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1. 发明申请

WO2012161982A1 CHLOROPLAST TRANSIT PEPTIDES AND METHODS OF THEIR USE 审中-公开
标题翻译：氯霉素转运肽及其使用方法
公开(公告)号：WO2012161982A1
公开(公告)日：2012-11-29
申请号：PCT/US2012/037360
申请日：2012-05-10
申请人： E. I. DUPONT DE NEMOURS & COMPANY , BOURETT, Timothy M. , CHUN, Gary C. , LU, Albert L.
发明人： BOURETT, Timothy M. , LU, Albert L.
IPC分类号： C07K14/415 , C12N15/82 , A01H5/00
CPC分类号： C07K14/415 , C12N15/8221
摘要： Methods and compositions are provided for targeting a polypeptide of interest to a chloroplast. Recombinant polynucleotides comprising a nucleotide sequence encoding a chimeric chloroplast transit peptide (CTP) operably linked to a heterologous polynucleotide of interest are provided. In specific embodiments, the chimeric CTP comprises an N-terminal domain operably linked to a central domain operably linked to a C-terminal domain of a CTP to form a chimeric chloroplast transit peptide having CTP activity. Recombinant polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the recombinant polynucleotides. Methods of use of the various sequences are also provided.
摘要翻译：提供了将目的多肽靶向叶绿体的方法和组合物。提供了包含编码与目的异源多核苷酸有效连接的嵌合叶绿体转运肽（CTP）的核苷酸序列的重组多核苷酸。在具体实施方案中，嵌合CTP包含可操作地连接到可操作地连接到CTP的C-末端结构域的中心结构域的N-末端结构域，以形成具有CTP活性的嵌合叶绿体转运肽。还提供了编码其的重组多肽，以及细胞，植物细胞，植物和种子，其包含重组多核苷酸。还提供了使用各种序列的方法。

2. 发明申请

WO2006133182A3 INSECT-SPECIFIC PROTEASE RECOGNITION SEQUENCES 审中-公开
标题翻译： INSECT-SPECIFIC PROTEASE识别序列
公开(公告)号：WO2006133182A3
公开(公告)日：2007-02-08
申请号：PCT/US2006021902
申请日：2006-06-06
申请人： PIONEER HI BRED INT , DU PONT , ABAD ANDRE R , CHUN GARY C , FLANNAGAN RONALD D , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , RICE JANET A , SCHEPERS ERIC J
发明人： ABAD ANDRE R , CHUN GARY C , FLANNAGAN RONALD D , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , RICE JANET A , SCHEPERS ERIC J
IPC分类号： C07K14/325 , A01N63/02 , C12N9/64 , C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , C12N9/6472 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.
摘要翻译：提供了保护植物免受害虫害虫的组合物和方法。提供编码昆虫原毒素或昆虫毒素的核酸分子，其被修饰为包含至少一个对昆虫肠道蛋白酶敏感的蛋白水解活化位点。通过昆虫肠蛋白酶在蛋白水解活化位点切割修饰的昆虫原毒素在害虫的肠道中产生活性昆虫毒素。在蛋白水解活化位点切割本发明的修饰的昆虫毒素导致在昆虫肠中产生活性昆虫毒素，其相对于缺乏蛋白水解活化位点的昆虫毒素显示出改善的杀虫活性。提供了使用修饰的昆虫原毒素和修饰的昆虫毒素核酸序列及其编码的多肽以保护植物免受害虫的方法。本发明的具体实施方案还提供改性的昆虫原毒素和修饰的昆虫毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。昆虫肠道蛋白酶和编码它们的核酸分子也在本文中公开。

3. 发明申请

WO2006133182A2 INSECT-SPECIFIC PROTEASE RECOGNITION SEQUENCES 审中-公开
标题翻译： INSECT-SPECIFIC PROTEASE识别序列
公开(公告)号：WO2006133182A2
公开(公告)日：2006-12-14
申请号：PCT/US2006/021902
申请日：2006-06-06
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , ABAD, Andre R. , CHUN, Gary C. , FLANNAGAN, Ronald D. , HERRMANN, Rafael , LU, Albert L. , MCCUTCHEN, Billy F. , RICE, Janet A. , SCHEPERS, Eric J.
发明人： ABAD, Andre R. , CHUN, Gary C. , FLANNAGAN, Ronald D. , HERRMANN, Rafael , LU, Albert L. , MCCUTCHEN, Billy F. , RICE, Janet A. , SCHEPERS, Eric J.
IPC分类号： C07K14/325 , C12N9/64 , A01N63/02 , C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , C12N9/6472 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.
摘要翻译：提供了保护植物免受害虫侵害的组合物和方法。提供编码昆虫原毒素或昆虫毒素的核酸分子，其被修饰为包含至少一个对昆虫肠道蛋白酶敏感的蛋白水解活化位点。通过昆虫肠蛋白酶在蛋白水解活化位点切割修饰的昆虫原毒素在害虫的肠道中产生活性昆虫毒素。在蛋白水解活化位点切割本发明的修饰的昆虫毒素导致在昆虫肠中产生活性昆虫毒素，其相对于缺乏蛋白水解活化位点的昆虫毒素显示出改善的杀虫活性。提供了使用修饰的昆虫原毒素和修饰的昆虫毒素核酸序列及其编码的多肽以保护植物免受害虫的方法。本发明的具体实施方案还提供了改良的昆虫原毒素和改良的昆虫毒素组合物和制剂，表达盒和转化的植物，植物细胞和种子。昆虫肠道蛋白酶和编码它们的核酸分子也在本文中公开。

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