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1. 发明申请

US20110104768A1 METHOD FOR PRODUCTION OF POLYESTER COPOLYMER USING RECOMBINANT MICROORGANISM 有权
标题翻译：使用重组微生物生产聚酯共聚物的方法
公开(公告)号：US20110104768A1
公开(公告)日：2011-05-05
申请号：US12989333
申请日：2009-04-23
申请人： Seiichi Taguchi , Kenji Tajima , Yasuharu Satoh , Ken'ichiro Matsumoto , Miwa Yamada , Shusei Obata , Hiromi Kambe , Katsuhiro Kohda , Katsuhiro Ohno , Takashi Shimamura
发明人： Seiichi Taguchi , Kenji Tajima , Yasuharu Satoh , Ken'ichiro Matsumoto , Miwa Yamada , Shusei Obata , Hiromi Kambe , Katsuhiro Kohda , Katsuhiro Ohno , Takashi Shimamura
IPC分类号： C12P7/62 , C12N1/00 , C12N1/21
CPC分类号： C12P7/62
摘要： The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3HB and LA via microbial fermentation with the use of a sugar as a starting material.The following is provided: a method for producing a polyester copolymer consisting of 3-hydroxybutyrate and lactate, which comprises the steps of: (1) culturing a recombinant microorganism having a protein capable of catalyzing a reaction of transferring CoA to propionic acid and/or lactate, a protein capable of catalyzing a reaction of forming acetoacetyl-CoA from two acetyl-CoA molecules, a protein capable of catalyzing a reaction of acetoacetyl-CoA reduction, and a protein capable of catalyzing a reaction of polyhydroxyalkanoate synthesis consisting of the following amino acid sequence (a) or (b) in a medium containing a carbon source: (a) an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of at least one of the amino acids at positions 130, 325, 477, and 481 with different amino acid(s); or (b) an amino acid sequence derived from the protein specified in (a) by additional deletion or substitution of at least one amino acid other than the amino acids at positions 130, 325, 477, and 481 or by insertion of at least one amino acid residue; and (2) collecting the polyester copolymer from the culture product obtained in step (1). According to the production method of the present invention, a polyester copolymer consisting of 3HB and LA can be efficiently produced using an inexpensive carbon source as a starting material, and thus the production cost of a biodegradable plastic can be reduced.
摘要翻译：本发明的目的是提供一种利用糖作为原料，通过微生物发酵有效地制备由3HB和LA组成的聚酯共聚物的方法。提供以下：由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物的制造方法，其特征在于，包括以下步骤：（1）培养具有能够催化将CoA转移到丙酸和/或乳酸盐，能够催化从两个乙酰辅酶A分子形成乙酰乙酰辅酶A的反应的蛋白质，能够催化乙酰乙酰辅酶A还原反应的蛋白质，以及能够催化由下列氨基组成的聚羟基链烷酸酯合成反应的蛋白质含有碳源的培养基中的酸序列（a）或（b）：（a）从SEQ ID NO：2所示的氨基酸序列衍生的氨基酸序列，通过将位置130的至少一个氨基酸置换，325,477和481具有不同的氨基酸; 或（b）衍生自（a）中规定的蛋白质的氨基酸序列，通过除了位置130,325,477和481之外的氨基酸以外的至少一个氨基酸的另外缺失或取代或通过插入至少一个氨基酸残基; 和（2）从步骤（1）获得的培养产物中收集聚酯共聚物。根据本发明的制造方法，可以使用廉价的碳源作为原料，有效地制造由3HB和LA组成的聚酯共聚物，从而可以降低生物降解性塑料的制造成本。

2. 发明授权

US5966472A Method for automatic recognition of a concavity or convexity 失效
标题翻译：自动识别凹凸的方法
公开(公告)号：US5966472A
公开(公告)日：1999-10-12
申请号：US906172
申请日：1997-08-05
申请人： Takayuki Kataoka , Ken'ichiro Matsumoto
发明人： Takayuki Kataoka , Ken'ichiro Matsumoto
IPC分类号： G01B11/00 , G06K9/46 , G06T7/60 , G06K9/36 , G06K9/00 , G06K9/56
CPC分类号： G06K9/4604
摘要： An automatic recognition method capable of accurately determining edges of a concavity or convexity in order to achieve an improved measuring accuracy in recognition of a concavity or convexity. This method comprises the steps of: converting a photographic image of an object having a concavity or convexity defined therein into data in a specified feature space; obtaining a plurality of points that exist outside a virtual circle determined by a virtual center of the concavity or convexity, the virtual center being established for each elementary area of the image converted into the feature space data; obtaining the equation of a plane that passes through the plurality of points; detecting symmetric pairs of pixels in each elementary area of the feature space data with respect to the corresponding virtual concavity or convexity center; and recognizing the position of the concavity or convexity of the object when the symmetric pixels satisfy the equation of the plane.
摘要翻译：一种能够精确地确定凹凸的边缘的自动识别方法，以便在识别凹凸时获得提高的测量精度。该方法包括以下步骤：将其中定义的具有凹凸的物体的摄影图像转换成指定特征空间中的数据; 获得存在于由所述凹凸的虚拟中心确定的虚拟圆之外的多个点，为被转换为所述特征空间数据的图像的每个基本区域建立所述虚拟中心; 获得通过所述多个点的平面的方程; 相对于相应的虚拟凹凸中心检测特征空间数据的每个基本区域中的对称对像对; 并且当对称像素满足平面的方程时，识别对象的凹凸的位置。

3. 发明授权

US08563281B2 Method for production of polyester copolymer using recombinant microorganism 有权
标题翻译：使用重组微生物生产聚酯共聚物的方法
公开(公告)号：US08563281B2
公开(公告)日：2013-10-22
申请号：US12989333
申请日：2009-04-23
申请人： Seiichi Taguchi , Kenji Tajima , Yasuharu Satoh , Ken'ichiro Matsumoto , Miwa Yamada , Shusei Obata , Hiromi Kambe , Katsunori Kohda , Katsuhiro Ohno , Takashi Shimamura
发明人： Seiichi Taguchi , Kenji Tajima , Yasuharu Satoh , Ken'ichiro Matsumoto , Miwa Yamada , Shusei Obata , Hiromi Kambe , Katsunori Kohda , Katsuhiro Ohno , Takashi Shimamura
IPC分类号： C12P7/62 , C12N1/20 , C12N1/00
CPC分类号： C12P7/62
摘要： The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3-hydroxybutyrate and lactate via microbial fermentation with the use of a sugar as a starting material. The method may comprise culturing a recombinant microorganism expressing: a protein capable of catalyzing the transfer of CoA to propionic acid and/or lactate; a protein capable of catalyzing the formation of acetoacetyl-CoA from two acetyl-CoA molecules; a protein capable of catalyzing acetoacetyl-CoA reduction; and a protein capable of catalyzing polyhydroxyalkanoate synthesis. According to the production method, a polyester copolymer consisting of 3-hydroxybutyrate and lactate can be efficiently produced using an inexpensive carbon source as a starting material, and thus the production cost of a biodegradable plastic can be reduced.
摘要翻译：本发明的目的是提供一种通过使用糖作为原料通过微生物发酵有效地制备由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物的方法。该方法可以包括培养表达：能够催化CoA转移到丙酸和/或乳酸盐的蛋白质的重组微生物; 能够催化从两个乙酰辅酶A分子形成乙酰乙酰辅酶A的蛋白质; 能够催化乙酰乙酰辅酶A还原的蛋白质; 和能够催化聚羟基链烷酸酯合成的蛋白质。根据该制造方法，可以使用廉价的碳源作为起始材料有效地制造由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物，从而可以降低生物降解性塑料的制造成本。

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