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1. 发明授权

US5616480A Temperature sensitive plasmid 失效
标题翻译：温度敏感质粒
公开(公告)号：US5616480A
公开(公告)日：1997-04-01
申请号：US408188
申请日：1995-03-22
申请人： Masakazu Sugimoto , Hiroyuki Kojima , Akiko Tanaka , Hiroshi Matsui , Katsuaki Sato , Tsuyoshi Nakamatsu
发明人： Masakazu Sugimoto , Hiroyuki Kojima , Akiko Tanaka , Hiroshi Matsui , Katsuaki Sato , Tsuyoshi Nakamatsu
IPC分类号： C12N15/09 , C12N1/21 , C12N9/00 , C12N9/12 , C12N15/63 , C12N15/77 , C12P13/08 , C12P13/22 , C12R1/13 , C12N1/20
CPC分类号： C12N9/00 , C12N15/77 , C12N9/12 , C12P13/08 , C12P13/22
摘要： A plasmid having a temperature-sensitive replication origin which has the following properties:a) capable of autonomous replication in Corynebacterium and being retained in Corynebacterium; and,b) when a cell containing said plasmid is cultured at 31.degree. to 37.degree. C., replication of the plasmid is inhibited and at the same time, the plasmid is removed from the cell body; and a method for performing homologous recombination in Corynebacterium using the plasmid.
摘要翻译：具有温度敏感复制起点的质粒，其具有以下性质：a）能够在棒状杆菌中自主复制并保留在棒状杆菌中; 和b）当含有所述质粒的细胞在31℃至37℃下培养时，质粒的复制被抑制，同时从细胞体中除去质粒; 以及使用该质粒在棒状杆菌中进行同源重组的方法。

2. 发明授权

US5756347A Temperature-sensitive plasmid 失效
标题翻译：温度敏感质粒
公开(公告)号：US5756347A
公开(公告)日：1998-05-26
申请号：US766488
申请日：1996-12-13
申请人： Masakazu Sugimoto , Hiroyuki Kojima , Akiko Tanaka , Hiroshi Matsui , Katsuaki Sato , Tsuyoshi Nakamatsu
发明人： Masakazu Sugimoto , Hiroyuki Kojima , Akiko Tanaka , Hiroshi Matsui , Katsuaki Sato , Tsuyoshi Nakamatsu
IPC分类号： C12N15/09 , C12N1/21 , C12N9/00 , C12N9/12 , C12N15/63 , C12N15/77 , C12P13/08 , C12P13/22 , C12R1/13
CPC分类号： C12N9/00 , C12N15/77 , C12N9/12 , C12P13/08 , C12P13/22
摘要： A plasmid having a temperature-sensitive replication origin which has the following properties: a) capable of autonomous replication in Corynebacterium and being retained in Corynebacterium; and, b) when a cell containing said plasmid is cultured at 31.degree. to 37.degree. C., replication of the plasmid is inhibited and at the same time, the plasmid is removed from the cell body; and a method for performing homologous recombination in Corynebacterium using the plasmid.
摘要翻译：具有温度敏感复制起点的质粒，其具有以下性质：a）能够在棒状杆菌中自主复制并保留在棒状杆菌中; 和b）当含有所述质粒的细胞在31℃至37℃下培养时，质粒的复制被抑制，同时从细胞体中除去质粒; 以及使用质粒在棒状杆菌中进行同源重组的方法。

3. 发明授权

US5766925A Method of producing L-lysine 失效
标题翻译：生产L-赖氨酸的方法
公开(公告)号：US5766925A
公开(公告)日：1998-06-16
申请号：US700359
申请日：1996-10-08
申请人： Masakazu Sugimoto , Yoshihiro Usuda , Tomoko Suzuki , Akiko Tanaka , Hiroshi Matsui
发明人： Masakazu Sugimoto , Yoshihiro Usuda , Tomoko Suzuki , Akiko Tanaka , Hiroshi Matsui
IPC分类号： C12N9/02 , C12N9/12 , C12N15/53 , C12P13/08 , C12N1/20 , C12N9/04
CPC分类号： C12N9/0004 , C12N9/1217 , C12P13/08
摘要： A coryneform bacterium having high L-lysine productivity is provided by integrating a gene coding for aspartokinase originating from coryneform bacteria with desensitized feedback inhibition by L-lysine and L-threonine, into chromosomal DNA of a coryneform bacterium harboring leaky type homoserine dehydrogenase or a coryneform bacterium deficient in homoserine dehydrogenase gene.
摘要翻译： PCT No.PCT / JP95 / 00268 Sec。 371日期1996年10月8日第 102（e）日期1996年10月8日PCT提交1995年2月23日PCT公布。出版物WO95 / 23864 日期1995年9月8日具有高L-赖氨酸生产力的棒状细菌通过将编码来自棒状细菌的天冬氨酸激酶的基因与L-赖氨酸和L-苏氨酸的脱敏反馈抑制基因整合到含有漏型的棒状杆菌型细菌的染色体DNA中来提供高丝氨酸脱氢酶或缺失高丝氨酸脱氢酶基因的棒状细菌。

4. 发明授权

US5688671A Mutant aspartokinase gene 失效
标题翻译：突变型天冬氨酸激酶基因
公开(公告)号：US5688671A
公开(公告)日：1997-11-18
申请号：US532828
申请日：1995-10-27
申请人： Masakazu Sugimoto , Yuri Ogawa , Tomoko Suzuki , Akiko Tanaka , Hiroshi Matsui
发明人： Masakazu Sugimoto , Yuri Ogawa , Tomoko Suzuki , Akiko Tanaka , Hiroshi Matsui
IPC分类号： C12N9/12 , C12N15/09 , C12N15/54 , C12P13/08 , C12R1/13 , C12R1/15
CPC分类号： C12N9/1217 , C12N9/12 , C12P13/08
摘要： Recombinant DNA replicable in microorganisms belonging to the genus Corynebacterium, which contains a DNA fragment coding for an aspartokinase .alpha.-subunit protein originating from a bacterium belonging to the genus Corynebacterium, in which synergistic feedback inhibition by L-lysine and L-threonine is substantially desensitized, and a DNA fragment coding for an aspartokinase .beta.-subunit protein originating from a bacterium belonging to the genus Corynebacterium, in which synergistic feedback inhibition by L-lysine and L-threonine is substantially desensitized, is introduced into a microorganism belonging to the genus Corynebacterium. Thus a transformant having enhanced production and excretion speeds of L-lysine is obtained. The transformant is cultivated in an appropriate medium, and produced L-lysine is separated.
摘要翻译： PCT No.PCT / JP93 / 01809 Sec。 371 1995年10月27日第 102（e）日期1995年10月27日PCT提交1993年12月14日PCT公布。出版物WO94 / 25605 日期1994年11月10日在棒状杆菌属的微生物中可复制的重组DNA，其含有编码源自属于棒状杆菌属的细菌的天冬氨酸激酶α亚基蛋白的DNA片段，其中L-赖氨酸和L的协同反馈抑制 - 苏氨酸基本脱敏，编码源于属于棒状杆菌属的细菌的天冬氨酸激酶β亚基蛋白的DNA片段，其中L-赖氨酸和L-苏氨酸的协同反馈抑制基本上脱敏，被引入微生物属于棒杆菌属。因此获得具有增强的L-赖氨酸的产生和排泄速度的转化体。将转化体在合适的培养基中培养，分离出L-赖氨酸。

5. 发明授权

US5804414A Method of amplifying genes using artificial transposons in coryneform bacteria 失效
标题翻译：在棒状细菌中使用人工转座子扩增基因的方法
公开(公告)号：US5804414A
公开(公告)日：1998-09-08
申请号：US674168
申请日：1996-07-01
申请人： Mika Moriya , Hiroshi Matsui , Kenzo Yokozeki , Seiko Hirano , Atsushi Hayakawa , Masako Izui , Masakazu Sugimoto
发明人： Mika Moriya , Hiroshi Matsui , Kenzo Yokozeki , Seiko Hirano , Atsushi Hayakawa , Masako Izui , Masakazu Sugimoto
IPC分类号： C12N1/21 , C12N9/02 , C12N9/12 , C12N9/88 , C12N15/67 , C12N15/77 , C12N15/87 , C12N15/90 , C12P13/04 , C12P13/08 , C12P21/00 , C12P21/04
CPC分类号： C12N9/001 , C12N15/67 , C12N15/77 , C12N15/90 , C12N9/1217 , C12N9/88 , C12P13/08
摘要： A method of amplifying a desired gene in a chromosome of a coryneform bacterium, which comprises forming an artificial transposon in which a drug resistance gene and the desired gene are inserted into an insertion sequence of the coryneform bacterium, and introducing said artificial transposon into the coryneform bacterium. In accordance with the method of the present invention, a desired gene can be amplified in a chromosome in coryneform bacteria which are used in the industrial production of amino acids or nucleic acids.
摘要翻译：一种扩增棒状细菌染色体中所需基因的方法，其特征在于包括形成其中将耐药基因和所需基因插入棒状细菌的插入序列的人工转座子，并将所述人工转座子引入棒状细胞细菌。根据本发明的方法，可以在用于工业生产氨基酸或核酸的棒状细菌的染色体中扩增所需的基因。

6. 发明授权

US5876983A Mutant phosphoenolpyruvate carboxylase, its gene, and production method of amino acid 失效
标题翻译：突变型磷酸烯醇丙酮酸羧化酶，其基因和氨基酸的制备方法
公开(公告)号：US5876983A
公开(公告)日：1999-03-02
申请号：US596366
申请日：1996-04-29
申请人： Masakazu Sugimoto , Tomoko Suzuki , Hiroshi Matsui , Katsura Izui
发明人： Masakazu Sugimoto , Tomoko Suzuki , Hiroshi Matsui , Katsura Izui
IPC分类号： C12N9/88 , C12P13/04 , C12P13/06 , C12P13/08 , C12P13/14 , C12P13/20 , C12P13/12 , C12P13/24
CPC分类号： C12P13/08 , C12N9/88 , C12P13/04 , C12P13/06 , C12P13/14 , C12P13/20
摘要： A phosphoenolpyruvate carboxylase gene, which has mutation such as mutation to replace 625th glutamic acid from the N-terminus of phosphoenolpyruvate carboxylase with lysine, mutation to replace 438th arginine from the N-terminus with cysteine and the like, is introduced into Escherichia coli or coryneform bacteria, so as to produce a phosphoenolpyruvate carboxylase which is not substantially inhibited by aspartic acid, thereby amino acid is efficiently produced.
摘要翻译： PCT No.PCT / JP94 / 01365 Sec。 371日期：1996年4月29日 102（e）日期1996年4月29日PCT 1994年8月17日PCT PCT。公开号WO95 / 06114 日期：1995年3月2日引入具有从赖氨酸的磷酸烯醇丙酮酸羧化酶的N末端取代625位谷氨酸的突变等突变的磷酸烯醇式丙酮酸羧化酶基因，从N-末端用半胱氨酸等替代第438位精氨酸的突变体进入大肠杆菌或棒状细菌，以产生天冬氨酸基本上不被抑制的磷酸烯醇丙酮酸羧化酶，从而有效地产生氨基酸。

7. 发明授权

US5919694A Mutant phosphoenolpyruvate carboxylase, its gene, and production method of amino acid 失效
标题翻译：突变型磷酸烯醇丙酮酸羧化酶，其基因和氨基酸的制备方法
公开(公告)号：US5919694A
公开(公告)日：1999-07-06
申请号：US967104
申请日：1997-11-10
申请人： Masakazu Sugimoto , Tomoko Suzuki , Hiroshi Matsui , Katsura Izui
发明人： Masakazu Sugimoto , Tomoko Suzuki , Hiroshi Matsui , Katsura Izui
IPC分类号： C12N9/88 , C12P13/04 , C12P13/06 , C12P13/08 , C12P13/14 , C12P13/20 , C07H21/04 , C12N1/20
CPC分类号： C12P13/08 , C12N9/88 , C12P13/04 , C12P13/06 , C12P13/14 , C12P13/20
摘要： A phosphoenolpyruvate carboxylase gene, which has mutation such as mutation to replace 625th glutamic acid from the N-terminus of phosphoenolpyruvate carboxylase with lysine, mutation to replace 438th arginine from the N-terminus with cysteine and the like, is introduced into Escherichia coli or coryneform bacteria, so as to produce a phosphoenolpyruvate carboxylase which is not substantially inhibited by aspartic acid, thereby amino acid is efficiently produced.
摘要翻译：将具有从赖氨酸的磷酸烯醇丙酮酸羧化酶的N-末端替代第625位谷氨酸的突变等突变的磷酸烯醇丙酮酸羧化酶基因引入到大肠杆菌或棒状杆菌中，从N末端用半胱氨酸取代第438位精氨酸等细菌，以产生基本上不被天冬氨酸抑制的磷酸烯醇丙酮酸羧化酶，从而有效地产生氨基酸。

8. 发明授权

US08754326B2 Photoelectric conversion device 有权
标题翻译：光电转换装置
公开(公告)号：US08754326B2
公开(公告)日：2014-06-17
申请号：US13093374
申请日：2011-04-25
申请人： Katsuhiro Doi , Kenichi Okada , Hiroshi Matsui
发明人： Katsuhiro Doi , Kenichi Okada , Hiroshi Matsui
IPC分类号： H01G9/20 , H01M14/00
CPC分类号： H01G9/2077 , H01G9/2004 , H01G9/2022 , H01G9/2027 , H01G9/2031 , H01G9/2059 , H01M14/005 , Y02E10/542
摘要： The present invention provides a photoelectric conversion device in which changes in photoelectric conversion efficiency with time can be inhibited. The photoelectric conversion device according to the present invention includes: a pair of electrodes; an electrolytic solution disposed between the pair of electrodes; and a sealing portion that links the pair of electrodes and is provided around the electrolytic solution. At least part of the sealing portion includes at least one inorganic sealing portion constituted by an inorganic material and at least one resin sealing portion constituted by a material including a resin. The inorganic sealing portion and the resin sealing portion are disposed along a direction connecting the pair of electrodes.
摘要翻译：本发明提供可抑制光电转换效率随时间变化的光电转换装置。根据本发明的光电转换装置包括：一对电极; 设置在所述一对电极之间的电解液; 以及连接所述一对电极并设置在所述电解液周围的密封部。密封部的至少一部分包括由无机材料构成的至少一个无机密封部和由包含树脂的材料构成的至少一个树脂密封部。无机密封部和树脂密封部沿着连接该对电极的方向配置。

9. 发明授权

US08629346B2 Electrode substrate, photoelectric conversion element, conductive glass substrate and production method thereof, and pigment sensitizing solar cell 有权
标题翻译：电极基板，光电转换元件，导电玻璃基板及其制造方法以及颜料敏化太阳能电池
公开(公告)号：US08629346B2
公开(公告)日：2014-01-14
申请号：US10529818
申请日：2003-10-03
申请人： Hiroshi Matsui , Kenichi Okada , Takuya Kawashima , Nobuo Tanabe , Tetsuya Ezure
发明人： Hiroshi Matsui , Kenichi Okada , Takuya Kawashima , Nobuo Tanabe , Tetsuya Ezure
IPC分类号： H01L31/0224 , H01G9/20
CPC分类号： H01G9/2068 , H01G9/2031 , H01L51/445 , Y02E10/542 , Y02P70/521
摘要： In an electrode substrate 1, the surface of a metal circuit layer 12 is covered and insulated by an insulating layer 14. In a photoelectric conversion element that uses this electrode substrate 1, the metal circuit layer is reliably shielded from an electrolyte solution or the like so that corrosion and leak current thereof is effectively prevented, and the photoelectric conversion efficiency can be improved. The insulating layer 14 is preferably made of a material that contains a glass component, and is particularly preferably formed by printing a paste that contains glass frit. The metal circuit layer 12 is preferably formed using a printing method.
摘要翻译：在电极基板1中，金属电路层12的表面被绝缘层14覆盖并绝缘。在使用该电极基板1的光电转换元件中，金属电路层可靠地屏蔽电解液等从而有效地防止其腐蚀和漏电流，并且可以提高光电转换效率。绝缘层14优选由含有玻璃成分的材料构成，特别优选通过印刷含有玻璃料的糊料形成。金属电路层12优选使用印刷法形成。

10. 发明授权

US08524452B2 Measurement value lowering inhibitor for immunoassay method and immunoassay method using the same 有权
标题翻译：免疫测定法的测定值降低抑制剂及使用其的免疫测定法
公开(公告)号：US08524452B2
公开(公告)日：2013-09-03
申请号：US10590785
申请日：2005-02-25
申请人： Yasunori Minakawa , Michie Saito , Hiroshi Matsui
发明人： Yasunori Minakawa , Michie Saito , Hiroshi Matsui
IPC分类号： G01N33/53 , G01N33/531 , G01N33/536
CPC分类号： G01N33/52
摘要： Disclosed is an agent for inhibiting decrease in measured values in immunoassays, which may reduce the influences by interfering substances in a test sample so as to promote the accuracy of the immunoassays, as well as an immunoassay and a reagent for immunoassays using the same, with which the decrease in the measured values due to the interfering substances is reduced. The agent for inhibiting decrease in measured values in immunoassays, caused by interfering substances, is an ionic surfactant having a molecular weight of 1000 to 100,000, the agent being a polymer in which a hydrophobic cyclic monomer(s) having an ionic functional group(s) is(are) polymerized.
摘要翻译：公开了一种用于抑制免疫测定中测量值降低的试剂，其可以减少受试样品中的干扰物质的影响，从而提高免疫测定的准确性，以及免疫测定和使用其的免疫测定试剂，由于干扰物质导致的测量值的降低减少。用于抑制由干扰物质引起的免疫测定值的降低的试剂是分子量为1000〜100,000的离子性表面活性剂，该试剂是具有离子官能团的疏水性环状单体（s））被聚合。

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