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1. 发明授权

US08232055B2 Comparative genomic hybridization assays using immobilized oligonucleotide features and compositions for practicing the same 有权
标题翻译：使用固定的寡核苷酸特征和组合物进行比较的基因组杂交测定
公开(公告)号：US08232055B2
公开(公告)日：2012-07-31
申请号：US10744595
申请日：2003-12-22
申请人： Laurakay Bruhn , Alicia F. Scheffer , Michael T. Barrett , Douglas A. Amorese , Stephen S. Laderman
发明人： Laurakay Bruhn , Alicia F. Scheffer , Michael T. Barrett , Douglas A. Amorese , Stephen S. Laderman
IPC分类号： C12Q1/68 , G01N33/50
CPC分类号： C12Q1/6837 , C12Q1/6809 , C12Q2565/501
摘要： Comparative genomic hybridization assays and compositions for use in practicing the same are provided. A characteristic of the subject comparative genomic hybridization assays is that solid support immobilized oligonucleotide feature elements, e.g., in the form of an array, are employed. Specifically, at least first and second nucleic acid populations prepared from genomic templates are contacted with a plurality of distinct oligonucleotide feature elements immobilized on a solid support surface and the binding of the at least first and second populations is then evaluated. Also provided are kits for use in practicing the subject methods.
摘要翻译：提供了比较基因组杂交测定法和用于实践其的组合物。本发明比较基因组杂交测定法的特征在于使用固体支持物固定的寡核苷酸特征元件，例如阵列形式。具体地，从基因组模板制备的至少第一和第二核酸群体与固定在固体支持物表面上的多个不同的寡核苷酸特征元件接触，然后评估至少第一和第二群体的结合。还提供了用于实践主题方法的套件。

2. 发明申请

US20120231968A1 Comparative Genomic Hybridization Assays Using Immobilized Oligonucleotide Features and Compositions for Practicing the Same 审中-公开
标题翻译：使用固定的寡核苷酸特征和组合物进行比较的基因组杂交测定
公开(公告)号：US20120231968A1
公开(公告)日：2012-09-13
申请号：US13480348
申请日：2012-05-24
申请人： Laurakay Bruhn , Alicia F. Scheffer , Michael T. Barrett , Douglas A. Amorese , Stephen S. Laderman
发明人： Laurakay Bruhn , Alicia F. Scheffer , Michael T. Barrett , Douglas A. Amorese , Stephen S. Laderman
IPC分类号： C40B30/04
CPC分类号： C12Q1/6837 , C12Q1/6809 , C12Q2565/501
摘要： Comparative genomic hybridization assays and compositions for use in practicing the same are provided. A characteristic of the subject comparative genomic hybridization assays is that solid support immobilized oligonucleotide feature elements, e.g., in the form of an array, are employed. Specifically, at least first and second nucleic acid populations prepared from genomic templates are contacted with a plurality of distinct oligonucleotide feature elements immobilized on a solid support surface and the binding of the at least first and second populations is then evaluated. Also provided are kits for use in practicing the subject methods.
摘要翻译：提供了比较基因组杂交测定法和用于实践其的组合物。本发明比较基因组杂交测定法的特征在于使用固体支持物固定的寡核苷酸特征元件，例如阵列形式。具体地，从基因组模板制备的至少第一和第二核酸群体与固定在固体支持物表面上的多个不同的寡核苷酸特征元件接触，然后评估至少第一和第二群体的结合。还提供了用于实践主题方法的套件。

3. 发明授权

US07537936B2 Method of testing multiple fluid samples with multiple biopolymer arrays 失效
标题翻译：用多个生物聚合物阵列测试多个流体样品的方法
公开(公告)号：US07537936B2
公开(公告)日：2009-05-26
申请号：US11136227
申请日：2005-05-23
申请人： SueAnn C. Dahm , Arthur Schleifer , Carol T. Schembri , Douglas A. Amorese
发明人： SueAnn C. Dahm , Arthur Schleifer , Carol T. Schembri , Douglas A. Amorese
IPC分类号： G01N30/00
CPC分类号： B01L3/502 , B01L3/5025 , B01L7/00 , B01L2200/027 , B01L2200/0689 , B01L2300/0636 , B01L2300/0803 , B01L2300/0822 , B01L2300/0877 , B01L2400/0487 , Y10T436/2575
摘要： A method of testing multiple fluid samples with multiple biopolymer arrays. A cover is assembled to a contiguous substrate carrying on a first side, multiple arrays each with multiple regions of biopolymers linked to the substrate, such that the cover and the substrate together form a plurality of chambers each containing a biopolymer array and each being accessible through its own port. Multiple fluid samples are introduced into respective chambers through a port of each such that the fluid samples contact respective arrays. A binding pattern of the arrays is observed. An apparatus and kit useful in such methods, are also provided.
摘要翻译：用多个生物聚合物阵列测试多个流体样品的方法。将盖组装到承载在第一侧上的连续基底，多个阵列，每个阵列具有连接到基底的多个生物聚合区域，使得盖和基底一起形成多个腔室，每个腔室包含生物聚合物阵列，并且每个腔室可通过自己的港口多个流体样品通过各自的端口引入相应的室中，使得流体样品与相应的阵列接触。观察到阵列的绑定模式。还提供了一种在这些方法中有用的装置和试剂盒。

4. 发明授权

US07247497B2 Testing multiple fluid samples with multiple biopolymer arrays 有权
标题翻译：用多个生物聚合物阵列测试多个流体样品
公开(公告)号：US07247497B2
公开(公告)日：2007-07-24
申请号：US10160620
申请日：2002-05-31
申请人： SueAnn C. Dahm , Arthur Schleifer , Carol T. Schembri , Douglas A. Amorese
发明人： SueAnn C. Dahm , Arthur Schleifer , Carol T. Schembri , Douglas A. Amorese
IPC分类号： G01N30/00
CPC分类号： B01L3/5025 , B01L3/5027 , B01L9/52 , B01L2200/027 , B01L2200/0689 , B01L2300/044 , B01L2300/0636 , B01L2300/0803 , B01L2300/0816 , B01L2300/0877 , B01L2400/0487 , Y10T436/2575
摘要： A method of testing multiple fluid samples with multiple biopolymer arrays. A cover is assembled to a contiguous substrate carrying on a first side, multiple arrays each with multiple regions of biopolymers linked to the substrate, such that the cover and the substrate together form a plurality of chambers each containing a biopolymer array and each being accessible through its own port. Multiple fluid samples are introduced into respective chambers through a port of each such that the fluid samples contact respective arrays. A binding pattern of the arrays is observed. An apparatus and kit useful in such methods, are also provided.
摘要翻译：用多个生物聚合物阵列测试多个流体样品的方法。将盖组装到承载在第一侧上的连续基底，多个阵列，每个阵列具有连接到基底的多个生物聚合区域，使得盖和基底一起形成多个腔室，每个腔室包含生物聚合物阵列，并且每个腔室可通过自己的港口多个流体样品通过各自的端口引入相应的室中，使得流体样品与相应的阵列接触。观察到阵列的绑定模式。还提供了一种在这些方法中有用的装置和试剂盒。

5. 发明授权

US07252938B2 Methods and devices for producing a polymer at a location of a substrate 失效
标题翻译：在基材的位置处制备聚合物的方法和装置
公开(公告)号：US07252938B2
公开(公告)日：2007-08-07
申请号：US10374857
申请日：2003-02-25
申请人： Eric M. Leproust , Douglas A. Amorese
发明人： Eric M. Leproust , Douglas A. Amorese
IPC分类号： C12Q1/00 , C12Q1/68 , C12M1/36 , C12M3/00 , G01N33/53 , G01N33/566 , G01N33/543 , G01N33/551 , G01N33/544 , G01N21/00 , G01N21/75 , G01N21/76
CPC分类号： B01J19/0046 , B01J2219/00378 , B01J2219/00576 , B01J2219/00691 , B01J2219/00693 , B01J2219/00722 , B01J2219/00725 , C40B40/06 , C40B40/10 , C40B60/14 , Y10S436/807 , Y10S436/809
摘要： Methods and devices for producing a polymer at a location of a substrate are provided. In the subject methods, a fluid droplet containing a first monomer labeled with a first detectable label is deposited from a fluid deposition device onto a location of a substrate surface having a second monomer labeled with a second detectable label. The first and second detectable labels are then detected to determine any misalignment between the fluid deposition device and the location of the substrate surface during deposition. Also provided are algorithms that perform the subject methods, as well as fluid deposition devices that include the subject algorithms. The subject invention also includes arrays produced according to the subject methods and kits that include the subject arrays.
摘要翻译：提供了在基板的位置处制造聚合物的方法和装置。在本发明方法中，将含有用第一可检测标记物标记的第一单体的流体液滴从流体沉积装置沉积到具有用第二可检测标记物标记的第二单体的基底表面的位置。然后检测第一和第二可检测标记以确定在沉积期间流体沉积装置与基底表面的位置之间的任何未对准。还提供了执行主题方法的算法，以及包括主题算法的流体沉积设备。本发明还包括根据主题方法和包括主题阵列的试剂盒制备的阵列。

6. 发明授权

US07070932B2 Methods and devices for detecting printhead misalignment of an in situ polymeric array synthesis device 失效
标题翻译：用于检测原位聚合物阵列合成装置的打印头未对准的方法和装置
公开(公告)号：US07070932B2
公开(公告)日：2006-07-04
申请号：US10374307
申请日：2003-02-25
申请人： Eric M. Leproust , Douglas A. Amorese , Mel N. Kronick
发明人： Eric M. Leproust , Douglas A. Amorese , Mel N. Kronick
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6837 , C12Q2525/173
摘要： Methods and devices for detecting deposition unit misalignment, e.g., printhead misalignment, of an in situ polymeric, e.g., a nucleic acid, array synthesis device are provided. In accordance with the subject methods, at least one test probe feature is synthesized on a substrate using an in situ polymeric array, e.g., nucleic acid array or protein array, synthesis device. The at least one test probe feature is then contacted with at least two different distinguishably labeled targets, e.g., target nucleic acids. The binding of the targets to the at least one test probe feature is then evaluated to detect any misalignment, e.g., deposition unit or pulse jet misalignments, of the synthesis device. Also provided are substrates having at least one test probe feature and at least one polymeric array, as well as methods of using the substrates in array assays. Also included are kits for use in practicing the subject methods.
摘要翻译：提供了用于检测原位聚合物例如核酸阵列合成装置的沉积单元未对准（例如，打印头未对准）的方法和装置。根据本发明的方法，使用原位聚合物阵列，例如核酸阵列或蛋白质阵列，合成装置，在底物上合成至少一种测试探针特征。然后将至少一个测试探针特征与至少两个不同的可区分标记的靶，例如靶核酸接触。然后评估目标与至少一个测试探针特征的结合以检测合成装置的任何未对准，例如沉积单元或脉冲射流未对准。还提供了具有至少一个测试探针特征和至少一个聚合物阵列的基底，以及在阵列测定中使用底物的方法。还包括用于实践主题方法的工具包。

7. 发明授权

US07011949B2 Methods and compositions for producing labeled probe nucleic acids for use in array based comparative genomic hybridization applications 失效
公开(公告)号：US07011949B2
公开(公告)日：2006-03-14
申请号：US10261405
申请日：2002-09-30
申请人： Douglas A. Amorese , Diane D. Ilsley
发明人： Douglas A. Amorese , Diane D. Ilsley
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q2521/101 , C12Q2563/107 , C12Q2565/501
摘要： Methods and compositions for producing labeled probe nucleic acids from genomic nucleic acid template are provided. In the subject methods, a conserved coding consensus region primer is employed to enzymatically generate a select set of labeled probe nucleic acids corresponding to coding regions of genes from a genomic template via a primer extension protocol. The subject methods find use in a variety of different applications, and are particularly suited for use in the preparation of labeled probe nucleic acids for use in array based comparative genomic hybridization applications. Also provided are kits for use in practicing the subject methods.

8. 发明授权

US06682702B2 Apparatus and method for simultaneously conducting multiple chemical reactions 有权
标题翻译：同时进行多次化学反应的装置和方法
公开(公告)号：US06682702B2
公开(公告)日：2004-01-27
申请号：US09938909
申请日：2001-08-24
申请人： Phillip W. Barth , Douglas A. Amorese , Carol T. Schembri
发明人： Phillip W. Barth , Douglas A. Amorese , Carol T. Schembri
IPC分类号： B01L300
CPC分类号： G01N35/028 , G01N2035/00158 , Y10T436/25 , Y10T436/25375 , Y10T436/255
摘要： A method and apparatus for simultaneously conducting multiple chemical reactions combine a test sample with a chemical reactant in a plurality of closed reaction chambers to produce reaction products. The method comprises assembling a plate having the test sample in a plurality of spatially arranged wells with a microarray of similarly spatially arranged surface bound chemical reactants to form the sealed apparatus having the plurality of closed reaction chambers. The apparatus is sealed such that it is gas, liquid and/or fluid tight. The seal may be accomplished with a flexible array substrate or a flexible gasket, and one or more of mechanical clamps, external fluid pressure, radiation, heat, vacuum and an adhesive. The sealed apparatus can be subjected to various reaction conditions, such as intense mechanical agitation and a controlled temperature environment. A kit comprises one or more of the elements of the apparatus.
摘要翻译：用于同时进行多个化学反应的方法和装置将测试样品与多个封闭反应室中的化学反应物组合以产生反应产物。该方法包括将具有测试样品的板组装在具有类似空间布置的表面结合的化学反应物的微阵列的多个空间排列的孔中，以形成具有多个封闭反应室的密封装置。该装置被密封，使得其是气体，液体和/或流体密封的。密封可以用柔性阵列基底或柔性垫片以及机械夹具，外部流体压力，辐射，热量，真空和粘合剂中的一种或多种完成。密封装置可以经受各种反应条件，例如强烈的机械搅拌和受控的温度环境。套件包括装置的一个或多个元件。

9. 发明授权

US06399394B1 Testing multiple fluid samples with multiple biopolymer arrays 失效
标题翻译：用多个生物聚合物阵列测试多个流体样品
公开(公告)号：US06399394B1
公开(公告)日：2002-06-04
申请号：US09343645
申请日：1999-06-30
申请人： SueAnn C. Dahm , Arthur Schleifer , Carol T. Schembri , Douglas A. Amorese
发明人： SueAnn C. Dahm , Arthur Schleifer , Carol T. Schembri , Douglas A. Amorese
IPC分类号： G01N110
CPC分类号： B01L3/5025 , B01L3/5027 , B01L9/52 , B01L2200/027 , B01L2200/0689 , B01L2300/044 , B01L2300/0636 , B01L2300/0803 , B01L2300/0816 , B01L2300/0877 , B01L2400/0487 , Y10T436/2575
摘要： A method of testing multiple fluid samples with multiple biopolymer arrays. A cover is assembled to a contiguous substrate carrying on a first side, multiple arrays each with multiple regions of biopolymers linked to the substrate, such that the cover and the substrate together form a plurality of chambers each containing a biopolymer array and each being accessible through its own port. Multiple fluid samples are introduced into respective chambers through a port of each such that the fluid samples contact respective arrays. A binding pattern of the arrays is observed. An apparatus and kit useful in such methods, are also provided.
摘要翻译：用多个生物聚合物阵列测试多个流体样品的方法。将盖组装到承载在第一侧上的连续基底，多个阵列，每个阵列具有连接到基底的多个生物聚合区域，使得盖和基底一起形成多个腔室，每个腔室包含生物聚合物阵列，并且每个腔室可通过自己的港口多个流体样品通过各自的端口引入相应的室中，使得流体样品与相应的阵列接触。观察到阵列的绑定模式。还提供了一种在这些方法中有用的装置和试剂盒。

10. 发明授权

US5064519A Neutral and positively charged dyes for electrophoresis sample loading solutions 失效
标题翻译：中性和带正电荷的染料用于电泳样品加载溶液
公开(公告)号：US5064519A
公开(公告)日：1991-11-12
申请号：US546186
申请日：1990-06-29
申请人： George Tice, Jr. , Douglas A. Amorese
发明人： George Tice, Jr. , Douglas A. Amorese
IPC分类号： G01N27/447
CPC分类号： G01N27/44743
摘要： A method of electrophoretically separating bioorganic molecules is disclosed. The method uses a slab electrophoresis gel and positively or neutrally charged dyes to prevent the dyes from interfering with the gel readout.

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