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1. 发明公开

KR1020100036389A 지방유래 줄기세포 배양액을 함유하는 화장료 조성물 无效
标题翻译：包含ADIPOSE衍生干细胞的调节媒质的化妆品组合物
公开(公告)号：KR1020100036389A
公开(公告)日：2010-04-07
申请号：KR1020107005233
申请日：2006-10-12
申请人： 주프로스테믹스 , 박병순
发明人： 박병순 , 최봉근 , 박철홍
IPC分类号： A61K8/98 , C12N5/0775 , C12N5/02 , C12N13/00
CPC分类号： C07K14/503 , C07K14/495 , C07K14/52 , C12N5/0667 , C12N2500/02 , C12N2500/38 , C12N2500/60 , C12N2500/90 , C12N2500/99 , C12N2502/1382 , C12N2510/02
摘要： PURPOSE: A functional cosmetic composition containing human adipose-derived stem cell culture media is provided to effectively prevent wrinkle and aging without side effects. CONSTITUTION: A method for producing stem cell culture medium comprises: a step of separating adipose-derived adult stem cells extracted from adipocytes of mammal; a step of sub-culturing the stem cells serum medium and then culturing serum-free medium; a step of performing hypoxia culture, UV irradiation, nutrition deficiency and mechanical rubbing of adipose-derived stem cells; and a step of adding vitamin A, B, C or D to the culture media.
摘要翻译：目的：提供含有人脂肪干细胞培养基的功能性化妆品组合物，以有效防止皱纹和老化而无副作用。构成：用于生产干细胞培养基的方法包括：分离从哺乳动物脂肪细胞提取的脂肪来源的成体干细胞的步骤; 培养干细胞血清培养基，然后培养无血清培养基的步骤; 进行缺氧培养，UV照射，营养缺乏和脂肪干细胞的机械摩擦的步骤; 以及向培养基中加入维生素A，B，C或D的步骤。

2. 发明授权

KR100955212B1 지방유래 줄기세포를 이용한 성장인자의 대량 생산방법 有权
标题翻译：使用ADIPOSE衍生成熟干细胞的生长因子的质量生产方法
公开(公告)号：KR100955212B1
公开(公告)日：2010-04-29
申请号：KR1020087019093
申请日：2006-10-12
申请人： 주프로스테믹스 , 박병순
发明人： 박병순 , 최봉근 , 박철홍
IPC分类号： C12N5/071 , C12N5/07 , C12N5/02
CPC分类号： C07K14/503 , C07K14/495 , C07K14/52 , C12N5/0667 , C12N2500/02 , C12N2500/38 , C12N2500/60 , C12N2500/90 , C12N2500/99 , C12N2502/1382 , C12N2510/02
摘要： 본 발명은 인간의 지방유래 줄기세포를 이용하여 인간의 성장인자를 대량으로 생산하는 방법에 관한 것으로서, 구체적으로 본 발명은 인간의 지방세포에서 추출된 지방유래 줄기세포를 적정한 배지 및 조건에서 배양하여 인간 성장인자를 현저히 많은 양으로 합성할 수 있도록 조작하는 방법을 제공한다. 또한 본 발명의 방법에 따라 생산된 줄기세포 배양액 및 그로부터 분리한 인간 성장인자는 약품 및 화장품의 원료로 유용하게 이용될 수 있다.

3. 发明公开

KR1020080109725A 지방유래 줄기세포를 이용한 성장인자의 대량 생산방법 有权
标题翻译：使用ADIPOSE衍生成熟干细胞的生长因子的质量生产方法
公开(公告)号：KR1020080109725A
公开(公告)日：2008-12-17
申请号：KR1020087019093
申请日：2006-10-12
申请人： 주프로스테믹스 , 박병순
发明人： 박병순 , 최봉근 , 박철홍
IPC分类号： C12N5/071 , C12N5/07 , C12N5/02
CPC分类号： C07K14/503 , C07K14/495 , C07K14/52 , C12N5/0667 , C12N2500/02 , C12N2500/38 , C12N2500/60 , C12N2500/90 , C12N2500/99 , C12N2502/1382 , C12N2510/02
摘要： A mass-production method of the growth factor by using the adipose derived stem cell is provided to improve the in vivo activity compared to the growth factor synthesized with recombinant or chemical method. The method for massively producing the human growth factor from the adipose derived stem cell comprises the steps of: (a) isolating the adipose-derived adult stem cell extracted from the adipocyte of mammals; (b) selectively culturing the stem cell in the serum medium, and subculturing it in the serum free medium; (c) adding one or more physical stimuli selected from the group consisting of low oxygen concentration cultivation, ultraviolet irradiation, nutrient deficiency and mechanical friction to the adipose derived adult stem cell; and (d) selectively adding one or more vitamins selected from vitamin A, vitamin B, vitamin C and vitamin D into the culture medium, provided that the steps (c) and (d) are performed through the combinations under the condition to occur the maximum production of human growth factor.
摘要翻译：提供通过使用脂肪来源的干细胞的生长因子的批量生产方法，以提高与用重组或化学方法合成的生长因子相比的体内活性。从脂肪来源的干细胞大量生产人生长因子的方法包括以下步骤：（a）分离从哺乳动物的脂肪细胞提取的脂肪来源的成体干细胞; （b）选择性培养血清培养基中的干细胞，并将其传代培养在无血清培养基中; （c）向脂肪来源的成体干细胞中加入选自低氧浓度培养，紫外线照射，营养缺乏和机械摩擦的一种或多种物理刺激物; 和（d）选择性地将一种或多种选自维生素A，维生素B，维生素C和维生素D的维生素添加到培养基中，条件是步骤（c）和（d）通过组合在条件下进行最大生产人体生长因子。

4. 发明公开

KR1020070000005A 섬유아세포성장인자를 함유한 화장료 조성물 无效
标题翻译：含有纤维素生长因子的化妆品组合物
公开(公告)号：KR1020070000005A
公开(公告)日：2007-01-02
申请号：KR1020050055017
申请日：2005-06-24
申请人： 주프로스테믹스
发明人： 최봉근 , 박병순 , 박철홍
IPC分类号： A61K8/64 , A61Q19/08
CPC分类号： A61K8/64 , A61K8/345 , A61K8/35 , A61K8/72 , A61K35/28 , A61K38/1825 , A61Q19/08
摘要： A cosmetic composition containing a fibroblast growth factor is provided to improve skin wrinkles, enhance safety of fibroblast growth factor by separating it from adipocytes, and avoid titer lowering of fibroblast growth factor by stabilizing it with hydrophilic polymer resin. The cosmetic composition for improving wrinkles and inhibiting skin aging contains the fibroblast growth factor, wherein the amount of the fibroblast growth factor is 0.00000001 to 0.001%(w/v); the composition further contains one component selected from hydrophilic polymer resin, multivalent alcohol and UV screen; the hydrophilic polymer resin is acrylate copolymer, allyl acrylate copolymer or alkyl acrylate copolymer and has molecular weight of 1,000 to 1,000,000 dalton; the multivalent alcohol is glycerol, butyleneglycol, propyleneglycol or polyoxyethylene; the pH of the composition is 6-9; and the composition is formulated as nutrition cream, massage cream, essence or pack.
摘要翻译：提供含有成纤维细胞生长因子的化妆品组合物以改善皮肤皱纹，通过将其与脂肪细胞分离来增强成纤维细胞生长因子的安全性，并通过用亲水性聚合物树脂稳定来避免成纤维细胞生长因子的滴度降低。用于改善皱纹和抑制皮肤老化的化妆品组合物含有成纤维细胞生长因子，其中成纤维细胞生长因子的量为0.00000001至0.001％（w / v）; 该组合物还含有选自亲水性聚合物树脂，多价醇和UV屏幕的一种组分; 亲水性聚合物树脂是丙烯酸酯共聚物，丙烯酸烯丙酯共聚物或丙烯酸烷基酯共聚物，分子量为1,000至1,000,000道尔顿; 多元醇是甘油，丁二醇，丙二醇或聚氧乙烯; 组合物的pH为6-9; 并将该组合物配制成营养霜，按摩膏，精华液或包装。

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