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1. 发明申请

WO2006133182A2 INSECT-SPECIFIC PROTEASE RECOGNITION SEQUENCES 审中-公开
标题翻译： INSECT-SPECIFIC PROTEASE识别序列
公开(公告)号：WO2006133182A2
公开(公告)日：2006-12-14
申请号：PCT/US2006/021902
申请日：2006-06-06
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , ABAD, Andre R. , CHUN, Gary C. , FLANNAGAN, Ronald D. , HERRMANN, Rafael , LU, Albert L. , MCCUTCHEN, Billy F. , RICE, Janet A. , SCHEPERS, Eric J.
发明人： ABAD, Andre R. , CHUN, Gary C. , FLANNAGAN, Ronald D. , HERRMANN, Rafael , LU, Albert L. , MCCUTCHEN, Billy F. , RICE, Janet A. , SCHEPERS, Eric J.
IPC分类号： C07K14/325 , C12N9/64 , A01N63/02 , C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , C12N9/6472 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.
摘要翻译：提供了保护植物免受害虫侵害的组合物和方法。提供编码昆虫原毒素或昆虫毒素的核酸分子，其被修饰为包含至少一个对昆虫肠道蛋白酶敏感的蛋白水解活化位点。通过昆虫肠蛋白酶在蛋白水解活化位点切割修饰的昆虫原毒素在害虫的肠道中产生活性昆虫毒素。在蛋白水解活化位点切割本发明的修饰的昆虫毒素导致在昆虫肠中产生活性昆虫毒素，其相对于缺乏蛋白水解活化位点的昆虫毒素显示出改善的杀虫活性。提供了使用修饰的昆虫原毒素和修饰的昆虫毒素核酸序列及其编码的多肽以保护植物免受害虫的方法。本发明的具体实施方案还提供了改良的昆虫原毒素和改良的昆虫毒素组合物和制剂，表达盒和转化的植物，植物细胞和种子。昆虫肠道蛋白酶和编码它们的核酸分子也在本文中公开。

2. 发明申请

WO2005066349A3 GENES ENCODING PROTEINS WITH PESTICIDAL ACTIVITY 审中-公开
标题翻译：用杀虫活性编码蛋白质的基因
公开(公告)号：WO2005066349A3
公开(公告)日：2005-10-27
申请号：PCT/US2004041530
申请日：2004-12-09
申请人： PIONEER HI BRED INT , DU PONT , ABAD ANDRE , DONG HUA , HERRMANN RAFAEL , LU ALBERT , MCCUTCHEN BILLY F , RICE JANET A , SCHEPERS ERIC J , WONG JAMES F
发明人： ABAD ANDRE , DONG HUA , HERRMANN RAFAEL , LU ALBERT , MCCUTCHEN BILLY F , RICE JANET A , SCHEPERS ERIC J , WONG JAMES F
IPC分类号： C07K14/325 , C12N15/82
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. The invention provides mutagenized nucleic acids that have been engineered to encode pesticidal polypeptides having increased resistance to proteolytic degradation by a plant protease. In particular, nucleic acid sequences encoding pesticidal polypeptides modified to comprise a proteolytic protection site that confers resistance to degradation or proteolytic inactivation by a plant protease are provided. Particular embodiments of the invention provide pesticidal polypeptide compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. These compositions find use in methods for controlling pests, especially plant pests. Novel plant proteases, sequences encoding these proteases, and methods for their use are also provided.
摘要翻译：提供了保护植物免受害虫害虫的组合物和方法。本发明提供已被工程化以编码具有增加的抗植物蛋白酶对蛋白水解降解的杀虫多肽的诱变的核酸。特别地，提供了编码杀虫多肽的核酸序列，其被修饰为包含赋予植物蛋白酶降解或蛋白水解灭活抗性的蛋白水解保护位点。本发明的具体实施方案提供杀虫多肽组合物和制剂，表达盒和转化植物，植物细胞和种子。这些组合物可用于防治害虫，特别是植物害虫的方法。还提供了新型植物蛋白酶，编码这些蛋白酶的序列及其使用方法。

3. 发明申请

WO2005066349A2 GENES ENCODING PROTEINS WITH PESTICIDAL ACTIVITY 审中-公开
标题翻译：编码具有杀虫活性的蛋白质的基因
公开(公告)号：WO2005066349A2
公开(公告)日：2005-07-21
申请号：PCT/US2004/041530
申请日：2004-12-09
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY , ABAD, André , DONG, Hua , HERRMANN, Rafael , LU, Albert , McCUTCHEN, Billy, F. , RICE, Janet, A. , SCHEPERS, Eric, J. , WONG, James, F.
发明人： ABAD, André , DONG, Hua , HERRMANN, Rafael , LU, Albert , McCUTCHEN, Billy, F. , RICE, Janet, A. , SCHEPERS, Eric, J. , WONG, James, F.
IPC分类号： C12N15/82
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. The invention provides mutagenized nucleic acids that have been engineered to encode pesticidal polypeptides having increased resistance to proteolytic degradation by a plant protease. In particular, nucleic acid sequences encoding pesticidal polypeptides modified to comprise a proteolytic protection site that confers resistance to degradation or proteolytic inactivation by a plant protease are provided. Particular embodiments of the invention provide pesticidal polypeptide compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. These compositions find use in methods for controlling pests, especially plant pests. Novel plant proteases, sequences encoding these proteases, and methods for their use are also provided.
摘要翻译：提供了用于保护植物免受害虫侵害的组合物和方法。本发明提供了经过工程改造以编码对植物蛋白酶的蛋白水解降解具有增强的抗性的杀虫多肽的诱变核酸。具体而言，提供了编码杀有害生物多肽的核酸序列，其修饰成包含赋予对植物蛋白酶的降解或蛋白水解失活的抗性的蛋白水解保护位点。本发明的具体实施方案提供了杀有害生物多肽组合物和制剂，表达盒和转化植物，植物细胞和种子。这些组合物可用于防治害虫特别是植物害虫的方法中。还提供了新的植物蛋白酶，编码这些蛋白酶的序列及其使用方法。

4. 发明申请

WO2009091862A3 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LEPIDOPTERA 审中-公开
公开(公告)号：WO2009091862A3
公开(公告)日：2009-07-23
申请号：PCT/US2009/031055
申请日：2009-01-15
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DUPONT DE NEMOURS & COMPANY , HERRMANN, Rafael , LASSNER, Michael , LU, Albert L. , NELSON, Mark , PRESNAIL, James K. , RICE, Janet A.
发明人： HERRMANN, Rafael , LASSNER, Michael , LU, Albert L. , NELSON, Mark , PRESNAIL, James K. , RICE, Janet A.
IPC分类号： C12N15/82
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lepidoptera order, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides set forth in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Spodoptera frugiperda. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

5. 发明申请

WO2006133182A3 INSECT-SPECIFIC PROTEASE RECOGNITION SEQUENCES 审中-公开
标题翻译： INSECT-SPECIFIC PROTEASE识别序列
公开(公告)号：WO2006133182A3
公开(公告)日：2007-02-08
申请号：PCT/US2006021902
申请日：2006-06-06
申请人： PIONEER HI BRED INT , DU PONT , ABAD ANDRE R , CHUN GARY C , FLANNAGAN RONALD D , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , RICE JANET A , SCHEPERS ERIC J
发明人： ABAD ANDRE R , CHUN GARY C , FLANNAGAN RONALD D , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , RICE JANET A , SCHEPERS ERIC J
IPC分类号： C07K14/325 , A01N63/02 , C12N9/64 , C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , C12N9/6472 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.
摘要翻译：提供了保护植物免受害虫害虫的组合物和方法。提供编码昆虫原毒素或昆虫毒素的核酸分子，其被修饰为包含至少一个对昆虫肠道蛋白酶敏感的蛋白水解活化位点。通过昆虫肠蛋白酶在蛋白水解活化位点切割修饰的昆虫原毒素在害虫的肠道中产生活性昆虫毒素。在蛋白水解活化位点切割本发明的修饰的昆虫毒素导致在昆虫肠中产生活性昆虫毒素，其相对于缺乏蛋白水解活化位点的昆虫毒素显示出改善的杀虫活性。提供了使用修饰的昆虫原毒素和修饰的昆虫毒素核酸序列及其编码的多肽以保护植物免受害虫的方法。本发明的具体实施方案还提供改性的昆虫原毒素和修饰的昆虫毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。昆虫肠道蛋白酶和编码它们的核酸分子也在本文中公开。

6. 发明申请

WO2006014316A1 ANTIFUNGAL POLYPEPTIDES 审中-公开
标题翻译：抗菌多糖
公开(公告)号：WO2006014316A1
公开(公告)日：2006-02-09
申请号：PCT/US2005/023339
申请日：2005-07-01
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMUORS AND COMPANY , THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , ALTIER, Daniel, J., Altier , DAHLBACKA, Glen , HERRMANN, Rafael , HUNTER-CEVERA, Jennie , McCUTCHEN, Billy, Fred , PRESNAIL, James, K. , SCHEPERS, Eric, J. , RICE, Janet, A. , SIMMONS, Carl, R. , TOROK, Tamas , YALPANI, Nasser
发明人： ALTIER, Daniel, J., Altier , DAHLBACKA, Glen , HERRMANN, Rafael , HUNTER-CEVERA, Jennie , McCUTCHEN, Billy, Fred , PRESNAIL, James, K. , SCHEPERS, Eric, J. , RICE, Janet, A. , SIMMONS, Carl, R. , TOROK, Tamas , YALPANI, Nasser
IPC分类号： C07K14/385 , C07K14/37
CPC分类号： C07K14/37 , A01N63/02 , A01N63/04 , A61K38/00 , C07K14/385 , C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.
摘要翻译：提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

7. 发明申请

WO2005063996A2 PLANT ACTIVATION OF INSECT TOXIN 审中-公开
标题翻译：昆虫毒素的植物激活
公开(公告)号：WO2005063996A2
公开(公告)日：2005-07-14
申请号：PCT/US2004/042736
申请日：2004-12-20
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY , ABAD, André , FLANNAGAN, Ronald, D. , HERRMANN, Rafael , LU, Albert , MCCUTCHEN, Billy, F. , PRESNAIL, James, K. , RICE, Janet, A. , WONG, James, F. , YU, Cao-Guo
发明人： ABAD, André , FLANNAGAN, Ronald, D. , HERRMANN, Rafael , LU, Albert , MCCUTCHEN, Billy, F. , PRESNAIL, James, K. , RICE, Janet, A. , WONG, James, F. , YU, Cao-Guo
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
摘要翻译：提供了用于保护植物免受害虫侵害的组合物和方法。具体而言，提供了编码经修饰以包含至少一个对植物蛋白酶或昆虫肠蛋白酶敏感的蛋白水解活化位点的昆虫原毒素的核酸序列。在蛋白水解活化位点被蛋白酶切割修饰的原毒素产生活性昆虫毒素。提供了使用修饰的昆虫原毒素核酸序列和它们编码的多肽来保护植物免受害虫的方法。本发明的特定实施方案进一步提供了修饰的昆虫原毒素组合物和制剂，表达盒和转化的植物，植物细胞和种子。

8. 发明申请

WO2009091864A3 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LYGUS 审中-公开
标题翻译：抑制淋巴瘤靶向多核苷酸的组合物和方法
公开(公告)号：WO2009091864A3
公开(公告)日：2009-12-10
申请号：PCT/US2009031057
申请日：2009-01-15
申请人： PIONEER HI BRED INT , DU PONT , HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
发明人： HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N57/16 , C12N15/113 , C12N15/8218 , C12N2310/141 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lygus genus, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides from specific polypeptide families as disclosed herein, and further provides target polynucleotides set forth in SEQ ID NOS:1-21 or active variants thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Lygus Hesperus. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉默元件的方法和组合物，所述沉默元件当被有害生物如来自草盲蝽属的害虫摄入时，能够降低害虫中靶序列的表达。在具体的实施方案中，靶序列表达的降低控制害虫，并且由此该方法和组合物能够限制对植物的损害。本发明提供了来自本文公开的特定多肽家族的各种靶多核苷酸，并且还提供了SEQ ID NO：1-21中列出的靶多核苷酸或其活性变体，其中靶害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性）。进一步提供了沉默元件，其在被害虫摄入时降低靶多肽的水平并由此控制害虫。在具体的实施方案中，害虫是草盲蝽（Lygus Hesperus）。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其他宿主细胞。

9. 发明申请

WO2009091860A3 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM THE FAMILY APHIDIDAE 审中-公开
标题翻译：用于抑制来自家庭蚜虫目标多核苷酸的组合物和方法
公开(公告)号：WO2009091860A3
公开(公告)日：2009-11-05
申请号：PCT/US2009031053
申请日：2009-01-15
申请人： PIONEER HI BRED INT , DU PONT , HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
发明人： HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , C07K14/43563 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Aphididae family, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides for specific protein classes and also target polynucleotides as set forth in SEQ ID NOS:1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or active variants or fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Aphis glycines. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉默元件的方法和组合物，所述沉默元件当被有害生物例如来自蚜科的害虫摄取时，能够降低害虫中靶序列的表达。在具体的实施方案中，靶序列表达的降低控制害虫，并且由此该方法和组合物能够限制对植物的损害。本发明提供了特定蛋白质类别的靶多核苷酸以及如SEQ ID NO：1,2,3,4,5,6,7,8,9,10或其活性变体或片段所示的靶多核苷酸，其中a 目标害虫中一个或多个序列表达的减少控制了害虫（即，具有杀虫活性）。进一步提供了沉默元件，其在被害虫摄入时降低靶多肽的水平并由此控制害虫。在具体的实施方案中，害虫是蚜虫（Aphis）甘氨酸。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其他宿主细胞。

10. 发明申请

WO2009091864A2 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LYGUS 审中-公开
标题翻译：用于抑制来自LYGUS的目标多核苷酸的组合物和方法
公开(公告)号：WO2009091864A2
公开(公告)日：2009-07-23
申请号：PCT/US2009/031057
申请日：2009-01-15
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , HERRMANN, Rafael , LASSNER, Michael , LU, Albert, L. , NELSON, Mark , PRESNAIL, James, K. , RICE, Janet, A.
发明人： HERRMANN, Rafael , LASSNER, Michael , LU, Albert, L. , NELSON, Mark , PRESNAIL, James, K. , RICE, Janet, A.
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N57/16 , C12N15/113 , C12N15/8218 , C12N2310/141 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lygus genus, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides from specific polypeptide families as disclosed herein, and further provides target polynucleotides set forth in SEQ ID NOS:1-21 or active variants thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Lygus Hesperus. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当沉积元件被害虫摄取时，例如来自莱古氏属属的害虫，它们能够降低害虫中靶序列的表达。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供了本文公开的来自特定多肽家族的多种靶多核苷酸，并进一步提供了SEQ ID NO：1-21所示的靶多核苷酸或其活性变体，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性）。进一步提供的是沉积元件，当被害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，有害生物是黄瓜（Lygus Hesperus）。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

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