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1. 发明申请

WO2013019411A1 METHODS AND COMPOSITIONS FOR TARGETED INTEGRATION IN A PLANT 审中-公开
标题翻译：方法和组合在工厂中进行集中整合
公开(公告)号：WO2013019411A1
公开(公告)日：2013-02-07
申请号：PCT/US2012/047202
申请日：2012-07-18
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL , CIGAN, Andrew Mark , FALCO, Saverio Carl , GAO, Huirong , LASSNER, Michael W. , LI, Zhongsen , LIU, Zhan-Bin , LYZNIK, Leszek A. , PETERSON, David J. , SCELONGE, Christopher Jay
发明人： CIGAN, Andrew Mark , FALCO, Saverio Carl , GAO, Huirong , LASSNER, Michael W. , LI, Zhongsen , LIU, Zhan-Bin , LYZNIK, Leszek A. , PETERSON, David J. , SCELONGE, Christopher Jay
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8213
摘要： Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.
摘要翻译：提供组合物和方法用于将目的多核苷酸序列靶向整合到植物或植物细胞的基因组中。方法和组合物使用核酸内切酶和内切核酸酶的识别位点与位点特异性重组位点/重组酶组合，以提供用于在植物，植物细胞或种子的基因组内建立靶位点的有效系统。一旦建立了这样的靶位点，就可以采用多种方法来进一步修饰靶位点，使得它们含有多种感兴趣的多核苷酸。

2. 发明申请

WO2012129373A2 METHODS FOR PRODUCING A COMPLEX TRANSGENIC TRAIT LOCUS 审中-公开
标题翻译：用于生产复合转基因位置的方法
公开(公告)号：WO2012129373A2
公开(公告)日：2012-09-27
申请号：PCT/US2012/030061
申请日：2012-03-22
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY , LASSNER, Michael , JANTZ, Derek , SMITH, James, Jefferson , CIGAN, Mark , FALCO, Carl , GAO, Huirong , LI, Zhongsen , LIU, Zhan-bin , SVITASHEV, Sergei
发明人： LASSNER, Michael , JANTZ, Derek , SMITH, James, Jefferson , CIGAN, Mark , FALCO, Carl , GAO, Huirong , LI, Zhongsen , LIU, Zhan-bin , SVITASHEV, Sergei
IPC分类号： C12N15/82 , A01H1/06 , A01H5/10 , C12N15/55
CPC分类号： C12N15/8202 , A01H1/06 , C12N9/22 , C12N15/8213
摘要： Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break- inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
摘要翻译：公开了在植物中生产复合转基因性状基因座的方法，所述基因座包含在目的基因组区域中的至少两个改变的靶序列。所述方法涉及使用两种或更多种双链断裂诱导剂，其中每一种可导致目的基因组区域中靶序列中的双链断裂，导致目标序列的改变。还公开了植物中的复杂的转基因性状基因座。复杂的转基因性状基因座包含与感兴趣的多核苷酸遗传连锁的至少两个改变的靶序列。还公开了植物，植物细胞，植物部分和包含一个或多个复合转基因性状基因座的种子。

3. 发明申请

WO2005123929A2 PLASTID TRANSIT PEPTIDES 审中-公开
标题翻译：塑料过滤胶
公开(公告)号：WO2005123929A2
公开(公告)日：2005-12-29
申请号：PCT/US2005/020455
申请日：2005-06-09
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , LASSNER, Michael , WILKINSON, Jack, Q.
发明人： LASSNER, Michael , WILKINSON, Jack, Q.
IPC分类号： C12N15/82
CPC分类号： C07K14/415 , C07K14/00 , C12N15/8221
摘要： The present invention provides novel plastid transit peptides that direct lacalization of attached moieties (e.g., polypeptides) into plant plastids. The present invension also relates to methods and compositions for lacalizing polypeptides to plant plastids including, but not limited to, transgenic plant production.
摘要翻译：本发明提供了将附着部分（例如，多肽）引导到植物质体中的新型质体转运肽。本发明还涉及用于将多肽拉染植物质体的方法和组合物，包括但不限于转基因植物生产。

4. 发明申请

WO9533055A3 WAX ESTERS IN TRANSFORMED PLANTS 审中-公开
标题翻译：变形植物中的花生
公开(公告)号：WO9533055A3
公开(公告)日：1996-01-04
申请号：PCT/US9506671
申请日：1995-06-01
申请人： CALGENE INC , LASSNER MICHAEL , METZ JAMES GEORGE
发明人： LASSNER MICHAEL , METZ JAMES GEORGE
IPC分类号： A01H1/00 , C11B1/00 , C11B11/00 , C11C1/00 , C12N5/10 , C12N9/02 , C12N9/04 , C12N15/09 , C12N15/53 , C12N15/82 , C12P7/64
CPC分类号： C12N9/0008 , C11B11/00 , C12N15/8247 , C12Y102/01042
摘要： The invention provides a method of producing a wax ester in a plant cell whereby a plant cell having a fatty acyl reductase expressed from a sequence heterologous to said plant is grown in the absence of a wax synthase expressed from a sequence which is heterologous to the plant. The invention also provides plant cells containing wax ester.
摘要翻译：本发明提供了一种在植物细胞中生产蜡酯的方法，其中具有由与所述植物异源的序列表达的脂肪酰基还原酶的植物细胞在不存在由与植物异源的序列表达的蜡合酶时生长。本发明还提供含有蜡酯的植物细胞。

5. 发明申请

WO2009091862A2 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LEPIDOPTERA 审中-公开
标题翻译：用于抑制来自LEPIDOPTERA的目标多核苷酸的组合物和方法
公开(公告)号：WO2009091862A2
公开(公告)日：2009-07-23
申请号：PCT/US2009031055
申请日：2009-01-15
申请人： PIONEER HI BRED INT , DU PONT , HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
发明人： HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N65/20 , A01N65/44 , C12N15/113 , C12N2310/11 , C12N2310/14 , C12N2310/531 , C12N2320/32 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lepidoptera order, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides set forth in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Spodoptera frugiperda. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当沉积元件在被害虫如来自鳞翅目序列的害虫中摄取时，它们能够降低害虫中靶序列的表达。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供编码来自特定蛋白质家族的多肽的多核苷酸和SEQ ID NO：1,2,3,4,5,6,7,8,9,10,11,12,13,14中所示的各种靶多核苷酸的靶多核苷酸，15，16，17，18，19，20，21，22，23，24，25，26，27，28，29，30，31，32，33，34，35，36，37，38，39 ，40,41,42,43,44,45,46,47,48,49或50个或其活性变体和片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即，具有杀虫活性）。进一步提供的是沉积元件，当被害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，害虫是草地夜蛾（Spodoptera frugiperda）。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

6. 发明申请

WO2009091860A2 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM THE FAMILY APHIDIDAE 审中-公开
标题翻译：从家禽APHIDIDA中抑制目标多核苷酸的组合物和方法
公开(公告)号：WO2009091860A2
公开(公告)日：2009-07-23
申请号：PCT/US2009/031053
申请日：2009-01-15
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , HERRMANN, Rafael , LASSNER, Michael , LU, Albert, L. , NELSON, Mark , PRESNAIL, James, K. , RICE, Janet, A.
发明人： HERRMANN, Rafael , LASSNER, Michael , LU, Albert, L. , NELSON, Mark , PRESNAIL, James, K. , RICE, Janet, A.
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , C07K14/43563 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Aphididae family, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides for specific protein classes and also target polynucleotides as set forth in SEQ ID NOS:1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or active variants or fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Aphis glycines. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当沉积元件在被害虫摄入时，例如来自蚜科的害虫中，它们能够降低害虫中靶序列的表达。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供了用于特异性蛋白质类别的靶多核苷酸以及如SEQ ID NO：1,2,3,4,5,6,7,8,9,10或其活性变体或片段所示的靶多核苷酸，其中a 目标害虫中一个或多个序列的表达减少对害虫的控制（即具有杀虫活性）。进一步提供的是沉积元件，当被害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，害虫是蚜虫甘氨酸。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

7. 发明申请

WO2006085966A3 METHODS OF PROTECTING PLANTS FROM PATHOGENIC FUNGI AND NEMATODES 审中-公开
标题翻译：从病原真菌和NEMATODES保护植物的方法
公开(公告)号：WO2006085966A3
公开(公告)日：2007-06-07
申请号：PCT/US2005023325
申请日：2005-06-30
申请人： PIONEER HI BRED INT , LASSNER MICHAEL , WU GUSUI , MULLER MATHIAS L , KEENAN ROBERT J , ALI HANA , SHAH GOWRI , WEI JUN-ZHI
发明人： LASSNER MICHAEL , WU GUSUI , MULLER MATHIAS L , KEENAN ROBERT J , ALI HANA , SHAH GOWRI , WEI JUN-ZHI
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8285 , C07K14/415 , C12N15/8282 , Y02A40/164
摘要： Methods for protecting a plant from a pathogen, particularly a pathogenic fungus or nematode, are provided. A method for enhancing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the embodiments, or variant or fragment thereof, are also disclosed.
摘要翻译：提供了保护植物免受病原体，特别是病原真菌或线虫的方法。还提供了使用本文公开的核苷酸序列增强植物中病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。还公开了包含编码实施方案的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

8. 发明申请

WO0185909A3 DEVELOPMENT OF PLANT BASED BIOLOGICAL SENSORS AND NOVEL DISEASE RESISTANCE STRATEGIES 审中-公开
标题翻译：基于植物生物传感器和新型抗病抗性策略的开发
公开(公告)号：WO0185909A3
公开(公告)日：2002-03-21
申请号：PCT/US0114419
申请日：2001-05-04
申请人： MAXYGEN INC , LASSNER MICHAEL , ENGLISH JAMES , WU GUSUI
发明人： LASSNER MICHAEL , ENGLISH JAMES , WU GUSUI
IPC分类号： C12N15/10 , C12N15/82 , C12Q1/68 , A01H5/00 , C07H21/04 , C12N15/63
CPC分类号： C12N15/1027 , C12N15/8203 , C12N15/8205 , C12N15/8283 , Y02A90/24
摘要： Methods for producing and identifying plant disease resistance (R) genes and elicitors with novel and desirable characteristics are provided. Methods for producing such R genes and elicitors using RNA recombination procedures are provided. Bio-detectors that are reporter genes responsive to induction by plant R genes, and the plant R genes that induce them are provided.
摘要翻译：提供了具有新颖和理想特性的用于产生和鉴定植物病害抗性（R）基因和诱导子的方法。提供了使用RNA重组方法产生此类R基因和诱导子的方法。提供了响应于植物R基因诱导的报告基因的生物检测器和诱导它们的植物R基因。

9. 发明申请

WO0138504A2 HOMOLOGOUS RECOMBINATION IN PLANTS 审中-公开
标题翻译：植物中的同种异体重组
公开(公告)号：WO0138504A2
公开(公告)日：2001-05-31
申请号：PCT/US0032289
申请日：2000-11-22
申请人： MAXYGEN INC , LASSNER MICHAEL , DELCARDAYRE STEVEN
发明人： LASSNER MICHAEL , DELCARDAYRE STEVEN
IPC分类号： C07K14/195 , C07K14/245 , C12N9/00 , C12N15/82 , C12N15/90
CPC分类号： C07K14/195 , C07K14/245 , C07K2319/00 , C12N9/00 , C12N15/8205 , C12N15/8213 , C12N15/902
摘要： Methods for evolving recombinase protein homologues and RecA/VirE2 fusion proteins which complement VirE2 deficient Agrobacterium are provided. The use of recombinase protein homologues and RecA/VirE2 fusion proteins in the context of Agrobacterium mediated transformation are provided. Methods for producing transgenic organisms by homologous recombination using evolved recombinase proteins and Agrobacterium strains which express recombinase protein homologues or RecA/VirE2 fusion proteins are provided. Transgenic cells and organisms which have integrated an exogenous DNA sequence into a predetermined site in their genome are provided.
摘要翻译：提供了重组酶蛋白同源物和补体VirE2缺陷型农杆菌的RecA / VirE2融合蛋白的方法。提供了在农杆菌介导转化的背景下使用重组酶蛋白同源物和RecA / VirE2融合蛋白。提供了使用进化型重组酶蛋白同源重组产生转基因生物的方法和表达重组酶蛋白同源物或RecA / VirE2融合蛋白的农杆菌菌株。提供了将外源DNA序列整合到其基因组中的预定位点的转基因细胞和生物体。

10. 发明申请

WO1995027791A1 PLANT LYSOPHOSPHATIDIC ACID ACYLTRANSFERASES 审中-公开
标题翻译：植物性脂肪酸脂肪酸酯
公开(公告)号：WO1995027791A1
公开(公告)日：1995-10-19
申请号：PCT/US1995003997
申请日：1995-03-31
申请人： CALGENE INC. , DAVIES, Huw, Maelor , HAWKINS, Deborah , NELSEN, Janet , LASSNER, Michael
发明人： CALGENE INC.
IPC分类号： C12N15/54
CPC分类号： C12N9/1029 , C12N15/8247
摘要： This invention relates to plant LPAATs, means to identify such proteins, amino acid and nucleic acid sequences associated with such protein, and methods to obtain, make and/or use such plant LPAATs. Purification, especially the removal of plant membranes and the substantial separation away from other plant proteins, and use of the plant LPAAT is provided, including the use of the protein as a tool in gene isolation for biotechnological applications. In addition, nucleic acid sequences encoding LPAAT protein regions are provided, and uses of such sequences for isolation of LPAAT genes from plants and for modification of plant triglyceride compositions are described.
摘要翻译：本发明涉及植物LPAAT，鉴定此类蛋白质的方法，与此类蛋白质相关的氨基酸和核酸序列，以及获得，制备和/或使用此类植物LPAAT的方法。净化，特别是植物膜的去除以及远离其它植物蛋白质的大量分离，并且提供了植物LPAAT的使用，包括使用蛋白质作为用于生物技术应用的基因分离的工具。另外，提供了编码LPAAT蛋白质区域的核酸序列，并描述了这些序列用于从植物中分离LPAAT基因和修饰植物甘油三酯组合物的用途。

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