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    • 6. 发明公开
    • 지방유래 줄기세포를 이용한 성장인자의 대량 생산방법
    • 使用ADIPOSE衍生成熟干细胞的生长因子的质量生产方法
    • KR1020080109725A
    • 2008-12-17
    • KR1020087019093
    • 2006-10-12
    • 주프로스테믹스박병순
    • 박병순최봉근박철홍
    • C12N5/071C12N5/07C12N5/02
    • C07K14/503C07K14/495C07K14/52C12N5/0667C12N2500/02C12N2500/38C12N2500/60C12N2500/90C12N2500/99C12N2502/1382C12N2510/02
    • A mass-production method of the growth factor by using the adipose derived stem cell is provided to improve the in vivo activity compared to the growth factor synthesized with recombinant or chemical method. The method for massively producing the human growth factor from the adipose derived stem cell comprises the steps of: (a) isolating the adipose-derived adult stem cell extracted from the adipocyte of mammals; (b) selectively culturing the stem cell in the serum medium, and subculturing it in the serum free medium; (c) adding one or more physical stimuli selected from the group consisting of low oxygen concentration cultivation, ultraviolet irradiation, nutrient deficiency and mechanical friction to the adipose derived adult stem cell; and (d) selectively adding one or more vitamins selected from vitamin A, vitamin B, vitamin C and vitamin D into the culture medium, provided that the steps (c) and (d) are performed through the combinations under the condition to occur the maximum production of human growth factor.
    • 提供通过使用脂肪来源的干细胞的生长因子的批量生产方法,以提高与用重组或化学方法合成的生长因子相比的体内活性。 从脂肪来源的干细胞大量生产人生长因子的方法包括以下步骤:(a)分离从哺乳动物的脂肪细胞提取的脂肪来源的成体干细胞; (b)选择性培养血清培养基中的干细胞,并将其传代培养在无血清培养基中; (c)向脂肪来源的成体干细胞中加入选自低氧浓度培养,紫外线照射,营养缺乏和机械摩擦的一种或多种物理刺激物; 和(d)选择性地将一种或多种选自维生素A,维生素B,维生素C和维生素D的维生素添加到培养基中,条件是步骤(c)和(d)通过组合在条件下进行 最大生产人体生长因子。
    • 8. 发明公开
    • 지방 줄기세포의 조직 재생 능력을 최적화한 조건 배지를 함유한 조직 재생용 주사제 첨가제
    • 用于组织修复的注射剂的组合物,包括ADIPOSE衍生的干细胞和优化的培养基
    • KR1020080075959A
    • 2008-08-20
    • KR1020070015211
    • 2007-02-14
    • 주프로스테믹스박병순
    • 박병순박정수
    • C12N5/0775
    • C12N5/0667A61K35/12A61K2035/124C12N2500/32C12N2500/38C12N2500/90C12N2501/115C12N2501/12C12N2501/135C12N2501/15C12N2501/165C12N2501/385C12N2501/58C12N2501/998C12N2501/10
    • An injection composition comprising culture medium for optimizing efficacy of human adipose stem cells is provided to increase tissue engraftment efficiency of adipose stem cells, improve differentiation ability of fibroblasts and enhance tissue repair ability as compared to single injection of adipose stem cells. An injection composition for tissue repair comprises cultured medium of human adipose stem cells which is prepared by culturing human adipose stem cells in serum free medium containing adipose stem cell-activating compounds including antioxidant, vitamins or their derivatives, amino acids or their derivatives, inorganic matters, and optionally growth factor and collagen for more than 1 day. The skin tissue is repaired by injecting the composition for tissue repair together with the adipose stem cell into the skin tissue. Further, the amino acids or their derivatives are one or more selected from L-Alanine, L-Asparitic acid, L-Histdine, L-leucine or L- glutamine.
    • 提供了包含用于优化人类脂肪干细胞功效的培养基的注射组合物,以增加脂肪干细胞的组织移植效率,与单次注射脂肪干细胞相比,提高成纤维细胞的分化能力和增强组织修复能力。 用于组织修复的注射组合物包括通过在包含抗氧化剂,维生素或其衍生物,氨基酸或其衍生物的脂肪干细胞活化化合物的无血清培养基中培养人类脂肪干细胞而制备的人类脂肪干细胞的培养基,无机物 ,以及任选的生长因子和胶原蛋白超过1天。 通过将用于组织修复的组合物与脂肪干细胞一起注入皮肤组织来修复皮肤组织。 此外,氨基酸或其衍生物是选自L-丙氨酸,L-天冬氨酸,L-丝氨酸,L-亮氨酸或L-谷氨酰胺中的一种或多种。
    • 9. 发明授权
    • 줄기세포 배양액을 이용한 멜라닌 합성저해 방법
    • 使用成熟干细胞培养的梅毒素合成的抑制
    • KR100848056B1
    • 2008-07-23
    • KR1020070076221
    • 2007-07-30
    • 주프로스테믹스박병순
    • 박병순김원석성종혁안세진
    • A61K8/64A61K8/98A61Q19/02
    • A61Q19/02A61K8/99A61K35/28A61K35/51
    • A cosmetic composition comprising the cultured medium of adult stem cells is provided to inhibit synthesis of melanin and activity of tyrosinase without side effects, so that the composition is useful for whitening the skin. A cosmetic composition for whitening the skin comprises the cultured medium of adult stem cells derived from mammals, especially adipocytes, bone marrow tissue and umbilical cord blood of mammals or proteins separated from the cultured medium, wherein the adult stem cell is isolated from adipocytes by obtaining fat tissue through liposuction, treating the extracellular matrix of fat tissue with collagenase, centrifuging the enzyme-treated product to divide the enzyme-treated product into monocytes, hematocytes and several cell debris.
    • 提供包含成体干细胞的培养基的化妆品组合物,以抑制黑色素的合成和酪氨酸酶的活性而没有副作用,使得该组合物可用于使皮肤美白。 用于使皮肤美白的化妆品组合物包含哺乳动物,特别是脂肪细胞,骨髓组织和来自培养基的蛋白质分离的骨髓组织和脐带血的成体干细胞的培养基,其中通过获得成体干细胞从脂肪细胞分离 通过脂肪抽吸脂肪组织,用胶原酶处理脂肪组织的细胞外基质,离心酶处理产物以将酶处理的产物分成单核细胞,血细胞和几个细胞碎片。