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1. 发明授权

US5827720A .alpha.-glucosidase, and a process for producing the same 失效
标题翻译： α-葡糖苷酶及其制备方法
公开(公告)号：US5827720A
公开(公告)日：1998-10-27
申请号：US775459
申请日：1996-12-30
申请人： Tomoyuki Minamihara , Kimiharu Okada , Masaru Suzuki
发明人： Tomoyuki Minamihara , Kimiharu Okada , Masaru Suzuki
IPC分类号： C12N9/26 , C12P19/20 , C12N1/20
CPC分类号： C12N9/2414 , C12N9/2408 , C12P19/20
摘要： The present invention relates to novel .alpha.-glucosidase being excellent in stability to heat and pH, and hydrolyzing .alpha.-glucoside linkages to form .alpha.-D-glucose, acting on maltose, maltotriose, maltotetraose, PNPG, PNPG2, isomaltose and sucrose, but hardly acting on maltooligosaccharides with a degree of polymerization of maltopentaoside or more, and completely not acting on soluble starch, as well as to a process for producing the same by use of a strain belonging to the genus Bacillus capable of producing .alpha.-glucosidase. .alpha.-Glucosidase which is useful as a coupling enzyme for quantification such as measurement of .alpha.-amylase activity, or in formation of monosaccharides such as glucose and fructose etc., from oligosaccharides, and enzymatic synthesis of heterooligosaccharides, can be produced easily and inexpensively.
摘要翻译：本发明涉及对热和pH稳定性优异的α-葡萄糖苷酶和水解α-葡萄糖苷键以形成α-D-葡萄糖，作用于麦芽糖，麦芽三糖，麦芽四糖，PNPG，PNPG2，异麦芽糖和蔗糖，但几乎不作用于具有一定程度的麦芽糖苷或更多的聚合的麦芽低聚糖，并且完全不作用于可溶性淀粉，以及通过使用属于能够产生α-葡糖苷酶的芽孢杆菌属的菌株生产该麦芽低聚糖。作为用于定量的偶联酶，例如测定α-淀粉酶活性或从低聚糖形成单糖如葡萄糖和果糖等，以及低寡糖的酶促合成可以容易且廉价地生产的α-葡糖苷酶。

2. 发明授权

US5712139A Pyranose oxidase, pyranose oxidase gene, novel recombinant DNA and process for producing pyranose oxidase 失效
标题翻译：吡喃糖氧化酶，吡喃糖氧化酶基因，新型重组DNA和生产吡喃糖氧化酶的方法
公开(公告)号：US5712139A
公开(公告)日：1998-01-27
申请号：US734925
申请日：1996-10-22
申请人： Ikuko Nishimura , Kimiharu Okada , Tomoyuki Minamihara , Genshiro Kawai , Yasuji Koyama , Masaru Suzuki
发明人： Ikuko Nishimura , Kimiharu Okada , Tomoyuki Minamihara , Genshiro Kawai , Yasuji Koyama , Masaru Suzuki
IPC分类号： C12N15/09 , C12N1/21 , C12N9/04 , C12N15/36 , C12N15/53 , C12R1/01 , C12R1/19
CPC分类号： C12N9/0006 , C12Y101/0301
摘要： The invention relates to a novel pyranose oxidase, a pyranose oxidase gene, and a recombinant DNA, as well as a process for producing the pyranose oxidase by use of a microorganism carrying said recombinant DNA. The process of the invention enables efficient production of the novel pyranose oxidase.
摘要翻译：本发明涉及新型吡喃糖氧化酶，吡喃糖氧化酶基因和重组DNA，以及通过使用携带所述重组DNA的微生物来生产吡喃糖氧化酶的方法。本发明的方法能够有效地生产新型吡喃糖氧化酶。

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