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    • 3. 发明专利
    • Method for regulating enzymatic reaction and device of regulation
    • 用于调节酶促反应的方法和调节装置
    • JP2007089541A
    • 2007-04-12
    • JP2005287056
    • 2005-09-30
    • Ehime PrefectureEhime UnivNational Institute Of Advanced Industrial & Technology国立大学法人愛媛大学愛媛県独立行政法人産業技術総合研究所
    • SHINTANI TOMOKICHIHIGAKI SEIJIHAGABE REITORIMURA MASAMOTOMANABE TAKASHI
    • C12P1/00C12M1/42
    • PROBLEM TO BE SOLVED: To provide (1) a method and device for regulating an enzymatic reaction useful for an efficient enzymatic reaction, and also (2) a device and method for separating reaction products produced by the enzymatic reaction from a substrate at the same time with the enzymatic reaction and obtaining the reaction products by separating them to a product having a positive charge and product having a negative charge. SOLUTION: (1) This enzymatic reaction of a hydrolase is regulated by impressing an electric voltage on a reaction solution containing the hydrolase and its substrate. (2) By impressing the electric voltage on the reaction solution containing the enzyme and substrate in the reaction vessel, the enzymatic reaction products are produced in the reaction solution. By transporting the reaction products having the positive charge and negative charge among the enzymatic reaction products to each of the cathode side and anode side of an electric field formed in the reaction solution, and further by transferring the reaction products having the positive charge and negative charge to each of a separating vessel (-) and separating vessel (+), each communicating with the cathode side and anode side of the reaction vessel through ultra-filtration membranes, the enzymatic reaction products are produced and separated. COPYRIGHT: (C)2007,JPO&INPIT
    • 待解决的问题:提供(1)用于调节有效的酶促反应的酶反应的方法和装置,以及(2)将由酶反应产生的反应产物从底物分离的装置和方法 同时进行酶反应,并通过将反应产物分离成具有正电荷的产物和具有负电荷的产物来获得反应产物。 解决方案:(1)通过在含有水解酶及其底物的反应溶液上施加电压来调节水解酶的这种酶促反应。 (2)通过在反应容器中对包含酶和底物的反应溶液施加电压,在反应溶液中产生酶反应产物。 通过在酶反应产物中将具有正电荷和负电荷的反应产物输送到形成在反应溶液中的电场的阴极侧和阳极侧,并且通过转移具有正电荷和负电荷的反应产物 分离容器( - )和分离容器(+),每个分离容器(+)通过超滤膜与反应容器的阴极侧和阳极侧连通,产生和分离酶反应产物。 版权所有(C)2007,JPO&INPIT
    • 7. 发明专利
    • Method for producing microorganism-fermented product
    • 生产微生物发酵产品的方法
    • JP2011135863A
    • 2011-07-14
    • JP2010170616
    • 2010-07-29
    • Ehime PrefectureJapan Environment Planning Co LtdSekisui Chem Co Ltd愛媛県日本環境設計株式会社積水化学工業株式会社
    • NAKAMURA KENJIHIGAKI SEIJIYATSUKA AIMITSUCHIYAMA KAZUOIWANE KAZUYOSHITAKAO MASAKI
    • C12P7/08
    • PROBLEM TO BE SOLVED: To provide a method for producing a microorganism-fermented product by which the yield of the microorganism-fermented product obtained from one batch of a reaction liquid can be improved by improving the reaction velocity for enzymatically hydrolyzing cellulose contained in a cellulose-containing material by pretreatment, and improving the conversion ratio of the cellulose to saccharides in the one batch of the reaction liquid. SOLUTION: The method for producing the microorganism-fermented product includes carrying out in order the alkali treatment of the cellulose-containing material, the cleaning of the alkali-treated cellulose-containing material with water or an acidic aqueous solution, the first enzyme treatment of bringing the cleaned cellulose-containing material into contact with an aqueous solution containing a cellulolytic enzyme at an enzyme activation temperature of the cellulolytic enzyme, the first fermentation treatment of bringing the obtained enzyme-treated reaction liquid into contact with the fermentation microorganisms at a fermentation activation temperature, the second enzyme treatment of regulating the obtained reaction liquid of the fermentation treatment at the enzyme activation temperature, and the second fermentation treatment of regulating the obtained enzyme-treated reaction liquid at the fermentation activation temperature. COPYRIGHT: (C)2011,JPO&INPIT
    • 待解决的问题:提供一种生产微生物发酵产物的方法,通过该方法可以通过提高酶水解所含的纤维素的反应速度来提高由一批反应液获得的微生物发酵产物的产率 在通过预处理的含纤维素材料中,提高一批反应液中的纤维素与糖的转化率。 解决方案:微生物发酵产品的制备方法包括依次进行含纤维素材料的碱处理,用水或酸性水溶液清洗碱处理的含纤维素材料,第一 在纤维素分解酶的酶活化温度下使经清洗的含纤维素材料与含有纤维素分解酶的水溶液接触的酶处理,使得到的经酶处理的反应液体与发酵微生物接触的第一发酵处理 发酵活化温度,第二酶处理在酶活化温度下调节所得反应液的发酵处理,第二发酵处理在发酵活化温度下调节所得酶处理反应液。 版权所有(C)2011,JPO&INPIT