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1. 发明授权

US5853993A Signal enhancement method and kit 失效
标题翻译：信号增强方法和试剂盒
公开(公告)号：US5853993A
公开(公告)日：1998-12-29
申请号：US735381
申请日：1996-10-21
申请人： Douglas J. Dellinger , SueAnn C. Dahm , Mark A. Troll
发明人： Douglas J. Dellinger , SueAnn C. Dahm , Mark A. Troll
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , C12N15/00
CPC分类号： C12Q1/682
摘要： The invention discloses and claims a signal amplification method for detecting a target nucleic acid analyte having a homopolymeric region and a target sequence. The method comprises (a) contacting an analyte under hybridizing conditions with a multiplicity of reporter probes, each probe including a signal region and an oligonucleotide sequence which is complementary to, and capable of forming a stable hybrid with the analyte homopolymeric region, whereby the hybridization of multiple reporter probes to the homopolymeric region provides for signal amplification; and (b) forming an analyte:capture probe hybrid by contacting the analyte target sequence with a capture probe under hybridizing conditions.
摘要翻译：本发明公开了一种用于检测具有均聚区和靶序列的靶核酸分析物的信号放大方法。该方法包括（a）在杂交条件下将分析物与多种报道探针接触，每个探针包括信号区和寡核苷酸序列，其与分析物均聚区相互补充并能够形成稳定的杂交，由此杂交的多个报道探针到均聚区域提供信号放大; 和（b）通过在杂交条件下使分析物靶序列与捕获探针接触来形成分析物：捕获探针杂交体。

2. 发明授权

US6103474A Hybridization assay signal enhancement 失效
标题翻译：杂交测定信号增强
公开(公告)号：US6103474A
公开(公告)日：2000-08-15
申请号：US183619
申请日：1998-10-30
申请人： Douglas J. Dellinger , SueAnn C. Dahm , Diane D. Ilsley , Robert A. Ach , Mark A. Troll
发明人： Douglas J. Dellinger , SueAnn C. Dahm , Diane D. Ilsley , Robert A. Ach , Mark A. Troll
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , C12N15/00
CPC分类号： C12Q1/682
摘要： A signal amplification method for detecting a target nucleic acid analyte having a homopolymeric region and a target sequence includes steps of: contacting an analyte under hybridizing conditions with a multiplicity of reporter probes, each reporter probe including a signal region and an oligonucleotide sequence which is complementary to and capable of forming a stable hybrid with the analyte homopolymeric region to form an analyte:reporter probe hybrid; and forming an analyte:capture probe hybrid by contacting the analyte target sequence with a capture probe under hybridizing conditions. The analyte:reporter probe hybrid may formed prior to contacting the analyte target sequence with the capture probe, so the result of contacting the analyte target sequence with the capture probe results in formation of an analyte:reporter probe:capture probe complex. The analyte:capture probe hybrid may be immobilized on a solid generally planar surface in an array format. Multiple reporter probes may form triple helix structures for further signal enhancement. Multiply-labeled hairpin reporter probes may be employed according to the invention. Also, a kit for carrying out the invention includes one or more capture probes immobilized on a surface, reporter probes each having a signal region and a sequence for binding analyte homopolymer regions, and, optionally, reagents for addition of homopolymer regions to nucleic acid analytes and for carrying out the hybridization reaction.
摘要翻译：用于检测具有均聚区和靶序列的靶核酸分析物的信号扩增方法包括以下步骤：在杂交条件下与多个报道探针接触分析物，每个报道探针包括信号区和互补的寡核苷酸序列并且能够与分析物均聚区形成稳定的杂交体以形成分析物：报道探针杂交体; 并通过在杂交条件下将分析物靶序列与捕获探针接触来形成分析物：捕获探针杂交体。在将分析物靶序列与捕获探针接触之前可以形成分析物：报告物探针杂交体，因此将分析物靶序列与捕获探针接触的结果导致分析物的形成：报道探针：捕获探针复合物。分析物：捕获探针杂交体可以以阵列形式固定在固体大体上平坦的表面上。多个报道探针可以形成用于进一步信号增强的三重螺旋结构。根据本发明，可以使用多重标记的发夹报道探针。此外，用于实施本发明的试剂盒包括固定在表面上的一种或多种捕获探针，各自具有信号区域的报道探针和用于结合分析物均聚物区域的序列，以及任选的用于向核酸分析物加入均聚物区域的试剂并用于进行杂交反应。

3. 发明授权

US07537936B2 Method of testing multiple fluid samples with multiple biopolymer arrays 失效
标题翻译：用多个生物聚合物阵列测试多个流体样品的方法
公开(公告)号：US07537936B2
公开(公告)日：2009-05-26
申请号：US11136227
申请日：2005-05-23
申请人： SueAnn C. Dahm , Arthur Schleifer , Carol T. Schembri , Douglas A. Amorese
发明人： SueAnn C. Dahm , Arthur Schleifer , Carol T. Schembri , Douglas A. Amorese
IPC分类号： G01N30/00
CPC分类号： B01L3/502 , B01L3/5025 , B01L7/00 , B01L2200/027 , B01L2200/0689 , B01L2300/0636 , B01L2300/0803 , B01L2300/0822 , B01L2300/0877 , B01L2400/0487 , Y10T436/2575
摘要： A method of testing multiple fluid samples with multiple biopolymer arrays. A cover is assembled to a contiguous substrate carrying on a first side, multiple arrays each with multiple regions of biopolymers linked to the substrate, such that the cover and the substrate together form a plurality of chambers each containing a biopolymer array and each being accessible through its own port. Multiple fluid samples are introduced into respective chambers through a port of each such that the fluid samples contact respective arrays. A binding pattern of the arrays is observed. An apparatus and kit useful in such methods, are also provided.
摘要翻译：用多个生物聚合物阵列测试多个流体样品的方法。将盖组装到承载在第一侧上的连续基底，多个阵列，每个阵列具有连接到基底的多个生物聚合区域，使得盖和基底一起形成多个腔室，每个腔室包含生物聚合物阵列，并且每个腔室可通过自己的港口多个流体样品通过各自的端口引入相应的室中，使得流体样品与相应的阵列接触。观察到阵列的绑定模式。还提供了一种在这些方法中有用的装置和试剂盒。

4. 发明授权

US07247497B2 Testing multiple fluid samples with multiple biopolymer arrays 有权
标题翻译：用多个生物聚合物阵列测试多个流体样品
公开(公告)号：US07247497B2
公开(公告)日：2007-07-24
申请号：US10160620
申请日：2002-05-31
申请人： SueAnn C. Dahm , Arthur Schleifer , Carol T. Schembri , Douglas A. Amorese
发明人： SueAnn C. Dahm , Arthur Schleifer , Carol T. Schembri , Douglas A. Amorese
IPC分类号： G01N30/00
CPC分类号： B01L3/5025 , B01L3/5027 , B01L9/52 , B01L2200/027 , B01L2200/0689 , B01L2300/044 , B01L2300/0636 , B01L2300/0803 , B01L2300/0816 , B01L2300/0877 , B01L2400/0487 , Y10T436/2575
摘要： A method of testing multiple fluid samples with multiple biopolymer arrays. A cover is assembled to a contiguous substrate carrying on a first side, multiple arrays each with multiple regions of biopolymers linked to the substrate, such that the cover and the substrate together form a plurality of chambers each containing a biopolymer array and each being accessible through its own port. Multiple fluid samples are introduced into respective chambers through a port of each such that the fluid samples contact respective arrays. A binding pattern of the arrays is observed. An apparatus and kit useful in such methods, are also provided.
摘要翻译：用多个生物聚合物阵列测试多个流体样品的方法。将盖组装到承载在第一侧上的连续基底，多个阵列，每个阵列具有连接到基底的多个生物聚合区域，使得盖和基底一起形成多个腔室，每个腔室包含生物聚合物阵列，并且每个腔室可通过自己的港口多个流体样品通过各自的端口引入相应的室中，使得流体样品与相应的阵列接触。观察到阵列的绑定模式。还提供了一种在这些方法中有用的装置和试剂盒。

5. 发明授权

US06399394B1 Testing multiple fluid samples with multiple biopolymer arrays 失效
标题翻译：用多个生物聚合物阵列测试多个流体样品
公开(公告)号：US06399394B1
公开(公告)日：2002-06-04
申请号：US09343645
申请日：1999-06-30
申请人： SueAnn C. Dahm , Arthur Schleifer , Carol T. Schembri , Douglas A. Amorese
发明人： SueAnn C. Dahm , Arthur Schleifer , Carol T. Schembri , Douglas A. Amorese
IPC分类号： G01N110
CPC分类号： B01L3/5025 , B01L3/5027 , B01L9/52 , B01L2200/027 , B01L2200/0689 , B01L2300/044 , B01L2300/0636 , B01L2300/0803 , B01L2300/0816 , B01L2300/0877 , B01L2400/0487 , Y10T436/2575
摘要： A method of testing multiple fluid samples with multiple biopolymer arrays. A cover is assembled to a contiguous substrate carrying on a first side, multiple arrays each with multiple regions of biopolymers linked to the substrate, such that the cover and the substrate together form a plurality of chambers each containing a biopolymer array and each being accessible through its own port. Multiple fluid samples are introduced into respective chambers through a port of each such that the fluid samples contact respective arrays. A binding pattern of the arrays is observed. An apparatus and kit useful in such methods, are also provided.
摘要翻译：用多个生物聚合物阵列测试多个流体样品的方法。将盖组装到承载在第一侧上的连续基底，多个阵列，每个阵列具有连接到基底的多个生物聚合区域，使得盖和基底一起形成多个腔室，每个腔室包含生物聚合物阵列，并且每个腔室可通过自己的港口多个流体样品通过各自的端口引入相应的室中，使得流体样品与相应的阵列接触。观察到阵列的绑定模式。还提供了一种在这些方法中有用的装置和试剂盒。

6. 发明授权

US6110682A Signal enhancement method and kit 失效
标题翻译：信号增强方法和试剂盒
公开(公告)号：US6110682A
公开(公告)日：2000-08-29
申请号：US201674
申请日：1998-11-30
申请人： Douglas J. Dellinger , SueAnn C. Dahm , Mark A. Troll
发明人： Douglas J. Dellinger , SueAnn C. Dahm , Mark A. Troll
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , C12N15/00
CPC分类号： C12Q1/682
摘要： The invention discloses and claims a signal amplification method for detecting a target nucleic acid analyte having a homopolymeric region and a target sequence. The method comprises (a) contacting an analyte under hybridizing conditions with a multiplicity of reporter probes, each probe including a signal region and an oligonucleotide sequence which is complementary to, and capable of forming a stable hybrid with the analyte homopolymeric region; and (b) forming an analyte:capture probe hybrid by contacting the analyte target sequence with a capture probe under hybridizing conditions.
摘要翻译：本发明公开了一种用于检测具有均聚区和靶序列的靶核酸分析物的信号放大方法。该方法包括（a）在杂交条件下将分析物与多个报道探针接触，每个探针包括与分析物均聚区互补并能够与分析物均聚区形成稳定杂交的信号区和寡核苷酸序列; 和（b）通过在杂交条件下使分析物靶序列与捕获探针接触来形成分析物：捕获探针杂交体。

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