EGF）区域中的中性氨基酸已被碱性氨基酸置换的突变型人类原尿激酶，或者酸性氨基酸被非酸性氨基酸替代，以及生产突变型人PUK的方法，其包括通过培养由插入有编码所述突变型人PUK的DNA序列的质粒转化的宿主来表达突变型人PUK。通过用碱性氨基酸或具有非酸性氨基酸的酸性氨基酸取代作为纤维蛋白溶素的人PUK的EGF区中的中性氨基酸，可以延长血液中的半衰期，并且对纤维蛋白的亲和力可以要改进

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