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1. 发明授权

US07070932B2 Methods and devices for detecting printhead misalignment of an in situ polymeric array synthesis device 失效
标题翻译：用于检测原位聚合物阵列合成装置的打印头未对准的方法和装置
公开(公告)号：US07070932B2
公开(公告)日：2006-07-04
申请号：US10374307
申请日：2003-02-25
申请人： Eric M. Leproust , Douglas A. Amorese , Mel N. Kronick
发明人： Eric M. Leproust , Douglas A. Amorese , Mel N. Kronick
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6837 , C12Q2525/173
摘要： Methods and devices for detecting deposition unit misalignment, e.g., printhead misalignment, of an in situ polymeric, e.g., a nucleic acid, array synthesis device are provided. In accordance with the subject methods, at least one test probe feature is synthesized on a substrate using an in situ polymeric array, e.g., nucleic acid array or protein array, synthesis device. The at least one test probe feature is then contacted with at least two different distinguishably labeled targets, e.g., target nucleic acids. The binding of the targets to the at least one test probe feature is then evaluated to detect any misalignment, e.g., deposition unit or pulse jet misalignments, of the synthesis device. Also provided are substrates having at least one test probe feature and at least one polymeric array, as well as methods of using the substrates in array assays. Also included are kits for use in practicing the subject methods.
摘要翻译：提供了用于检测原位聚合物例如核酸阵列合成装置的沉积单元未对准（例如，打印头未对准）的方法和装置。根据本发明的方法，使用原位聚合物阵列，例如核酸阵列或蛋白质阵列，合成装置，在底物上合成至少一种测试探针特征。然后将至少一个测试探针特征与至少两个不同的可区分标记的靶，例如靶核酸接触。然后评估目标与至少一个测试探针特征的结合以检测合成装置的任何未对准，例如沉积单元或脉冲射流未对准。还提供了具有至少一个测试探针特征和至少一个聚合物阵列的基底，以及在阵列测定中使用底物的方法。还包括用于实践主题方法的工具包。

2. 发明申请

US20110230371A1 Chemical Arrays and Methods of Using the Same 有权
标题翻译：化学数组及其使用方法
公开(公告)号：US20110230371A1
公开(公告)日：2011-09-22
申请号：US13116998
申请日：2011-05-26
申请人： Mel N. Kronick , Eric M. Leproust
发明人： Mel N. Kronick , Eric M. Leproust
IPC分类号： C40B40/04 , C40B50/14
CPC分类号： C40B30/04 , G01N33/54366
摘要： Methods and compositions for generating mixtures of product molecules from an initial chemical array are provided. In the subject methods, a chemical array of surface immobilized first moieties is subjected to cleavage conditions such that a composition of solution phase first moieties is produced. The resultant composition of solution phase first moieties is then contacted with one or more reactants to produce a mixture of product molecules that are different from the first moieties. Also provided are the arrays employed in the subject methods and kits for practicing the subject methods.
摘要翻译：提供了从初始化学阵列产生产物分子的混合物的方法和组合物。在本发明方法中，表面固定的第一部分的化学阵列经受切割条件，使得产生溶液相第一部分的组合物。然后将所得到的溶液相第一部分的组合物与一种或多种反应物接触以产生不同于第一部分的产物分子的混合物。还提供了用于实践本发明方法的主题方法和试剂盒中使用的阵列。

3. 发明授权

US10017879B2 Chemical arrays and methods of using the same 有权
公开(公告)号：US10017879B2
公开(公告)日：2018-07-10
申请号：US13116998
申请日：2011-05-26
申请人： Mel N. Kronick , Eric M. Leproust
发明人： Mel N. Kronick , Eric M. Leproust
IPC分类号： C40B50/18 , C40B30/04 , G01N33/543
CPC分类号： C40B30/04 , G01N33/54366
摘要： Methods and compositions for generating mixtures of product molecules from an initial chemical array are provided. In the subject methods, a chemical array of surface immobilized first moieties is subjected to cleavage conditions such that a composition of solution phase first moieties is produced. The resultant composition of solution phase first moieties is then contacted with one or more reactants to produce a mixture of product molecules that are different from the first moieties. Also provided are the arrays employed in the subject methods and kits for practicing the subject methods.

4. 发明授权

US07977119B2 Chemical arrays and methods of using the same 有权
标题翻译：化学数组及其使用方法
公开(公告)号：US07977119B2
公开(公告)日：2011-07-12
申请号：US11008384
申请日：2004-12-08
申请人： Mel N. Kronick , Eric M. Leproust
发明人： Mel N. Kronick , Eric M. Leproust
IPC分类号： G01N33/53 , G01N33/532 , G01N33/543 , G01N33/566 , C07K5/00 , C07K14/00 , C07K17/00
CPC分类号： C40B30/04 , G01N33/54366
摘要： Methods and compositions for generating mixtures of product molecules from an initial chemical array are provided. In the subject methods, a chemical array of surface immobilized first moieties is subjected to cleavage conditions such that a composition of solution phase first moieties is produced. The resultant composition of solution phase first moieties is then contacted with one or more reactants to produce a mixture of product molecules that are different from the first moieties. Also provided are the arrays employed in the subject methods and kits for practicing the subject methods.
摘要翻译：提供了从初始化学阵列产生产物分子的混合物的方法和组合物。在本发明方法中，表面固定的第一部分的化学阵列经受切割条件，使得产生溶液相第一部分的组合物。然后将所得到的溶液相第一部分的组合物与一种或多种反应物接触以产生不同于第一部分的产物分子的混合物。还提供了用于实践本发明方法的主题方法和试剂盒中使用的阵列。

5. 发明授权

US07588889B2 Chemical arrays and methods of using the same 失效
标题翻译：化学数组及其使用方法
公开(公告)号：US07588889B2
公开(公告)日：2009-09-15
申请号：US11008603
申请日：2004-12-08
申请人： Paul K. Wolber , Robert H. Kincaid , Douglas Amorese , Diane Ilsley-Tyree , Andrew S. Atwell , Mel N. Kronick , Eric M. Leproust
发明人： Paul K. Wolber , Robert H. Kincaid , Douglas Amorese , Diane Ilsley-Tyree , Andrew S. Atwell , Mel N. Kronick , Eric M. Leproust
IPC分类号： C12Q1/68 , C12M1/00 , C07H21/04
CPC分类号： C12Q1/6883 , B01J2219/00497 , B01J2219/00572 , B01J2219/00574 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00617 , B01J2219/00626 , B01J2219/00659 , B01J2219/00675 , B01J2219/00722 , B82Y30/00 , C12Q1/6806 , C12Q1/6837 , C12Q1/6876 , C12Q2600/158 , C40B40/06 , C40B50/14 , C40B70/00 , C12Q2565/537 , C12Q2525/161 , C12Q2565/525 , C12Q2525/131 , C12Q2521/301
摘要： Methods and compositions for generating mixtures of product molecules from an initial chemical array are provided. In the subject methods, a chemical array of surface immobilized first moieties is subjected to cleavage conditions such that a composition of solution phase first moieties is produced. The resultant composition of solution phase first moieties is then contacted with one or more reactants to produce a mixture of product molecules that are different from the first moieties. Also provided are the arrays employed in the subject methods and kits for practicing the subject methods.
摘要翻译：提供了从初始化学阵列产生产物分子的混合物的方法和组合物。在本发明方法中，表面固定的第一部分的化学阵列经受切割条件，使得产生溶液相第一部分的组合物。然后将所得到的溶液相第一部分的组合物与一种或多种反应物接触以产生不同于第一部分的产物分子的混合物。还提供了用于实践本发明方法的主题方法和试剂盒中使用的阵列。

6. 发明授权

US06251685B1 Readout method for molecular biological electronically addressable arrays 失效
标题翻译：分子生物电子寻址阵列的读出方法
公开(公告)号：US06251685B1
公开(公告)日：2001-06-26
申请号：US09252208
申请日：1999-02-18
申请人： Andreas Nikolaus Dorsel , Mel N. Kronick , Gary B. Gordon
发明人： Andreas Nikolaus Dorsel , Mel N. Kronick , Gary B. Gordon
IPC分类号： G01N27327
CPC分类号： G01N21/66 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00628 , B01J2219/00635 , B01J2219/00637 , B01J2219/00653 , B01J2219/00659 , B01J2219/00722 , G01N21/76 , Y10T436/112499 , Y10T436/2575
摘要： A method for reading out data from microlocations of a microelectronic array involves activating multiple microlocations in parallel and simultaneously detecting the responses from the activated microlocations to determine concentrations of molecular biological material at each microlocation. In a preferred embodiment, the microelectronic array includes electronically addressable electrodes at each microlocation which can be individually activated via a control system. An electrochemiluminescent detection technique is used to detect the presence and determine the concentration of bound molecular biological material that is located at each microlocation. Electrochemiluminescent material is utilized because it gives off light when excited by an applied electrical field. With an addressable microelectronic array, electrical fields can be applied to various combinations of microlocations simultaneously to allow readout of several microlocations in parallel. This is in contrast to the laser-based readout approach which applies activation energy to one microlocation at a time by impacting each microlocation with a single laser system. Reading out multiple microlocations simultaneously in accordance with the invention can produce significant time savings in large arrays.
摘要翻译：从微电子阵列的微位置读出数据的方法包括平行地激活多个微位点，同时检测来自活化微位点的响应，以确定每个微位置处的分子生物材料的浓度。在优选实施例中，微电子阵列包括每个微位置处的可电子寻址电极，其可以经由控制系统单独激活。电化学发光检测技术用于检测位于每个微位置的结合分子生物材料的存在和确定浓度。使用电化学发光材料，因为当通过施加的电场激发时它发出光。利用可寻址的微电子阵列，电场可以同时应用于微位置的各种组合，以允许并行读出几个微位置。这与基于激光的读出方法相反，该方法通过用单个激光系统冲击每个微位置，一次将激活能量施加到一个微位置。根据本发明同时读出多个微位置可以在大阵列中产生显着的时间节省。

7. 发明授权

US6054266A Nucleic acid detection with separation 失效
标题翻译：分离核酸检测
公开(公告)号：US6054266A
公开(公告)日：2000-04-25
申请号：US276139
申请日：1988-11-23
申请人： Mel N. Kronick , Douglas H. Keith , Lincoln J. McBride , Norman M. Whiteley , Michael W. Hunkapiller
发明人： Mel N. Kronick , Douglas H. Keith , Lincoln J. McBride , Norman M. Whiteley , Michael W. Hunkapiller
IPC分类号： G01N33/50 , C12Q1/68 , G01N30/88 , G01N33/58 , C07H21/04
CPC分类号： C12Q1/6813 , C12Q1/6804 , C12Q1/6823
摘要： Nucleic acid sequences are detected by a multi-step process, involving labeling sample nucleic acid sequences, duplexing the labeled sample with a probe having a coupling element, immobilizing all of the duplexed probe and target sequence and unduplexed probe, separating specifically immobilized nucleic acid from free and non-specifically immobilized nucleic acid, releasing specifically immobilized nucleic acid, and detecting the presence of the sequence of interest by means of the label. The labeled sequence may be characterized by sizing, e.g. electrophoresis. The method provides for a sensitive and rapid means for accurate detection of sequences of interest in a wide variety of situations.
摘要翻译：通过多步法检测核酸序列，包括标记样品核酸序列，用具有偶联元件的探针双链化标记的样品，固定所有双链体探针和靶序列和未折射的探针，将特异性固定的核酸与游离和非特异性固定化的核酸，释放特异性固定化的核酸，并通过标记物检测感兴趣的序列的存在。标记序列的特征可以是尺寸，例如电泳。该方法提供用于在各种情况下精确检测感兴趣序列的敏感且快速的方法。

8. 发明授权

US5093245A Labeling by simultaneous ligation and restriction 失效
标题翻译：同时标注和限制标签
公开(公告)号：US5093245A
公开(公告)日：1992-03-03
申请号：US148757
申请日：1988-01-26
申请人： Douglas H. Keith , Mel N. Kronick , Lincoln J. McBride , Norman M. Whiteley
发明人： Douglas H. Keith , Mel N. Kronick , Lincoln J. McBride , Norman M. Whiteley
IPC分类号： C07H21/04 , C12N15/09 , C12Q1/68 , C12Q1/70 , G01N33/50
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/6827 , C12Q1/683 , C12Q1/6855 , C12Q1/6858 , Y10S435/81
摘要： Termini of restricted double-stranded DNA fragments are modified by ligating the fragments with terminal phosphate-free double-stranded oligonucleotides having a complementary terminus in the presence of a restriction enzyme and a ligase, where joining of the complementary ends results in loss of the restriction enzyme recognition sequence.

9. 发明授权

US07642097B2 Manufacture and use of non-standard size microarray slides 失效
标题翻译：制造和使用非标尺寸的微阵列幻灯片
公开(公告)号：US07642097B2
公开(公告)日：2010-01-05
申请号：US12163751
申请日：2008-06-27
申请人： Mel N. Kronick , Barnett E. Saunders , Matthew J. Marton , John S. Hinchcliffe , Dianne Rees , Carol T. Schembri
发明人： Mel N. Kronick , Barnett E. Saunders , Matthew J. Marton , John S. Hinchcliffe , Dianne Rees , Carol T. Schembri
IPC分类号： B01L9/00
CPC分类号： B01L9/52 , B01J2219/00531 , B01J2219/00547 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00662 , B01L3/508 , B01L2300/0636 , B01L2300/0819 , B01L2300/0822 , G02B21/34 , Y10T436/25 , Y10T436/2575
摘要： Methods and devices are disclosed for microarray analysis. In one embodiment a method is disclosed for processing a non-standard size slide having an array of chemical compounds attached to a surface of the slide. A sample is exposed to the surface of the non-standard size slide wherein components in the sample bind to the chemical compounds on the surface of the slide. The sample and the slide are incubated under conditions for carrying out the binding reactions, and the surface of the non-standard size slide is examined for the results of the binding reactions. Prior to the exposing step or the incubating step or the examining step, the non-standard size slide is placed into a slide holder comprising a slide-holding section a slide-holding section adapted to dispose the non-standard size slide to a processing instrument in a manner similar to that for a standard size slide. The non-standard size slide may also include an identifier such as a bar code.
摘要翻译：公开了用于微阵列分析的方法和装置。在一个实施方案中，公开了一种用于处理具有附着到载玻片的表面的化学化合物阵列的非标尺寸载玻片的方法。将样品暴露于非标准尺寸载玻片的表面，其中样品中的组分与载玻片表面上的化合物结合。样品和载玻片在进行结合反应的条件下孵育，并检查非标准大小载玻片的表面结合反应的结果。在暴露步骤或孵化步骤或检查步骤之前，将非标准尺寸的载玻片放置在载玻片保持器中，滑动保持器包括滑动保持部分，该滑动保持部分适于将非标准尺寸滑块配置到处理器械以类似于标准尺寸幻灯片的方式。非标准尺寸的幻灯片还可以包括诸如条形码的标识符。

10. 发明申请

US20080261826A1 Manufacture And Use Of Non-Standard Size Microarray Slides 失效
标题翻译：制造和使用非标准尺寸的微阵列幻灯片
公开(公告)号：US20080261826A1
公开(公告)日：2008-10-23
申请号：US12163751
申请日：2008-06-27
申请人： Mel N. Kronick , Barnett E. Saunders , Matthew J. Marton , John S. Hinchcliffe , Dianne Rees , Carol T. Schembri
发明人： Mel N. Kronick , Barnett E. Saunders , Matthew J. Marton , John S. Hinchcliffe , Dianne Rees , Carol T. Schembri
IPC分类号： C40B30/04 , C40B50/14 , C40B40/00
CPC分类号： B01L9/52 , B01J2219/00531 , B01J2219/00547 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00662 , B01L3/508 , B01L2300/0636 , B01L2300/0819 , B01L2300/0822 , G02B21/34 , Y10T436/25 , Y10T436/2575
摘要： Methods and devices are disclosed for microarray analysis. In one embodiment a method is disclosed for processing a non-standard size slide having an array of chemical compounds attached to a surface of the slide. A sample is exposed to the surface of the non-standard size slide wherein components in the sample bind to the chemical compounds on the surface of the slide. The sample and the slide are incubated under conditions for carrying out the binding reactions, and the surface of the non-standard size slide is examined for the results of the binding reactions. Prior to the exposing step or the incubating step or the examining step, the non-standard size slide is placed into a slide holder comprising a slide-holding section a slide-holding section adapted to dispose the non-standard size slide to a processing instrument in a manner similar to that for a standard size slide. The non-standard size slide may also include an identifier such as a bar code.
摘要翻译：公开了用于微阵列分析的方法和装置。在一个实施方案中，公开了一种用于处理具有附着到载玻片的表面的化学化合物阵列的非标尺寸载玻片的方法。将样品暴露于非标准尺寸载玻片的表面，其中样品中的组分与载玻片表面上的化合物结合。将样品和载玻片在进行结合反应的条件下孵育，并检查非标准尺寸载玻片的表面的结合反应结果。在暴露步骤或孵化步骤或检查步骤之前，将非标准尺寸的载玻片放置在载玻片保持器中，滑动保持器包括滑动保持部分，该滑动保持部分适于将非标准尺寸滑块配置到处理器械以类似于标准尺寸幻灯片的方式。非标准尺寸的幻灯片还可以包括诸如条形码的标识符。

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