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1. 发明授权

US07091017B2 Fructosyl amino acid oxidase 有权
标题翻译：果糖基氨基酸氧化酶
公开(公告)号：US07091017B2
公开(公告)日：2006-08-15
申请号：US10416537
申请日：2001-11-21
申请人： Ryoichi Sakaue , Naoki Kajiyama
发明人： Ryoichi Sakaue , Naoki Kajiyama
IPC分类号： C12N9/04 , C12N9/06 , C07H21/04
CPC分类号： C12N9/0022
摘要： The present invention provides a novel fructosyl amino acid oxidase that is excellent in thermal stability. Moreover, the present invention provides a fructosyl amino acid oxidase gene encoding the novel fructosyl amino acid oxidase, a recombinant DNA in which the gene is inserted into a vector DNA, a transformant or transductant containing this gene, and a process for producing the novel fructosyl amino acid oxidase, which comprises culturing the transformant or transductant in a medium and collecting the novel fructosyl amino acid oxidase from the culture product.
摘要翻译：本发明提供热稳定性优异的新型果糖基氨基酸氧化酶。此外，本发明提供了编码新型果糖基氨基酸氧化酶的果糖基氨基酸氧化酶基因，其中将基因插入载体DNA的重组DNA，含有该基因的转化体或转导体，以及生产新型果糖基氨基酸氧化酶，其包括在培养基中培养转化体或转导体，并从培养产物中收集新的果糖基氨基酸氧化酶。

2. 发明申请

US20070010000A1 Cholesterol oxidase stable in the presence of surfactant 有权
标题翻译：胆固醇氧化酶在表面活性剂存在下稳定
公开(公告)号：US20070010000A1
公开(公告)日：2007-01-11
申请号：US11448048
申请日：2006-06-07
申请人： Ryoichi Sakaue , Naoki Kajiyama
发明人： Ryoichi Sakaue , Naoki Kajiyama
IPC分类号： C12N9/02 , C07H21/04 , C12P21/06
CPC分类号： C12N9/0006
摘要： A novel cholesterol oxidase having stability in the presence of surfactant and a gene encoding the novel cholesterol oxidase.
摘要翻译：在表面活性剂存在下具有稳定性的新型胆固醇氧化酶和编码新型胆固醇氧化酶的基因。

3. 发明授权

US07371550B2 Cholesterol oxidase stable in the presence of surfactant 有权
标题翻译：胆固醇氧化酶在表面活性剂存在下稳定
公开(公告)号：US07371550B2
公开(公告)日：2008-05-13
申请号：US11448048
申请日：2006-06-07
申请人： Ryoichi Sakaue , Naoki Kajiyama
发明人： Ryoichi Sakaue , Naoki Kajiyama
IPC分类号： C12N9/02 , C07H21/04 , C12P21/06
CPC分类号： C12N9/0006
摘要： A novel cholesterol oxidase having stability in the presence of surfactant and a gene encoding the novel cholesterol oxidase.
摘要翻译：在表面活性剂存在下具有稳定性的新型胆固醇氧化酶和编码新型胆固醇氧化酶的基因。

4. 发明授权

US07070948B1 Method for assaying glycated protein 有权
标题翻译：糖化蛋白测定方法
公开(公告)号：US07070948B1
公开(公告)日：2006-07-04
申请号：US10088524
申请日：2000-09-29
申请人： Ryoichi Sakaue , Ayumi Arai , Naoki Kajiyama , Yasuji Koyama
发明人： Ryoichi Sakaue , Ayumi Arai , Naoki Kajiyama , Yasuji Koyama
IPC分类号： C12Q1/37 , C01N33/53
CPC分类号： C12Q1/26 , C12Q1/37 , G01N30/02 , Y10S435/975
摘要： Based on a principle that is different to that of a conventional enzymatic method, the present invention provides a novel method for assaying a glycated protein by a simple procedure, within a short period of time, and with high accuracy, and a reagent kit for assaying used in the method. The method for assaying a glycated protein in a sample is realized by treating a glycated protein-containing sample with protease to liberate a glycated peptide, preferably an α-glycated peptide, particularly preferably an α-glycated dipeptide, from a glycated protein, allowing an oxidase to react with the liberated glycated peptide, and determining the produced hydrogen peroxide.
摘要翻译：基于与常规酶法不同的原理，本发明提供了一种通过简单的方法在短时间内和高精度测定糖基化蛋白质的新方法和用于测定的试剂盒用于该方法。用于测定样品中糖化蛋白质的方法是通过用蛋白酶处理含糖基化蛋白的样品来实现的，以从糖基化蛋白质释放糖基化肽，优选α-糖化肽，特别优选α-糖化二肽，从而允许氧化酶与释放的糖化肽反应，并测定所产生的过氧化氢。

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