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    • 5. 发明申请
    • Method of preparing immunoregulatory dendritic cells and the use thereof
    • 免疫调节性树突状细胞的制备方法及其用途
    • US20040235162A1
    • 2004-11-25
    • US10740834
    • 2003-12-22
    • KIRIN BEER KABUSHIKI KAISHAKatsuaki SATO
    • Katsuaki Sato
    • C12N005/08
    • C12N5/064A61K2035/122A61K2035/124C12N2501/15C12N2501/23
    • This invention provides: a therapeutic agent for graft rejection, graft-versus-host disease, autoimmune disease, allergic disease, or other diseases comprising dendritic cells (DCs) induced under culture conditions comprising both IL-10 and TGF-null or DCs prepared by adding inflammatory stimulation (e.g., TNF-null or LPS) to the aforementioned DCs and, if necessary, an antigen associated with a target disease; a method of inducing human immunoregulatory dendritic cells by culturing human dendritic cells or their precursor cells in vitro with cytokines comprising at least IL-10 and TGF-null; human immunoregulatory dendritic cells obtained by such method; and a pharmaceutical composition comprising such human immunoregulatory dendritic cells.
    • 本发明提供:用于移植排斥,移植物抗宿主病,自身免疫性疾病,过敏性疾病或包括在包含IL-10和TGF-β或DC的培养条件下诱导的树突细胞(DC)的其它疾病的治疗剂,所述培养条件由 向上述DC加入炎症刺激(如TNF-α或LPS),如有必要,加入与目标疾病相关的抗原; 通过在体外用包含至少IL-10和TGF-β的细胞因子培养人树突状细胞或其前体细胞来诱导人免疫调节性树突状细胞的方法; 通过这种方法获得的人免疫调节性树突状细胞; 和包含这种人免疫调节性树突状细胞的药物组合物。