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1. 发明授权

US08980615B2 Recombinant microorganism and method for producing aliphatic polyester using the same 有权
标题翻译：重组微生物及其制备方法
公开(公告)号：US08980615B2
公开(公告)日：2015-03-17
申请号：US13636830
申请日：2011-03-24
申请人： Masayoshi Muramatsu , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda
发明人： Masayoshi Muramatsu , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda
IPC分类号： C12N1/20 , C12P7/62 , C12N9/10
CPC分类号： C12P7/625 , C12N9/1029 , C12N9/13 , C12Y203/01 , C12Y208/03001
摘要： Aliphatic polyester productivity is improved for production of aliphatic polyester using a recombinant microorganism. A recombinant microorganism prepared by introducing a gene encoding a protein having activity of converting lactic acid to lactic-acid CoA and a gene encoding a protein having activity of synthesizing polyhydroxyalkanoate using hydroxyacyl CoA as a substrate into a host microorganism is cultured and then aliphatic polyester is recovered from the medium.
摘要翻译：使用重组微生物生产脂肪族聚酯的脂肪族聚酯生产率得到改善。培养通过将编码具有将乳酸转化为乳酸CoA的蛋白质的蛋白质的基因和使用羟基酰基CoA作为底物合成聚羟基链烷酸酯的蛋白质的基因的基因导入宿主微生物而制备的重组微生物，然后将脂肪族聚酯从培养基中回收。

2. 发明授权

US08748137B2 Method for production of polylactate using recombinant microorganism 有权
标题翻译：使用重组微生物生产聚乳酸的方法
公开(公告)号：US08748137B2
公开(公告)日：2014-06-10
申请号：US13126157
申请日：2009-10-27
申请人： Shusei Obata , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda , Seiichi Taguchi
发明人： Shusei Obata , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda , Seiichi Taguchi
IPC分类号： C12P7/62 , C12N9/00 , C12N9/10 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12P7/62 , C12N9/1029
摘要： It is an object of the present invention to provide a method for efficiently producing a polylactate via microbial fermentation with the use of a sugar as a starting material.The method is a method for producing a polylactate, which comprises the steps of: (1) culturing a recombinant microorganism having a protein capable of catalyzing a reaction of transferring CoA to propionic acid and/or lactate and a protein capable of catalyzing a reaction of polyhydroxyalkanoate synthesis consisting of the following amino acid sequence (a) or (b) in a medium containing a carbon source: (a) an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of at least one of the amino acids at positions 130, 325, 477, and 481 with different amino acid(s); or (b) an amino acid sequence derived from the protein specified in (a) by additional deletion or substitution of one or several amino acid(s) other than the amino acids at positions 130, 325, 477, and 481 or by insertion of one or several amino acid residue(s); and (2) collecting the polylactate from the culture product obtained in step (1).
摘要翻译：本发明的目的是提供一种利用糖作为起始原料通过微生物发酵有效生产聚乳酸的方法。该方法是制备聚乳酸的方法，其包括以下步骤：（1）培养具有能够催化将CoA转移到丙酸和/或乳酸的反应的蛋白质的蛋白质和能够催化反应的蛋白质的重组微生物在含有碳源的培养基中由以下氨基酸序列（a）或（b）组成的聚羟基链烷酸酯合成物：（a）通过至少一个取代至少一个氨基酸序列的氨基酸序列，所述氨基酸序列来自SEQ ID NO：2所示的氨基酸序列的具有不同氨基酸的位置130,325,477和481处的氨基酸; 或（b）衍生自（a）中规定的蛋白质的氨基酸序列，通过除了位置130,325,477和481之外的氨基酸以外的一个或几个氨基酸的另外缺失或取代，或通过插入一个或几个氨基酸残基; 和（2）从步骤（1）获得的培养产物中收集聚乳酸。

3. 发明申请

US20130175351A1 EXPANSION VALVE 审中-公开
标题翻译：膨胀阀
公开(公告)号：US20130175351A1
公开(公告)日：2013-07-11
申请号：US13822184
申请日：2011-09-22
申请人： Yoshimasa Kikuchi , Shigetaka Wakisaka , Takashi Shimamura , Keiji Tomioka , Motohiko Fukuoka
发明人： Yoshimasa Kikuchi , Shigetaka Wakisaka , Takashi Shimamura , Keiji Tomioka , Motohiko Fukuoka
IPC分类号： F25B41/06
CPC分类号： F25B41/062 , F25B2500/01
摘要： An expansion valve for a refrigerant circuit of a refrigeration apparatus is configured to depressurize a liquid refrigerant. The expansion valve includes a valve main body with a valve seat opening into a valve chest formed in the valve main body and a valve body movable relative to the valve chest in a reciprocating manner. An orifice is formed in the valve seat. The orifice has an orifice entrance with a smallest diameter, an orifice middle section widening in diameter along a direction of refrigerant outflow from the orifice entrance so as to form a first taper angle, and an orifice exit either widening in diameter or not in diameter along the direction of refrigerant outflow from the orifice middle section so as to form a second taper angle smaller than the first taper angle.
摘要翻译：用于制冷装置的制冷剂回路的膨胀阀被构造成使液体制冷剂减压。膨胀阀包括阀主体，阀主体开口形成在阀主体中的阀室，阀体以往复方式相对于阀箱运动。在阀座中形成孔口。孔口具有最小直径的孔入口，沿着从孔入口流出的制冷剂方向直径变宽的孔中部，以形成第一锥角，以及直径扩大或不直径扩大的孔口制冷剂从孔中间部分流出的方向形成小于第一锥角的第二锥角。

4. 发明申请

US20130045516A1 RECOMBINANT MICROORGANISM AND METHOD FOR PRODUCING ALIPHATIC POLYESTER USING THE SAME 有权
标题翻译：重组微生物及使用其生产聚氨酯的方法
公开(公告)号：US20130045516A1
公开(公告)日：2013-02-21
申请号：US13636830
申请日：2011-03-24
申请人： Masayoshi Muramatsu , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda
发明人： Masayoshi Muramatsu , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda
IPC分类号： C12P7/62 , C12N1/21
CPC分类号： C12P7/625 , C12N9/1029 , C12N9/13 , C12Y203/01 , C12Y208/03001
摘要： Aliphatic polyester productivity is improved for production of aliphatic polyester using a recombinant microorganism. A recombinant microorganism prepared by introducing a gene encoding a protein having activity of converting lactic acid to lactic-acid CoA and a gene encoding a protein having activity of synthesizing polyhydroxyalkanoate using hydroxyacyl CoA as a substrate into a host microorganism is cultured and then aliphatic polyester is recovered from the medium.
摘要翻译：使用重组微生物生产脂肪族聚酯的脂肪族聚酯生产率得到改善。培养通过将编码具有将乳酸转化为乳酸CoA的蛋白质的蛋白质的基因和使用羟基酰基CoA作为底物合成聚羟基链烷酸酯的蛋白质的基因的基因导入宿主微生物而制备的重组微生物，然后将脂肪族聚酯从培养基中回收。

5. 发明申请

US20120122165A1 RECOMBINANT MICROORGANISM AND METHOD FOR PRODUCING ALIPHATIC POLYESTER WITH THE USE OF THE SAME 审中-公开
标题翻译：重组微生物及其使用生产聚氨酯的方法
公开(公告)号：US20120122165A1
公开(公告)日：2012-05-17
申请号：US13387271
申请日：2010-07-27
申请人： Shusei Obata , Masayoshi Muramatsu , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda
发明人： Shusei Obata , Masayoshi Muramatsu , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda
IPC分类号： C12P7/62 , C12N1/21
CPC分类号： C12N9/13 , C12N9/1029 , C12P7/62
摘要： This invention provides a recombinant microorganism exhibiting excellent aliphatic polyester productivity and a method for producing an aliphatic polyester with the use of the recombinant microorganism. In this invention, the Megasphaera elsdenii-derived pct gene (and/or the Staphylococcus aureus-derived pct gene) and the Pseudomonas sp. 61-3 strain-derived PHA synthase gene (phaC2 gene) (and/or the Alcanivorax borkumensis SK2 strain-derived PHA synthase gene) are introduced into a host microorganism.
摘要翻译：本发明提供使用重组微生物显示优异脂肪族聚酯生产率的重组微生物和生产脂肪族聚酯的方法。在本发明中，来自大疱疹病毒的巨噬细胞源基因（和/或金黄色葡萄球菌来源的pct基因）和假单胞菌属（Pseudomonas sp。 61-3菌株衍生的PHA合酶基因（phaC2基因）（和/或阿尔卡氏菌属Bclumum SK2株衍生的PHA合酶基因）引入宿主微生物中。

6. 发明申请

US20110214450A1 BITE-IN TYPE PIPE CONNECTION STRUCTURE, VALVE, BITE-IN TYPE PIPE JOINT, AND REFRIGERATION DEVICE 有权
标题翻译： BITE-IN型管道连接结构，阀门，BITE-IN型管接头和制冷装置
公开(公告)号：US20110214450A1
公开(公告)日：2011-09-08
申请号：US13128648
申请日：2010-01-15
申请人： Haruo Nakata , Takashi Shimamura
发明人： Haruo Nakata , Takashi Shimamura
IPC分类号： F25B23/00 , F16K27/00 , F16L19/08
CPC分类号： F16L19/10 , F16L19/14 , F16L2201/10
摘要： A bite-in type pipe connection structure is provided, the pipe connection structure being capable of, even if piping connection work is performed with a length of piping inserted insufficiently, minimizing deterioration of air-tightness at the joint portion, at which the piping is connected, due to an offset load acting on the piping. Also provided are a valve, a bite-in type pipe joint, and a refrigeration device using the bite-in type pipe connection structure. A ferrule 30 is provided with: a low-strength portion 34a which, when a coupling member becomes fastened to a joint body by manually rotating the coupling member, allows the distal end portion 36 of the ferrule 30 to deform so that the distal end portion 36 restrains a length of piping which is to be connected; a level changing portion 32b which connects a front tapered surface 32a, which corresponds to the low-strength portion 34a, and a rear tapered surface 32c; and a rearward portion 30A which is adjacent to the low-strength section 34a. The level changing portion 32b is formed so that the wall thickness of the rearward portion 30A has a value which can airtightly retain the piping to be connected.
摘要翻译：提供了一种咬入式管连接结构，即使管道连接作业在一段管道插入不足的情况下也能够使管道连接结构最小化，从而使配管的接头部分的气密性降低最小化由于作用在管道上的偏移负载而连接。还提供了阀，咬入式管接头和使用咬入式管连接结构的制冷装置。套管30设有：低强度部34a，当联接构件通过手动旋转联接构件而紧固到接头体时，允许套圈30的前端部36变形，使得前端部 36限制要连接的管道长度; 连接与低强度部34a对应的前锥面32a和后锥面32c的水平切换部32b，以及与低强度部34a相邻的后方部30A。水平切换部分32b形成为使得后部30A的壁厚具有可以气密地保持要连接的管道的值。

7. 发明授权

US07954856B2 Flareless pipe coupling structure, flareless pipe coupling method, and on-site pipe coupling method 有权
标题翻译：无焰管联结结构，无爆管耦合法及现场管耦合法
公开(公告)号：US07954856B2
公开(公告)日：2011-06-07
申请号：US12225113
申请日：2007-03-30
申请人： Haruo Nakata , Takashi Shimamura
发明人： Haruo Nakata , Takashi Shimamura
IPC分类号： F16L37/00
CPC分类号： F16L19/14 , F16L19/10 , F16L19/103
摘要： A guide tapered surface 17 of a pipe coupling port 16 has a small diameter portion 17a guiding to a distal end portion 3a of a bite type sleeve 3 to bite into a coupling pipe 5 and a large diameter portion 17b sealing a tapered outer circumferential surface 33 of the bite type sleeve 3 by making close contact with the tapered outer circumferential surface 33 of the bite type sleeve 3. A tilt angle α1 of the small diameter portion 17a relative to a central axis of the guide tapered surface 17 is greater than a tilt angle α2 of the large diameter portion 17b relative to the central axis. Therefore, even when a distal end portion of the coupling pipe 5 is not inserted up to an innermost portion of the pipe coupling port 16, excellent sealing performance is obtained in a portion coupled to the coupling pipe.
摘要翻译：管接头16的引导锥形表面17具有引导到咬合套筒3的远端部分3a的小直径部分17a，以咬入联接管5和密封锥形外圆周表面33的大直径部分17b 通过与咬合型套筒3的锥形外圆周表面33紧密接触，咬合型套筒3的小直径部分17a相对于导向锥形表面17的中心轴线的倾斜角α1大于倾斜大直径部分17b相对于中心轴线的角度α2。因此，即使当连接管5的前端部未插入管接头16的最内部时，也能够在与联接管连接的部分中获得良好的密封性能。

8. 发明申请

US20110104768A1 METHOD FOR PRODUCTION OF POLYESTER COPOLYMER USING RECOMBINANT MICROORGANISM 有权
标题翻译：使用重组微生物生产聚酯共聚物的方法
公开(公告)号：US20110104768A1
公开(公告)日：2011-05-05
申请号：US12989333
申请日：2009-04-23
申请人： Seiichi Taguchi , Kenji Tajima , Yasuharu Satoh , Ken'ichiro Matsumoto , Miwa Yamada , Shusei Obata , Hiromi Kambe , Katsuhiro Kohda , Katsuhiro Ohno , Takashi Shimamura
发明人： Seiichi Taguchi , Kenji Tajima , Yasuharu Satoh , Ken'ichiro Matsumoto , Miwa Yamada , Shusei Obata , Hiromi Kambe , Katsuhiro Kohda , Katsuhiro Ohno , Takashi Shimamura
IPC分类号： C12P7/62 , C12N1/00 , C12N1/21
CPC分类号： C12P7/62
摘要： The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3HB and LA via microbial fermentation with the use of a sugar as a starting material.The following is provided: a method for producing a polyester copolymer consisting of 3-hydroxybutyrate and lactate, which comprises the steps of: (1) culturing a recombinant microorganism having a protein capable of catalyzing a reaction of transferring CoA to propionic acid and/or lactate, a protein capable of catalyzing a reaction of forming acetoacetyl-CoA from two acetyl-CoA molecules, a protein capable of catalyzing a reaction of acetoacetyl-CoA reduction, and a protein capable of catalyzing a reaction of polyhydroxyalkanoate synthesis consisting of the following amino acid sequence (a) or (b) in a medium containing a carbon source: (a) an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of at least one of the amino acids at positions 130, 325, 477, and 481 with different amino acid(s); or (b) an amino acid sequence derived from the protein specified in (a) by additional deletion or substitution of at least one amino acid other than the amino acids at positions 130, 325, 477, and 481 or by insertion of at least one amino acid residue; and (2) collecting the polyester copolymer from the culture product obtained in step (1). According to the production method of the present invention, a polyester copolymer consisting of 3HB and LA can be efficiently produced using an inexpensive carbon source as a starting material, and thus the production cost of a biodegradable plastic can be reduced.
摘要翻译：本发明的目的是提供一种利用糖作为原料，通过微生物发酵有效地制备由3HB和LA组成的聚酯共聚物的方法。提供以下：由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物的制造方法，其特征在于，包括以下步骤：（1）培养具有能够催化将CoA转移到丙酸和/或乳酸盐，能够催化从两个乙酰辅酶A分子形成乙酰乙酰辅酶A的反应的蛋白质，能够催化乙酰乙酰辅酶A还原反应的蛋白质，以及能够催化由下列氨基组成的聚羟基链烷酸酯合成反应的蛋白质含有碳源的培养基中的酸序列（a）或（b）：（a）从SEQ ID NO：2所示的氨基酸序列衍生的氨基酸序列，通过将位置130的至少一个氨基酸置换，325,477和481具有不同的氨基酸; 或（b）衍生自（a）中规定的蛋白质的氨基酸序列，通过除了位置130,325,477和481之外的氨基酸以外的至少一个氨基酸的另外缺失或取代或通过插入至少一个氨基酸残基; 和（2）从步骤（1）获得的培养产物中收集聚酯共聚物。根据本发明的制造方法，可以使用廉价的碳源作为原料，有效地制造由3HB和LA组成的聚酯共聚物，从而可以降低生物降解性塑料的制造成本。

9. 发明申请

US20090309357A1 BITE-IN TYPE PIPE JOINT, REFRIGERATING APPARATUS, AND WATER HEATING DEVICE 审中-公开
标题翻译： BITE-IN型管接头，制冷装置和水加热装置
公开(公告)号：US20090309357A1
公开(公告)日：2009-12-17
申请号：US12374617
申请日：2007-08-28
申请人： Haruo Nakata , Takashi Shimamura , Jun Koizumi
发明人： Haruo Nakata , Takashi Shimamura , Jun Koizumi
IPC分类号： F16L19/06 , F16L21/06
CPC分类号： F16L19/14
摘要： A bite-in type pipe joint includes a first joint member having an internal thread and a second joint member having an external thread. An annular ferrule is formed integrally with the second joint member. By the time the first and second joint members are fastened to each other, the ferrule separates from the second joint member and bites into a pipe. The second joint member has a cylindrical cover that covers the ferrule. This structure prevents a distal portion and a tapered surface of the ferrule from being damaged when the joint member is handled. A refrigerating apparatus and/or a water heating device can include one or more of the pipe joints.
摘要翻译：咬入式管接头包括具有内螺纹的第一接头构件和具有外螺纹的第二接头构件。环形套圈与第二接头构件一体地形成。当第一和第二接头构件彼此紧固时，套圈与第二接头构件分离并咬入管中。第二接头构件具有覆盖套圈的圆柱形盖。这种结构防止了当接合构件被处理时套管的远端部分和锥形表面被损坏。制冷装置和/或水加热装置可以包括一个或多个管接头。

10. 发明申请

US20090267343A1 PIPE JOINT, REFRIGERATION DEVICE, AND HEAT PUMP-TYPE HOT WATER DEVICE 审中-公开
标题翻译：管接头，制冷装置和热泵型热水装置
公开(公告)号：US20090267343A1
公开(公告)日：2009-10-29
申请号：US12441360
申请日：2007-09-28
申请人： Haruo Nakata , Takashi Shimamura , Jun Koizumi
发明人： Haruo Nakata , Takashi Shimamura , Jun Koizumi
IPC分类号： F16L49/00
CPC分类号： F16L19/04 , F16L19/00 , F16L19/005 , F16L19/10 , F16L19/14
摘要： A pipe joint includes a joint body 1 into which a pipe 4 is to be inserted and a coupling member 2 which is arranged around the pipe 4 to be inserted into the joint body 1. The joint body 1 and the coupling member 2 include screw portions 14a, 21a, respectively. The coupling member 2 is fastened to the joint body 1 by mutually screwing the screw portions 14a, 21a. The coupling member 2 includes a holding portion 22. A pair of or a plurality of pairs of holding surfaces which are opposite to each other are formed on the outer circumferential surface of the holding portion 22 so that the holding portion 22 can be held by a fastening tool. A part of the coupling member 2 including at least the holding surfaces is disconnectable from the remaining part of the coupling member 2.
摘要翻译：管接头包括要插入管4的接头体1和布置在管4周围以插入接头本体1中的接合构件2.接头体1和接头构件2包括螺纹部分 14a，21a。联接构件2通过相互拧紧螺纹部14a，21a而紧固到接头本体1。联接构件2包括保持部22.在保持部22的外周面上形成有彼此相对的一对或多对保持面，使得保持部22能够被保持紧固工具。包括至少保持表面的联接构件2的一部分可与联接构件2的其余部分分离。

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