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1. 发明申请

WO2008121324A2 RECOMBINANT EXPRESSION VECTOR ELEMENTS (REVES) FOR ENHANCING EXPRESSION OF RECOMBINANT PROTEINS IN HOST CELLS 审中-公开
标题翻译：用于增强宿主细胞中重组蛋白表达的重组表达载体元件（REVES）
公开(公告)号：WO2008121324A2
公开(公告)日：2008-10-09
申请号：PCT/US2008/004063
申请日：2008-03-28
申请人： ABBOTT LABORATORIES , GION, Wendy, R. , CARSON, Gerald, R. , GAO, Hong , KUNES, Yune, Z.
发明人： GION, Wendy, R. , CARSON, Gerald, R. , GAO, Hong , KUNES, Yune, Z.
IPC分类号： C12N15/00 , C12N5/00 , C12N5/02
CPC分类号： C07K16/00 , C07K16/241 , C07K16/244 , C07K16/2854 , C12N15/79 , C12N15/85 , C12N2510/02 , C12N2830/46 , C12P21/02
摘要： Compositions and methods comprising recombinant expression vector elements (rEVEs) to enhance the level of expression of recombinant proteins are described. Other compositions and methods for lowering, substantially suppressing, or essentially silencing expression of a recombinant protein are also described.
摘要翻译：描述了包含重组表达载体元件（rEVE）以增强重组蛋白质表达水平的组合物和方法。还描述了用于降低，基本抑制或基本上沉默重组蛋白的表达的其它组合物和方法。

2. 发明申请

WO2011053699A1 SORF CONSTRUCTS AND MULTIPLE GENE EXPRESSION 审中-公开
标题翻译： SORF构造和多基因表达
公开(公告)号：WO2011053699A1
公开(公告)日：2011-05-05
申请号：PCT/US2010/054475
申请日：2010-10-28
申请人： ABBOTT LABORATORIES , CARSON, Gerald R. , GION, Wendy R. , KUNES, Yune Z. , LEISE III, Walter F. , DAVIS-TABER, Rachel A. , FUNG, Emma
发明人： CARSON, Gerald R. , GION, Wendy R. , KUNES, Yune Z. , LEISE III, Walter F. , DAVIS-TABER, Rachel A. , FUNG, Emma
IPC分类号： C07K16/00 , C12P21/02 , C12P21/06
CPC分类号： C07K16/00 , C07K16/241 , C07K16/244 , C07K2317/14 , C07K2317/21 , C07K2317/76 , C07K2319/50 , C07K2319/92
摘要： Embodiments of the invention relate to vector constructs and methods for expression of polypeptides including multimeric products such as therapeutic antibodies. Particular constructs allow for the generation of expression products from a single open reading frame (sORF). An embodiment provides an isolated or purified expression vector for generating one or more recombinant protein products comprising a single open reading frame insert; said insert comprising a signal peptide nucleic acid sequence encoding a signal peptide; a first nucleic acid sequence encoding a first polypeptide; a first intervening nucleic acid sequence encoding a first protein cleavage site, wherein said first protein cleavage site is provided by an intein segment of a Ion protease gene of Pyrococcus or a klbA gene of Pyrococcus or Methanococcus , or a modified intein segment derived therefrom; and a second nucleic acid sequence encoding a second polypeptide. Certain embodiments of constructs and methods employ an intein segment of a Ion protease gene of Pyrococcus abyssi , Pyrococcus furiosus , or Pyrococcus horikoshii OT3; or an intein segment of a klbA gene of Pyrococcus abyssi , Pyrococcus furiosus , or Methanococcus jannaschii ; or other intein segment.
摘要翻译：本发明的实施方案涉及用于表达多肽的载体构建体和方法，包括多聚体产物，例如治疗性抗体。特定结构允许从单个开放阅读框（sORF）生成表达产物。一个实施方案提供了用于产生一种或多种包含单个开放阅读框插入物的重组蛋白产物的分离或纯化的表达载体; 所述插入片段包含编码信号肽的信号肽核酸序列; 编码第一多肽的第一核酸序列; 编码第一蛋白切割位点的第一插入核酸序列，其中所述第一蛋白切割位点由Pyrococcus的离子蛋白酶基因的内含肽片段或Pyrococcus或Methanococcus的klbA基因或由其衍生的修饰的内含肽片段提供; 和编码第二多肽的第二核酸序列。构建体和方法的某些实施方案采用激烈的球孢球菌，激烈火球菌或火球菌OT3的离子蛋白酶基因的内含肽片段; 或激烈球菌（Crrococcus abyssi），激烈火球菌（Pyrococcus furiosus）或季戊糖（Methanococcus jannaschii）的klbA基因的内含肽片段; 或其他内含肽片段。

3. 发明申请

WO2008121324A3 RECOMBINANT EXPRESSION VECTOR ELEMENTS (REVES) FOR ENHANCING EXPRESSION OF RECOMBINANT PROTEINS IN HOST CELLS 审中-公开
标题翻译：重组表达载体元件（改良）增强重组蛋白在宿主细胞中的表达
公开(公告)号：WO2008121324A3
公开(公告)日：2008-12-11
申请号：PCT/US2008004063
申请日：2008-03-28
申请人： ABBOTT LAB , GION WENDY R , CARSON GERALD R , GAO HONG , KUNES YUNE Z
发明人： GION WENDY R , CARSON GERALD R , GAO HONG , KUNES YUNE Z
IPC分类号： C07H21/04
CPC分类号： C07K16/00 , C07K16/241 , C07K16/244 , C07K16/2854 , C12N15/79 , C12N15/85 , C12N2510/02 , C12N2830/46 , C12P21/02
摘要： Compositions and methods comprising recombinant expression vector elements (rEVEs) to enhance the level of expression of recombinant proteins are described. Other compositions and methods for lowering, substantially suppressing, or essentially silencing expression of a recombinant protein are also described.
摘要翻译：描述了包含重组表达载体元件（rEVEs）以增强重组蛋白表达水平的组合物和方法。还描述了用于降低，基本抑制或基本沉默重组蛋白表达的其他组合物和方法。

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