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1. 发明授权

US06919195B2 Glutaminase, glutaminase gene, novel recombinant DNA, and process for manufacturing glutaminase 有权
标题翻译：谷氨酰胺酶，谷氨酰胺酶基因，新型重组DNA和制备谷氨酰胺酶的方法
公开(公告)号：US06919195B2
公开(公告)日：2005-07-19
申请号：US10175002
申请日：2002-06-20
申请人： Kenichiro Matsushima , Kotaro Ito , Yasuji Koyama
发明人： Kenichiro Matsushima , Kotaro Ito , Yasuji Koyama
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/80 , C12N9/78 , C12N15/55 , C12P13/14
CPC分类号： C12N9/80
摘要： Glutaminase which comprises (a) a protein comprising an amino acid sequence shown in SEQ ID NO: 2, or (b) a protein which comprises an amino acid sequence having deletion, substitution, or addition of one or plurality of amino acids relative to the amino acid sequence (a), and has glutaminase activity. A glutaminase gene which encodes (a) a protein comprising an amino acid sequence shown in SEQ ID NO: 2, or (b) a protein which comprises an amino acid sequence having deletion, substitution, or addition of one or a plurality of amino acids relative to the amino acid sequence (a), and has glutaminase activity. The present invention enables glutaminase to be produced efficiently and thus greatly contributes to the relevant industries.
摘要翻译：谷氨酰胺酶，其包含（a）包含SEQ ID NO：2所示的氨基酸序列的蛋白质，或（b）蛋白质，其包含相对于所述氨基酸序列具有缺失，取代或添加一个或多个氨基酸的氨基酸序列氨基酸序列（a），并具有谷氨酰胺酶活性。编码（a）包含SEQ ID NO：2所示的氨基酸序列的蛋白质的谷氨酰胺酶基因，或（b）包含具有缺失，取代或添加一个或多个氨基酸的氨基酸序列的蛋白质相对于氨基酸序列（a），具有谷氨酰胺酶活性。本发明能够有效地生产谷氨酰胺酶，从而大大有助于相关行业。

2. 发明授权

US06706492B2 Multiply transformed koji mold and a method of manufacturing a flavor enhancer using the same 失效
标题翻译：倍增转化的曲霉菌和使用其制造风味增强剂的方法
公开(公告)号：US06706492B2
公开(公告)日：2004-03-16
申请号：US09801734
申请日：2001-03-09
申请人： Genryou Umitsuki , Hiroe Sato , Misao Sugishita , Yaichi Fukushima , Yasuji Koyama
发明人： Genryou Umitsuki , Hiroe Sato , Misao Sugishita , Yaichi Fukushima , Yasuji Koyama
IPC分类号： C12N115
CPC分类号： C12N1/14 , A23J3/346 , A23L27/50 , C12N9/62
摘要： The present invention provides a koji mold having increased protease activity and peptidase activity relative to a parent strain, a method of breeding the koji mold, and a method of manufacturing a flavor enhancer using the koji mold. More specifically, the present invention provides (1) a koji mold having increased protease activity and peptidase activity relative to a parent strain obtained by transformation using a protease gene and a peptidase gene, (2) a method of breeding the above koji mold which comprises transforming a parent strain of koji mold using a protease gene and a peptidase gene, and then selecting a transformant having higher protease activity and peptidase activity relative to a parent strain, and (3) a method of manufacturing a flavor enhancer which comprises allowing a culture product of the above koji mold to act on a protein.
摘要翻译：本发明提供了相对于亲本菌株具有增加的蛋白酶活性和肽酶活性的曲霉菌，使用该曲霉菌制造风味增强剂的方法。更具体地，本发明提供了（1）相对于通过使用蛋白酶基因和肽酶基因转化而获得的亲本菌株，具有增加的蛋白酶活性和肽酶活性的曲霉，（2）育种上述曲霉菌的方法，其包含使用蛋白酶基因和肽酶基因转化真菌的母本菌株，然后选择相对于亲本菌株具有较高蛋白酶活性和肽酶活性的转化体，和（3）制备风味增强剂的方法，其包括使培养物上述曲霉菌的产物作用于蛋白质。

3. 发明授权

US06541236B2 Protein having glutaminase activity and gene encoding the same 失效
公开(公告)号：US06541236B2
公开(公告)日：2003-04-01
申请号：US09946678
申请日：2001-09-06
申请人： Kotaro Ito , Genryou Umitsuki , Yasuji Koyama
发明人： Kotaro Ito , Genryou Umitsuki , Yasuji Koyama
IPC分类号： C12N978
CPC分类号： C12N9/80 , C12Y305/01002
摘要： There are disclosed a protein having an amino acid sequence represented by amino acid numbers 1 to 684 or 49 to 684 shown in SEQ ID NO:2, or a protein having a glutaminase activity in which one or more amino acids is/are deleted from, substituted by, inserted to or added to the amino acid sequence of the above protein; a gene containing DNA encoding the above protein or a gene encoding a protein which hybridizes with the DNA of the above gene under a stringent condition and has a glutaminase activity; a recombinant DNA containing the above gene; a transformant or a transductant containing the above recombinant DNA; and a process for producing glutaminase which comprises culturing the above transformant or the above transductant and collecting glutaminase from a culture medium.

4. 发明授权

US06228626B1 Sarcosine oxidase and process for producing the same 失效
标题翻译：肌氨酸氧化酶及其制备方法
公开(公告)号：US06228626B1
公开(公告)日：2001-05-08
申请号：US09457302
申请日：1999-12-09
申请人： Toshio Ichikawa , Yasuji Koyama
发明人： Toshio Ichikawa , Yasuji Koyama
IPC分类号： C12N906
CPC分类号： C12Y105/03001 , C12N9/0034
摘要： This invention relates to sarcosine oxidase having the following physico-chemical properties: (a) action: oxidatively hydrolyzing 1 mole sarcosine to give 1 mole glycine, 1 mole formaldehyde, and 1 mole hydrogen peroxide; (b) substrate specificity: specific for sarcosine; (c) optimum pH: 7.0-8.0; (d) stable pH range: 7.0-9.5; (e) suitable temperature range for action: 50° C.; (f) thermostability: 55° C. or less; and (g) molecular weight: 44,000 daltons (when estimated roughly from the amino acid sequence of the wild-type), and to a process for producing the sarcosine oxidase, comprising the steps of culturing a microorganism having an ability to produce the sarcosine oxidase and collecting the sarcosine oxidase from the culture are provided.
摘要翻译：本发明涉及具有以下物理化学性质的肌氨酸氧化酶：（a）作用：氧化水解1摩尔肌氨酸，得到1摩尔甘氨酸，1摩尔甲醛和1摩尔过氧化氢; （b）底物特异性：肌氨酸特异性; （c）最适pH：7.0-8.0; （d）稳定的pH范围：7.0-9.5; （e）合适的温度范围：50°C; （f）热稳定性：55℃以下; 和（g）分子量：44,000道尔顿（当大致估计为野生型的氨基酸序列时）和制备肌氨酸氧化酶的方法，包括以下步骤：培养具有产生肌氨酸氧化酶能力的微生物并提供从培养物中收集肌氨酸氧化酶。

5. 发明授权

US5801036A Mutant uricase, a mutant uricase gene, a novel recombinant DNA, and a process for producing mutant uricase 失效
标题翻译：突变型尿酸酶，突变型尿酸酶基因，新型重组DNA，以及生产突变型尿酸酶的方法
公开(公告)号：US5801036A
公开(公告)日：1998-09-01
申请号：US938471
申请日：1997-09-29
申请人： Yasuji Koyama , Toshio Ichikawa
发明人： Yasuji Koyama , Toshio Ichikawa
IPC分类号： C12N15/09 , C07H21/04 , C12N9/06 , C12R1/19 , C12N1/20 , C12N15/00
CPC分类号： C12N9/0046
摘要： The present invention relates to mutant uricase containing the amino acid sequence of wild-type uricase shown in SEQ ID NO:1 wherein the 165-170th amino acids contain a mutated amino acid sequence, a mutant uricase gene coding for said uricase, a recombinant DNA having said mutant uricase gene integrated into a vector DNA, and a process for producing mutant uricase by culturing a microorganism carrying said recombinant DNA and having the ability to produce mutant uricase in a medium, and then recovering mutant uricase from the culture. The present invention provides stable mutant uricase and the gene coding for said mutant uricase, and further the process of the present invention enables efficient production of stable uricase.
摘要翻译：本发明涉及包含SEQ ID NO：1所示的野生型尿酸酶的氨基酸序列的突变型尿酸酶，其中第165-170位氨基酸含有突变型氨基酸序列，编码所述尿酸酶的突变型尿酸酶基因，重组DNA 将所述突变型尿酸酶基因整合到载体DNA中，以及通过培养携带所述重组DNA并具有在培养基中产生突变性尿酸酶的能力的微生物，然后从培养物中回收突变性尿酸酶的方法来产生突变型尿酸酶。本发明提供稳定的突变型尿酸酶和编码所述突变型尿酸酶的基因，进一步本发明的方法能够有效地产生稳定的尿酸酶。

6. 发明授权

US07070948B1 Method for assaying glycated protein 有权
标题翻译：糖化蛋白测定方法
公开(公告)号：US07070948B1
公开(公告)日：2006-07-04
申请号：US10088524
申请日：2000-09-29
申请人： Ryoichi Sakaue , Ayumi Arai , Naoki Kajiyama , Yasuji Koyama
发明人： Ryoichi Sakaue , Ayumi Arai , Naoki Kajiyama , Yasuji Koyama
IPC分类号： C12Q1/37 , C01N33/53
CPC分类号： C12Q1/26 , C12Q1/37 , G01N30/02 , Y10S435/975
摘要： Based on a principle that is different to that of a conventional enzymatic method, the present invention provides a novel method for assaying a glycated protein by a simple procedure, within a short period of time, and with high accuracy, and a reagent kit for assaying used in the method. The method for assaying a glycated protein in a sample is realized by treating a glycated protein-containing sample with protease to liberate a glycated peptide, preferably an α-glycated peptide, particularly preferably an α-glycated dipeptide, from a glycated protein, allowing an oxidase to react with the liberated glycated peptide, and determining the produced hydrogen peroxide.
摘要翻译：基于与常规酶法不同的原理，本发明提供了一种通过简单的方法在短时间内和高精度测定糖基化蛋白质的新方法和用于测定的试剂盒用于该方法。用于测定样品中糖化蛋白质的方法是通过用蛋白酶处理含糖基化蛋白的样品来实现的，以从糖基化蛋白质释放糖基化肽，优选α-糖化肽，特别优选α-糖化二肽，从而允许氧化酶与释放的糖化肽反应，并测定所产生的过氧化氢。

7. 发明授权

US5932466A Creatine amidinohydrolase gene, a novel recombinant DNA, and a process for producing creatine amidinohydrolase 失效
标题翻译：肌酸脒基水解酶基因，新型重组DNA，以及生产肌酸脒基水解酶的方法
公开(公告)号：US5932466A
公开(公告)日：1999-08-03
申请号：US947726
申请日：1997-10-09
申请人： Keisuke Furukawa , Toshio Ichikawa , Masaru Suzuki , Yasuji Koyama
发明人： Keisuke Furukawa , Toshio Ichikawa , Masaru Suzuki , Yasuji Koyama
IPC分类号： C12N15/09 , C12N1/21 , C12N9/78 , C12N9/80 , C12N15/55 , C12R1/05 , C12R1/19 , C07H21/04 , C12N15/63
CPC分类号： C12N9/80
摘要： The present invention relates to a creatine amidinohydrolase gene coding for the amino acid sequence of SEQ ID NO. 2, a recombinant DNA comprising the creatine amidinohydrolase gene inserted into a vector DNA, and a process for producing creatine amidinohydrolase by culturing a microorganism belonging to the genus Escherichia carrying said recombinant DNA and having the ability to produce creatine amidinohydrolase in medium and then recovering creatine amidinohydrolase from the culture.According to the present invention, creatine amidinohydrolase can be efficiently produced without adding creatine to medium.
摘要翻译：本发明涉及编码SEQ ID NO：1的氨基酸序列的肌酸脒基水解酶基因。 2，包含插入到载体DNA中的肌酸脒基水解酶基因的重组DNA和通过培养携带所述重组DNA并具有在培养基中产生肌酸脒基水解酶的能力然后回收肌酸的属于携带所述重组DNA的埃希氏菌属的微生物来生产肌酸脒基水解酶的方法来自培养基的脒基水解酶。根据本发明，可以在不向培养基中添加肌酸的情况下有效地制备肌酸脒基水解酶。

8. 发明授权

US5712139A Pyranose oxidase, pyranose oxidase gene, novel recombinant DNA and process for producing pyranose oxidase 失效
标题翻译：吡喃糖氧化酶，吡喃糖氧化酶基因，新型重组DNA和生产吡喃糖氧化酶的方法
公开(公告)号：US5712139A
公开(公告)日：1998-01-27
申请号：US734925
申请日：1996-10-22
申请人： Ikuko Nishimura , Kimiharu Okada , Tomoyuki Minamihara , Genshiro Kawai , Yasuji Koyama , Masaru Suzuki
发明人： Ikuko Nishimura , Kimiharu Okada , Tomoyuki Minamihara , Genshiro Kawai , Yasuji Koyama , Masaru Suzuki
IPC分类号： C12N15/09 , C12N1/21 , C12N9/04 , C12N15/36 , C12N15/53 , C12R1/01 , C12R1/19
CPC分类号： C12N9/0006 , C12Y101/0301
摘要： The invention relates to a novel pyranose oxidase, a pyranose oxidase gene, and a recombinant DNA, as well as a process for producing the pyranose oxidase by use of a microorganism carrying said recombinant DNA. The process of the invention enables efficient production of the novel pyranose oxidase.
摘要翻译：本发明涉及新型吡喃糖氧化酶，吡喃糖氧化酶基因和重组DNA，以及通过使用携带所述重组DNA的微生物来生产吡喃糖氧化酶的方法。本发明的方法能够有效地生产新型吡喃糖氧化酶。

9. 发明授权

US5700674A Mutant uricase, a mutant uricase gene, a novel recombinant DNA, and a process for producing mutant uricase 失效
标题翻译：突变型尿酸酶，突变型尿酸酶基因，新型重组DNA，以及生产突变型尿酸酶的方法
公开(公告)号：US5700674A
公开(公告)日：1997-12-23
申请号：US701952
申请日：1996-08-23
申请人： Yasuji Koyama , Toshio Ichikawa
发明人： Yasuji Koyama , Toshio Ichikawa
IPC分类号： C12N15/09 , C07H21/04 , C12N9/06 , C12R1/19 , C12N1/20 , C12P21/06
CPC分类号： C12N9/0046
摘要： The present invention relates to mutant uricase containing the amino acid sequence of wild-type uricase shown in SEQ ID NO: 1 wherein the 165-170th amino acids contain a mutated amino acid sequence, a mutant uricase gene coding for said uricase, a recombinant DNA having said mutant uricase gene integrated into a vector DNA, and a process for producing mutant uricase by culturing a microorganism carrying said recombinant DNA and having the ability to produce mutant uricase in a medium, and then recovering mutant uricase from the culture. The present invention provides stable mutant uricase and the gene coding for said mutant uricase, and further the process of the present invention enables efficient production of stable uricase.
摘要翻译：本发明涉及包含SEQ ID NO：1所示的野生型尿酸酶的氨基酸序列的突变型尿酸酶，其中第165-170位氨基酸含有突变型氨基酸序列，编码所述尿酸酶的突变型尿酸酶基因，重组DNA 将所述突变型尿酸酶基因整合到载体DNA中，以及通过培养携带所述重组DNA并具有在培养基中产生突变性尿酸酶的能力的微生物，然后从培养物中回收突变性尿酸酶的方法来产生突变型尿酸酶。本发明提供稳定的突变型尿酸酶和编码所述突变型尿酸酶的基因，进一步本发明的方法能够有效地产生稳定的尿酸酶。

10. 发明授权

US08877915B2 Transcription regulatory factors for mannanases or cellulases, and genes for the transcription regulatory factor 有权
标题翻译：甘露聚糖酶或纤维素酶的转录调节因子，以及转录调节因子的基因
公开(公告)号：US08877915B2
公开(公告)日：2014-11-04
申请号：US13202279
申请日：2010-03-02
申请人： Masahiro Ogawa , Yasuji Koyama , Masayuki Machida
发明人： Masahiro Ogawa , Yasuji Koyama , Masayuki Machida
IPC分类号： C07K14/38 , C12N9/44 , C07H21/04 , A23J1/18 , C12N9/42
CPC分类号： C12N9/2434 , C07K14/38 , C12N9/2451 , C12N9/2488
摘要： Disclosed are: transcription regulatory factors capable of regulating the transcription or expression of genes for mannanases or cellulases, as mentioned below; and others. Specifically disclosed is a protein selected from the following proteins (a), (b) and (c): (a) a protein comprising the amino acid sequence depicted in SEQ ID NO:2; (b) a protein which comprises an amino acid sequence produced by deleting, substituting or adding one or several amino acid residues (e.g., 1 to 5 amino acid residues) in the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases; and (c) a protein which comprises an amino acid sequence having a 70% or higher sequence identity to the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases, or a partial fragment of the protein. Also specifically disclosed are a gene encoding the protein, and others.
摘要翻译：公开的是：能够调节甘露聚糖酶或纤维素酶基因的转录或表达的转录调节因子，如下所述; 和别的。具体公开的是选自以下蛋白质（a），（b）和（c）的蛋白质：（a）包含SEQ ID NO：2所示氨基酸序列的蛋白质; （b）包含在SEQ ID NO：2所示的氨基酸序列中缺失，取代或添加一个或几个氨基酸残基（例如1至5个氨基酸残基）而产生的氨基酸序列的蛋白质，其能够调节甘露聚糖酶或纤维素酶基因的转录; 和（c）蛋白质，其包含与SEQ ID NO：2所示的氨基酸序列具有70％或更高序列同一性的氨基酸序列，并且能够调节甘露聚糖酶或纤维素酶的基因的转录，或部分蛋白质的片段。还具体公开了编码蛋白质的基因等。

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