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1. 发明申请

WO2008121324A3 RECOMBINANT EXPRESSION VECTOR ELEMENTS (REVES) FOR ENHANCING EXPRESSION OF RECOMBINANT PROTEINS IN HOST CELLS 审中-公开
标题翻译：重组表达载体元件（改良）增强重组蛋白在宿主细胞中的表达
公开(公告)号：WO2008121324A3
公开(公告)日：2008-12-11
申请号：PCT/US2008004063
申请日：2008-03-28
申请人： ABBOTT LAB , GION WENDY R , CARSON GERALD R , GAO HONG , KUNES YUNE Z
发明人： GION WENDY R , CARSON GERALD R , GAO HONG , KUNES YUNE Z
IPC分类号： C07H21/04
CPC分类号： C07K16/00 , C07K16/241 , C07K16/244 , C07K16/2854 , C12N15/79 , C12N15/85 , C12N2510/02 , C12N2830/46 , C12P21/02
摘要： Compositions and methods comprising recombinant expression vector elements (rEVEs) to enhance the level of expression of recombinant proteins are described. Other compositions and methods for lowering, substantially suppressing, or essentially silencing expression of a recombinant protein are also described.
摘要翻译：描述了包含重组表达载体元件（rEVEs）以增强重组蛋白表达水平的组合物和方法。还描述了用于降低，基本抑制或基本沉默重组蛋白表达的其他组合物和方法。

2. 发明申请

WO2007014162A3 MULTIPLE GENE EXPRESSION INCLUDING SORF CONSTRUCTS AND METHODS WITH POLYPROTEINS, PRO-PROTEINS, AND PROTEOLYSIS 审中-公开
标题翻译：多种基因表达，包括SORF构建体和方法与聚丙烯酸，蛋白质和蛋白质和蛋白质
公开(公告)号：WO2007014162A3
公开(公告)日：2009-05-07
申请号：PCT/US2006028691
申请日：2006-07-21
申请人： ABBOTT LAB , CARSON GERALD R , GION WENDY , SALFELD JOCHEN G , GU JIJIE , REGIER DEAN A , KUNES YUNE
发明人： CARSON GERALD R , GION WENDY , SALFELD JOCHEN G , GU JIJIE , REGIER DEAN A , KUNES YUNE
IPC分类号： C12N15/63 , C12P21/06 , C07H21/02 , C07H21/04 , C07K16/18 , C12N1/18 , C12N5/07 , C12N5/071 , C12N15/00 , C12P21/04 , C12P21/08
CPC分类号： C12N15/67 , C07K16/00 , C07K2319/50 , C07K2319/92 , C12N15/1055 , C12P21/02 , C12P21/06
摘要： Disclosed are useful constructs and methods for the expression of proteins using primary translation products that are processed within a recombinant host cell. Constructs comprising a single open reading frame (sORF) are described for protein expression including expression of multiple polypeptides. A primary translation product (a pro-protein or a polyprotein) contains polypeptides such as inteins or hedgehog family auto-processing domains, or variants thereof, inserted in frame between multiple protein subunits of interest. The primary product can also contain cleavage sequences such as other proteolytic cleavage or protease recognition sites, or signal peptides which contain recognition sequences for signal peptidases, separating at least two of the multiple protein subunits. The sequences of the inserted auto-processing polypeptides or cleavage sites can be manipulated to enhance the efficiency of expression of the separate multiple protein subunits. Also disclosed are independent aspects of conducting efficient expression, secretion, and/or multimeric assembly of proteins such as immunoglobulins. Where the polyprotein contains immunoglobulin heavy and light chain segments or fragments capable of antigen recognition, in an embodiment a selectable stoichiometric ratio is at least two copies of a light chain segment per heavy chain segment, with the result that the production of properly folded and assembled functional antibody is made. Modified signal peptides, including such from immunoglobulin light chains, are described.
摘要翻译：公开了使用在重组宿主细胞内处理的主要翻译产物来表达蛋白质的有用构建体和方法。描述了包含单个开放阅读框（sORF）的构建体用于蛋白质表达，包括多个多肽的表达。初级翻译产品（前蛋白质或多蛋白质）含有多肽，例如内含肽或刺猬蛋白家族自身处理结构域或其变体，其插入到感兴趣的多个蛋白质亚基之间。初级产物还可以含有裂解序列，如其他蛋白水解切割或蛋白酶识别位点，或含有信号肽酶识别序列的信号肽，分离多个蛋白质亚基中的至少两个。可以操纵插入的自动处理多肽或切割位点的序列，以提高分离的多个蛋白质亚基的表达效率。还公开了进行蛋白质例如免疫球蛋白的有效表达，分泌和/或多聚体装配的独立方面。当多蛋白含有免疫球蛋白重链和轻链片段或能够进行抗原识别的片段时，在一个实施方案中，可选择的化学计量比为每个重链段的轻链片段的至少两个拷贝，结果是适当地折叠和组装制备功能性抗体。描述了包含免疫球蛋白轻链的修饰的信号肽。

3. 发明专利

CA1340866C HUMAN C3B/C4B RECEPTOR (CR1) 未知
公开(公告)号：CA1340866C
公开(公告)日：1999-12-28
申请号：CA595389
申请日：1989-03-31
申请人： MAKRIDES SAVVAS C , WONG WINNIE W , CONCINO MICHAEL F , MARSH HENRY C JR , FEARON DOUGLAS T , IP STEPHEN H , KLICKSTEIN LLOYD B , CARSON GERALD R
发明人： MAKRIDES SAVVAS C , WONG WINNIE W , CONCINO MICHAEL F , MARSH HENRY C JR , FEARON DOUGLAS T , IP STEPHEN H , KLICKSTEIN LLOYD B , CARSON GERALD R
IPC分类号： A61K38/00 , A61K20060101 , A61P7/02 , A61P9/00 , A61P9/10 , A61P29/00 , A61P37/00 , A61P37/06 , C07H20060101 , C07H21/04 , C07K1/18 , C07K1/22 , C07K14/00 , C07K14/315 , C07K14/705 , C07K14/715 , C07K16/28 , C12N1/21 , C12N5/10 , C12N9/70 , C12N9/72 , C12N15/09 , C12N15/12 , C12P20060101 , C12P21/00 , C12P21/02 , C12R1/19 , C12R1/91 , A61K38/17 , C07K14/05 , C12N15/63
摘要： The present invention relates to the C3b/C4b receptor (CR1) gene and its encoded protein. The invention also relates to CR1 nucleic acid sequences and fragments thereof comprising 70 nucleotides and their encoded peptides or proteins comprising 24 amino acids. The invention further provides for the expression of the CR1 protein and fragments thereof. The genes and proteins of the invention have uses in diagnosis and therapy of disorders involving complement activity, and various immune system or inflammatory disorders. In specific embodiments of the present invention detailed in the examples sections infra, the cloning, nucleotide sequence, and deduced amino acid sequence of a full-length CR1 cDNA and fragments thereof are described. The expression of the CR1 protein and fragments thereof is also described. Also described is the expression of a secreted CR1 molecule lacking a transmembrane region. The secreted CR1 molecule is shown to be useful in reducing damage caused by inflammation and in reducing myocardial infarct size and preventing reperfusion injury.

4. 发明申请

WO9964560A9 METHODS FOR MONITORING THE STABILITY OF PROTEIN OR CELLULAR PRODUCT SECRETION BY CELLS IN A FERMENTER CULTURE 审中-公开
标题翻译：用于监测细胞在细胞培养物中蛋白质或细胞产物分泌稳定性的方法
公开(公告)号：WO9964560A9
公开(公告)日：2000-04-27
申请号：PCT/US9912862
申请日：1999-06-08
申请人： BASF AG , CARSON GERALD R , HAMMILL LINDA
发明人： CARSON GERALD R , HAMMILL LINDA
IPC分类号： C12M1/34 , G01N33/68 , C12N11/04
CPC分类号： G01N33/68
摘要： The invention features a method for monitoring the stability of a protein or other cellular product secreted by cells in a fermenter culture. The method involves, harvesting from a fermenter culture a first sample of cells that secrete a protein or cellular product and incorporating the cells from the first sample into gel microdrops (GMDs), and measuring secretion of the protein or cellular product by cells in one or more GMD samples over time. The protein secreted by the cells may be, for example, an antibody, cytokine, or growth factor. The cellular product secreted by the cells may be, for example, an antibiotic, amino acid, vitamin, or carbohydrate.
摘要翻译：本发明的特征在于监测发酵罐培养物中由细胞分泌的蛋白质或其它细胞产物的稳定性的方法。该方法涉及从发酵罐培养物收获分泌蛋白质或细胞产物的第一个细胞样品，并将来自第一个样品的细胞并入凝胶微滴（GMD）中，并测量细胞在一个或多个细胞中的分泌，随着时间的推移，更多的GMD样品。由细胞分泌的蛋白质可以是例如抗体，细胞因子或生长因子。由细胞分泌的细胞产物可以是例如抗生素，氨基酸，维生素或碳水化合物。

5. 发明专利

JP2012235782A Multiple gene expression including sorf construct and method with polyprotein, pro-protein, and proteolysis 审中-公开
标题翻译：多基因表达包括SORF结构和方法与聚丙烯，蛋白质和蛋白质和蛋白质
公开(公告)号：JP2012235782A
公开(公告)日：2012-12-06
申请号：JP2012148624
申请日：2012-07-02
申请人： Abbott Lab , アボット・ラボラトリーズAbbott Laboratories
发明人： CARSON GERALD R , GION WENDY , SALFELD JOCHEN G , GU JIJIE , REGIER DEAN A , KUNES YUNE
IPC分类号： C12N15/09 , C12N15/63 , C07H21/04 , C07K16/18 , C12N1/18 , C12N5/07 , C12N5/071 , C12P21/08
CPC分类号： C12N15/67 , C07K16/00 , C07K2319/50 , C07K2319/92 , C12N15/1055 , C12P21/02 , C12P21/06
摘要： PROBLEM TO BE SOLVED: To provide useful constructs and methods for the expression of proteins using primary translation products that are processed within a recombinant host cell.SOLUTION: Multiple vectors for protein expressions comprising a single open reading frame (sORF) insert are described. The expression vector includes polypeptides such as inteins or hedgehog family auto-processing domains, or variants thereof, inserted in frame between multiple protein subunits of interest contained in the primary translation product (a pro-protein or a polyprotein).
摘要翻译：要解决的问题：提供用于使用在重组宿主细胞内处理的初级翻译产物来表达蛋白质的有用构建体和方法。描述了包含单个开放阅读框（sORF）插入物的蛋白质表达的多个载体。表达载体包括插入到主要翻译产物（前蛋白质或多蛋白）中包含的感兴趣的多个蛋白质亚基之间的多肽，例如内含肽或刺猬蛋白家族自身处理结构域或其变体。版权所有（C）2013，JPO＆INPIT

6. 发明公开

EP1910550A4 MULTIPLE GENE EXPRESSION INCLUDING SORF CONSTRUCTS AND METHODS WITH POLYPROTEINS, PRO-PROTEINS, AND PROTEOLYSIS 审中-公开
标题翻译：表达的几个基因，OF SORF构建体及与多聚PROPROTEINEN和蛋白水解方法，包括
公开(公告)号：EP1910550A4
公开(公告)日：2009-11-04
申请号：EP06788320
申请日：2006-07-21
申请人： ABBOTT LAB
发明人： CARSON GERALD R , GION WENDY , SALFELD JOCHEN G , GU JIJIE , REGIER DEAN A , KUNES YUNE
IPC分类号： C12N15/63 , C12P21/06 , C07H21/02 , C07H21/04 , C07K16/18 , C12N1/18 , C12N5/07 , C12N5/071 , C12N15/00 , C12P21/08
CPC分类号： C12N15/67 , C07K16/00 , C07K2319/50 , C07K2319/92 , C12N15/1055 , C12P21/02 , C12P21/06

7. 发明公开

EP2142560A4 RECOMBINANT EXPRESSION VECTOR ELEMENTS (REVES) FOR ENHANCING EXPRESSION OF RECOMBINANT PROTEINS IN HOST CELLS 审中-公开
标题翻译：重组表达载体ELEMENTS（雷沃）改善重组蛋白在宿主细胞中表达
公开(公告)号：EP2142560A4
公开(公告)日：2010-12-01
申请号：EP08727191
申请日：2008-03-28
申请人： ABBOTT LAB
发明人： GION WENDY R , CARSON GERALD R , GAO HONG , KUNES YUNE Z
IPC分类号： C12N15/85
CPC分类号： C07K16/00 , C07K16/241 , C07K16/244 , C07K16/2854 , C12N15/79 , C12N15/85 , C12N2510/02 , C12N2830/46 , C12P21/02

8. 发明专利

IN3281DEN2012A "SORF CONSTRUCTS AND MULTIPLE GENE EXPRESSION" 未知
公开(公告)号：IN3281DEN2012A
公开(公告)日：2015-10-23
申请号：IN3281DEN2012
申请日：2012-04-16
申请人： ABBOTT LAB
发明人： CARSON GERALD R , GION WENDY R , KUNES YUNE Z , LEISE III WALTER F , DAVIS-TABER RACHEL A , FUNG EMMA
IPC分类号： C07K16/00
摘要： Embodiments of the invention relate to vector constructs and methods for expression of polypeptides including multimeric products such as therapeutic antibodies. Particular constructs allow for the generation of expression products from a single open reading frame (sORF). An embodiment provides an isolated or purified expression vector for generating one or more recombinant protein products comprising a single open reading frame insert; said insert comprising a signal peptide nucleic acid sequence encoding a signal peptide; a first nucleic acid sequence encoding a first polypeptide; a first intervening nucleic acid sequence encoding a first protein cleavage site, wherein said first protein cleavage site is provided by an intein segment of a Ion protease gene of Pyrococcus or a klbA gene of Pyrococcus or Methanococcus, or a modified intein segment derived therefrom; and a second nucleic acid sequence encoding a second polypeptide. Certain embodiments of constructs and methods employ an intein segment of a Ion protease gene of Pyrococcus abyssi, Pyrococcus furiosus, or Pyrococcus horikoshii OT3; or an intein segment of a klbA gene of Pyrococcus abyssi, Pyrococcus furiosus, or Methanococcus jannaschir, or other intein segment.

9. 发明专利

MX2008000985A EXPRESION DE GEN MULTIPLE QUE INCLUYE CONSTRUCCIONES Y METODOS SORF (CUADRO DE LECTURA ABIERTA SIMPLE) CON POLIPROTEINAS, PRO-PROTEINAS Y PROTEOLISIS. 未知
公开(公告)号：MX2008000985A
公开(公告)日：2008-04-07
申请号：MX2008000985
申请日：2006-07-21
申请人： ABBOTT LAB
发明人： CARSON GERALD R , GION WENDY , SALFELD JOCHEN G , GU JIJIE , REGIER DEAN A , KUNES YUNE
IPC分类号： C12N15/63 , C12P21/08 , C07H21/04 , C07K16/18 , C12N1/18 , C12N5/07 , C12N5/071
摘要： Se describen construcciones y metodos utiles para la expresion de proteinas utilizando productos de traduccion primaria que son procesados dentro de una celula huesped recombinante. Las construcciones que comprenden una sola estructura de lectura abierta (sORF) se describen para la expresion de proteinas incluyendo expresion de polipeptidos multiples. Un producto de traduccion primaria (una proteina o poliproteina) contiene polipeptidos tales como inteinas o dominios de auto-procesamiento de la familia de erizos, o variantes de los mismos, insertadas en la estructura entre multiples subunidades de proteina de interes. El producto primario tambien puede contener secuencias de disociacion, tales como otros sitios de reconocimiento de proteasa o disociacion proteolitica, o peptidos de senal que contienen secuencias de reconocimiento para peptidasas de senal, que separa al menos dos de las multiples subunidades de proteina. Las secuencias de los polipeptidos de auto-procesamiento o sitios de disociacion insertados, pueden manipularse para mejorar la eficiencia de expresion de las subunidades de proteina multiple separadas. Tambien se describen aspectos independientes para llevar a cabo una expresion, secrecion, y/o ensamble de proteinas multimerico eficiente, tal como inmunoglobulinas. Cuando la poliproteina contiene segmentos de cadena pesada y ligera de inmunoglobulina o fragmentos con la capacidad de reconocimiento de antigenos, en una modalidad una proporcion estequiometrica seleccionable es de al menos dos copias de un segmento de cadena ligera por segmento de cadena pesada, obteniendo como resultado que se lleve a cabo la produccion del anticuerpo funcional doblado y ensamblado. Se describen peptidos de senal modificados, incluyendo los que proceden de cadenas ligeras de inmunoglobulina.

10. 发明专利

CA2615983A1 MULTIPLE GENE EXPRESSION INCLUDING SORF CONSTRUCTS AND METHODS WITH POLYPROTEINS, PRO-PROTEINS, AND PROTEOLYSIS 未知
公开(公告)号：CA2615983A1
公开(公告)日：2007-02-01
申请号：CA2615983
申请日：2006-07-21
申请人： ABBOTT LAB
发明人： GU JIJIE , SALFELD JOCHEN G , REGIER DEAN A , KUNES YUNE , GION WENDY , CARSON GERALD R
IPC分类号： C12N15/63 , C12P21/08 , C07H21/04 , C07K16/18 , C12N1/18 , C12N5/07 , C12N5/071
摘要： Disclosed are useful constructs and methods for the expression of proteins using primary translation products that are processed within a recombinant host cell. Constructs comprising a single open reading frame (sORF) are described for protein expression including expression of multiple polypeptides. A primary translation product (a pro-protein or a polyprotein) contains polypeptides such as inteins or hedgehog family auto-processing domains, or variants thereof, inserted in frame between multiple protein subunits of interest. The primary product can also contain cleavage sequences such as other proteolytic cleavage or protease recognition sites, or signal peptides which contain recognition sequences for signal peptidases, separating at least two of the multiple protein subunits. The sequences of the inserted auto-processing polypeptides or cleavage sites can be manipulated to enhance the efficiency of expression of the separate multiple protein subunits. Also disclosed are independent aspects of conducting efficient expression, secretion, and/or multimeric assembly of proteins such as immunoglobulins. Where the polyprotein contains immunoglobulin heavy and light chain segments or fragments capable of antigen recognition, in an embodiment a selectable stoichiometric ratio is at least two copies of a light chain segment per heavy chain segment, with the result that the production of properly folded and assembled functional antibody is made. Modified signal peptides, including such from immunoglobulin light chains, are described.

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